EFFICACY OF DIMETHYL DISULFIDE (DMDS) IN THE CONTROL OF THE ROOT-KNOT NEMATODE MELOIDOGYNE INCOGNITA AND THE CYST NEMATODE HETERODERA CAROTAE ON CARROT IN FIELD CONDITION IN ITALY

2014 ◽  
pp. 405-410 ◽  
Author(s):  
G. Curto ◽  
C. Dongiovanni ◽  
N. Sasanelli ◽  
A. Santori ◽  
A. Myrta
Keyword(s):  
Field Condition ◽  
Meloidogyne Incognita ◽  
Dimethyl Disulfide ◽  
Cyst Nematode ◽  
Root Knot Nematode

scholarly journals Dimethyl Disulfide- A Potential Biopesticide Against Root-Knot Nematode of Tomato (Lycopersicon Esculentum L.)

2012 ◽  
Vol 36 (4) ◽  
pp. 685-695 ◽  
Author(s):  
MI Faruk ◽  
ML Rahman ◽  
MMM Mustafa ◽  
IR J Coosemans
Keyword(s):  
Lycopersicon Esculentum ◽  
Meloidogyne Incognita ◽  
Tomato Plant ◽  
Vegetative Growth ◽  
Dimethyl Disulfide ◽  
Soil Nematode ◽  
Root Knot Nematode ◽  
Tomato Seedlings ◽  
Dry Conditions ◽  
Pot Culture

Dimethyl disulfide (DMDS), the natural biopesticide extracted from Allium spp., was evaluated against root-knot nematode (Meloidogyne incognita) of tomato (Lycopersicon esculentum L.) in greenhouse pot culture. All concentrations of DMDS viz. 30 ml, 60 ml, and 80 ml and Aldicarb @ 2g per square meter of soil were effective against root-knot disease under both wet and dry conditions of soil. Nematode incidence was reduced drastically by higher dose of DMDS and Aldicarb @ 2g but did not accelerate vegetative growth of tomato plant especially when tomato seedlings were transplanted immediately after soil treatment. Low concentration of DMDS (30 ml per square meter of soil) was found appropriate for controlling root-knot nematode of tomato, accelerating saprophytic nematode population in soil and also enhancing vegetative growth of tomato plant under dry condition of soil.   DOI: http://dx.doi.org/10.3329/bjar.v36i4.11759   Bangladesh J. Agril. Res. 36(4): 685-695, December 2011  

Water Uptake and Hatching in the Potato Cyst Nematode, Heterodera Rostochiensis, and the Root Knot Nematode, Meloidogyne Incognita

1974 ◽  
Vol 61 (3) ◽  
pp. 773-779
Author(s):  
C. ELLENBY
Keyword(s):  
Water Content ◽  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Cyst Nematode ◽  
Interference Microscopy ◽  
Root Knot Nematode ◽  
Second Stage ◽  
History Of ◽  
Heterodera Rostochiensis ◽  
Egg Shell

1. The second-stage larva of the plant parasitic nematode Heterodera rostochiensis shows very little movement while confined to the egg; on the other hand, the larva of the closely related Meloidogyne incognita is very active 2. Using interference microscopy, it is shown that the water content of the Heterodera larva increases immediately it is liberated from the egg while that of the Meloidogyne larva remains constant. 3. It is suggested that the Heterodera larva is physically constricted by the egg shell and that this restricts both its movement and its water content. 4. Measurement of larval and egg volume in both forms supports this hypothesis. The Meloidogyne larva has about 30% of free space inside the egg shell while the Heterodera larva fits it completely. 5. It is suggested that these differences can be correlated with differences in the distribution and life-history of the two forms.

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Nematology ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Susan Meyer ◽  
Robin Huettel ◽  
Xing Zhong Liu ◽  
Richard Humber ◽  
Jean Juba ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Fungal Biomass ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Fungal Culture ◽  
Root Knot Nematode ◽  
Egg Hatch ◽  
Potato Dextrose Broth ◽  
Fungal Isolates

Abstract Fungi were isolated from soybean cyst nematode (SCN, Heterodera glycines) eggs collected in China, and 253 fungal isolates were assayed for production of compounds active against SCN and root-knot nematode (RKN, Meloidogyne incognita). Fungal isolates were grown for 3 and 7 days in potato dextrose broth (PDB), the culture broths were sterile-filtered to remove fungal biomass, and the filtrates were placed into 24-well plates to test for effects on egg hatch and juvenile motility. Meloidogyne incognita egg hatch ranged from 2 to 121% of hatch in PDB controls and H. glycines hatch from 15 to 224%. Activities of filtrates harvested after 3 and 7 days were significantly correlated. Only four isolates produced filtrates that significantly inhibited juvenile motility of SCN, RKN or both nematodes. This study identified fungal isolates capable of producing compounds active against these nematodes, and demonstrated that there was a low correlation in activity against SCN and RKN. The active fungal isolates are candidates for studies on identification of potential nematicides.

