Effect of Ozone Pretreatment on the Microleakage of Pit and Fissure Sealants

Objective: This study investigated the effect of ozone pretreatment on the microleakage and marginal integrity of pit and fissure sealants placed with or without a self-etch 6th generation adhesive. Study Design: Freshly-extracted, human third molars were randomly assigned into two main groups (n=48):Group A: Fissures were pretreated with ozone; Group B: Fissures were left untreated. The teeth were further randomly divided into two subgroups (n=24/each) so that half of teeth were sealed with a conventional fissure sealant (Fissurit F, Voco, Germany), while the remaining half received the same sealant bonded with a self-etch adhesive (Clearfil Protect Bond, Kuraray, Japan). Following thermal cycling (1000X), the specimens were subjected to dye penetration within 0.5% basic fuchsin for 24h. The extent of dye penetration was measured by image analysis. Kruskal Wallis and Mann-Whitney U tests were used for statistical analysis of the data (p=0.05). Two randomly-selected sections from each group were observed under SEM.Results: In all groups, ozone pretreatment significantly reduced the extent of microleakage (p<0.001). SEM investigation demonstrated better adaptation of the sealants in ozone-pretreated groups. Clearfil Protect Bond did not improve the marginal seal of Fissurit F (p>0.05). Conclusion: Ozone pretreatment favorably affected the marginal sealing ability of the tested fissure sealants.

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Comparison of successful outcome of pit and fissure sealants bonded with etch-and-rinse adhesive and self-etch adhesive system

Technology and Health Care ◽

10.3233/thc-202679 ◽

2021 ◽

pp. 1-7

Author(s):

Jiand Malik Baloch ◽

Muhammad Adeel Ahmed ◽

Azeem Ul Yaqin Syed ◽

Muhammad Zohaib Younus ◽

Muhammad Owais Nasim ◽

...

Keyword(s):

Adhesive System ◽

Successful Outcome ◽

Fissure Sealants ◽

Pit And Fissure Sealants ◽

Etch And Rinse ◽

Molar Teeth ◽

Group A ◽

Group B ◽

Self Etch

BACKGROUND: Dental caries is one of the most common diseases around the world. The newly erupted permanent posterior teeth are at great risk due to the complex occlusal morphology of pits and fissures, infraocclusion and incomplete maturation of enamel. Pit and fissure sealants are the treatment of choice in such conditions. OBJECTIVES: To compare the successful outcome of pit and fissure sealants bonded with etch-and-rinse adhesive system and self-etch adhesive systems in newly erupted permanent first molars. METHODS: This randomized clinical trial was conducted at the Fatimah Jinnah Dental Hospital from June 2017 to May 2019. Children aged between 6 to 9 years who fulfilled the inclusion criteria were recruited for this study after parents’ consent. Patients were randomly divided into two groups by the lottery method. Thirty patients with 60 first molar teeth in Group A were treated with etch-and-rinse adhesive system, while the other thirty patients with 60 first molar teeth in Group B were treated with self-etch adhesive system. After adhesive placement, all children received the same fissure sealants in newly erupted maxillary and mandibular permanent first molar. Each group was clinically evaluated at 6 and 12 months follow-up visit. Modified United States Public Health Service (USPHS) criteria were used for direct clinical evaluation of successful outcomes such as sealant retention and marginal discoloration. RESULTS: Successful outcome was statistically significant in Group B compared to Group A (60% vs. 30% p= 0.020) at 12 months follow-up. Stratification analysis was performed with respect to age in which a successful outcome was not significant (p= 0.43) between groups for 6 to 7 years of age, while it was significant (p= 0.012) between groups in 8 to 9 years of age children. CONCLUSION: Self-etch adhesive system is better than etch-and-rinse adhesive system in terms of long-term sealant retention. In addition, the bonding procedure is simple with relatively short clinical application time therefore it would offer great benefit to clinicians in treating pediatric patients.

