Immunohistochemical characteristics of bladder cancer in patients with virus-positive tumors

Background. Viral infection is a major factor in virus-associated carcinogenesis.Objective: to evaluate the expression of growth factors and markers of apoptosis, proliferative activity, and angiogenesis in patients with viral DNA-positive bladder cancer.Materials and methods. The study included 100 bladder cancer patients (72 males and 28 females) aged between 38 and 90 years (mean age 65 ± 10). Tumor tissue samples were tested by polymerase chain reaction to detect DNA of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2 respectively), high-risk human papillomavirus (HPV), cytomegalovirus (CMV), and Epstein—Barr virus (EBV). Immunohistochemical analysis was performed in 32 patients and included the following markers: proliferation marker Ki-67, p63, apoptosis regulator Bcl-2, p53, angiogenesis marker CD31, adhesion protein CD44, and epidermal growth factor receptor (EGFR).Results. Viral DNA in tumor tissue was detected in 34 patients; of them, 50 % had poorly-differentiated tumors. Twenty-seven patients were found to have EBV DNA in their tumor tissue; 6 patients had CMV DNA; 5 patients had high-risk HPV DNA (types 16, 39, 45, 52, and 59); 1 patient had HSV1 and HSV2 DNA. Four out of 34 participants had mixed infections (1 case of HPV 59 + EBV; 2 cases of EBV + CMV; 1 case of CMV + EBV + HPV 31 and 52). We observed a strong correlation between the presence of EBV DNA and levels of CD31 and Ki-67 expression as well as between high-risk HPV DNA and Bcl-2 expression. High levels of antibodies against EBV capsid antigen were associated with EGFR and Ki-67 expression, whereas the level of antibodies against EBV nuclear antigen correlated with CD44 expression. We evaluated specific characteristics of expression of the markers analyzed depending on the tumor stage, grade of anaplasia, and recurrence. We also assessed morphological characteristics of changes in lymphocytic and plasma cell infiltrates.Conclusion. We found a correlation between the presence of viral DNA in bladder cancer tissue and markers of proliferative activity, angiogenesis, and apoptosis. Viral infection is likely to increase proliferative activity and suppression of apoptosis, which may cause tumor progression. Further studies are needed to assess this correlation.

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p16/Ki-67 Immunocytochemistry in Gynecological Cytology: Limitations in Practice

Acta Cytologica ◽

10.1159/000475979 ◽

2017 ◽

Vol 61 (3) ◽

pp. 230-236 ◽

Cited By ~ 2

Author(s):

Peter Ziemke

Keyword(s):

Risk Assessment ◽

High Risk ◽

Positive Result ◽

Dna Testing ◽

Ki 67 ◽

Hpv Dna ◽

Hpv Dna Testing ◽

Immunochemical Detection ◽

High Risk Hpv

Objective: Immunochemical detection of the protein p16INK4a in cervical cytology is used in combination with Ki-67. Cells positive for both proteins are certain to have been transformed by high-risk HPV. p16/Ki-67 immunocytochemistry provides a significant improvement in specificity over cytology and HPV DNA testing. However, p16/Ki-67 immunocytochemistry also has its limitations. Study Design: The research is based on the follow-up of 1,131 patients for whom p16/Ki-67 immunocytochemistry was performed with cytology. Dependencies on the age of patients with LSIL, number of p16/Ki-67-positive cells, and different results during repeated examinations were analyzed. Results: In LSIL, positive p16/Ki-67 is less specific for ≥CIN2/HSIL for patients younger than 30 years compared to patients aged 30 years or older (61.1 vs. 75.7%, p < 0.013). Using a score of 10 p16/Ki-67-marked cells as a positive result instead of 1 led to significantly higher specificity (89.0 vs. 70.2%, p < 0.001). This modified threshold offers better risk assessment in LSIL. In repeated immunocytochemical investigations, 28.4% of the results deviated from the first examination. Conclusion: The abovementioned discrepancies can be interpreted as hints about the molecular biological causes of suboptimal performance of p16/Ki-67. An efficient and reliable application of p16/Ki-67 immunocytochemistry requires knowledge of its methodological limitations.

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Comparison of Human Papillomavirus (HPV) detection in urine and cervical samples using high-risk HPV DNA testing in Northern Thailand

10.26226/morressier.578f37fad462b8028d88f8f5 ◽

2016 ◽

Author(s):

Surapan Khunamornpong

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Dna Testing ◽

Northern Thailand ◽

Hpv Dna ◽

Hpv Dna Testing ◽

High Risk Hpv

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Comparison of Abbott RealTime High Risk HPV and Hybrid Capture 2 for the detection of high-risk HPV DNA in a referral population setting

Journal of Clinical Virology ◽

10.1016/j.jcv.2011.10.016 ◽

2012 ◽

Vol 53 (2) ◽

pp. 121-124 ◽

Cited By ~ 10

Author(s):

Simona Venturoli ◽

Elisa Leo ◽

Martina Nocera ◽

Daniela Barbieri ◽

Monica Cricca ◽

...

