scholarly journals The influence of phacoemulsification on various corneal layers in patients with pseudoexfoliation syndrome assessed with confocal in vivo microscopy

2017 ◽  
Vol 10 (3) ◽  
pp. 29-34 ◽  
Author(s):  
Vitaly V Potemkin ◽  
Tatyana S Varganova ◽  
Irina V Terekhova ◽  
Elena V Ageeva
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Cataract Extraction ◽  
Pseudoexfoliation Syndrome ◽  
In Vivo Microscopy ◽  
Corneal Epithelial ◽  
Cellular Density ◽  
Epithelial Layers ◽  
The Impact

Phacoemulsification (PHACO) is the basic procedure of cataract extraction. Purpose. To assess the impact of PHACO on corneal epithelium layers in patients with pseudoexfoliation syndrome (PEX) by confocal in vivo microscopy. Methods. 24 patients with PEX syndrome and 18 patients without it were enrolled in the prospective study. In vivo confocal microscopy was performed with assessment of cellular density in corneal epithelial layers, degree of its desquamation, degree of Bowman membrane stiffening and dendritic cells density. Results. The epithelial cells density didn’t change significantly in groups. Confocal microscopy showed high density of dendritic cells and marked desquamation of the epithelium in patients with PEX (p < 0,05) after PHACO. (For citation: Potemkin VV, Varganova TS, Terekhova IV, Ageeva EV. The influence of phacoemulsification on various corneal layers in patients with pseudoexfoliation syndrome assessed with confocal in vivo microscopy. Ophthalmology Journal. 2017;10(3):29-34. doi: 10.17816/OV10329-34).

scholarly journals Assessment of Corneal subbasal nerve plexus using confocal in vivo microscopy in patients with pseudoexfoliation syndrome after phacoemulsification

2018 ◽  
Vol 11 (2) ◽  
pp. 13-18 ◽  
Author(s):  
Vitaly V. Potyomkin ◽  
Tatyana S. Varganova ◽  
Irina V. Terehova ◽  
Elena V. Ageeva
Keyword(s):  
Cataract Surgery ◽  
Main Group ◽  
Nerve Plexus ◽  
Pseudoexfoliation Syndrome ◽  
Control Group ◽  
In Vivo Microscopy ◽  
Before And After ◽  
The Impact ◽  
Subbasal Nerve Plexus

Phacoemulsification (PE) is the leading method of cataract surgery. Purpose. To assess the impact of PE on corneal subbasal nerve plexus in patients with pseudoexfoliation syndrome (PEX) using confocal in vivo microscopy. Methods. 42 patients (42 eyes) were enrolled in the study. The main group consisted of 24 patients (24 eyes) with PEX syndrome, and 18 patients (18 eyes) without it composed the control group. Confocal in vivo microscopy was performed before and after PHACO. Results. In patients with PEX after PE, an increase in number of nerve branches and pellet-like structures in them were noticed (p < 0,05).

Corneal epithelial dendritic cells in patients with multiple sclerosis: An in vivo confocal microscopy study

2020 ◽  
Vol 81 ◽  
pp. 139-143
Author(s):  
Valeria Testa ◽  
Nicole De Santis ◽  
Riccardo Scotto ◽  
Piero Della Giustina ◽  
Lorenzo Ferro Desideri ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Microscopy Study ◽  
In Vivo Confocal Microscopy ◽  
Corneal Epithelial

scholarly journals Alpha-Fetoprotein- and CD40Ligand-Expressing Dendritic Cells for Immunotherapy of Hepatocellular Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3375
Author(s):  
Annabelle Vogt ◽  
Farsaneh Sadeghlar ◽  
Tiyasha H. Ayub ◽  
Carlo Schneider ◽  
Christian Möhring ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
T Cells ◽  
Dendritic Cells ◽  
Tumor Regression ◽  
Combined Therapy ◽  
Term Survival ◽  
Effector Cells ◽  
Tumor Environment ◽  
The Impact

