Collection and preservation of eDNA from marine water samples v1

2021 ◽  
Author(s):  
Ana Ramón-Laca ◽  
Abigail Wells ◽  
Linda Park
Keyword(s):  
Water Samples ◽  
Room Temperature ◽  
Marine Water ◽  
Water Collection ◽  
Lysis Buffer

This protocol is designed for water collection from Niskin bottles and filtration at sea using reusable filter cups. Aim: to collect and filter 2.5 L of water at each depth from each CTD cast and preserved the filter at room temperature in lysis buffer

Collection and preservation of eDNA from marine water samples v1

2021 ◽  
Author(s):  
Ana Laca ◽  
Abigail Wells ◽  
Linda Park
Keyword(s):  
Water Samples ◽  
Room Temperature ◽  
Marine Water ◽  
Water Collection

This protocol is designed for water collection from Niskin bottles and filtration at sea using reusable filter cups. Aim: to collect and filter 2.5 L of water at each depth from each CTD cast and preserved the filter at room temperature.

An improved device with an affinity membrane to collect depth specific contamination free water for environmental assessment

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 662-669 ◽  
Author(s):  
Sweta Binod Kumar ◽  
Hardik Trivedi ◽  
Narshibhai Rameshbhai Baraiya ◽  
Soumya Haldar
Keyword(s):  
Environmental Assessment ◽  
Water Samples ◽  
Free Water ◽  
Marine Water ◽  
Sampling Device ◽  
Affinity Membrane

The prime requirement for marine water studies is a competent sampling device that can collect water samples perfectly without any contamination to avoid false analysis.

scholarly journals The effect of storage on the coliform and Bacterium coli counts of water samples

1953 ◽  
Vol 51 (4) ◽  
pp. 559-571 ◽  
Author(s):  
Keyword(s):  
Water Samples ◽  
Room Temperature ◽  
Refrigerator Temperature ◽  
Significant Change

This investigation has been concerned with the changes that occur in the coliform and faecal coli content of water samples on storage at room and refrigerator temperatures for 6 hr. as compared with those occurring after 24 hr. storage.The examination was conducted by a 70-tube method using twofold diminishing volumes.The percentages of samples showing a significant change corresponding to at least a doubling or halving of the coliform content on storage were: (a) at room temperature after 6 hr. 24.7%, and after 24 hr. 38.3%; (b) at refrigerator temperature after 6 hr. 25.0%, and after 24 hr. 33.9%. The percentages of samples showing a significant change in the faecal coli content on storage were: (a) at room temperature after 6 hr. 18.7%, and after 24 hr. 34.6%; (b) at refrigerator temperature after 6 hr. 10.4%, and after 24 hr. 25.0%. The changes were preponderantly decreases and most of the large changes occurred after 24 hr.The investigation has shown that fewer changes occur after 6 hr. storage than after 24 hr. storage, and that storage for 6 hr. at refrigerator temperature is preferable to storage for 6 hr. at room temperature. Even so, some samples show significant changes after 6 hr. storage at refrigerator temperature; the coliform content is significantly altered in 25% of samples and the faecal coli content in 10% of samples.Samples of water should, therefore, be examined as soon as possible after collection, certainly within 6 hr., and during the period of transport to the laboratory they should be kept cold, if possible by ice.

Copper complexation capacity of marine water samples from southern California

1980 ◽  
Vol 14 (12) ◽  
pp. 1482-1486 ◽  
Author(s):  
Richard F. Srna ◽  
Kenn S. Garrett ◽  
Sandra M. Miller ◽  
Alan B. Thum
Keyword(s):  
Water Samples ◽  
Southern California ◽  
Marine Water ◽  
Complexation Capacity ◽  
Copper Complexation

scholarly journals Self-Driven Pretreatment and Room-Temperature Storage of Water Samples for Virus Detection Using Enhanced Porous Superabsorbent Polymer (PSAP) Beads