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An in vitro study of microleakage of pit and fissure sealants in the presence of occlusal forces

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.24.4.w725246855722u72 ◽

2000 ◽

Vol 24 (4) ◽

pp. 273-278 ◽

Cited By ~ 1

Author(s):

Christina Zervou ◽

Eileen Doherty ◽

Athanasios Zavras ◽

George White

Keyword(s):

Cross Section ◽

In Vitro Study ◽

Fissure Sealants ◽

Pit And Fissure Sealants ◽

Dye Penetration ◽

Wisdom Teeth ◽

Group A ◽

Group B ◽

Group D

The purpose of this study was to evaluate the effect of load on microleakage of pit and fissure sealants. In the first phase of the study load of maximal force (F = 880 N) on central and peripheral enamel was applied. Three groups of ten specimens were tested for microleakage. Group A no application of load, Group B application of load on the central pit of the tooth, and Group application of load on the peripheral enamel 2.5 mm away from the margin of the tooth, at the level of dentinoenamel junction. The results of that study showed significant increase of microleakage on the peripheral sealed enamel, as well as enamel microcracks. So, in the phase II sixty wisdom teeth were sealed with Ultra seal XT plus and load of different magnitude was applied on the peripheral enamel. The specimens were thermocycled for 500 cycles at 5°C and 55°C with a dwell of 30 seconds at each temperature prior to load application. Six groups of ten specimens were tested for microleakage under load. The force was applied at the peripheral enamel, 2.5 mm away from the margin of the tooth, at the level of dentin-enamel junction in cross-section view. Group A: F = 0 N; Group B: F = 300 N; Group C: F = 400 N; Group D: F = 500 N; Group E: F = 600 N; Group F: F = 700 N. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underlying fissure. A linear regression statistics was used with R-squared of 0.31. The groups with load applied (B, C, D, E, F) showed statistically significant results (p<0.05) compared with group A (F = 0 N). Both, the magnitude of the force and tooth morphology may influence the degree of microleakage observed after the placement of sealants.

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Microleakage of a Self-Adhesive Flowable Composite, a Self-Adhesive Fissure Sealant and a Conventional Fissure Sealant in Permanent Teeth with/without Saliva Contamination

Frontiers in Dentistry ◽

10.18502/fid.v14i4.2082 ◽

2019 ◽

Author(s):

Zohre Sadat Hosseinipour ◽

Alireza Heidari ◽

Mehdi Shahrabi ◽

Kiana Poorzandpoush

Keyword(s):

Practical Method ◽

Permanent Teeth ◽

Third Molars ◽

Fissure Sealants ◽

Fissure Sealant ◽

Dye Penetration ◽

Flowable Composite ◽

Saliva Contamination ◽

Vertise Flow

Objectives: Application of fissure sealants is a practical method for prevention of occlusal pit and fissure caries. Microleakage is an important factor affecting the success of fissure sealant treatment. This study aimed to assess the microleakage of a self-adhesive flowable composite, a self-adhesive fissure sealant and a conventional fissure sealant in permanent teeth with/without saliva contamination. Materials and Methods: This in vitro, experimental study evaluated 108 extracted human third molars, which were randomly divided into six groups (n=18) of control, Denu-Seal conventional fissure sealant applied on etched enamel with/without saliva contamination, Vertise Flow self-adhesive flowable composite applied on rinsed and dried enamel with/without saliva contamination and Prevent Seal self-adhesive fissure sealant applied on rinsed and dried enamel with/without saliva contamination. Microleakage was assessed using the dye penetration technique and subsequent observation of samples under a stereomicroscope. Data were analyzed using the Kruskal-Wallis and Tukey’s test. Results: Microleakage of the conventional fissure sealant with/without saliva contamination was significantly lower than that of other groups (P<0.05). Conclusion: Microleakage of the conventional fissure sealant is less than that of self-adhesive fissure sealant and self-adhesive composite, irrespective of saliva contamination.

