IN-BUILT ANTIOXIDATION CAPACITY OF sub1A QTL IN RICE (Oryza sativa L.) AND ITS MODULATION BY EXOGENOUS APPLICATION OF POLYAMINE AND NITRIC OXIDE

Arsenic (As) is a highly toxic metalloid for all forms of life including plants. Rice is the main food source for different countries worldwide, although it can take up high amounts of As in comparison with other crops, showing toxic profiles such as decreases in plant growth and yield. The induction of oxidative stress is the main process underlying arsenic toxicity in plants, including rice, due to an alteration of the reactive oxygen species (ROS) metabolism. The aim of this work was to gain better knowledge on how the ROS metabolism and its interaction with nitric oxide (NO) operate under As stress conditions in rice plants. Thus, physiological and ROS-related biochemical parameters in roots and shoots from rice (Oryza sativa L.) were studied under 50 μM arsenate (AsV) stress, and the involvement of the main antioxidative systems and NO in the response of plants to those conditions was investigated. A decrease of 51% in root length and 27% in plant biomass was observed with 50 μM AsV treatment, as compared to control plants. The results of the activity of superoxide dismutase (SOD) isozymes, catalase, peroxidase (POD: total and isoenzymatic), and the enzymes of the ascorbate–glutathione cycle, besides the ascorbate and glutathione contents, showed that As accumulation provoked an overall significant increase of most of them, but with different profiles depending on the plant organ, either root or shoot. Among the seven identified POD isozymes, the induction of the POD-3 in shoots under As stress could help to maintain the hydrogen peroxide (H2O2) redox homeostasis and compensate the loss of the ascorbate peroxidase (APX) activity in both roots and shoots. Lipid peroxidation was slightly increased in roots and shoots from As-treated plants. The H2O2 and NO contents were enhanced in roots and shoots against arsenic stress. In spite of the increase of most antioxidative systems, a mild oxidative stress situation appears to be consolidated overall, since the growth parameters and those from the oxidative damage could not be totally counteracted. In these conditions, the higher levels of H2O2 and NO suggest that signaling events are simultaneously occurring in the whole plant.

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S-nitrosocysteine-responsive genes modulate diverse regulatory pathways in Oryza sativa: a transcriptome profiling study

Functional Plant Biology ◽

10.1071/fp17249 ◽

2018 ◽

Vol 45 (6) ◽

pp. 630 ◽

Cited By ~ 8

Author(s):

Bong-Gyu Mun ◽

Sang-Uk Lee ◽

Adil Hussain ◽

Hyun-Ho Kim ◽

Nkulu Kabange Rolly ◽

...

Keyword(s):

Nitric Oxide ◽

Oryza Sativa ◽

Transcriptional Control ◽

Nitrosative Stress ◽

Genetic Model ◽

Oryza Sativa L ◽

Expression Patterns ◽

Nitric Oxide Donor ◽

Transcriptional Networks ◽

Regulatory Pathways

Rice (Oryza sativa L.) is a major food crop and also a well-established genetic model. Nitric oxide (NO) and its derivatives are important signalling molecules that actively participate in various signalling pathways in response to different stresses. In this study, we performed RNA-seq mediated transcriptomic analysis of rice after treatment with the nitric oxide donor, S-nitroso-L-cysteine (CySNO), generating an average of 37.5 and 41.5 million reads from control and treated leaf samples respectively. More than 95% of the reads were successfully mapped to the O. sativa reference genome yielding a total of 33 539 differentially expressed genes (DEGs, P < 0.05). Further analyses identified 825 genes with at least 2-fold change in the expression following treatment with CySNO (P < 0.01). The DEGs identified were involved in diverse molecular functions such as catalytic activity, binding, transport, and receptor activity and were mostly located in the membrane, organelles such as nucleus, Golgi apparatus and mitochondria. DEGs also contained several genes that regulate responses to abiotic stresses such as drought, heat, cold and salt stress and biotic stresses. We also found significantly similar expression patterns of CySNO-responsive DEGs of rice with the CySNO-responsive DEGs of Arabidopsis in a previous study. Expression patterns of genes involved in key biological functions were verified using quantitative real time (qRT)-PCR. The findings of this study suggest that NO regulates the transcriptional control of genes involved in a wide variety of physiological functions in rice, and that NO-mediated transcriptional networks are highly conserved across the plant kingdom. This study provides useful information regarding the transcriptional response of plants to nitrosative stress.

