Protocorms encapsulation of Phalaenopsis hybrids (Orchidaceae) in order to schedule in vitro plantlet production

2020 ◽  
pp. 63-68
Author(s):  
Z. Mahdavi ◽  
Sh. Dianati Daylami ◽  
S. Aliniaeifard
Keyword(s):  
Plantlet Production

scholarly journals In vitro plantlet production of the endangered Pinguicula vulgaris

2012 ◽  
Vol 7 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Tomás Grevenstuk ◽  
Anabela Romano
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Normal Development ◽  
Shoot Proliferation ◽  
Carnivorous Plant ◽  
Habitat Requirements ◽  
Ex Vitro ◽  
Plantlet Production

AbstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

scholarly journals Mikropropagasi planlet tebu menggunakan sistem perendaman sesaat (SPS) Micropropagation of sugarcane plantlets using temporary immersion system (TIS)

2016 ◽  
Vol 81 (1) ◽  
Author(s):  
Hayati MINARSIH ◽  
Imron RIYADI ◽  
. SUMARYONO ◽  
Asmini BUDIANI
Keyword(s):  
Solid Medium ◽  
Temporary Immersion System ◽  
Production Scale ◽  
Temporary Immersion ◽  
Biotechnology Research ◽  
Plantlet Production ◽  
Self Sufficiency ◽  
National Production ◽  
Planting Materials

bstractTo achieve Indonesian sugar self-sufficiency in2014, the national production needs to be escalatedthrough land extensification that requires a largenumbers of cane planting materials. This can be achievedby mass propagation of sugarcane through in vitroculture. Solid medium is commonly used for callusproliferation in sugarcane tissue culture. However, solidmedium is considered inefficient in terms of plantletproduction level, labour and space. The use of liquidmedium may solve the problem by allowing automationto increase plantlet production scale and uniformity.Temporary immersion system (TIS) is based on a shortperiodic immersion of explants in a liquid medium for aspecific frequency and duration. Research on in vitromass propagation of sugarcane using TIS was conductedat the Indonesian Biotechnology Research Institute forEstate Crops. Callus initiated from immature unfoldedleaves of PSJT 941 and PS 881 was cultured on liquidMS medium in TIS with different frequencies (12 and24 h) and durations (1 and 3 min) of immersion. Eachtreatment was replicated three times. The callus biomassof two elite cane varieties (PSJT 941 and PS 881)cultured in TIS for six weeks was higher (2 – 4 times fold)than that of on solid medium. The PSJT 941 varietyreached the highest calli biomass with immersion forthree min every 24 h. However, PS 881 variety reachedits highest biomass with immersion for one minute every24 h. The propagation of sugarcane using TIS culturewas proven to produce higher calli biomass up to fourfolds and to form more numbers and uniform shootscompared to the solid medium culture. The callus wassuccesfully regenerated to shoots and plantlets.AbstrakUntuk mencapai swasembada gula, perlu dilakukanpeningkatan produksi gula nasional melalui perluasanareal pertanaman tebu sehingga diperlukan bibit dalamjumlah besar. Hal tersebut dapat diatasi antara laindengan perbanyakan tebu melalui kultur in vitro. Peng-gunaan medium padat pada perbanyakan kalus tebumelalui kultur in vitro merupakan teknik yang umumdigunakan saat ini. Akan tetapi penggunaan mediumpadat dianggap kurang efisien dalam hal jumlah planletyang diproduksi, tenaga kerja dan ruang digunakan.Penggunaan medium cair dapat mengatasi kelemahantersebut dengan dimungkinkannya otomatisasi sehinggadapat meningkatkan skala produksi secara massal dankeseragaman planlet. Sistem perendaman sesaat (SPS)merupakan teknik kultur in vitro dalam medium cairmenggunakan bejana bersekat dimana kontak antaraeksplan dan medium terjadi hanya secara sesaat danperiodik. Penelitian perbanyakan massal bibit tebumelalui SPS dilakukan di Balai Penelitian BioteknologiPerkebunan Indonesia. Kalus diinisiasi dari daun meng-gulung varietas PSJT 941 dan PS 881 yang ditumbuhkanpada media MS cair dalam kultur SPS dengan frekuensiyang berbeda (12 dan 24 jam) dan lama perendaman (1dan 3 menit). Setiap perlakuan diulang tiga kali. Bobotbasah (biomassa) kalus dari dua varietas tebu (PSJT 941dan PS 881) yang ditumbuhkan dengan metode SPSsetelah enam minggu menunjukkan pening-katan yanglebih tinggi yaitu antara 2 - 4 kali lipat dibandingkandengan kontrol (media padat). Peningkatan biomassatertinggi pada varietas PSJT 941 diperoleh pada per-lakuan SPS dengan interval perendaman 24 jam dan lamaperendaman tiga menit. Sedangkan pada PS 881,peningkatan tertinggi biomassa diperoleh pada intervalperendaman 24 jam dan lama perendaman satu menit.Perbanyakan dengan metode SPS terbukti dapat mening-katkan biomassa kalus lebih dari empat kali lipat danpembentukan tunas yang lebih seragam dibandingkandengan pada media padat. Kalus yang dihasilkan dapatdiregenerasikan menjadi tunas dan planlet.

