Colorimetric Determination Of Albumin In Micellar Extraction System Mо(VI) – Bromopyrogallol Red – Triton X-100

2015 ◽  
Vol 10 (2) ◽  
pp. 67-72
Author(s):  
M. Rohoza ◽  
◽  
S. Kulichenko ◽  

Author(s):  
Elmira I. Tsygulyova ◽  
◽  
Sergei Yu. Doronin ◽  

For the efficient preconcentration of azo compounds – products of the interaction of 4-nitrophenyldiazonium with thymol, a system 4-nitroaniline (4-NA) – NO2- – Triton X-100 – NaOH – ethanol has been proposed. The optimal conditions for the formation of micellarsaturated phases of the system under study have been established: 3.10-4 M 4-NA – 3.10-4 M NO2- – 5% Triton X-100 – 2.8 M NaOH – 10 vol. % С 2Н5ОН. A spectrophotometric study of the above system has been carried out. A linear dependence was built in the coordinates A (at λ max = 552 nm) vs с(thymol), which is described by an equation of the form A = f(c), A = 26291c + 0.02; R2 = 0.997. The range of the determined contents of thymol is (2·10-6 – 4·10-5) mol/l. A technique for the colorimetric determination of thymol in aqueous media (color channel G) has been developed. The intensity of the channel G chromaticity (IG) is linearly dependent on pc(thymol) in accordance with the equation IG = 54.2pc – 267, R 2 = 0; the lower limit of the determined contents of thymol is 1.10-6 mol/l, which is two times less than in the variant of its spectrophotometric determination. The profiles of petal diagrams in the color coordinates of the RGB CMYK model have been constructed; the dependences of their area (S) and perimeter (P) on the thymol concentration have been obtained (P: y = 278x – 10.13; R 2 = 0.97; S: y = 20182x – 87649, R 2 = 0.99).



2020 ◽  
Vol 75 (6) ◽  
pp. 726-732
Author(s):  
S. Yu. Doronin ◽  
E. S. Zhestovskaya ◽  
E. I. Tsyguleva


2016 ◽  
Vol 8 (39) ◽  
pp. 7141-7149 ◽  
Author(s):  
A. M. Hernández-Martínez ◽  
C. Padrón-Sanz ◽  
M. E. Torres-Padrón ◽  
Z. Sosa-Ferrera ◽  
J. J. Santana-Rodríguez

A new method has been developed for the extraction and determination of the heavy metals Ni, Cu, Cr, Pb, and Cd in mussels (Mytilus galloprovincialis) and polychaetes (Nereis diversicolor), using biodegradable mixed-micelles (SDS and Triton X-100) as the extractant, and GF-AAS analysis.





1996 ◽  
Vol 124 (1-2) ◽  
pp. 43-47 ◽  
Author(s):  
Li Jia ◽  
Guixi Zhao ◽  
Huaigong Wang
Keyword(s):  


2006 ◽  
Vol 61 (7) ◽  
pp. 638-640 ◽  
Author(s):  
D. G. Gambarov ◽  
F. G. Khalilova ◽  
Kh. D. Nagiev


1998 ◽  
Vol 20 (6) ◽  
pp. 199-203 ◽  
Author(s):  
K. A. Holm ◽  
D. M. Nielsen ◽  
J. Eriksen

An automated Cobas Fara method was developed determining the activity of recombinant M. thermophila laccase (rMtL). The chromogenic substrate used was syringaldazine. Under aerobic conditions, rMtL catalyses the oxidation of syringaldazine forming tetrametoxy-azo bis methylene quinone. The developed violet colour was measured kinetically at 530 nm as an expression of the enzyme activity, rMtL is a very sensitive oxidoreductase, therefore many factors had to be carefully controlled in order to get a robust analytical assay. In order to stabilize rMtL, PEG 6000 was added to the enzyme dilution medium. Furthermore, Triton X-I00 was included in the enzyme incubation solution.The analytical as well as technical conditions have been optimized, resulting in a method with good precision, sensitivity and speed of analysis. The Michaelis-Menten constant, Km, was determined to be 22μM syringaldazine. LOQ was determined to be 0.010 Uml-1, LOD to be 0.0002 Uml-1The analytical range of the enzyme dilution curve was from 0.01 to 0.044 Uml-1The repeatability was 1.9%, the reproducibility 3.1%. Testing the robustness of the method showed that the most sensible factors in the rMtL analysis in decreasing range were: incubation temperature, concentration of Triton X-I00, molarity and pH of the incubation buffer, and finally the concentration of syringaldazine.



1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg


Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
I Svinyarov ◽  
A Nedelcheva ◽  
MG Bogdanov


2013 ◽  
Vol 12 (7) ◽  
pp. 460-465
Author(s):  
Sameer Amereih ◽  
Zaher Barghouthi ◽  
Lamees Majjiad

A sensitive colorimetric determination of fluoride in drinking water has been developed using a polymeric zirconium complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline as fluoride reagents. The method allowed a reliable determination of fluoride in range of (0.0-1.5) mg L-1. The molar absorptivity of the complex formation is 7695 ± 27 L mol-1 cm-1 at 460 nm. The sensitivity, detection limit, quantitation limit, and percentage recovery for 1.0 mg L-1 fluoride for the proposed method were found to be 0.353 ± 0.013 μg mL-1, 0.1 mg L-1, 0.3 mg L-1, and 101.7 ± 4.1, respectively.



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