Destructive effect of RNаse a on the SARS-CoV-2 virus (in vitro research)

2021 ◽  
Vol 21 (3) ◽  
pp. 131-134
Author(s):  
Ilya A. Morozov ◽  
Anatoly P. Godovalov ◽  
Denis A. Oborin
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Primary Diagnosis ◽  
Ribonuclease A ◽  
Medical Community ◽  
Destructive Effect ◽  
Environmental Objects ◽  
Tick Borne Encephalitis ◽  
Prevention And Treatment

BACKGROUND: For today, the most important and discussed issue in the professional medical community is the problem of prevention and treatment of a new coronaviral infection (COVID-19). The main reason for the non-decreasing increase in morbidity and mortality is the absence of an etiotropic drug. In our study, it is proposed to use a previously available drug for the treatment of tick-borne encephalitis, ribonuclease A, obtained from the pancreas of cattle. AIM: The aim of investigation was to study the antiviral activity of RNаse A against SARS-CoV-2 in in vitro experiments. MATERIALS AND METHODS: The experiment used samples of 50 patients with a confirmed (by PCR) primary diagnosis of a new coronoviral infection COVID-19. The preparation for the study was served by ribonuclease A (neoFroxx GmbH, Germany) at a concentration of 0.5; 1; 5 and 10 mg/ml, incubated at 4 and 37C, the exposure was 20 minutes, 20 hours. A set of reagents OTT-PCR-RV-SARS-CoV-2 (Syntol, Russia) was used as test systems. RESULTS: of the current study is the revealed antiviral activity of ribonuclease A at a minmal concentration of 0.5 mg/ml during incubation from 20 minutes to 20 hours, in the temperature range of 437C. CONCLUSIONS: The data obtained in the in vitro study confirmed the ability of ribonuclease A to destroy viral RNA, which suggests the possible use of the drug both for the treatment of patients and for the treatment of environmental objects.

scholarly journals In vitro activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus

2021 ◽  
Vol 66 (2) ◽  
pp. 123-128
Author(s):  
S. Ya. Loginova ◽  
V. N. Shсhukina ◽  
S. V. Savenko ◽  
S. V. Borisevich
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Viral Infections ◽  
Complete Suppression ◽  
Antiviral Efficacy ◽  
Prevention And Treatment ◽  
Proven Efficacy ◽  
High Antiviral Activity

Introduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency of finding drugs effective against this pathogen.The aim of this work was to evaluate the in vitro antiviral efficacy of human recombinant alpha-2b interferon (IFN-α2b) against SARS-CoV-2 virus.Material and methods. The experiments had been carried out on Vero Cl008, the continuous line of African green monkey (Chlorocebus sabaeus) kidney cells. The effectiveness of the drugs was assessed by the suppression of viral reproduction in vitro. The biological activity was determined using titration of a virus-containing suspension in a Vero Cl008 cell culture by the formation of negative colonies.Results. The antiviral efficacy of the IFN-α2b-based medications, which have a high safety profile and proven efficacy in the prevention and treatment of influenza and acute respiratory viral infections (ARVI), has been studied against the new pandemic SARS-CoV-2 virus in vitro experiments in Vero C1008 cell culture. IFN-α2b effectively inhibits the reproduction of the virus when applied both 24 hrs before and 2 hrs after infection. In the IFN-α2b concentration range 102–106 IU/ml a complete suppression of the reproduction of the SARS-CoV-2 virus had been demonstrated.Discussion. IFN-α2b demonstrated in vitro high antiviral activity against SARS-CoV-2. In addition, the substance has a high chemotherapeutic index (>1000).Conclusion. Medications for intranasal use based on IFN-α2b have high antiviral activity and are promising drugs for in vivo study in terms of prevention and treatment of COVID-19.

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

2018 ◽  
Vol 8 (2) ◽  
pp. 81-85 ◽  
Author(s):  
Abdi Hussein Hadun ◽  
James Mucunu Mbaria ◽  
Gabriel Oluga Aboge ◽  
Mitchel Otieno Okumu ◽  
Antony Letoyah Yiaile
Keyword(s):  
Herpes Simplex ◽  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Herpes Simplex Type

Cytotoxicity, Antibiotic Combination and Antiviral Activity of Papain Enzyme: In vitro study

2020 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Sara H. Mohamed ◽  
Walaa S. Mohamed ◽  
Mohamed N. F. Shaheen ◽  
Elmahdy M. Elmahdy ◽  
Mona I. Mabrouk
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibiotic Combination

Synthesis, Characterization and in Vitro Study of the Cytostatic and Antiviral Activity of New Polymeric Silver(I) Complexes with Ribbon Structures Derived from the Conjugated Heterocyclic Thioamide 2-Mercapto-3,4,5,6-tetra- hydropyrimidine

2004 ◽  
Vol 2004 (7) ◽  
pp. 1420-1426 ◽  
Author(s):  
Panagiotis C. Zachariadis ◽  
Sotiris K. Hadjikakou ◽  
Nick Hadjiliadis ◽  
Stavroula Skoulika ◽  
Adonis Michaelides ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ribbon Structures

scholarly journals Antiviral Properties of Water Extracts of Mycelium Inonotus rheades (Pers.) P. Karst. (1882) against the Virus of Tick-Borne Encephalitis Virus in vitro