scholarly journals In vitro Gynogenic Plant Production in Allium tuncelianum (Kollman) Özhatay, Matthew & Şiraneci

2021 ◽  
Author(s):  
Fevziye Celebi-Toprak ◽  
Zeynep Ergun ◽  
Ali R. Alan
Keyword(s):  
Plant Production ◽  
Abnormal Development ◽  
Naphthalene Acetic Acid ◽  
Flower Buds ◽  
Plantlet Production ◽  
Induction Experiment ◽  
Improvement Programs ◽  
Production Frequency ◽  
Diploid Cells

Abstract Allium tuncelianum (Kollman) Özhatay, Matthew & Şiraneci forms a single-cloved edible white bulb with mild garlic (A. sativum) odour and taste. Its ability to form seeds make it suitable for genetic improvement via classical and modern approaches. A detailed study was carried out to determine the gynogenesis potential of two A. tuncelianum (AT1 and AT2) accessions. Unopened flower buds of A. tuncelianum accessions were cultured in various BDS- and MS-based induction media. A total of 17 (0.09%) gynogenic plantlets were obtained from ~20000 flower buds used in gynogenesis induction experiment. Accessions showed slight differences in their responses to gynogenesis induction cultures. The highest gynogenic plantlet production frequency (0.34 %) in AT1 was achieved flower buds cultured in T12 medium (MS with 100 g/L sucrose, 1 mg/L a-naphthalene acetic acid (NAA) and 8 mg/L isopentenyl adenine (2IP). Flower buds of AT2 showed the highest gynogenic plantlet production response (0.44 %) in T2 medium (BDS with 50 g/L sucrose). Eight of 17 gynogenic plantlets continued to grow and became healthy plants with green leaves and well established roots. Flow cytometric (FCM) analysis of well-developed gynogenic plants showed that two were haploid (25 %), four were diploid (50 %), and two were mixoploid (25 %) for haploid and diploid cells. Nine gynogenic plantlets showing abnormal development were diploid. Therefore, formation of gynogenic plantlets with abnormal phenotypes was likely due to genetic factors. Results obtained from this study suggest use of DH technology in the production of homozygous A. tuncelianum inbreeds in variety improvement programs.

scholarly journals Improved in vitro rooting and acclimatization of “Violetta” Artichoke and “Green Globe” Artichoke

2021 ◽  
Vol 19 (1) ◽  
pp. 129-145
Author(s):  
Hoang Dac Khai ◽  
Nguyen Thi Nhu Mai ◽  
Hoang Le Lan Anh ◽  
Nguyen Nhu Minh Nguyet ◽  
Ho Viet Long ◽  
...  
Keyword(s):  
Large Scale ◽  
Economic Value ◽  
Shoot Multiplication ◽  
In Vitro Rooting ◽  
Globe Artichoke ◽  
Plantlet Production ◽  
Plantlet Quality ◽  
Number Of Shoots

Artichoke (Cynara scolymus L.), a medicinal plant with high economic value, contains high levels of phenolic compounds; especially cynarine, which plays an important role in preventing cancer, cardiovascular disease, osteoporosis, diabetes and neurodegeneration, etc. Currently, Artichoke micropropagation has achieved some success; however, the rooting efficiency and plantlet quality are still limited. In this study, improving the quality of Artichoke plantlet related to the shoot quality and suitable substrates in in vitro rooting stage was studied on “Violetta” Artichoke (VA) and “Green Globe” Artichoke (GA). The results showed that shoots (1.5 cm) cultured on MS medium supplemented 0.5 mg/L KIN were most suitable to shoot multiplication of VA with the number of shoots/explant (3.67 shoots), number of shoots ≥ 2 cm (3 shoots); while, 1.0 mg/L BA was suitable to shoot multiplication of GA (5.33 shoots; 5.00 shoots; respectively) after 4 weeks of culture. Besides, the in vitro rooting was improved using 8 g/L commercial agar for VA; meanwwhile, 3 g/L gelrite for GA. In addition, the nylon bag culture system (120 mm × 250 mm) has potential in plantlet production (15 plants/bag) and can be applied for large scale micropropagation. In addition, VA and GA plantlets derived from in vitro culture gave the good acclimatization, growth and development after 8, 12 and 20 weeks cultivating at the green house conditions.