Keyword(s):

High Risk ◽

Hybrid Capture ◽

Hpv Dna ◽

Hybrid Capture 2 ◽

High Risk Hpv

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p16 expression in cutaneous squamous cell carcinoma of the head and neck is not associated with integration of high risk HPV DNA or prognosis

Pathology ◽

10.1016/j.pathol.2017.04.002 ◽

2017 ◽

Vol 49 (5) ◽

pp. 494-498 ◽

Cited By ~ 16

Author(s):

Laveniya Satgunaseelan ◽

Noel Chia ◽

Hyerim Suh ◽

Sohaib Virk ◽

Bruce Ashford ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

High Risk ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma ◽

Hpv Dna ◽

High Risk Hpv ◽

P16 Expression

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Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

BioFactors ◽

10.1002/biof.1465 ◽

2018 ◽

Vol 45 (2) ◽

pp. 101-117 ◽

Cited By ~ 10

Author(s):

Pegah Mahmoodi ◽

Mona Fani ◽

Majid Rezayi ◽

Amir Avan ◽

Zahra Pasdar ◽

...

Keyword(s):

Systematic Review ◽

Cervical Cancer ◽

High Risk ◽

Early Detection ◽

Hpv Dna ◽

High Risk Hpv

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Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples

International Journal of Gynecological Cancer ◽

10.1111/igc.0b013e31819bcd0a ◽

2009 ◽

Vol 19 (2) ◽

pp. 266-272 ◽

Cited By ~ 16

Author(s):

Jae Kwan Lee ◽

Mi Kyung Kim ◽

Seung Hun Song ◽

Jin Hwa Hong ◽

Kyung Jin Min ◽

...

Keyword(s):

High Risk ◽

Linear Array ◽

Intraepithelial Neoplasia ◽

Dna Chip ◽

Substantial Agreement ◽

Cervical Lesions ◽

Cycle Sequencing ◽

Hpv Dna ◽

Hpv Dna Tests ◽

High Risk Hpv

Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%;P< 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%;P< 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κcoefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κcoefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with aκvalue of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%,P< 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections.

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Infección por HPV de alto riesgo y lesiones intraepiteliales anales en hombres HIV positivos que tienen sexo con hombres

Actualizaciones en Sida e Infectología ◽

10.52226/revista.v26i97.29 ◽

2018 ◽

Author(s):

Valeria Fink ◽

Laura Svidler López ◽

Fernanda González ◽

Mariana Tejo ◽

Gisela Presencia ◽

...

Keyword(s):

High Risk ◽

Hpv 16 ◽

Hpv Dna ◽

High Risk Hpv

Introducción: El cáncer anal, asociado a la infección con virus de papiloma humano de alto riesgo (HPV-AR), es muy frecuente en hombres que tienen sexo con hombres (HSH) HIV+. Objetivo: Evaluar frecuencia de infección por HPV-AR, genotipos y lesiones asociadas, y factores asociados. Materiales y métodos: Estudio en HSH HIV+ (septiembre 2012-marzo 2014, Hospital Fernández). Se recogió información demográfica, de HIV, HPV y prácticas sexuales. Se realizó citología anal, detección de HPV-AR (HC2 High- Risk HPV DNA, Digene®) y genotipificación en las muestras HPV-AR+ (Inno Lipa®, Fujirebio). Los pacientes firmaron consentimiento informado. Se indicó tratamiento según resultados. Resultados: Completaron el estudio 57 pacientes. Mediana de edad: 40 años (rango intercuartil [RIC]: 29-45); de CD4: 444 cels/mm3 (RIC: 345-568); 77% recibían tratamiento antirretroviral, 68% con carga viral no detectable. Citologías: negativas (24%); lesión intraepitelial de bajo grado (54%); lesión intraepitelial de alto grado (20%); ASCUS (2%). El 80% fue HPV-AR+. Los pacientes con diagnóstico de HPV-AR (p=0,006) y de lesión intraepitelial tuvieron CD4 <500 cels/ mm3 con más frecuencia (p=0,030). Los pacientes con HPV-AR tuvieron mayor frecuencia de carga viral detectable (p=0,020, prueba de Fisher). El porcentaje de pacientes con uso consistente de preservativo fue mayor entre los pacientes sin lesión citológica (p=0,026). Genotipos de alto riesgo más frecuentes: HPV-16 (51%), HPV-31 (44%) y HPV-51 (40%); de bajo riesgo: HPV-6 (47%) y HPV-44 (35%). Conclusiones: Se encontró elevada frecuencia de lesión citológica (76%) y de HPV-AR (80%). Es necesario establecer estrategias de prevención en esta población incluyendo tamizaje, vacunación y promoción de sexo seguro.

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