scholarly journals Serological diagnosis of gonorrhea

2021 ◽  
Vol 25 (11) ◽  
pp. 1237-1238
Author(s):  
A. Dmitriev
Keyword(s):  
Clinical Research ◽  
Research Methods ◽  
Positive Reaction ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Diagnostic Methods ◽  
Serological Diagnosis ◽  
Fixation Reaction ◽  
Difficult Cases

Alf. Cohn (Derm. Ztschr. Bd. 55, H. 2) gives detailed instructions for the preparation of a specific antigen, patient serum, and also describes in detail the technique of the Bordet-Gengou reaction itself. The author received as a result of his observations with acute gonorrhea in men 40% pos. responses, for chronic 60% and for complicated 80-100%. The results are especially demonstrative in gonorrhoid arthritis (100%). It is important to use the reaction in doubtful cases, when microscopic and bacteriological examinations do not give the necessary answer for making a diagnosis. If people who do not have gonorrhea are vaccinated, the reaction may be positive. If a positive reaction persists for months or years, you need to look for a focus with gonococci. According to the author, the complement fixation reaction cannot replace the previously tested diagnostic methods, but it complements them. Together with bacteriological and clinical research methods, the reaction sometimes helps to understand the diagnosis of difficult cases.

A NON-SPECIFIC COMPLEMENT FIXATION REACTION DUE TO ION ANTAGONISM

1932 ◽  
Vol 7 (6) ◽  
pp. 585-595
Author(s):  
A. Hambleton
Keyword(s):  
Positive Reaction ◽  
Calcium Ions ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Physiological Saline ◽  
Tubercle Bacillus ◽  
Antagonistic Effects ◽  
Ion Antagonism ◽  
Calcium And Magnesium ◽  
Fixation Reaction

The complement fixation test for tuberculosis gives a non-specific or falsely positive reaction if a balanced physiological saline, akin to Tyrode's solution, replaces the plain saline used in the test. The balanced saline must contain both calcium and magnesium in appropriate concentration. The non-specific reaction is given most strongly by Petroff's whole bacillus antigen, and not at all by "fat-free" tubercle bacillus antigens. The main features of the reaction, and its relation to antagonistic effects of sodium and calcium ions, are indicated.

The Complement Fixation Reaction in Schistosoma japonicum with Cercarial Antigen prepared from Oncomelania hupensis

1935 ◽  
Vol 13 (1) ◽  
pp. 25-40
Author(s):  
Mary N. Andrews
Keyword(s):  
Schistosoma Japonicum ◽  
Positive Reaction ◽  
Complement Fixation ◽  
Clonorchis Sinensis ◽  
Alcoholic Extract ◽  
Human Beings ◽  
Oncomelania Hupensis ◽  
Weak Reaction ◽  
Negative Results ◽  
Fixation Reaction

1. The preparation of a cercarial antigen from infected livers of Oncomelania hupensis the intermediate host of Schistosoma japonicum in China is described.2. The sera of 49 human beings and five dogs have been investigated for the Schistosome complement fixation reaction, using as antigens Bilharzial cercarial antigen, and an alcoholic extract of the livers of Oncomelania hupensis infected with cercariae of S. japonicum.3. Thirty-seven of the 49 sera examined were from cases of Schistosomiasis, and of these 24 gave a positive complement fixation reaction, and 2 others a weak reaction.4. Of the five dogs examined three were infected with Schistosoma japonicum. These gave positive reactions.5. In 24 cases both antigens were used, and the results obtained were almost identical.6. The Bilharzial antigen was used in 33 cases including 25 cases of Schistosomiasis, of which 19 gave a positive reaction.7. In 40 cases the Oncomelania antigen was used. This series included 32 cases of Schistosomiasis of which 23 gave a positive reaction and 3 a weak positive reaction.8. Negative results were obtained with 50 strongly positive Wasserman sera, and with 2 cases of infection with Fasciolopsis buski, and two cases of Clonorchis sinensis. Ascaris and hookworm infestations also gave negative results.

Complement fixation reaction of Rift Valley fever virus

1950 ◽  
Vol 5 (5) ◽  
pp. 243-247
Author(s):  
Minoru MATSUMOTO ◽  
Saburo IWASA ◽  
Motosige ENDO
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Complement Fixation ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Fixation Reaction

scholarly journals Serological tests for the detection of antibodies to Mycoplasma gallisepticum in chickens and turkeys

1968 ◽  
Vol 66 (2) ◽  
pp. 249-267 ◽  
Author(s):  
F. T. W. Jordan ◽  
P. Kulasegaram
Keyword(s):  
Positive Reaction ◽  
Complement Fixation ◽  
Haemagglutination Inhibition ◽  
Mycoplasma Gallisepticum ◽  
Metabolic Inhibition ◽  
Agar Gel ◽  
Serological Tests ◽  
Anamnestic Response ◽  
Initial Infection ◽  
Gel Method

