Full plate & spot test plaque assays + PFU/mL calc. - aerobic bacteria v1

2021 ◽  
Author(s):  
Torben Sølbeck Rasmussen
Keyword(s):  
Plaque Assay ◽  
High Accuracy ◽  
Spot Test ◽  
Aerobic Bacteria

Protocol for plaque assays - either a full plate plaque assay that are more laborious but with high accuracy, or a spot test plaque assay that are for higher throughput but with less accuracy.

Full plate & spot test plaque assays + PFU/mL calc. - anaerobic/slow growing bacteria v1

2021 ◽  
Author(s):  
Torben Sølbeck Rasmussen
Keyword(s):  
Plaque Assay ◽  
High Accuracy ◽  
Spot Test ◽  
Slow Growing

Protocol for plaque assays - either a full plate plaque assay that are more laborious but with high accuracy, or a spot test plaque assay that are for higher throughput but with less accuracy.

scholarly journals Poultry Salmonella Specific Bacteriophage Isolation and Characterization

2014 ◽  
Vol 12 (2) ◽  
pp. 107-114 ◽  
Author(s):  
MT Rahaman ◽  
M Rahman ◽  
MB Rahman ◽  
MFR Khan ◽  
ML Hossen ◽  
...  
Keyword(s):  
Restriction Site ◽  
Plaque Assay ◽  
Spot Test ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Salmonella Gallinarum ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Phage Dna ◽  
Salmonella Pullorum

Bacteriophage named as SAL-PG (Salmonella bacteriophage), specific to Salmonella pullorum and Salmonella gallinarum was isolated by an enrichment method directly from waste water near to poultry farm. For the isolation of phage 4.5 ml water sample, 0.5 ml of 10X Nutrient Broth and 0.5 ml of log phage bacteria were mixed well and incubated at 37° C for overnight followed by centrifugation at 10,000 rpm for 10 minutes. The presence of bacteriophage was observed by spot test over the bacterial lawn of and plaque assay. The host range of the isolated bacteriophage was determined by spot test using 12 different bacterial isolates. The phages were found to infect Salmonella pullorum, Salmonella gallinarum and Salmonella typhimurium and produced clear plaque on these Salmonella serovars. The bacteriophage was able to survive in wide range of pH between 2 to 9 and resistant at 60° C for 1 hour. More than 50% phages were readily adsorbed to the host bacteria. The restriction enzyme analysis showed that the phage DNA possess restriction site for Taq?I, HindIII and BstYI; but no restriction site for XhoI and BstEII as these two enzymes failed to digest the phage DNA. The characterizations of the bacteriophage would be helpful in establishing a basis for adopting the application of the most effective bacteriophage treatment to control these poultry bacteria.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21264 Bangl. J. Vet. Med. (2014). 12 (2): 107-114 

A New 1,000 kV Electron Microscope

1967 ◽  
Vol 25 ◽  
pp. 260-261
Author(s):  
M. Nishigaki ◽  
S. Katagiri ◽  
H. Kimura ◽  
B. Tadano
Keyword(s):  
Electron Microscope ◽  
High Voltage ◽  
Basic Research ◽  
Chromatic Aberration ◽  
High Accuracy ◽  
Biological Specimen ◽  
Voltage Electron ◽  
High Voltage Electron Microscope ◽  
High Voltage Electron

The high voltage electron microscope has many advantageous features in comparison with the ordinary electron microscope. They are a higher penetrating efficiency of the electron, low chromatic aberration, high accuracy of the selected area diffraction and so on. Thus, the high voltage electron microscope becomes an indispensable instrument for the metallurgical, polymer and biological specimen studies. The application of the instrument involves today not only basic research but routine survey in the various fields. Particularly for the latter purpose, the performance, maintenance and reliability of the microscope should be same as those of commercial ones. The authors completed a 500 kV electron microscope in 1964 and a 1,000 kV one in 1966 taking these points into consideration. The construction of our 1,000 kV electron microscope is described below.

A specific spot test for iron

1960 ◽  
Vol 23 ◽  
pp. 225-226 ◽  
Author(s):  
K LALMALLIK ◽  
B SEN
Keyword(s):  
Spot Test

Application Of BIM-Technology In The Restoration Of Buildings

2020 ◽  
pp. 42-48
Keyword(s):  
Chinese Communist Party ◽  
Laser Scanning ◽  
High Accuracy ◽  
Information Environment ◽  
Information Modeling ◽  
Structural Scheme ◽  
Traditional Design ◽  
Information Interaction ◽  
Heritage Buildings ◽  
Effective Organization

The paper describes the main trends in the development of BIM technologies in the field of restoration and reconstruction of historical and cultural heritage buildings. The practical part of the paper presents the experience in using information modeling technologies when restoring the building, where the VI Congress of the Chinese Communist Party in Moscow took place. The use of laser scanning technologies made it possible to reproduce with high accuracy in the information model the original appearance of the building using Autodesk RevitR software. It is shown, how the use of information modeling technologies affects the duration of restoration process, taking into account the calculation of the structural scheme and bearing structures of the building, ensuring the identity of the decoration and the effective organization of electromechanical installation. Operating in a single BIM information environment makes it possible to continuously obtain reliable information on the project, which provides more effective information interaction and communication of participants compared to using traditional design methods.

Sign in / Sign up

Export Citation Format

Share Document