2021 ◽  
Vol 6 (1) ◽  
pp. 55-59
Author(s):  
M. A. Khasnatinov ◽  
T. G. Gornostai ◽  
I. S. Solovarov ◽  
M. S. Polyakova ◽  
G. A. Danchinova ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Antiviral Agents ◽  
Encephalitis Virus ◽  
Water Soluble ◽  
Xylotrophic Basidiomycetes ◽  
Tick Borne Encephalitis ◽  
Wood Polysaccharides ◽  
Tick Borne Encephalitis Virus ◽  
The Relationship

Background. Tick-borne encephalitis virus is dangerous and widespread pathogen that is transmitted to humans through the bites of hard ticks. Wild fungi, such as xylotrophic basidiomycetes, are widely used in traditional medicine to treat the infectious diseases and are promising natural sources of new antiviral agents. It was previously shown that aqueous extracts from the mycelium of the Inonotus rheades (Pers.) P. Karst. (1882) fungus exhibit significant antiviral activity against tick-borne encephalitis virus, however, the mechanisms of this activity remain unclear.Aim. To analyze the relationship between the virucidal properties of I. rheades extract and the substrate on which the cultivation was carried out.Materials and methods. The mycelium was grown either in a standard liquid medium with wort or on wooden disks from birch. Extracts of water-soluble polysaccharides were prepared from both mycelium samples. The concentration of infectious tick-borne encephalitis virus was determined using the method of titration of plaque-forming components (PFU). Approximately 30 000 PFU of tick-borne encephalitis virus was mixed with an equal volume of corresponding I. rheades extract at concentration of 8 mg/mL and incubated for 30 min at 37 °C. Afterwards, the residual infectivity of tick-borne encephalitis virus was determined using the identical virus sample incubated with sterile water as a reference.Results. It was found that treatment of tick-borne encephalitis virus with extracts from I. rheades mycelium resulted in inhibition of the infectivity of the virus in the cell culture. However, the same strain of I. rheades, grown on medium with wort, did not exhibit antiviral properties.Conclusions. Virucidal substances are likely to be not the main metabolites of the mycelium of I. rheades, but are rather metabolized wood polysaccharides. Further research is needed to more accurately identify the active ingredients and assess their antiviral activity.

scholarly journals In Vitro Study of Picloxidine 0.05 % Antiviral Activity (on the Example of Adenovirus)

2020 ◽  
Vol 17 (3s) ◽  
pp. 634-639
Author(s):  
G. M. Chernakova ◽  
D. Yu. Maychuk ◽  
E. A. Klescheva ◽  
M. V. Mezentseva ◽  
L. I. Russu ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Evaluation of Antiviral Activities of Curcumin Derivatives against HSV-1 in Vero Cell Line

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Keivan Zandi ◽  
Elissa Ramedani ◽  
Khosro Mohammadi ◽  
Saeed Tajbakhsh ◽  
Iman Deilami ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
In Vitro Study ◽  
Vero Cell Line ◽  
Hsv 1

Antiviral drug resistance is one of the most common problems in medicine, and, therefore, finding new antiviral agents, especially from natural resources, seems to be necessary. This study was designed to assay the antiviral activity of curcumin and its new derivatives like gallium-curcumin and Cu-curcumin on replication of HSV-1 in cell culture. The research was performed as an in vitro study in which the antiviral activity of different concentrations of three substances including curcumin, Gallium-curcumin and Cu-curcumin were tested on HSV-1. The cytotoxicity of the tested compounds was also evaluated on the Vero cell line. The CC50 values for curcumin, gallium-curcumin and Cu-curcumin were 484.2 μg/mL, 255.8 μg/mL and 326.6 μg/mL, respectively, and the respective IC50 values 33.0 μg/mL, 13.9 μg/mL and 23.1 μg/mL. The calculated SI values were 14.6, 18.4 and 14.1, respectively. The results showed that curcumin and its new derivatives have remarkable antiviral effects on HSV-1 in cell culture.

scholarly journals Antiviral activity of Aloe vera against herpes simplex virus type 2: An in vitro study

2007 ◽  
Vol 6 (15) ◽  
pp. 1770-1773 ◽  
Author(s):  
Z Keivan ◽  
isup ◽  
Abbas Zadehsup Moloud ◽  
Sartavisup Kohzad ◽  
Rastiansup Zahra
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antiviral Activity ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Aloe Vera ◽  
In Vitro Study ◽  
Simplex Virus

In vitro Study of Antiviral Activity of Mycophenolic Acid on Brazilian Orthobunyaviruses

Intervirology ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 204-208 ◽  
Author(s):  
Márcia Cristina Livonesi ◽  
Ricardo Luiz Moro de Sousa ◽  
Luiz Tadeu Moraes Figueiredo
Keyword(s):  
Antiviral Activity ◽  
Mycophenolic Acid ◽  
In Vitro Study ◽  
Vitro Study
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