VARIABILITY OF PELARGONIUM PELTATUM CULTIVAR IN LONG-TERM IN VITRO CULTURE OF DISEASE-FREE NUCLEUS STOCKS.

1988 ◽  
pp. 647-654 ◽  
Author(s):  
G. Reuther
Keyword(s):  
In Vitro Culture ◽  
Pelargonium Peltatum ◽  
Disease Free ◽  
Free Nucleus

scholarly journals Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

2021 ◽  
Vol 22 (13) ◽  
pp. 6663
Author(s):  
Maurycy Jankowski ◽  
Mariusz Kaczmarek ◽  
Grzegorz Wąsiatycz ◽  
Claudia Dompe ◽  
Paul Mozdziak ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Vitro Culture ◽  
Osteogenic Differentiation ◽  
Molecular Mechanisms ◽  
Marker Genes ◽  
P Value ◽  
Illumina Platform

Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.

Slush nitrogen vitrification of human ovarian tissue does not alter gene expression and improves follicle health and progression in long-term in vitro culture

2018 ◽  
Vol 110 (7) ◽  
pp. 1356-1366 ◽  
Author(s):  
Vincenza Barbato ◽  
Roberto Gualtieri ◽  
Teresa Capriglione ◽  
Maria Michela Pallotta ◽  
Sabrina Braun ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue

Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1623-1636 ◽  
Author(s):  
Chu-Chih Shih ◽  
Mickey C.-T. Hu ◽  
Jun Hu ◽  
Jeffrey Medeiros ◽  
Stephen J. Forman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Ex Vivo ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Culture System

Abstract We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD34+thy-1+ cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD34+ thy-1+ cells on AC6.21 stroma, a murine bone marrow–derived stromal cell line, caused expansion of cells with CD34+ thy-1+ phenotype. Addition of other cytokines, including interleukin-3 (IL-3), IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, to LIF in the cultures caused a 150-fold expansion of cells retaining the CD34+ thy-1+ phenotype. The ex vivo–expanded CD34+ thy-1+ cells gave rise to multilineage differentiation, including myeloid, T, and B cells, when transplanted into SCID-hu mice. Both murine LIF (cannot bind to human LIF receptor) and human LIF caused expansion of human CD34+ thy-1+ cells in vitro, suggesting action through the murine stroma. Furthermore, another human HSC candidate, CD34+ CD38− cells, shows a similar pattern of proliferative response. This suggests thatex vivo expansion of transplantable human stem cells under this in vitro culture system is a general phenomenon and not just specific for CD34+ thy-1+ cells.

scholarly journals Functional properties of bone marrow-derived MSC-based engineered cartilage are unstable with very long-term in vitro culture

2014 ◽  
Vol 47 (9) ◽  
pp. 2173-2182 ◽  
Author(s):  
Megan J. Farrell ◽  
Matthew B. Fisher ◽  
Alice H. Huang ◽  
John I. Shin ◽  
Kimberly M. Farrell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Vitro Culture ◽  
Functional Properties ◽  
Engineered Cartilage

scholarly journals Ectopic activation of WNT signaling in human embryonal carcinoma cells and its effects in short- and long-term in vitro culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yaser Atlasi ◽  
Rebecca T. van Dorsten ◽  
Andrea Sacchetti ◽  
Rosalie Joosten ◽  
J. Wolter Oosterhuis ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
In Vitro Culture ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Ectopic Activation ◽  
Short And Long Term

Suboptimal in vitro culture conditions: an epigenetic origin of long-term health effects

2007 ◽  
Vol 74 (9) ◽  
pp. 1149-1156 ◽  
Author(s):  
Raúl Fernández-Gonzalez ◽  
Miguel Angel Ramirez ◽  
Ainhoa Bilbao ◽  
Fernando Rodríguez De Fonseca ◽  
Alfonso Gutiérrez-Adán
Keyword(s):  
In Vitro Culture ◽  
Health Effects ◽  
Culture Conditions

Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1623-1636 ◽  
Author(s):  
Chu-Chih Shih ◽  
Mickey C.-T. Hu ◽  
Jun Hu ◽  
Jeffrey Medeiros ◽  
Stephen J. Forman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Ex Vivo ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Culture System

We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD34+thy-1+ cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD34+ thy-1+ cells on AC6.21 stroma, a murine bone marrow–derived stromal cell line, caused expansion of cells with CD34+ thy-1+ phenotype. Addition of other cytokines, including interleukin-3 (IL-3), IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, to LIF in the cultures caused a 150-fold expansion of cells retaining the CD34+ thy-1+ phenotype. The ex vivo–expanded CD34+ thy-1+ cells gave rise to multilineage differentiation, including myeloid, T, and B cells, when transplanted into SCID-hu mice. Both murine LIF (cannot bind to human LIF receptor) and human LIF caused expansion of human CD34+ thy-1+ cells in vitro, suggesting action through the murine stroma. Furthermore, another human HSC candidate, CD34+ CD38− cells, shows a similar pattern of proliferative response. This suggests thatex vivo expansion of transplantable human stem cells under this in vitro culture system is a general phenomenon and not just specific for CD34+ thy-1+ cells.

scholarly journals Improvement of culture conditions for long-term in vitro culture of Plasmodium vivax

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Wanlapa Roobsoong ◽  
Chayada S Tharinjaroen ◽  
Nattawan Rachaphaew ◽  
Porpimon Chobson ◽  
Louis Schofield ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Plasmodium Vivax ◽  
Culture Conditions

scholarly journals Human prothymocytes. Membrane properties, differentiation patterns, glucocorticoid sensitivity, and ultrastructural features.

1980 ◽  
Vol 152 (4) ◽  
pp. 796-807 ◽  
Author(s):  
U Galili ◽  
A Polliack ◽  
E Okon ◽  
R Leizerovitz ◽  
H Gamliel ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Mitotic Activity ◽  
Membrane Properties ◽  
Nuclear Chromatin ◽  
Peanut Agglutinin ◽  
Chromatin Pattern ◽  
Highly Sensitive ◽  
Thymic Cell

Thymic precursor cells (prothymocytes) comprise a large proportion of the fetal thymic cell population, but are less frequently encountered in the postnatal thymus, where they compose &lt; 1% of the entire population. In the present study we attempted to characterize a number of properties of the prothymocytes obtained from human fetal thymic tissues after depletion of the E-rosette thymocyes on a Ficoll-Hypaque gradient. The prothymocytes are larger than the thymocytes and show a different nuclear chromatin pattern. This subset of cells lacks the E-rosetting and natural-attachment capacities and, unlike thymocytes, does not bind the lectin peanut agglutinin. Human prothymocytes are highly sensitive to the in vitro cytolytic effect of hydrocortisone, whereas the thymocytes are resistant. Long-term in vitro culture of prothymocytes resulted in the expression of thymocyte characteristics together with a burst of mitotic activity. Results of this study indicate that the rate of the prothymocyte proliferation is regulated by the small thymocytes present in the same suspension.

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