scholarly journals The estimation of relative DNA content in the genus Spiraea L., section Calospira C. Koch

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Vera A. Kostikova ◽  
◽  
Mariya S. Voronkova ◽  
Evgeny V. Banaev ◽  
Tatiana A. Poliakova ◽  
...  
Keyword(s):  
Dna Content ◽  
Relative Dna Content

scholarly journals Relationships among Morphological Characters, Isozymes Polymorphism and DNA Variability – the Impact on Lactuca Germplasm Taxonomy

2011 ◽  
Vol 39 (No. 2) ◽  
pp. 59-67 ◽  
Author(s):  
I. Doležalová ◽  
A. Lebeda ◽  
M. Dziechciarková ◽  
E. Křístková ◽  
D. Astley ◽  
...  
Keyword(s):  
Dna Content ◽  
Fragment Length Polymorphism ◽  
Morphological Variability ◽  
Morphological Characters ◽  
Aflp Analysis ◽  
Dapi Staining ◽  
Lactuca Species ◽  
Relative Dna Content ◽  
The Impact

Fifty one accessions of nineteen Lactuca species, the hybrid L. serriola × L. sativa and the related species Mycelis muralis were evaluated for morphological variability, esterase (EST) polymorphism, Amplified Fragment Length Polymorphism (AFLP) and relative DNA content. Sixteen Lactuca accessions were classified taxonomically on the basis of morphology, isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded allowing 82% of accessions to be distinguished. The relative DNA content, measured using flow-cytometry (DAPI staining), ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. The results from AFLP analysis and the relative DNA content measurement corresponded well with recent taxonomic classification of the genus Lactuca.  

Flow Cytometrically Determined DNA Content of Breast Carcinoma and Benign Lesions: Correlations with Histopathological Parameters

1986 ◽  
Vol 72 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Raffaella Uccelli ◽  
Alberto Calugi ◽  
Donato Forte ◽  
Francesco Mauro ◽  
Paolo Polonio-Balbi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cell Subpopulation ◽  
Aneuploid Cell ◽  
Breast Carcinomas ◽  
Benign Lesions ◽  
Dna Index ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Relative Dna Content

The relative DNA content of cellular samples from 54 patients affected by breast carcinomas and 20 affected by benign breast lesions (including 11 fibroadenomas) was measured by flow cytometry. All normal tissue samples and 17/20 (85%) specimens from benign lesions exhibited a cytometrically diploid DNA distribution, 3/20 (15%) benign lesions an abnormal DNA content, and 35/54 (65%) carcinomas at least one aneuploid cell subpopulation. Furthermore, 9/54 (17%) tumors were characterized by the presence of more than one aneuploid cell subpopulation. The results also indicate that flow cytometry can be used to recognize lymph nodes infiltrated by aneuploid cells. Statistically significant correlations were evidenced between the occurrence of aneuploidy or the ploidy level measured as DNA index and the nodal infiltration status. The percentage of S cells can also be extracted from DNA content distribution histograms. Statistically significant differences (p < 0.01) were also observed for the percentage of S cells between normal tissues (6.2±3.2 SD) and benign lesions (11.1±6.6 SD), normal tissues (6.2 ± 3.2 SD) and aneuploid tumors (19.7 ± 10.3 SD), benign lesions (11.1 ± 6.6 SD) and aneuploid tumors (19.7 ± 10.3 SD), and diploid (7.9 ± 4.0 SD) and aneuploid tumors (19.7 ± 10.3 SD).

scholarly journals УРОВНИ ПЛОИДНОСТИ И ОТНОСИТЕЛЬНОГО СОДЕРЖАНИЯ ДНК В КУЛЬТУРЕ КЛЕТОК И ТКАНЕЙ РАСТЕНИЙ IN VITRO

2016 ◽  
Vol 6 (3) ◽  
pp. 33-38
Author(s):  
M. V. Skaptsov ◽  
M. A. Krasnoborodkina ◽  
M. G. Kutsev ◽  
S. V. Smirnov ◽  
A. I. Shmakov ◽  
...  
Keyword(s):  
Genome Size ◽  
Ploidy Level ◽  
Dna Content ◽  
Nuclear Dna Content ◽  
Callus Cultures ◽  
Plant Genome ◽  
Control Group ◽  
Ms Medium ◽  
Relative Dna Content