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Laser versus LED polymerization: Comparison of in vitro preventive sealing procedures

Journal of Applied Biomaterials & Functional Materials ◽

10.1177/2280800018816013 ◽

2019 ◽

Vol 17 (1) ◽

pp. 228080001881601

Author(s):

Filiberto Mastrangelo ◽

Isabella Perraro ◽

Sabrina Mattia ◽

Giuseppe Troiano ◽

Khrystyna Zhurakivska ◽

...

Keyword(s):

Laser Diode ◽

Tooth Enamel ◽

In Vitro Study ◽

Third Molars ◽

Fissure Sealant ◽

Microscopic Evaluation ◽

Group A ◽

Group B ◽

Metallization Process

Introduction: The technique of sealing is a widely accepted procedure for prevention of caries. The aim of our in vitro study was to compare the effect of two different curing units (traditional LED source and innovative laser diode lamp) on the integrity of fissure sealant material and its interface with tooth enamel. Materials and methods: Sixty healthy third molars were randomly assigned to two groups. In group A were teeth intended for polymerization by LED B lamp, and group B comprised teeth to be polymerized by an innovative laser diode. Both groups were treated with the traditional sealing technique, subjected to a metallization process, and analyzed by scanning electron microscope. Results: Micro-gaps between the sealant and the enamel were found in specimens in both A (43%) and B (40%) groups ( p=0.793), and sealant shrinkage was seen. Significant differences between the groups emerged in the percentage of perimetric micro-erosion sites (80% vs. 100%, p=0.010) and the presence of holes and micro-bubbles on the sealant surface (21% vs. 63%, p=0.001). Conclusions: Although macroscopic clinical polymerization occurred with both instruments, the microscopic evaluation showed significant differences between the studied groups in terms of perimetric micro-erosion sites and micro-bubbles, which were higher in laser-cured samples.

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Microleakage of a Self-Adhesive Flowable Composite, a Self-Adhesive Fissure Sealant and a Conventional Fissure Sealant in Permanent Teeth with/without Saliva Contamination

Frontiers in Dentistry ◽

10.18502/fid.v16i4.2082 ◽

2019 ◽

Author(s):

Zohre Sadat Hosseinipour ◽

Alireza Heidari ◽

Mehdi Shahrabi ◽

Kiana Poorzandpoush

Keyword(s):

Practical Method ◽

Permanent Teeth ◽

Third Molars ◽

Fissure Sealants ◽

Fissure Sealant ◽

Dye Penetration ◽

Flowable Composite ◽

Saliva Contamination ◽

Vertise Flow

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A Comparative Evaluation of Microleakage in Class V Composite Restorations

International Journal of Dentistry ◽

10.1155/2014/685643 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Sujatha Gopal Sooraparaju ◽

Pavan Kumar Kanumuru ◽

Surya Kumari Nujella ◽

Karthik Roy Konda ◽

K. Bala Kasi Reddy ◽

...

Keyword(s):

Composite Resin ◽

Wilcoxon Test ◽

Basic Fuchsin ◽

Class V ◽

Bonding Agents ◽

Dye Penetration ◽

Flowable Composite ◽

Group A ◽

Nanohybrid Composite ◽

Group B

Aim. To compare and evaluate the microleakage in class V lesions restored with composite resin with and without liner and injectable nanohybrid composite resin.Materials and Methodology. 60 class V cavities were prepared in 30 freshly extracted teeth. After etching and application of bonding agents these cavities were divided into three groups: Group A (n=20)—restored with composite resin, Group B (n=20)—flowable composite resin liner + composite resin, and Group C (n=20)—restored with injectable composite resin. After curing all the specimens were subjected to thermocycling and cyclic loading. Specimens were stained with 0.5% basic fuchsin and evaluated for dye penetration.Results. Results are subjected to Kruskal Wallis and Wilcoxon test.Conclusion. Within the limitations of this study, none of the three materials were free from microleakage. All the three materials showed more microleakage at gingival margins compared to occlusal margins. Among all the groups G-ænial Flo showed the least microleakage at the gingival wall.

