CARBON SOURCE REQUIREMENT FOR THE CULTURE OF WHITE YAM (DIOSCOREA ROTUNDATA) EMBRYOS IN VITRO

1994 ◽  
pp. 329-334 ◽  
Author(s):  
C.E.A. Okezie ◽  
S.N.C. Okonkwo ◽  
F.I.O. Nwoke
Author(s):  
Kavitha K ◽  
Asha S ◽  
Hima Bindu T.V.L ◽  
Vidyavathi M

The safety and efficacy of a drug is based on its metabolism or metabolite formed. The metabolism of drugs can be studied by different in vitro models, among which microbial model became popular. In the present study, eight microbes were screened for their ability to metabolize phenobarbital in a manner comparable to humans with a model to develop alternative systems to study human drug metabolism. Among the different microbes screened, a filamentous fungi Rhizopus stolonifer metabolized phenobarbital to its metabolite which is used for further pharmacological and toxicological studies. The transformation of phenobarbital was identified by high- performance liquid chromatography (HPLC). Interestingly, Rhizopus stolonifer sample showed an extra metabolite peak at 3.11min. compared to its controls. The influence of different carbon sources in media used for growth of fungus, on metabolite production was studied, to find its effect in production of metabolite as the carbon source may influence the growth of the cell.


2009 ◽  
Vol 147 (5) ◽  
pp. 589-600 ◽  
Author(s):  
S. MORSE ◽  
N. McNAMARA ◽  
M. ACHOLO

SUMMARYYam minisett technique (YMT) has been promoted throughout West Africa since the 1980s as a sustainable means of producing clean yam planting material, but adoption of the technique is often reported as being patchy at best. While there has been much research on the factors that influence adoption of the technique, there have been no attempts to assess its economic viability under ‘farmer-managed’ as distinct from ‘on station’ conditions. The present paper describes the results of farmer-managed trials employing the YMT (white yam: Dioscorea rotundata) at two villages in Igalaland, Kogi State, Nigeria. One of the villages (Edeke) is on the banks of the River Niger and represents a specialist yam environment, whereas the other village (Ekwuloko) is inland, where farmers employ a more general cropping system. Four farmers were selected in each of the two villages and asked to plant a trial comprising two varieties of yam, their popular local variety as well as another variety grown in other parts of Igalaland, and to treat yam setts (80–100 g) with either woodash or insecticide/nematicide+fungicide mix (chemical treatment). Results suggest that while chemical sett treatment increased yield and hence gross margin compared with woodash, if household labour is costed then YMT is not economically viable. However, the specialist yam growers of Edeke were far more positive about the use of YMT as they tended to keep the yam seed tubers for planting rather than sell them. Thus, great care needs to be taken with planning adoption surveys on the assumption that all farmers should adopt a technology.


2021 ◽  
Author(s):  
Samed Güner ◽  
Vanessa Wegat ◽  
André Pick ◽  
Volker Sieber

Realizing a sustainable future requires intensifying the waste stream conversion, such as converting the greenhouse gas carbon dioxide into value-added products. In this paper, we focus on utilizing formaldehyde as...


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