scholarly journals Evaluation of sensitivity to chemical disinfectants of mycobacterium tuberculosis strains isolated from HIV-infected individuals and patients with monotuberculosis infection compared to the reference strain

2021 ◽  
Vol 38 (2) ◽  
pp. 120-125
Author(s):  
V. I. Sergevnin ◽  
O. V. Tukacheva ◽  
О. R. Sibiryakova ◽  
E. V. Sarmometov ◽  
P. B. Azanov
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Quaternary Ammonium ◽  
Culture Medium ◽  
Reference Strain ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound ◽  
Control Strain

Objective. To study the sensitivity to chemical disinfectants against Mycobacterium tuberculosis isolated from HIV-infected individuals and patients with monotuberculosis in comparison to the reference strain of Mycobacterium terrae. Materials and methods. The sensitivity of 12 M. tuberculosis strains isolated from patients with HIV-associated tuberculosis (n = 6) and monotuberculosis (n = 6) to chlorine-containing and quaternary ammonium compound (QAC) disinfectants was studied. The M. terrae strain was used as a reference. Seedings of microbial suspensions treated with disinfectants on the culture medium Levenshtein-Jensen were carried out and were monitored for 3 months. Results. One, two, three months following the treatment of microorganisms with QAC-containing disinfectant in the absence of growth of the control strain, there was noted growth of M. tuberculosis, particularly intense in the strains isolated from HIV-infected persons. Conclusions. Thus, the resistance of tuberculosis pathogens to disinfectants can be acquired. M. tuberculosis strains isolated from patients with HIV-associated tuberculosis are detected to be more resistant to disinfectants than the strains isolated from patients with monotubercular infection.

scholarly journals Prevalence and Detection of qac Genes from Disinfectant-Resistant Staphylococcus aureus Isolated from Salon Tools in Ishaka Town, Bushenyi District of Uganda

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Solange Gahongayire ◽  
Adamu Almustapha Aliero ◽  
Charles Drago Kato ◽  
Alice Namatovu
Keyword(s):  
Sodium Hypochlorite ◽  
Quaternary Ammonium ◽  
Bacterial Infections ◽  
Healthcare Providers ◽  
Quaternary Ammonium Compound ◽  
Diffusion Method ◽  
Ammonium Compound ◽  
Bacterial Strains ◽  
Microbiological Methods ◽  
Phenotypic Methods

Bacterial infections are on a rise with causal-resistant strains increasing the economic burden to both patients and healthcare providers. Salons are recently reported as one of the sources for transmission of such resistant bacterial strains. The current study aimed at the identification of the prevalent bacteria and characterization of quaternary ammonium compound (qac) genes from disinfectant-resistant S. aureus isolated from salon tools in Ishaka town, Bushenyi District of Uganda. A total of 125 swabs were collected from different salon tools (combs, brushes, scissors, clippers, and shaving machines), and prevalent bacteria were isolated using standard microbiological methods. Identification of isolated bacteria was done using standard phenotypic methods including analytical profile index (API). Susceptibility patterns of the isolated bacteria to disinfectant were determined using the agar well diffusion method. Quaternary ammonium compound (qac) genes (qacA/B and qacC) associated with disinfectant resistances were detected from disinfectant-resistant S. aureus using multiplex polymerase chain reaction (PCR) and Sanger sequencing methods. Of the 125 swab samples collected from salons, 78 (62.4%) were contaminated with different bacteria species. Among the salon tools, clippers had the highest contamination of 20 (80.0%), while shaving machines had the lowest contamination of 11 (44.0%). The most prevalent bacteria identified were Staphylococcus epidermidis (28.1%) followed by S. aureus (26.5%). Of all the disinfectants tested, the highest resistance was shown with sodium hypochlorite 1%. Out of the eight (8) disinfectant-resistant S. aureus analysed for qac genes, 2 (25%) isolates (STP6 and STP9) were found to be qacA/B positive, while 2 (25%) isolates (STP8 and STP9) were found to be qacC gene positive. This study has shown that bacterial contamination of salon tools is common, coupled with resistance to disinfectants with sodium hypochlorite resistance being more common. Furthermore, observed resistance was attributed to the presence of qac genes among S. aureus isolates. A search for qac genes for disinfectant resistance from other bacteria species is recommended.

Building a Better Quaternary Ammonium Compound (QAC): Branched Tetracationic Antiseptic Amphiphiles

ChemMedChem ◽  
2016 ◽  
Vol 11 (13) ◽  
pp. 1401-1405 ◽  
Author(s):  
Megan E. Forman ◽  
Megan C. Jennings ◽  
William M. Wuest ◽  
Kevin P. C. Minbiole
Keyword(s):  
Quaternary Ammonium ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound
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