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Influence of irrigating agents on fiber postpush-out bond strength to radicular dentin sections with the different adhesive system

European Journal of Dentistry ◽

10.4103/ejd.ejd_280_16 ◽

2017 ◽

Vol 11 (03) ◽

pp. 380-384 ◽

Cited By ~ 1

Author(s):

Satheesh B. Haralur ◽

Asim Nasser A. Alasabi ◽

Sultan A. Abohathrah Al Qahtani ◽

Saeed Musleh S. Alqahtani

Keyword(s):

Bond Strength ◽

Ethylenediaminetetraacetic Acid ◽

Adhesive System ◽

Negative Influence ◽

Significant Difference ◽

Group A ◽

Coronal Section ◽

Group B ◽

Self Etch ◽

Push Out

ABSTRACT Objective: The objective of this study is to evaluate the effect of irrigating agents on push-out bond strength of resin postcemented with various adhesive systems at different radicular dentin sections. Materials and Methods:Sixty single-rooted premolar teeth were root canal treated, subsequently decorated at cementoenamel junction. The endodontic postspace was irrigated with 5.25% sodium hypochlorite (NaOCl) and 17% ethylenediaminetetraacetic acid (EDTA) for Group A (n = 30) and Group B (n = 30), respectively. The sample from each group was subdivided into three groups (10) according to luting protocol of etch-wash, self-etch, and self-adhesive. Individual teeth with cemented resin postsamples were sectioned into coronal, middle, and apical segments. Subsequently, subjected for pushout bond strength test by applying a load at 0.5 mm/min speed. The data were analyzed by three-way analysis of variance and Tukey comparison test at a significant difference level of 0.05.Results: The coronal section with 5.25% NaOCl irrigation and self-etch luting protocol provided the highest push out strength at 16.282 Mpa. The etch-wash luting protocol in both irrigations showed the lesser bond strength at 8.273 and 8.493 MPa, respectively, in coronal section.Conclusions: The self-etch adhesive system showed better push out bond strength and 17% EDTA had a negative influence on self-etch bond strength. The coronal sections had highest bond strength in comparison with apical radicular dentin sections.

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A comparison of microleakage in self-etch fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems

European Journal of Dentistry ◽

10.4103/ejd.ejd_63_18 ◽

2018 ◽

Vol 12 (02) ◽

pp. 242-246 ◽

Cited By ~ 1

Author(s):

Azam Nahvi ◽

Alireza Razavian ◽

Hoorieh Abedi ◽

Jamshid Yazdani Charati

Keyword(s):

In Vitro Study ◽

Conventional Technique ◽

Single Bond ◽

Adhesive Systems ◽

Fissure Sealants ◽

Fissure Sealant ◽

Acid Etch ◽

Self Etch ◽

Group 1

ABSTRACT Objective: The present study was conducted to compare microleakage in self-etching fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems. Settings and Design: This experimental in vitro study was conducted on 60 healthy third molars extracted from humans. The first group received Acid etch + Clinpro sealant, the second group received Acid etch + Single bond 2 + Clinpro sealant, the third group received Single bond universal (self-etching bonding) + Clinpro sealant, and the fourth group received prevent seal self-etching sealant. Materials and Methods: An incision was made on the teeth after they were immersed in methylene blue 5%. The samples were then examined under a stereomicroscope and the dye penetration rate was measured based on the Williams and Winter criteria. Statistical Analysis Used: The Kruskal–Wallis and Mann–Whitney tests were used for data analysis in SPSS-18 (P < 0.05). Results: Group 1 which was treated with the conventional technique (acid + fissure sealant) had the highest rate of microleakage compared to Groups 2 and 3 (P < 0.001). Conclusion: The results showed that the use of bonding results in a significant reduction in the microleakage of fissure sealants. The microleakage caused when using self-etch fissure sealant was not different from that caused by the use of the conventional method.

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Minimal Invasive Treatment for Defective Restorations: Five-Year Results Using Sealants

Operative Dentistry ◽

10.2341/12-062c ◽

2013 ◽

Vol 38 (2) ◽

pp. 125-133 ◽

Cited By ~ 26

Author(s):

J Martin ◽

E Fernandez ◽

J Estay ◽

VV Gordan ◽

IA Mjor ◽

...

