scholarly journals Hubungan Kadar Progesteron pada Fase Awal Luteal dengan Kematian Embrio pada Sapi Aceh

2019 ◽  
Vol 19 (2) ◽  
pp. 107-112
Author(s):  
Budianto Panjaitan ◽  
Citra Chyntia Helwana ◽  
Nellita Meutia ◽  
Yusmadi Yusmadi ◽  
Tongku Nizwan Siregar ◽  
...  
Keyword(s):  
Body Weight ◽  
Corpus Luteum ◽  
Adult Female ◽  
Artificial Insemination ◽  
Luteal Phase ◽  
Embryonic Mortality ◽  
Progesterone Concentration ◽  
Double Injection ◽  
Progesterone Secretion ◽  
Embryonic Death

ABSTRAK.  Progesteron merupakan hormon yang berperan penting dalam proses pemeliharaan kebuntingan dan dihasilkan oleh corpus luteum. Penelitian ini bertujuan mengetahui hubungan antara kadar hormon progesteron pada fase awal luteal dengan kematian embrio pada sapi Aceh. Dalam penelitian ini digunakan empat ekor sapi betina dewasa berumur 3-5 tahun, bobot badan 150-250 kg, sehat secara klinis, dan memiliki reproduksi normal. Sapi disinkronisasi menggunakan 5 ml prostaglandin F2 alfa (PGF2α) dengan pola penyuntikan ganda berinterval 11 hari. Koleksi sampel darah untuk pengukuran konsentrasi progesteron dilakukan pada hari ke-5, 6, dan 7 pasca inseminasi. Pengukuran konsentrasi progesteron dilakukan menggunakan metode enzymelinked-immunoassay (ELISA), pemeriksaan kebuntingan dan kematian embrio menggunakan metode transrektal ultrasonografi pada hari ke-25 pasca inseminasi. Pemeriksaan diulang setiap 10 hari sampai hari ke-55 pasca inseminasi. Puncak sekresi progesteron pada sapi bunting dengan embrio yang bertahan hidup terdapat pada hari ke-7 (2,082 ng/ml), pada sapi Late Embryonic Mortality (LEM) di hari ke-5 (8,209 ng/ml) dan pada sapi tidak bunting di hari ke-7 (3,051±1,157 ng/ml). Sekresi progesteron sapi LEM pada hari ke-5 sampai dengan ke-7 cenderung menurun sedangkan pada sapi yang bertahan hidup cenderung meningkat.  (Correlation between progesterone levels in early luteal phase and embryonic death  in Aceh cattle) ABSTRACT. Progesterone is an important hormone that functions to maintain pregnancy and is produced by the corpus luteum. The aim of this study was to see a correlation between progesterone and the incidence of embryonic death in Aceh cattle. This study used four adult female cows, 3-5 years old, 150-250 kg body weight, clinically healthy, and have a normal reproduction. The synchronized with 5 ml prostaglandin F2 alfa hormone, and double injection pattern with 11-day intervals. The blood was collected for progesterone measurements on 5th, 6th, 7th day post artificial insemination. Measurement of progesterone concentration was carried out using an enzymelinked-immunoassay (ELISA), while pregnancy and embryo mortality was performed using the trans-rectal ultrasonography method on the 25th day after insemination. The examination was repeated every 10 days until day 55th after insemination. Progesterone secretion peaks in pregnant cows were on day 7th (2.082 ng/ml), in cattle Late Embryonic Mortality (LEM) on day 5th (8.209 ng/ml) and in cattle not pregnant on day 7th (3.051±1.157 ng/ml). The pattern of LEM progesterone secretion on days 5th to 7th tends to decrease while those that survive tend to increase.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

scholarly journals Delayed effect of low progesterone concentrations on bovine uterine PGF(2alpha) secretion in the subsequent oestrous cycle

Reproduction ◽  
2001 ◽  
pp. 643-648 ◽  
Author(s):  
A Shaham-Albalancy ◽  
Y Folman ◽  
M Kaim ◽  
M Rosenberg ◽  
D Wolfenson
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Oestrous Cycle ◽  
Delayed Effect ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Progesterone Treatment ◽  
Late Luteal Phase ◽  
Subsequent Cycle ◽  
Concentration Curves