2021 ◽  
Author(s):  
Wensi Chen ◽  
Ting Wang ◽  
Zeou Dou ◽  
Xing Xie
Keyword(s):  
Water Samples ◽  
Large Scale ◽  
Room Temperature ◽  
Low Cost ◽  
Virus Detection ◽  
Sample Pretreatment ◽  
Superabsorbent Polymer ◽  
Viral Pathogens ◽  
Untreated Wastewater ◽  
Degradation Rate Constant

Abstract The continuous emergence of infectious viral diseases has become a major threat to public health. To quantify viruses, proper handling of water samples is required to ensure the accuracy and reliability of the testing results. In this study, we develop enhanced porous superabsorbent polymer (PSAP) beads to pretreat and store water samples for virus detection. By applying PSAP beads to collect water samples, the viruses are captured and encapsulated inside the beads while undesired components are excluded. We have successfully demonstrated that the shelf life of the model virus can be effectively extended at room temperature (22°C) and elevated temperature (35°C). Both the infectivity level and genome abundance of the viruses are protected even in a complex medium like untreated wastewater. Under the tested conditions, the viral degradation rate constant can be reduced to more than 10 times using the PSAP beads. Therefore, the enhanced PSAP beads provide a low-cost and efficient sample pretreatment and storage method that is feasible and practicable for large-scale surveillance of viral pathogens in water samples.

scholarly journals AMMONIA AND NITRATE FLUCTUATIONS IN STORED WATER SAMPLES

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 484e-484
Author(s):  
D.C. Fare ◽  
C.H. Gilliam ◽  
G.J. Keever ◽  
J.T. Touchton
Keyword(s):  
Sulfuric Acid ◽  
Water Samples ◽  
Room Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Nitrate Concentrations

Water samples containing 0, 2.5, 10.0, or 20.0 ppm nitrate and ammonia were evaluated under 3 temperatures (0, 6, 20C) plus or minus sulfuric acid (36N) for changes in concentration. Ammonia and nitrate levels were measured 0, 1, 2, 4, 8, 16, 24, and 32 weeks after storing. Response to storage conditions was the same regardless of acid or concentration of ammonia or nitrate. Nitrate concentrations in the storage locations were similar for the first 2 weeks. Afterwards, treatments stored at room temperature fluctuated from initial standards. With ammonia, frozen samples had the greatest deviation from initial standards during the first 4 weeks. By week 24, ammonia samples stored at room temperature had exceeded acceptable deviations from the standards. Nitrate and ammonia samples held in refrigeration had the least fluctuation during the 32 week storage period.

scholarly journals Ca2+ removal from water by the use of Na-palygorskite for potential water softening

2021 ◽  
Author(s):  
Gianni Eleni ◽  
Panagopoulos Georgios ◽  
Katsanou Konstantina ◽  
Biniaris Alexandros
Keyword(s):  
Linear Form ◽  
Water Samples ◽  
Room Temperature ◽  
Low Cost ◽  
Water Hardness ◽  
Water Softening ◽  
Initial Concentration ◽  
Ph Values ◽  
Langmuir Isotherm Model ◽  
Ft Ir

Abstract Sodium-treated palygorskite (Na-Pal) sample was investigated for the sorption of Ca2+ ions with the aim of treating water hardness. The effective modification of the mineral with Na+ was verified by XRD and FT-IR techniques. Batch kinetic experiments in standard solutions were performed proving that 30 g/L of Na-Pal were highly satisfactory as the Ca2+ removal reached the 85% for 100 mg/L Ca2+ initial concentration, which is very promising for the softening of moderate or hard waters. The Ca2+ removal found to be pH and temperature independent, with high removal rates at room temperature and common pH values of water samples (pH 4–12), rendering these circumstances ideal for the low-cost maintenance of the procedure that took place within the first 5 min. The linear form of the Langmuir isotherm model expressed better (R2 = 1) the Ca2+ sorption, which means that takes place at specific homogeneous sites of Na-Pal. Thermodynamic analysis proved the non-spontaneous (positive ΔG0), physical, and exothermic nature (ΔH0 = −10.8197 kJ/mol) of the reaction, while the kinetic models proved the chemisorption of Ca2+ by Na-Pal.

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