Keyword(s):

Low Frequency ◽

Treated Group ◽

Recall Rate ◽

Marginal Adaptation ◽

Invasive Treatment ◽

Secondary Caries ◽

Fissure Sealant ◽

Significant Difference ◽

Group A ◽

Group B

SUMMARY Replacement of dental restorations has been the traditional treatment for restorations that are defective. In this five-year randomized clinical trial, restorations with localized marginal defects were treated with sealants. Thirty-two patients (mean age, 26.8 years) with 126 Class I and Class II restorations with defective margins (amalgam n=69 and resin-based composite n=57) were recruited. Treatment was seal with pit and fissure sealant on localized marginal defects (group A: n=43) and was compared with total restoration replacement (group B: n=40) and untreated restorations (group C: n=43) as negative and positive controls. Restorations were assessed by two examiners using the modified US Public Health Service criteria, observing five clinical parameters: marginal adaptation, roughness, marginal stain, teeth sensitivity, and secondary caries at baseline and at five years after treatment. At the five-year recall examination, 23 patients with 90 restorations (71.4% recall rate) were examined. A significant improvement was observed in the marginal adaptation of the restorations in group A compared with group B. None of the treated group showed trends to downgrade in any parameter. Tooth sensitivity and secondary caries showed a low frequency in all groups. No significant difference in marginal adaptation of the restorations was found between amalgam and resin-based composite restorations (p=0.191). This study demonstrated that marginal sealing of restorations is a minimally invasive treatment that may be used instead of the replacement of restorations with localized marginal defects.

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SEALING ABILITY OF MINERAL TRIOXIDE AGGREGATE, CALCIUM PHOSPHATE CEMENT, AND GLASS IONOMER CEMENT IN THE REPAIR OF FURCATION PERFORATIONS

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2014.16 ◽

2013 ◽

Vol 56 (3) ◽

pp. 97-103 ◽

Cited By ~ 3

Author(s):

Prabath Singh ◽

Joseph Paul ◽

Abdul Aziz Al-Khuraif ◽

Sajith Vellappally ◽

Hassan Suliman Halawany ◽

...

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Glass Ionomer Cement ◽

Mineral Trioxide Aggregate ◽

Glass Ionomer ◽

Dye Penetration ◽

Sealing Ability ◽

Group A ◽

Group B ◽

Ionomer Cement

Objectives: The purpose of this study was to evaluate the in vitro sealing ability of three repair materials. Mineral trioxide aggregate (MTA; Group A), calcium phosphate cement (CPC; Group B), and light cured glass ionomer cement (GIC; Group C) when used to repair the perforation created in the pulpal floor of fifty extracted human permanent molars. Materials and methods: Preparation of access openings and furcation perforations were done, and the teeth divided into five experimental groups (A, B, C) including two controls (D, E) with ten samples in each group randomly. Following the repair procedure, the pulp chambers and access openings were filled with composite resin and immersed in 2% methylene blue solution for 48 hours. The teeth were sectioned longitudinally and the linear dye penetration measured under a stereomicroscope. Results: The comparison of the linear length of micro-leakage (mm) among the experimental groups revealed no significant difference (p = 0.332). On calculating the percentage of depth of leakage to the total length of the perforation, it was observed that the mean leakage was 35.5% in Group A, 53.6% in Group B and the highest, 87.5% in Group C. The mean of leakage percentage was statistically significant by Kruskal-Wallis test (p = 0.003). The results indicated that the dye penetration used as furcation perforation repair material was least with mineral trioxide aggregate. Comparing the depth of penetration of dye, 50% of the Group A samples showed less than 25% of depth penetration. While 40% of Group B cases had more than 50% dye penetration. In our study, all Group C teeth had ≥ 50% dye penetration. Conclusions: The present study indicated that GIC had the greatest dye penetration followed by CPC and MTA. Mineral trioxide aggregate and calcium phosphate cement had comparatively better sealing ability than glass ionomer cement.

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