Low progesterone concentrations during the bovine oestrous cycle induce enhanced responsiveness to oxytocin challenge late in the luteal phase of the same cycle. The delayed effect of low progesterone concentrations during one oestrous cycle on uterine PGF(2alpha) secretion after oxytocin challenge on day 15 or 16 of the subsequent cycle was studied by measuring the concentrations of the major PGF(2alpha) metabolite (13,14-dihydro-15-keto PGF(2alpha); PGFM) in plasma. Two experiments were conducted, differing in the type of progesterone treatment and in the shape of the low progesterone concentration curves. In Expt 1, progesterone supplementation with intravaginal progesterone inserts, with or without an active corpus luteum, was used to obtain high, or low and constant plasma progesterone concentrations, respectively. In Expt 2, untreated cows, representing high progesterone treatment, were compared with cows that had low but increasing plasma progesterone concentrations that were achieved by manipulating endogenous progesterone secretion of the corpus luteum. Neither experiment revealed any differences in plasma progesterone concentrations between the high and low progesterone groups in the subsequent oestrous cycle. In both experiments, both groups had similar basal concentrations of PGFM on day 15 (Expt 1) or 16 (Expt 2) of the subsequent oestrous cycle, 18 days after progesterone treatments had ended. In both experiments, the increases in PGFM concentrations in the low progesterone groups after an oxytocin challenge were markedly higher than in the high progesterone groups. These results indicate that low progesterone concentrations during an oestrous cycle have a delayed stimulatory effect on uterine responsiveness to oxytocin during the late luteal phase of the subsequent cycle. This resulting increase in PGF(2alpha) secretion may interfere with luteal maintenance during the early stages of pregnancy.

CONCENTRATION OF PROSTAGLANDIN F2α AND STEROIDS IN THE HUMAN CORPUS LUTEUM

1977 ◽  
Vol 73 (1) ◽  
pp. 115-122 ◽  
Author(s):  
I. A. SWANSTON ◽  
K. P. McNATTY ◽  
D. T. BAIRD
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Progesterone Concentration ◽  
Late Luteal Phase ◽  
Prostaglandin F ◽  
Human Corpus Luteum

SUMMARY The concentration of prostaglandin F2α (PGF2α), progesterone, pregnenolone, oestradiol-17β, oestrone, androstenedione and testosterone was measured in corpora lutea obtained from 40 women at various stages of the menstrual cycle. The concentration of PGF2α was significantly higher in corpora lutea immediately after ovulation (26·7 ± 3·9 (s.e.m.) ng/g, P < 0·005) and in corpora albicantia (16·3 ± 3·3 ng/g, P < 0·005) than at any other time during the luteal phase. There was no correlation between the concentration of PGF2α and that of any steroid. The progesterone concentration was highest in corpora lutea just after ovulation (24·9 ± 6·7 μg/g) and in early luteal groups (25·7 ± 6·8 μg/g) but declined significantly (P < 0·05) to its lowest level in corpora albicantia (1·82 ± 0·66 μg/g). The concentration of oestradiol-17β in the corpus luteum and luteal weight were significantly greater during the mid-luteal phase than at any other stage (concentration 282 ± 43 ng/g, P < 0·05; weight 1·86 ± 0·18 g, P < 0·005). The results indicate that regression of the human corpus luteum is not caused by a rise in the ovarian concentration of PGF2α in the late luteal phase of the cycle.

OVARIAN PROGESTERONE SECRETION DURING THE OESTROUS CYCLE, PREGNANCY AND LACTATION IN DOGS

1963 ◽  
Vol 44 (3) ◽  
pp. 461-466 ◽  
Author(s):  
G. Telegdy ◽  
E. Endröczi ◽  
K. Lissák
Keyword(s):  
Body Weight ◽  
Corpus Luteum ◽  
Human Placenta ◽  
Early Stage ◽  
Venous Blood ◽  
Placental Tissue ◽  
Oestrous Cycle ◽  
Gradual Decrease ◽  
Progesterone Secretion ◽  
Pregnancy And Lactation

