Russian strains of group B streptococci are different in the content and organization of the PAI-A and PAI-A1 pathogenicity islands

2021 ◽  
Vol 70 (4) ◽  
pp. 65-72
Author(s):  
Evgenia V. Kuleshevich ◽  
Yury Y. Ilyasov ◽  
Dmitry S. Linnik ◽  
Anastasia A. Malchenkova ◽  
Olga N. Arzhanova ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Pathogenicity Island ◽  
Mobile Genetic Elements ◽  
Urogenital Tract ◽  
Pathogenicity Islands ◽  
Group B Streptococci ◽  
Clinical Strains ◽  
Group B ◽  
Tract Infections

Group B streptococci, or Streptococcus agalactiae, are the major cause of severe diseases in newborns and adults. The PAI-A and PAI-A1 pathogenicity islands containing the sspB1 and sspB1a genes, respectively, were found among group B streptococci mobile genetic elements. The presence of sspB genes correlates with urogenital tract infections. The aim of this study was to determine the frequency of group B streptococci strains with the PAI-A and PAI-A1 pathogenicity islands, circulating in Moscow, in comparison with strains from St. Petersburg. The sspB1 gene, and hence the PAI-A pathogenicity island, was not found in the genomes of strains from Moscow. The frequency of the sspB1a gene and the PAI-A1 pathogenicity island in the genomes of clinical strains was three times higher than in the genomes of colonizing strains. Thus, it can be assumed that the genes of the sspB family are more specific of group B streptococci colonizing pregnant women and newborns.

Comparison of culture with two different qPCR assays for detection of rectovaginal carriage of Streptococcus agalactiae (group B streptococci) in pregnant women

2011 ◽  
Vol 162 (5) ◽  
pp. 499-505 ◽  
Author(s):  
Nabil Abdullah El Aila ◽  
Inge Tency ◽  
Geert Claeys ◽  
Hans Verstraelen ◽  
Pieter Deschaght ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococci ◽  
Group B

scholarly journals Distribution of Pilus island and antibiotic resistance genes in Streptococcus agalactiae obtained from vagina of pregnant women in Yazd, Iran

2020 ◽  
Author(s):  
Mahdieh Nabavinia ◽  
Mohammad Bagher Khalili ◽  
Maryam Sadeh ◽  
Gilda Eslami ◽  
Mahmood Vakili ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Streptococcus Agalactiae ◽  
Antibiotic Resistance Genes ◽  
Invasive Disease ◽  
Group B Streptococci ◽  
Resistance Factors ◽  
Group B

Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B streptococci (GBS), in production of invasive disease in neonates, investigation regarding the pathogenicity and antibiotic resistance factors is necessary in selecting the appropriate therapeutic agents. Beside capsule, the pilus has been currently recognized as an important factor in enhancing the pathogenicity of GBS. Resistance of GBS to selected antibiotics is noticeably increasing which is mainly due to the anomalous use of these drugs for treatment. The aim of this study was to determine the prevalence of pili genes followed by antibiotic susceptibility of GBS, previously serotyped, isolated from pregnant women in the city of Yazd, Iran. Materials and Methods: Fifty seven GBS from pregnant women were subjected to multiplex PCR for determination of PI-1, PI-2a and PI-2b pilus-islands and simultaneously, the phenotype of antibiotic resistance to penicillin, tetracycline, erythromycin, clindamycin, gentamycin and levofloxacin was determined. Antibiotic resistance genes (ermA, ermB, mefA, tetM, int-Tn) were further diagnosed using PCR and multiplex PCR. Results: PI-1+PI-2a with 71.9%; followed by PI-2a (21.1%) and PI-2b (7%) were observed. PI-1+PI-2a in serotype III was (73.2%), serotype II, Ia, Ib and V were 12.2%, 9.8%, 2.4% and 2.4% respectively. GBS penicillin sensitive was 89.5% and 96.5% resistance to tetracycline. The frequency of resistance genes were as follows: tetM (93%), ermA (33.3%), ermB (8.8%), int-Tn (80.7%) and mefA (0). Conclusion: Majority of GBS contained PI-1+PI-2a. Hence presence of this pilus stabilizes the colonization, therefore designing a program for diagnosing and treatment of infected pregnant women seems to be necessary.

scholarly journals Rectal and Vaginal Eradication of Streptococcus agalactiae (GBS) in Pregnant Women by Using Lactobacillus salivarius CECT 9145, A Target-specific Probiotic Strain

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 810 ◽  
Author(s):  
Virginia Martín ◽  
Nivia Cárdenas ◽  
Sara Ocaña ◽  
María Marín ◽  
Rebeca Arroyo ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Pilot Trial ◽  
Probiotic Strain ◽  
Group B Streptococci ◽  
Lactobacillus Salivarius ◽  
In Vitro Characterization ◽  
Intrapartum Antibiotic ◽  
Group B

Streptococcus agalactiae (Group B Streptococci, GBS) can cause severe neonatal sepsis. The recto-vaginal GBS screening of pregnant women and intrapartum antibiotic prophylaxis (IAP) to positive ones is one of the main preventive options. However, such a strategy has some limitations and there is a need for alternative approaches. Initially, the vaginal microbiota of 30 non-pregnant and 24 pregnant women, including the assessment of GBS colonization, was studied. Among the Lactobacillus isolates, 10 Lactobacillus salivarius strains were selected for further characterization. In vitro characterization revealed that L. salivarius CECT 9145 was the best candidate for GBS eradication. Its efficacy to eradicate GBS from the intestinal and vaginal tracts of pregnant women was evaluated in a pilot trial involving 57 healthy pregnant women. All the volunteers in the probiotic group (n = 25) were GBS-positive and consumed ~9 log10 cfu of L. salivarius CECT 9145 daily from week 26 to week 38. At the end of the trial (week 38), 72% and 68% of the women in this group were GBS-negative in the rectal and vaginal samples, respectively. L. salivarius CECT 9145 seems to be an efficient method to reduce the number of GBS-positive women during pregnancy, decreasing the number of women receiving IAP during delivery.

scholarly journals Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran

2020 ◽  
Author(s):  
Leila Goudarzi ◽  
Mohammad Bagher Khalili ◽  
Mahmood Vakili ◽  
Maryam Sadeh
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Specific Pcr ◽  
Pcr Method ◽  
Group B ◽  
Tehran Area

Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.

scholarly journals Rapid identification of pregnant women heavily colonized with group B streptococci.

1983 ◽  
Vol 18 (3) ◽  
pp. 558-560 ◽  
Author(s):  
D E Jones ◽  
E M Friedl ◽  
K S Kanarek ◽  
J K Williams ◽  
D V Lim
Keyword(s):  
Pregnant Women ◽  
Rapid Identification ◽  
Group B Streptococci ◽  
Group B
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