<p>We presented results of variations in the ploidy level and the genome size of the <em>R. acetosa</em> regenerants. These regenerants was obtained by indirect and direct morphogenesis in in vitro culture. Explants were prepared from seedlings on the three-leaf stage of plant development. More than 100 explants were used to stimulate the indirect and direct morphogenesis. Mesophilic explants were cultured on the MS nutrient medium containing auxin to callus proliferation (2 mg/L NAA, 1 mg/L BA). Cultivation of the callus was maintained for 4 weeks followed by an indirect morphogenes. Indirect morphogenesis stimulated on the MS medium with cytokinin and gibberellic acid predominance (0.5 mg/L BA, 0.2 mg/L GA3). Direct stimulate morphogenesis from the apical meristem of seedlings on nutrient media with a predominance of cytokinins (1 mg/L BA, 0.25 mg/L NAA). Rhizogenesis have stimulated by transferring of the regenerants to the ½MS medium supplemented with 0.2 mg/L of NAA. Research of a ploidy level and genome size was performed by flow cytometry used propidium iodide staining with <em>Vicia faba</em> cv “Innovec” (2C=26.90 pg) as internal DNA standard. We calculated the relative DNA content (2C) for <em>R. acetosa</em> equal to 6,98 pg. Cytogenetical analisis showed that the maximum genome size variation recorded for regenerants obtained through the indirect morphogenesis. Variations in the genome size of the regenerants obtained by direct morphogenesis deviates from the control group to 0.30 pg (2С=7.28 pg) and after indirect morphogenesis to 1.04 pg (2С=8.2 pg). Cytogenetical analysis of the regenerated plants showed the presence of different somatic chromosome numbers ranging from 2n = 14 to 2n = 28. The relative DNA content of tetraploid forms was 11.87 pg. In our study was shown, that the most effective method of plant conservation in the <em>in vitro</em> culture is a direct morphogenesis. Analysis of the relative nuclear DNA content and chromosome counts of regenerants obtained by indirect morphogenesis from the callus cultures showed significant variations in the DNA content, as well as the appearance of polyploid forms. Therefore, long-term cultivation of callus cultures increases the probability of genomic aberrations, which reduces the stability of the plant genome.</p>

The Relationship Between Chromosome Volume and Dna Content in Unsquashed Metaphase Cells of Barley, Hordeum Vulgare Cv. Tuleen 346

1982 ◽  
Vol 56 (1) ◽  
pp. 101-111
Author(s):  
M. D. BENNETT ◽  
J. B. SMITH ◽  
J. P. WARD ◽  
R. A. FINCH
Keyword(s):  
Hordeum Vulgare ◽  
Dna Content ◽  
Root Tip ◽  
Hordeum Vulgare L ◽  
Reciprocal Translocations ◽  
Morphological Criteria ◽  
Relative Dna Content ◽  
Sole Criterion ◽  
Mother Cells ◽  
Diploid Cells

The present work used haploid and diploid cells of barley, Hordeum vulgare L. cv. Tuleen 346 (2n = 2x = 14), which has three reciprocal translocations. All seven chromosomes of the haploid set are distinguishable using morphological criteria in Feulgen-stained root-tip squashes seen in the light microscope, as are five of the bivalents at diakinesis. The relative DNA content per bivalent was estimated in pollen mother cells at diakinesis. The results showed that all seven chromosomes or bivalents of Tuleen 346 can be identified using relative DNA content as sole criterion. The absolute and relative volumes of the seven chromosomes were estimated from electron micrographs of serial sections of unsquashed root-tip cells of a haploid. The results show that, using relative chromosome volume as sole criterion, it is highly probable that all seven chromosomes in single unsquashed cells of Tuleen 346 can be correctly identified. Consequently, teats for various non-random spatial arrangements of chromosomes in unsquashed cells of Tuleen 346 using this character to identify the chromosomes should be feasible. There was a very highly significant positive relationship (r&gt;0.99) between relative chromosome volume and mean relative DNA content per chromosome for each cell examined at metaphase of mitosis or meiosis. Thus, some mechanism ensures that the degree of condensation of all seven chromosomes within a cell is usually very similar in Tuleen 346, despite its grossly abnormal karyotype.

Genome size of man and animals relative to the plant Allium cepa

1983 ◽  
Vol 25 (6) ◽  
pp. 554-560 ◽  
Author(s):  
J. Greilhuber ◽  
M. Volleth ◽  
J. Loidl
Keyword(s):  
Cell Line ◽  
Genome Size ◽  
Allium Cepa ◽  
Dna Content ◽  
Animal Species ◽  
Chinese Hamster ◽  
Cho Cell ◽  
Green Toad ◽  
Relative Dna Content ◽  
C Value

A direct Feulgen-cytophotometric comparison of the genomic DNA content (C value) was performed between the liliaceous plant species Allium cepa and a number of animal species to reassess the genome size ratios between plants and animals. These appeared unduely ambiguous as a consequence of divergent picogram estimates in several animal reference species. Taking 1C = 16.75 pg for Allium cepa, the estimates were (1C value in picograms): man, 3.11; Indian muntjak CCL 157 cell line, 2.68; domestic pig, 2.79; Chinese hamster, 2.66; CHO cell line, 2.73; laboratory rat, 2.65; mouse, 3.04; rat kangaroo Pt-K2 cell line, 4.21; fowl, 1.16; and the green toad, 4.30. These values are consistent with a number of independent absolute and relative DNA content determinations reported for animals, and therefore define a coherent set of animal and plant reference values for genome size determinations.

Sign in / Sign up

Export Citation Format

Share Document