scholarly journals Plant Volatiles Reduce the Viability of the Root-Knot Nematode Meloidogyne incognita Either Directly or When Retained in Water

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2170-2179 ◽  
Author(s):  
Julio Carlos P. Silva ◽  
Vicente P. Campos ◽  
Aline F. Barros ◽  
Marcio P. Pedroso ◽  
Willian C. Terra ◽  
...  
Keyword(s):  
Plant Species ◽  
Meloidogyne Incognita ◽  
Sunflower Seed ◽  
Dimethyl Sulfide ◽  
Dimethyl Disulfide ◽  
Parasitic Nematodes ◽  
Root Knot Nematode ◽  
Tomato Roots ◽  
Chemical Groups

Volatile organic compounds (VOC) produced by green residues for the management of plant-parasitic nematodes are poorly studied for oilseed plants and some Brassica spp. To investigate the activity of VOC in vitro and as biofumigants, dry and aqueous macerates of broccoli (Brassica oleracea var. italica) shoots and sunflower (Helianthus annuus) seed were used against the root-knot nematode Meloidogyne incognita. VOC produced by sunflower seed caused higher mortality of M. incognita second-stage juveniles (J2) than VOC produced by broccoli shoots but both plant species were equally effective in decreasing the infectivity and reproduction of this nematode. The number of galls and eggs produced by the nematode in tomato roots was reduced by 89 and 95%, respectively, on average, at the highest concentrations of broccoli and sunflower seed macerates tested as biofumigants. When nematodes were placed in water exposed to broccoli VOC, J2 immobility increased and the number of galls and eggs produced by the nematode in tomato roots decreased 80 and 96%, respectively. Water exposed to sunflower seed VOC had no effect on the viability of the nematode. Gas chromatography was used to identify five and six chemical groups in broccoli and in sunflower seed macerates, respectively, but only alcohols, sulfurated VOC, and terpenes were detected in the water exposed to these plant macerates. Sulfurated VOC from the water exposed to broccoli macerates were found to be involved in its activity against M. incognita. The purified VOC dimethyl disulfide (DMDS) and 3-pentanol were tested directly against J2 and showed a lethal concentration of 176 and 918 µg/ml (ppm), respectively, whereas dimethyl sulfide had no effect against M. incognita. Furthermore, DMDS and 3-pentanol retained in water killed J2 and reduced gall formation and the number of eggs of M. incognita on tomato roots. Both these plant species produced toxic VOC to M. incognita, whereas only VOC retained in water exposed to broccoli had activity against M. incognita.

Bacterial volatile-mediated suppression of root-knot nematode (Meloidogyne incognita)

Plant Disease ◽  
2021 ◽  
Author(s):  
Ting Yang ◽  
Yi Xin ◽  
Tongyao Liu ◽  
Zhengfeng Li ◽  
Xingzhong Liu ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Dimethyl Disulfide ◽  
Gas Chromatography Mass Spectrometry ◽  
Economic Losses ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Juvenile Mortality ◽  
Rhizosphere Microorganisms ◽  
Important Approach ◽  
Meloidogyne Spp

Root-knot nematodes (Meloidogyne spp.) are obligate plant parasites that cause severe economic losses to agricultural crops worldwide. Due to serious health and environmental concerns related to the use of chemical nematicides, the development of efficient alternatives is of great importance. Biological control through exploiting the potential of rhizosphere microorganisms is currently accepted as an important approach for pest management in sustainable agriculture. In our research, during screening of rhizosphere bacteria against the root-knot nematodes Meloidogyne incognita, Ochrobactrum pseudogrignonense strain NC1 from the rhizosphere of healthy tomatoes showed strong nematode inhibition. A volatile nematicidal assay showed that the cell-free fermentation filtrate in the first-row wells of 12-well tissue culture plates caused M. incognita juvenile mortality in the second-row wells. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that dimethyl disulfide (DMDS) and benzaldehyde were the main volatile compounds produced by strain NC1. The nematicidal activity of these compounds indicated that the LC50 against the M. incognita juveniles in the second-row wells and the fourth-row wells were 23.4 μmol/mL and 30.7 μmol/mL for DMDS and 4.7 μmol/mL and 15.2 μmol/mL for benzaldehyde, respectively. A greenhouse trial using O. pseudogrignonense strain NC1 provided management efficiencies of root-knot nematodes of 88 to 100% compared with the untreated control. This study demonstrated that nematode-induced root-gall suppression mediated by the bacterial volatiles DMDS and benzaldehyde presents a new opportunity for root-knot nematode management.

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