ABSTRACT The concentration of progesterone in the ovarian venous blood of female dogs varied between 0.13 and 2.70 μg/h per ovary and per kg body weight, in the different phases of the oestrous cycle. There was no evidence of progesterone secretion in the absence of the corpus luteum. The highest values of progesterone secretion were detected in the early stage of pregnancy (12.9-16.2 μg/h per ovary and per kg body weight). In the course of pregnancy, a gradual decrease in progesterone secretion was found which fell below detectable amounts 48 hours after parturition. The ovarian venous blood did not contain any progesterone in the presence of a corpus luteum of lactation. There was some progesterone in the extracts of placental tissue, however, though in much smaller quantities than in the human placenta.

scholarly journals Luteal phase deficiency: pathophysiology and role in reproductive disorders

2021 ◽  
pp. 89-97
Author(s):  
I. A. Ivanov
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Pregnancy Loss ◽  
Embryo Implantation ◽  
Endometrial Biopsy ◽  
Reproductive Disorders ◽  
Normal Functioning ◽  
Art Programs ◽  
Progesterone Secretion ◽  
Lh Secretion

It is well known that corpus luteum normal functioning is crucial for the luteal phase, which determines the embryo implantation and the progression of pregnancy. Luteal phase deficiency (LPD), associated with impaired progesterone secretion by the corpus luteum, is considered as a significant factor of infertility and early pregnancy loss, both in the natural cycle and in assisted reproductive technology (ART) programs. The LPD formation is associated with hypothalamic-pituitary-ovarian axis dysregulation, which leads to abnormal secretion of FSH, LH, ovulation and luteinization disorders, premature luteolysis. The most significant problem in the study of LPD is the lack of reliable and reproducible methods of its verification. This review summarizes the available data on the methods and issues of LPD diagnosing, including the duration of the luteal phase, the level of progesterone secretion, and endometrial biopsy. LPD is an important factor in reproductive failures during IVF, which is caused by suppression of the physiological FSH, LH secretion and requires mandatory progesterone support in the luteal phase of the cycle. It’s hard to define the contribution of LPD to miscarriage, however, empirical progestogen therapy may increase the live births rate in women with recurrent pregnancy loss. Currently, there is no evidence of the LPD role and progesterone support effectiveness in infertility management, so the diagnosis and therapy of LPD among these patients should not be considered.

scholarly journals Progesterone concentration in the marsupial Sminthopsis macroura: relationship with the conceptus, uterine glandular regeneration and body weight

Reproduction ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 107-117 ◽  
Author(s):  
E M Menkhorst ◽  
L A Hinds ◽  
L Selwood
Keyword(s):  
Body Weight ◽  
Mitotic Activity ◽  
Luteal Phase ◽  
Gestation Length ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Body Weights ◽  
Sminthopsis Macroura ◽  
Cell Arrest ◽  
First Time

Close examination of hormonal profiles and uterine morphology in the marsupial reproductive cycle highlights significant differences between pregnant and non-pregnant cycles. In the polyovular dasyurid marsupial Sminthopsis macroura, we identified changes associated with gestation by comparing ovarian and plasma progesterone concentrations, uterine weights, uterine epithelial mitoses, body weights and gestation lengths between pregnant and non-pregnant luteal phases. The plasma progesterone profile of S. macroura was biphasic, peaking during unilaminar blastocyst expansion and on the day of implantation. Periods of rapid embryonic development were associated with increasing plasma progesterone concentrations and animal body weight. For the first time in a polyovular marsupial, we identified 1) a correlation between ovarian progesterone concentration and conceptus number during the luteal phase just prior to implantation (total ovarian progesterone), indicating a conceptus influence on progesterone concentration; 2) a pulse of uterine epithelial mitotic activity at the time of implantation and 3) increased mitotic activity in pregnant animals during unilaminar blastocyst formation compared with non-pregnant animals. Gestation length was reduced by up to 15%, due to the loss of, or reduction in, the four-cell arrest and more rapid definitive blastocyst expansion. This is the first time a conceptus influence on gestation length has been identified in a dasyurid. This study provides further evidence for the modification of the luteal phase by pregnancy in S. macroura.