Dendritic cells (DC) as professional antigen presenting cells are able to prime T-cells against the tumor-associated antigen α-fetoprotein (AFP) for immunotherapy of hepatocellular carcinoma (HCC). However, a strong immunosuppressive tumor environment limits their efficacy in patients. The co-stimulation with CD40Ligand (CD40L) is critical in the maturation of DC and T-cell priming. In this study, the impact of intratumoral (i.t.) CD40L-expressing DC to improve vaccination with murine (m)AFP-transduced DC (Ad-mAFP-DC) was analyzed in subcutaneous (s.c.) and orthotopic murine HCC. Murine DC were adenovirally transduced with Ad-mAFP or Ad-CD40L. Hepa129-mAFP-cells were injected into the right flank or the liver of C3H-mice to induce subcutaneous (s.c.) and orthotopic HCC. For treatments, 106 Ad-mAFP-transduced DC were inoculated s.c. followed by 106 CD40L-expressing DC injected intratumorally (i.t.). S.c. inoculation with Ad-mAFP-transduced DC, as vaccine, induced a delay of tumor-growth of AFP-positive HCC compared to controls. When s.c.-inoculation of Ad-mAFP-DC was combined with i.t.-application of Ad-CD40L-DC synergistic antitumoral effects were observed and complete remissions and long-term survival in 62% of tumor-bearing animals were achieved. Analysis of the tumor environment at different time points revealed that s.c.-vaccination with Ad-mAFP-DC seems to stimulate tumor-specific effector cells, allowing an earlier recruitment of effector T-cells and a Th1 shift within the tumors. After i.t. co-stimulation with Ad-CD40L-DC, production of Th1-cytokines was strongly increased and accompanied by a robust tumor infiltration of mature DC, activated CD4+-, CD8+-T-cells as well as reduction of regulatory T-cells. Moreover, Ad-CD40L-DC induced tumor cell apoptosis. Intratumoral co-stimulation with CD40L-expressing DC significantly improves vaccination with Ad-mAFP-DC in pre-established HCC in vivo. Combined therapy caused an early and strong Th1-shift in the tumor environment as well as higher tumor apoptosis, leading to synergistic tumor regression of HCC. Thus, CD40L co-stimulation represents a promising tool for improving DC-based immunotherapy of HCC.

An in vivo confocal microscopy study of corneal changes in pseudoexfoliation syndrome

2018 ◽  
Vol 29 (5) ◽  
pp. 555-560 ◽  
Author(s):  
Luca Terracciano ◽  
Michela Cennamo ◽  
Eleonora Favuzza ◽  
Litasova Julia ◽  
Orsola Caporossi ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Anterior Segment ◽  
Senile Cataract ◽  
Nerve Plexus ◽  
Pseudoexfoliation Syndrome ◽  
Control Group ◽  
In Vivo Confocal Microscopy ◽  
Corneal Sensitivity ◽  
Corneal Layer

Purpose: To evaluate, through the in vivo confocal microscopy, the pathological changes of each corneal layer in eyes affected by pseudoexfoliation syndrome. Methods: We studied 40 eyes of 40 patients with diagnosis of unilateral senile cataract associated with pseudoexfoliation syndrome and 40 eyes of 40 control subjects with senile cataract without pseudoexfoliation syndrome. All patients underwent a complete ophthalmic examination including best corrected visual acuity, slit-lamp examination, corneal sensitivity measurement using a Cochet-Bonnet nylon thread esthesiometer, and anterior segment optical coherence tomography (Visante OCT, Carl Zeiss Meditec AG, Germany); in vivo confocal microscopy of corneal sections (endothelium, stroma, sub-basal nerve plexus, and superficial and basal epithelium) was performed with the ConfoScan 4.0 (Nidek, Japan). Results: In pseudoexfoliation syndrome group, the mean corneal sensitivity was 44.1 ± 1.3 mm and in the control group was 55.6 ± 4.7 mm. The corneas of the eyes with pseudoexfoliation syndrome were significantly less sensitive than those of control group eyes (p < 0.001). Pseudoexfoliation syndrome eyes had a lower nerve density and less nerve beadings and a higher degree of tortuosity in sub-basal plexus compared to the control group. The cell density of epithelial and endothelial layers was significantly lower in pseudoexfoliation syndrome eyes than controls. In 80% of pseudoexfoliation syndrome eyes, we found activated keratocytes and inflammatory cells in the anterior stroma. Conclusion: Our study demonstrates the morpho-structural corneal alterations in eyes affected by pseudoexfoliation syndrome, using corneal in vivo confocal microscopy as a non-invasive and high-reproducible technique to evaluate pathophysiology of each corneal layer; the sub-basal nerve plexus alterations are correlated with the lower corneal sensitivity.

