Concentration Polarization Quantification and Minimization in Cork Process Wastewater Ultrafiltration by an Ozone Pretreatment

Concentration polarization and membrane fouling have been identified as the main problems during the ultrafiltration treatment of cork processing wastewaters. These problems drastically reduce the permeate fluxes and, therefore, their potential applications. In this work, a soft ozonation pretreatment was applied to minimize these undesirable effects. A new systematic study was carried out for membranes with different molecular weight cut-offs and at different operating conditions to monitor and quantify the concentration polarization caused by the wastewater’s remaining ozonated compounds. Film theory was used to correlate the mass transfer coefficient, k, and the intrinsic rejection coefficient, f′, with the resistance introduced by concentration polarization. The ultrafiltration treatment was carried out under varying hydrodynamic operating conditions (circulating flow rates of 100–200 L/h) and transmembrane pressures (1–3 bar) for a set of four cellulose acetate membranes covering a wide range of molecular weight cut-offs (5000–100,000 Da) and hydraulic permeabilities (25–110 kg/h/m2/bar). The ozone pretreatment (at wastewater pH) reduced the phenolic content selectively (direct oxidation) by more than 50%, reducing membrane fouling and concentration polarization and increasing permeate fluxes (by 22–45%) and mass transfer coefficients (up to six times).

Chemical and Structural Changes of Ozonated Empty Fruit Bunch (EFB) in a Ribbon-Mixer Reactor

Agricultural wastes especially empty fruit bunch (EFB) are abundantly available to be utilized as a feedstock for biochemical synthesis or biofuel production. The components of the waste include lignin, hemicellulose and cellulose. Cellulose, the polymer of glucose, is the active component for producing bio-based chemicals. However, it is difficult to isolate cellulose since lignin, the most outer layer in the waste is recalcitrant. Therefore, the agricultural wastes need to be pre-treated prior to downstream processing. The aim of this study was to investigate the effect of ozone pretreatment on lignin degradation and total reducing sugar (TRS) yield. EFB was pre-treated using ozone gas in a ribbon-mixer reactor. The chemical and structural changes of ozonated EFB were analysed. The highest delignification obtained were 95.7 wt.% and TRS yield was enhanced to 84.9% at a moisture content of 40 wt.% with 60 g/m3 ozone concentration within one hour of reaction time. Both NMR and FTIR spectra conferred major peaks inferring higher lignin degradation could be achieved using ozonolysis. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

NH3-N and COD Reduction in Endek (Balinese Textile) Wastewater by Activated Sludge under Different DO Condition with Ozone Pretreatment

Nitrogen and organic matter are part of the pollutant causing eutrophication in freshwater. Textiles industry like Endek is the main source of Nitrogen and organic matter. Tis paper aims to know the degradation process of Ammonia-Nitrogen (NH3-N) and Chemical Oxygen Demand (COD) with ozone pretreatment in operated by different DO level activated sludge. There are 2 scenarios of treatments in this study: with ozone pretreatment (R1) and without ozone pretreatment (R2). Wastewater treatment began with the seeding and acclimatization process. This acclimatization study showed the efficiency degradation of NH3-N and COD by 17.7 and 27.5 %. Biological Oxygen Demand (BOD)/COD level increased with ozone pretreatment, from 0.25 to 0.38, COD/TKN level stated at 3.26. Ozone pretreatment reduced NH3-N and COD by 23.8 and 34.1 %. Wastewater treatment with activated sludge operated by different DO levels showed efficiency of 44.2 % (R1) and 68.2 % (R2). This ammonia elimination was capable of preventing eutrophication in the waterbody. The efficiency of NH3-N and Nitrogen organic degradation was indicated by TKN (Total Kjeldahl Nitrogen) levels: 87 % (R1) and 79 % (R2). The concentration of Nitrate (NO3-N) increased from 2.9 to 5.5 mg/L when DO reached 1 - 3 mg/L. COD reduction levels in this study were 17 % (R1) and 42.5 % (R2). Ozone pretreatment could make the efficiency of wastewater treatment qualified into standard quality.

Effect of ozonation on resistance of ovine and human erythrocytes to hypothermic storage

Long-term hypothermic storage of animal blood can lead to the loss of its quality and can cause complications in recipient animals after transfusion, so the search for new methods of increasing the preservation of erythrocytes after hypothermic storage continues. The article presents the data of the ozonation effect on the preservation rate of ovine and human erythrocytes during hypothermic storage with Alsever’s solution and mannitol medium. Hemolysis, osmotic fragility and distribution density of ovine and human erythrocytes by the sphericity index were determined at different stages of hypothermic storage. The ovine erythrocytes in the control had a lower osmotic resistance compared to human erythrocytes; however, their osmotic fragility did not change significantly after hypothermic storage for 8 weeks, and for human erythrocytes, it significantly increased. Storage of ovine and human erythrocytes longer than 8 weeks led to a sharp hemolysis, while the level of hemolysis of ovine erythrocytes was lower than that of human erythrocytes. Preservation of ozonated erythrocytes is higher than non-ozonated ones during prolonged hypothermic storage. The effect of ozonation on the preservation rate of red blood cells depended on the composition of the preservation media. Hypothermal storage of human erythrocytes in Alsever’s solution for up to 8 weeks led to a shift in the density of distribution according to the sphericity index towards spheroidization of cells; in a medium with mannitol, the number of flattened cell forms increased. After 8 weeks of hypothermic storage of ovine erythrocytes, most of the cells had high sphericity indices. Pretreatment of human and sheep erythrocytes with ozone allows the distribution of cells to be kept closer to the control during long-term storage, which is especially pronounced in mannitol medium. Ovine erythrocytes retained the ability to form rosettes with human T-lymphocytes after hypothermic storage for up to 12 weeks. Thus, ozone pretreatment and the use of mannitol as part of hypothermic storage medium could be an approach to improve the quality of preserved ovine erythrocytes.