SUMMARYA comparison was undertaken of several serological tests in determining the response of chickens and turkeys experimentally infected with the A 514 strain of Mycoplasma gallisepticum.After a single intratracheal inoculation of chickens with a culture of the organism, the highest titres were obtained by the indirect complement fixation (ICF) test, followed by the tube agglutination (TA), haemagglutination inhibition (HI), slide agglutination (SA) and metabolic inhibition (MI) tests. By all these tests positive titres were observed within the first week and peak titres between the first and second weeks. At 5 months there was no positive reaction by the ICF test but most chickens gave positive readings by the TA, HI and SA tests for at least 14 months after infection, but turkey sera became negative by all tests after 3 months.A disadvantage of the ICF test was that sera up to a dilution of 1/8 and 1/16 for chicken and turkey respectively were anticomplementary, and in turkeys this masked the ICF titre, which presumably was low following one intratracheal inoculation. Titres in turkeys with the TA, HI and SA tests followed the pattern seen with chickens and were generally lower than those found by other workers probably because of the avirulent nature of the inoculum used.The WB test was the least sensitive of the agglutination tests but is useful as a flock test which can be undertaken on the farm.The MI test gave the lowest titres of all and antibodies could be detected for only 4 months following one intratracheal inoculation. Even with serum prepared by multiple inoculations in chickens the titre was never higher than 1/32 compared with 1/1024 for serum similarly prepared in rabbits.Precipitins were detected by the agar gel method in the sera of chickens and turkeys after two intratracheal inoculations but in only some of the chickens and none of the turkeys after one inoculation.By all tests higher titres were observed with chicken than turkey sera and antibodies persisted for a longer time.Re-infection of chickens when antibodies to the initial infection had become low, and of turkeys when antibodies were no longer detectable, gave rise to an anamnestic response with titres which were higher than before.Antiserum to M. gallisepticum prepared in chickens is comparable with that prepared in rabbits except for low titres by the MI test.

scholarly journals Texture analysis in perfusion images of prostate cancer—A case study

2010 ◽  
Vol 20 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Jacek Śmietański ◽  
Ryszard Tadeusiewicz ◽  
Elżbieta Łuczyńska
Keyword(s):  
Prostate Cancer ◽  
Texture Analysis ◽  
Medical Diagnosis ◽  
Diagnostic Methods ◽  
Tomographic Images ◽  
Perfusion Computed Tomography ◽  
Independent Verification ◽  
Cancerous Lesion ◽  
Difficult Cases

Texture analysis in perfusion images of prostate cancer—A case studyThe analysis of prostate images is one of the most complex tasks in medical images interpretation. It is sometimes very difficult to detect early prostate cancer using currently available diagnostic methods. But the examination based on perfusion computed tomography (p-CT) may avoid such problems even in particularly difficult cases. However, the lack of computational methods useful in the interpretation of perfusion prostate images makes it unreliable because the diagnosis depends mainly on the doctor's individual opinion and experience. In this paper some methods of automatic analysis of prostate perfusion tomographic images are presented and discussed. Some of the presented methods are adopted from papers of other researchers, and some are elaborated by the authors. This presentation of the method and algorithms is important, but it is not the master scope of the paper. The main purpose of this study is computational (deterministic and independent) verification of the usefulness of the p-CT technique in a specific case. It shows that it is possible to find computationally attainable properties of p-CT images which allow pointing out the cancerous lesion and can be used in computer aided medical diagnosis.

Succinate dehydrogenase, phosphatase * and leukocyte glycogen in the dynamics of adenovirus infection in newborns

1982 ◽  
Vol 63 (2) ◽  
pp. 52-53
Author(s):  
O. I. Pikuza ◽  
E. P. Gurevich
Keyword(s):  
Succinate Dehydrogenase ◽  
Complement Fixation ◽  
Hydrolytic Enzymes ◽  
The Body ◽  
Direct Immunofluorescence ◽  
Adenovirus Infection ◽  
Serological Study ◽  
Blood Leukocytes ◽  
Acid And Alkaline Phosphatase ◽  
Fixation Reaction

The objective of our study was to study the indicators of oxidative and hydrolytic enzymes of blood leukocytes in the dynamics of adenovirus infection in comparison with the duration of circulation of adenoviruses in the body. For this purpose, the activity of acid and alkaline phosphatase (AP and ALP), succinate dehydrogenase (SDH), the content of glycogen (G) in leukocytes, as well as the duration of the luminescence of adenoviral antigen in epithelial cells of the nose were determined by direct immunofluorescence. In addition, a serological study of paired blood sera from the same patients was carried out by staging a complement fixation reaction (CSC).

Assessment of Change after Long-Term Psychoanalytic Group Treatment: Presentation of a Field Study of Outpatients from Private Psychiatric Practice

2000 ◽  
Vol 33 (3) ◽  
pp. 373-396 ◽  
Author(s):  
Steinar Lorentzen
Keyword(s):  
Clinical Practice ◽  
Clinical Research ◽  
Field Study ◽  
Research Methods ◽  
Group Treatment ◽  
Private Practice ◽  
Experimental Studies ◽  
Effectiveness Study ◽  
Efficacy Studies

The main purpose of this article is to underline the importance of doing clinical research on long-term, dynamic group psychotherapy as it is carried out in practice (effectiveness study). After a review of the outcome literature, which mainly consists of experimental studies (efficacy studies), an effectiveness study from a private practice will be described with some preliminary results. Experiences from implementation of a research project in clinical practice are presented and the strengths and limitations of the two research methods are discussed.

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