scholarly journals Evaluation of Corneal Nerves and Dendritic Cells By in Vivo Confocal Microscopy After Descemet’s Membrane Keratoplasty For Bullous Keratopathy

2022 ◽  
Author(s):  
Takahiko Hayashi ◽  
Atsuyuki Ishida ◽  
Akira Kobayashi ◽  
Takefumi Yamaguchi ◽  
Nobuhisa Mizuki ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Bullous Keratopathy ◽  
Dependent Manner ◽  
In Vivo Confocal Microscopy ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Corneal Nerves ◽  
Corneal Nerve ◽  
Number Of Branches

Abstract This study evaluated changes in corneal nerves and the number of dendritic cells (DCs) in corneal basal epithelium following Descemet membrane endothelial keratoplasty (DMEK) surgery for bullous keratopathy (BK). Twenty-three eyes from 16 consecutive patients that underwent DMEK for BK were included. Eyes of age-matched patients that underwent pre-cataract surgery (12 eyes) were used as controls. In vivo confocal microscopy was performed pre- and postoperatively at 6, 12, and 24 months. Corneal nerve length, corneal nerve trunks, number of branches, and the number of DCs were determined. The total corneal nerve length of 1634.7 ± 1389.1 μm /mm2 before surgery was significantly increased in a time-dependent manner to 4485.8 ± 1403.7 μm /mm2, 6949.5 ± 1477.1 μm /mm2, and 9389.2 ± 2302.2 μm /mm2 at 6, 12, and 24 months after DMEK surgery, respectively. The DC density in BK cornea pre- and postoperatively at 6 months was significantly higher than in the controls, and decreased postoperatively at 12 and 24 months and was significantly lower than that at 6 months postoperatively. Thus, our results suggest that DMEK can repair and normalize the corneal environment.

scholarly journals A deep transfer learning framework for the automated assessment of corneal inflammation on in vivo confocal microscopy images

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252653
Author(s):  
Fan Xu ◽  
Yikun Qin ◽  
Wenjing He ◽  
Guangyi Huang ◽  
Jian Lv ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Transfer Learning ◽  
Inflammatory Cells ◽  
Computer Assisted ◽  
In Vivo Confocal Microscopy ◽  
Learning Models ◽  
Corneal Inflammation ◽  
Microscopy Images

Purpose Infiltration of activated dendritic cells and inflammatory cells in cornea represents an important marker for defining corneal inflammation. Deep transfer learning has presented a promising potential and is gaining more importance in computer assisted diagnosis. This study aimed to develop deep transfer learning models for automatic detection of activated dendritic cells and inflammatory cells using in vivo confocal microscopy images. Methods A total of 3453 images was used to train the models. External validation was performed on an independent test set of 558 images. A ground-truth label was assigned to each image by a panel of cornea specialists. We constructed a deep transfer learning network that consisted of a pre-trained network and an adaptation layer. In this work, five pre-trained networks were considered, namely VGG-16, ResNet-101, Inception V3, Xception, and Inception-ResNet V2. The performance of each transfer network was evaluated by calculating the area under the curve (AUC) of receiver operating characteristic, accuracy, sensitivity, specificity, and G mean. Results The best performance was achieved by Inception-ResNet V2 transfer model. In the validation set, the best transfer system achieved an AUC of 0.9646 (P<0.001) in identifying activated dendritic cells (accuracy, 0.9319; sensitivity, 0.8171; specificity, 0.9517; and G mean, 0.8872), and 0.9901 (P<0.001) in identifying inflammatory cells (accuracy, 0.9767; sensitivity, 0.9174; specificity, 0.9931; and G mean, 0.9545). Conclusions The deep transfer learning models provide a completely automated analysis of corneal inflammatory cellular components with high accuracy. The implementation of such models would greatly benefit the management of corneal diseases and reduce workloads for ophthalmologists.