Influence of explant slicing and cytokinin concentration on in vitro plantlet production ofMusasp.AAB‘Agbagba’

2018 ◽  
pp. 427-432
Author(s):  
S.O.S. Akinyemi ◽  
C.O. Esuola ◽  
O.B. Fajinmi ◽  
P.E. Akin-Idowu
Keyword(s):  
Plantlet Production

Effets de rayons γ 60Co sur des vitroplants haploïdes et diploïdes de Gerbera jamesonii

1987 ◽  
Vol 65 (10) ◽  
pp. 2074-2083 ◽  
Author(s):  
M.-A. Dubuc-Lebreux ◽  
J. Vieth
Keyword(s):  
Optimal Dosage ◽  
Anthocyanin Content ◽  
Low Doses ◽  
Gerbera Jamesonii ◽  
Plantlet Production ◽  
Overall Evaluation ◽  
Total Growth ◽  
Γ Rays ◽  
High Doses

Gerbera jamesonii ‘Super Gerbera’ explants were irradiated in vitro with 60Co γ-rays (8–100 Gy). Some received a single dose, while others received two or three at successive multiplication cycles. Several parameters were measured after 6 weeks of in vitro culture, to compare the effects of irradiation on haploid and diploid clones. Productivity is linked to the ploidy level, whereas total growth is not. Total growth inhibition is proportionate to the given dose. Callus and plantlet growth are either inhibited or stimulated, depending on the type of treatment. Callus growth is stimulated by low doses, which induce the transformation of apices into calli; treatment of successive cycles with low doses (20 and 30 Gy for diploids, 10 and 15 Gy for haploids) ensures better plantlet development. High doses inhibited growth of calli and plantlets up to the 50% level. Variations in morphology and in chlorophyll and anthocyanin content were observed among haploid and diploid irradiated explants. Plantlet production is a good criterion for an overall evaluation. Optimal dosage to ensure 50% growth and multiplication rates is estimated at 26 Gy for diploid clones and 16 Gy for haploid clones.

scholarly journals Cryopreservation of Arecanut (Areca catechu L.) Embryogenic Calli by V Cryo-plate Method

2021 ◽  
pp. 76-82
Author(s):  
Kilingar Subrahmanya Muralikrishna ◽  
Kalathil Kundanchery Sajini ◽  
Pulikuthi Kavya ◽  
Krishna Prakash ◽  
Abdulla Abdulla Sabana ◽  
...  
Keyword(s):  
Crop Improvement ◽  
Basal Medium ◽  
Start Codon ◽  
Embryogenic Calli ◽  
Immature Inflorescence ◽  
Plate Technique ◽  
Plantlet Production ◽  
Scot Marker ◽  
Palm Species

Aims: Arecanut, a perennial palm species of Arecaceae family, has huge commercial value, and is grown mainly for its masticatory nuts. The ever-increasing demand for uniform quality plantlets from growers necessitates putting in place In vitro mass multiplication and other crop improvement programmes. The present study was carried out to standardize the procedure for cryopreservation of embryogenic calli of arecanut, derived from immature inflorescence cultures, by vitrification based cryo-plate technique. Study Design: Completely randomized design (CRD) with three replications. Place and Duration of Study: ICAR-Central Plantation Crops Research Institute, Kerala, India during 2019. Methodology: The embryogenic calli were precultured in Eeuwen's Y3 basal medium supplemented with sucrose (0.2, 0.3 and 0.4 M) for three days. Explants were affixed on cryo-plates and later dehydrated using plant vitrification solution 3 (PVS3) for 30 min. Cryoplates were inserted in cryovials and cryopreserved. Explants with no cryostorage served as control. Explants were rewarmed quickly in a water bath (40ºC) for 2 min and treated with unloading solution and cultured on recovery medium. Results: The results showed 8-10 % recovery of embryogenic calli that resulted in normal plantlet production. The clonal fidelity studies, using Start Codon Targeted (SCoT) marker, showed no variation of cryopreserved calli in comparison to the original calli. Conclusion: This preliminary study demonstrated the successful use of vitrification (V) cryo-plate technique in cryopreservation of embryogenic calli of arecanut. With better recovery percentage, the optimal concentration of sucrose in the preculture medium was found to be 0.3 M. Desiccation in PVS3 solution for 30 min had no adverse effect.

scholarly journals Young capitulum as important explant in in vitro mass propagation of gerbera (Gerbera jamesonii)

2020 ◽  
Vol 12 (2) ◽  
pp. 264-276
Author(s):  
Budi WINARTO ◽  
Kurnia YUNIARTO ◽  
Rudy SOEHENDI
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
Leaf Length ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Plantlet Production ◽  
Half Strength ◽  
Flower Stage ◽  
The One

A new route of in vitro propagation of gerbera selected clones was successfully established using young capitula in tight buds and buds that were started to unfold stage as explant source. The one-fourth pieces of young capitula of tight flower stage and half-strength MS medium containing 0.25 mg/l BAP was the suitable for initiation and produced higher number of shoots per explant up to 3.8 shoots. The results were improved by culturing the one-fourth piece of 01.092 capitulums on MS medium fortified by 0.2 mg/l BAP and 0.02 mg/l NAA producing the highest shoot formation up to 8.5 shoots per explant with 28.7 leaves per explant and 2.1 cm leaf length. High multiple shoots were determined in third to fourth subculture periods and reduced thereafter with high multiplication rate noted on 01.092 clone. Shoots were easily rooted on half-strength MS medium supplemented with 2 g/l activated charcoal. Plantlets were transferred to ex vitro condition with 96.4% survivability of 03.045 clone using Cycas rumphii bulk and cocopeat (1:1, v/v) under spraying 1 g/l Growmore (32N:10P:10K) solution once week periodically. The route has high potential applied in qualified plantlet production for other Gerbera’s due to high shoots produced up to 35 shoots per whole young capitulum used. 

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