SUPPRESSION OF LUTEAL PROGESTERONE SECRETION IN THE STUMPTAILED MACAQUE BY AN ANTAGONIST ANALOGUE OF LUTEINIZING HORMONE RELEASING HORMONE.

1985 ◽  
Vol 104 (2) ◽  
pp. R1-R4 ◽  
Author(s):  
H.M. Fraser ◽  
D.T. Baird ◽  
G.I. McRae ◽  
J.J. Nestor ◽  
B.H. Vickery
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Releasing Hormone ◽  
Expected Time ◽  
Progesterone Secretion ◽  
Serum Lh ◽  
Late Luteal Phase ◽  
Serum Hormone ◽  
Lhrh Antagonist ◽  
Lh Releasing Hormone

ABSTRACT The dependence of progesterone secretion from the corpus luteum on pituitary gonadotrophin was examined in the cyclic stumptailed macaque by studying the effects of a single s.c. injection of a potent LH releasing hormone (LHRH) antagonist, [N–Ac–D–Nal(2)1, D–pCl–phe2, D–Trp3, D–hArg(Et2)6, D–Ala10] LHRH. A dose of 100 μg antagonist/kg administered on days 9/10 of the luteal phase in three monkeys caused a marked temporary suppression of serum concentrations of LH and progesterone during the following 32 h but levels still remained detectable and after 2 days serum hormone concentrations returned to the normal luteal-phase range. When the same animals were treated with 300 μg antagonist/kg at the same period during a subsequent cycle, serum LH levels were close to or at the limits of detection of the bioassay for the next 48 h and progesterone concentrations declined steadily, reaching non-detectable values by 48 h. In two monkeys the progesterone levels remained suppressed and they menstruated prematurely; in the third monkey the progesterone concentration rose to just above baseline and menstruation occurred at the expected time. Administration of 300μg antagonist/ kg on days 6/7 of the luteal phase in a further three monkeys also suppressed progesterone concentrations but not to baseline values, and after 2 days a normal progesterone profile was regained. These results suggest that the corpus luteum of the stumptailed macaque is largely dependent on pituitary gonadotrophin support during the mid to late luteal phase.

Developement, function and regression of the corpus luteum in the marsupial Trichosurus vulpecula

1973 ◽  
Vol 21 (4) ◽  
pp. 477 ◽  
Author(s):  
CD Shorey ◽  
RL Hughes
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Secretory Activity ◽  
Oestrous Cycle ◽  
Secretion Rate ◽  
Trichosurus Vulpecula ◽  
Progesterone Concentration ◽  
Apparent Increase ◽  
Pregnant Females ◽  
Secretion Rates

Following ovulation, which usually takes place on day 1 of the 26-day oestrous cycle in T. culpecula, a single corpus luteum begins to develop. During the period before the uterine luteal phase begins on day 8 of the cycle, the granulosa lutein cells are active in the synthesis of what appears to be a cholesterol-like substance at a time when the ovarian secretion rates of progesterone are re!atively low. The uterine luteal phase extends from day 8 to day 15 of the cycle. During this phase the ovarian secretion rates of progesterone are relatively high, and reach maximal levels on days 12-1 3. This high secretory activity is manifested in the granulosa lutein cells by an apparent increase in the number of mitochondria, thought to be involved with the conversion of intracellularly stored cholesterol to pregnenolone, and the development of whorl-like configurations of agranular reticulum which are reported to be concerned with the conversion of pregnenolone to progesterone. A drop in progesterone concentration commences in pregnant and non-pregnant females on day 17 of the cycle, when early involution of the corpus luteunl is apparent. By day 20, the corpus luteum regresses to an early corpus albicans, and the ovarian secretion rate of progesterone falls progressively to levels similar to those in anoestrous females.

Local progesterone secretion by the marmoset corpus luteum after perfusion with regulating hormones

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S111-S112 ◽  
Author(s):  
G.E. WEBLEY ◽  
J.P. HEARN ◽  
M.R. LUCK
Keyword(s):  
Corpus Luteum ◽  
Progesterone Secretion
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