scholarly journals In vivo confocal microscopy features and clinicohistological correlation of limbal nerve corpuscles

2019 ◽  
pp. bjophthalmol-2018-313199 ◽  
Author(s):  
Mouhamed Ali Al-Aqaba ◽  
Fady S Anis ◽  
Imran Mohammed ◽  
Anjali Dias Samarawickrama Yapa ◽  
Winfried M Amoaku ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Digital Pathology ◽  
In Vivo Confocal Microscopy ◽  
Eye Bank ◽  
Histological Features ◽  
Corneal Nerves ◽  
Normal Human ◽  
Epithelial Layers ◽  
Whole Mounts

AimsTo describe the in vivo confocal microscopy (IVCM) features of human limbal nerve corpuscles (LNCs) and correlate these with the histological features.MethodsWe examined 40 eyes of 29 healthy living subjects (17 female, 12 male; mean age=47.6) by IVCM. Four limbal quadrants were scanned through all epithelial layers and stroma to identify the LNCs and associated nerves. Ten fresh normal human corneoscleral discs from five deceased patients with a mean age of 67 years and 17 eye-bank corneoscleral rims with a mean age of 57.6 years were stained as whole mounts by the acetylcholinesterase (AChE) method to demonstrate LNCs and corneal nerves. Stained tissue was scanned in multiple layers with the NanoZoomer digital pathology microscope. The in vivo results were correlated to the histological findings.ResultsOn IVCM, LNCs were identified in 65% of the eyes studied and were mainly (84%) located in the inferior or superior limbal regions. They appeared either as bright (hyper-reflective) round or oval single structures within the hyporeflective, relatively acellular fibrous core of the palisades or were clustered in groups, often located anterior to the palisades of Vogt. They measured 36 µm in largest diameter (range 20–56 µm). The in vivo features were consistent with the histology, which showed LNCs as strongly AChE positive round or oval structures.ConclusionThe strong correlation with histology will enable use of IVCM to study LNCs in normal and disease conditions.

scholarly journals In Vivo Confocal Microscopy of Cornea in Patients with Terrien’s Marginal Corneal Degeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ting Chen ◽  
Qiangxiang Li ◽  
Xiangbo Tang ◽  
Min Liao ◽  
Hua Wang
Keyword(s):  
Epithelial Cells ◽  
Confocal Microscopy ◽  
Stromal Cells ◽  
Nerve Fibers ◽  
In Vivo Confocal Microscopy ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Department Of Ophthalmology ◽  
Severity Of The Disease

This study was aimed at observing the morphological changes of the cornea with ocular in vivo confocal microscopy (IVCM) in patients with Terrien’s marginal degeneration (TMD). Ten patients (20 eyes) with TMD treated in the Department of Ophthalmology, Xiangya Hospital, and 10 healthy controls (20 eyes) were included in the current study. A detailed slit lamp microscopy, anterior segment photography, and corneal IVCM examination were performed for each eye. The density of central and marginal corneal epithelial cells, stromal cells, and subepithelial nerve fibers was compared between the two groups using the Wilcoxon rank sum test. Compared with the control group, the corneal epithelial and endothelial cells in the TMD group showed granular highly reflective substances and thinner subepithelial nerve fibers. The uneven dot-like highly reflective substances without cell structures appeared in the stromal layer of the cornea. The density of central and marginal corneal epithelial cells, stromal cells, and subepithelial nerve fibers was lower in the TMD group (p<0.05), and they were negatively correlated with severity of the disease (p<0.05). Our study demonstrated that the density of corneal epithelial cells, stromal cells, and sensory plexus nerve fibers was significantly reduced in the TMD group. The pathological changes were more obvious in the marginal cornea, and it is correlated with severity of the disease.

scholarly journals In Vivo Distribution of Corneal Epithelial Dendritic Cells in Patients With Glaucoma

2016 ◽  
Vol 57 (14) ◽  
pp. 5996 ◽  
Author(s):  
Rodolfo Mastropasqua ◽  
Luca Agnifili ◽  
Vincenzo Fasanella ◽  
Andrea Lappa ◽  
Lorenza Brescia ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Corneal Epithelial ◽  
In Vivo Distribution
Sign in / Sign up

Export Citation Format

Share Document