Changes of exosomal matrix metalloproteinases level in colorectal cancer associated with sex and metabolic disturbance

The objective is to evaluate the contents of matrix metalloproteinases (MMP) MMP9, MMP2, as well as their inducer EMMPRIN in circulating exosomes of patients with colorectal cancer in relation with clinical and morphological parameters, as well as with the presence of metabolic syndrome to search for promising exosomal markers associated with invasion, metastasis and metabolic disorders.Materials and methods. The study included 40 patients with colorectal cancer (T2–4N0–2M0–1) and 10 control patients. Exosomes of blood plasma were isolated by ultrafiltration with ultracentrifugation. The level of MMP9, MMP2 and their inducer EMMPRIN in exosomes was evaluated by flow cytometry.Results and conclusion. The level of MMP9‑positive exosomes was significantly higher in patients with colorectal cancer compared with patients with colorectal polyps. The proportion of MMP9‑negative and triple positive MMP9 + / MMP2+ / EMMPRIN+ exosomes, on the contrary, was higher in patients with polyps compared with patients with colorectal cancer. Mixed subpopulation of MMP9+ / MMP2– / EMMPRIN-exosomes prevailed both in patients with colorectal cancer and in control patients. There were no significant differences in the subpopulations of MMP and EMMPRIN in the exosomes of colorectal cancer patients depending on the age, stage, grade and localization. Gender differences in the occurrence of a triple-positive exosome subpopulation in colorectal cancer patients have been revealed. No relationship was found between the expression of MMP and EMMPRIN in exosomes and the presence of the metabolic syndrome, anthropometric parameters, the level of total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol. However, the relationships between MMP9+ / MMP2– / EMMPRIN–, MMP9+ / MMP2– / EMMPRIN– and the level of triglycerides and glucose in blood serum were revealed. Further studies are needed to study the characteristics of exosomes associated with metabolic disorders and the possibility of their use as diagnostic, prognostic, or predictor biomarkers.

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Association of Metabolic Syndrome and Cardiovascular Risk Factors Among Colorectal Cancer Patients Attending in Tikur Anbessa Specialized Hospital Oncology Units: A Comparative Cross-sectional Study June, 2017 Addis Ababa, Ethiopia

10.21203/rs.3.rs-73476/v1 ◽

2020 ◽

Author(s):

Shimelis Adugna Elemo ◽

Bereket Mihretu Awoke

Keyword(s):

Colorectal Cancer ◽

Risk Factors ◽

Metabolic Syndrome ◽

Cancer Patients ◽

Addis Ababa ◽

Density Lipoprotein ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Colorectal Cancer Patients

Abstract Background: Metabolic syndrome and obesity are risk factors for developing type 2 diabetes mellitus and/or cardiovascular disease, especially stroke. Obesity-associated diseases account for a large portion of public health challenges. Among obesity-related disorders, a direct and independent relationship has been ascertained for colorectal cancer. Objective: The aim of this study was to determine the prevalence of metabolic syndrome and other cardiovascular risk factors, including dyslipidemia, hypertension, dietary and exercise practices, tobacco use and body mass index in individual with colorectal cancer patients attending Tikur Anbessa Hospital Oncology department, Addis Ababa.Methods: A comparative cross-sectional study was conducted from February, 2016 to Jun 2017 on 79 patients attending the Oncology clinic at Tikur Anbessa Hospital. Components of metabolic syndrome, including fasting serum glucose, serum triglyceride, serum high density lipoprotein, blood pressure and waist circumference, was measured in all of the patients. In addition, serum total cholesterol, serum low density lipoprotein was measured with 5010 photometers, and information gathered on smoking and other lifestyle practices, including diet and exercise.Result: Of 79 patients, 23 had metabolic syndrome (MS); 58.2% males; mean-age 48.6 years with range (26 to 78) and 41.8 % Female; mean age 44.6 years with range (20 to 78). Among the MS group, 45.6 % had high blood pressure, 26.6 % had increased waist circumference, 67.1% had serum blood glucose level greater than 100 mg/dL, 68.3 % had low high-density lipoprotein cholesterol level, 57 % had increased triglyceride level, and 3.8 % were obese [body mass index (BMI) >30 kg/m2]. 29 % presented 3 criteria of MS. From total colorectal cancer patients 53.2 % had colon cancer, 46.8 % had rectal cancer and 12.7 % had stage I,40.5 % had stage II (IIa,IIb & IIc), 25.3 % had stage III (a,b,c) and 21.5 % had stage IV disease.CONCLUSION: MS is positively associated with adenomas and colorectal cancer. However, there is not enough information in Ethiopia to justify screening in patients with MS. To our knowledge, no previous study has evaluated this association in Ethiopian patients.

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Diabetes, Obesity, and the Metabolic Syndrome as Prognostic Factors in Stages I to III Colorectal Cancer Patients

Journal of Gastrointestinal Cancer ◽

10.1007/s12029-018-0056-9 ◽

2018 ◽

Vol 50 (2) ◽

pp. 221-229 ◽

Cited By ~ 12

Author(s):

Brianna Croft ◽

Melissa Reed ◽

Caitlyn Patrick ◽

Natalie Kovacevich ◽

Ioannis A. Voutsadakis

Keyword(s):

Colorectal Cancer ◽

Metabolic Syndrome ◽

Prognostic Factors ◽

Cancer Patients ◽

The Metabolic Syndrome ◽

Colorectal Cancer Patients

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Abdominal obesity, glucose intolerance and decreased high-density lipoprotein cholesterol as components of the metabolic syndrome are associated with the development of colorectal cancer

European Journal of Epidemiology ◽

10.1007/s10654-018-0440-6 ◽

2018 ◽

Vol 33 (11) ◽

pp. 1077-1085 ◽

Cited By ~ 12

Author(s):

Yoon Jin Choi ◽

Dong Ho Lee ◽

Kyung-Do Han ◽

Cheol Min Shin ◽

Nayoung Kim

Keyword(s):

Colorectal Cancer ◽

Metabolic Syndrome ◽

High Density Lipoprotein ◽

Glucose Intolerance ◽

Abdominal Obesity ◽

High Density Lipoprotein Cholesterol ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

The Metabolic Syndrome

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Effect of the components of the metabolic syndrome on pulmonary function. The unexpected role of high-density lipoprotein cholesterol

Cirugía y Cirujanos (English Edition) ◽

10.24875/cirue.m18000025 ◽

2019 ◽

Vol 86 (2) ◽

Author(s):

Saúl Huerta-Ramírez ◽

Angélica Paniagua-Pérez ◽

David Castro-Serna ◽

Andrés Ledesma-Velázquez ◽

Alberto Rubio-Guerra ◽

...

Keyword(s):

Metabolic Syndrome ◽

Pulmonary Function ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

The Metabolic Syndrome

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Serum interleukin-23 protects, whereas methotrexate treatment stimulates selected components of the metabolic syndrome in patients with SAPHO syndrome

Archives of Medical Science ◽

10.5114/aoms.2018.76953 ◽

2021 ◽

Vol 17 (1) ◽

pp. 120-126

Author(s):

Hanna Przepiera-Będzak ◽

Katarzyna Fischer ◽

Marek Brzosko

Keyword(s):

Metabolic Syndrome ◽

Growth Factor ◽

Negative Correlation ◽

Density Lipoprotein ◽

Sapho Syndrome ◽

Lipoprotein Cholesterol ◽

Skin Changes ◽

The Metabolic Syndrome ◽

Methotrexate Treatment ◽

The Impact

IntroductionThe aim of the study was to evaluate the impact of disease activity, selected serum cytokines, and therapy on metabolic syndrome (MetS) components in patients with synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome.Material and methodsWe studied 46 SAPHO patients (40 women, 6 men). We recorded age, sex, disease duration, arthritis localization, type of skin changes, bone scintigraphy results, comorbidities, BASDAI, VAS, and treatment. We measured erythrocyte sedimentation rate, C-reactive protein, lipid profile, serum IL-6, IL-18, IL-23, endothelin-1, vascular endothelial growth factor, and epidermal growth factor (EGF).Results97.8% of patients had sternoclavicular joint arthritis, 91.3% of patients palmoplantar pustulosis. In 65.2% of SAPHO patients skin changes and arthritis started simultaneously. Apart from non-steroidal anti-inflammatory drugs, patients were treated with methotrexate (41.3%), sulfasalazine (41.3%), and antibiotics (39.1%). 19.5% of patients met MetS criteria. Serum IL-23 correlated positively with total cholesterol (TC; p = 0.02) and high-density lipoprotein cholesterol (HDL-C) (p = 0.01) in the SAPHO group. There was a negative correlation between HDL-C and BASDAI (p = 0.02). Patients treated with methotrexate had higher triglyceride (p = 0.01) and low-density lipoprotein cholesterol (LDL-C) (p = 0.01) levels. There was a negative correlation between TC and EGF (p = 0.03). Increased prevalence of autoimmune diseases and depression was observed in SAPHO patients.ConclusionsSerum IL-23 protects, whereas methotrexate treatment stimulates selected components of the MetS in patients with SAPHO syndrome.

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Small, Dense Lipoprotein Particles and Reduced Paraoxonase-1 in Patients with the Metabolic Syndrome

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2004-1295 ◽

2005 ◽

Vol 90 (4) ◽

pp. 2264-2269 ◽

Cited By ~ 54

Author(s):

Marie-Claude Blatter Garin ◽

Barbara Kalix ◽

Alfredo Morabia ◽

Richard W. James

Keyword(s):

Coronary Artery Disease ◽

Metabolic Syndrome ◽

Coronary Artery ◽

Coronary Disease ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Paraoxonase 1 ◽

Lipoprotein Particles ◽

The Metabolic Syndrome ◽

Artery Disease

Abstract The presence of the metabolic syndrome (World Health Organization definition) and its association with lipoprotein abnormalities suggestive of greater susceptibility to oxidative stress have been analyzed in patients with angiographically defined coronary artery disease. The odds ratio for the presence of the metabolic syndrome was significantly higher in coronary artery disease-positive patients (P < 0.001). The metabolic syndrome was also associated with more severe coronary disease (P < 0.01). Patients with the metabolic syndrome had significantly decreased low-density lipoprotein-cholesterol/apolipoprotein B and high-density lipoprotein-cholesterol/apolipoprotein AI ratios, indicative of the presence of small, dense lipoprotein particles. The syndrome was also associated with reduced concentrations and activities of the antioxidant enzyme, paraoxonase-1. The metabolic syndrome is characterized by smaller, denser lipoprotein particles that increase their susceptibility to oxidative modifications and diminished serum paraoxonase-1, which is a major determinant of the antioxidant capacity of high-density lipoproteins. These may be contributory factors to the increased presence and severity of coronary disease in such patients.

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