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Exogenous application of rutin and gallic acid regulate antioxidants and alleviate reactive oxygen generation in Oryza sativa L.

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-017-0430-2 ◽

2017 ◽

Vol 23 (2) ◽

pp. 301-309 ◽

Cited By ~ 7

Author(s):

Akanksha Singh ◽

Rupali Gupta ◽

Rakesh Pandey

Keyword(s):

Oryza Sativa ◽

Gallic Acid ◽

Oryza Sativa L ◽

Reactive Oxygen ◽

Oxygen Generation ◽

Exogenous Application

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Exogenous application of spermidine (SPD) and wood vinegar improves salt tolerance in salt-sensitive rice (Oryza sativa L.)

Pakistan Journal of Botany ◽

10.30848/pjb2021-1(42) ◽

2020 ◽

Vol 53 (1) ◽

Author(s):

Piyada Theerakulpisut ◽

Punya Madee ◽

Dechudom Pamuta ◽

Noppawan Nounjan

Keyword(s):

Oryza Sativa ◽

Salt Tolerance ◽

Oryza Sativa L ◽

Exogenous Application ◽

Wood Vinegar

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Insights into nitric oxide-mediated water balance, antioxidant defence and mineral homeostasis in rice (Oryza sativa L.) under chilling stress

Nitric Oxide ◽

10.1016/j.niox.2020.04.001 ◽

2020 ◽

Vol 100-101 ◽

pp. 7-16 ◽

Cited By ~ 8

Author(s):

Abdullah Al Mamun Sohag ◽

Md Tahjib-Ul-Arif ◽

Sonya Afrin ◽

Md Kawsar Khan ◽

Md Abdul Hannan ◽

...

Keyword(s):

Nitric Oxide ◽

Oryza Sativa ◽

Water Balance ◽

Oryza Sativa L ◽

Chilling Stress ◽

Antioxidant Defence ◽

Mineral Homeostasis

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Improving salinity tolerance in transplanted aman rice (Oryza sativa L.) by exogenous application of proline

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v17i2.41943 ◽

2019 ◽

Vol 17 (2) ◽

pp. 194-199

Author(s):

Noor -Us- Sane ◽

Dhiman Bhusan ◽

Papon Kumar Deb Nath ◽

Yoshiyuki Murata ◽

Md. Anamul Hoque

Keyword(s):

Oryza Sativa ◽

Nutrient Uptake ◽

Oryza Sativa L ◽

Seedling Stage ◽

Growth And Yield ◽

Yield Potential ◽

Vegetative Stage ◽

Silty Clay ◽

Exogenous Application ◽

Experimental Plots

Salinity is the major factor reducing crop yield in coastal areas of Bangladesh. Proline (Pro) application with suitable crop varieties having higher yield potential could contribute to the improvement of crop production in saline areas. The main objective of this study was to investigate the mitigation of adverse effects of salinity in aman rice by exogenously applied Pro. The experiment was carried out at the farmer’s field of Batiaghata, Khulna. Characteristically, the soil was silty clay loam having pH 6.7, EC 4.6 dS m–1, CEC 23 meq/100 g soil, organic matter 0.71%. Rice (Oryza sativa L.) variety BR23 was used as plant material. The experiment was laid out in a randomized complete block design with three replications. There were different treatment combinations namely control (no Pro), 25 mM Pro at seedling stage, 25 mM Pro at vegetative stage, 25 mM Pro at seedling and vegetative stages, 50 mM Pro at seedling stage, 50 mM Pro at vegetative stage, 50 mM Pro at seedling and vegetative stages, 100 mM Pro at seedling stage, 100 mM Pro at vegetative stages, and 100 mM Pro at seedling and vegetative stages. Recommended doses of N, P, K, S and Zn fertilizers were applied to the all experimental plots. Thirty-day-old seedlings were transplanted in the experimental plots. Proline solutions were sprayed over plant leaves with the help of sprayer as per treatments. Salinity caused significant reductions in growth and yield of BR23 by decreasing plant height, number of effective tillers, panicle length, filled grains panicle-1 and 1000-grain weight. On the other hand, exogenous application of Pro showed a significant increase in growth and yield of BR23 under saline conditions. Results also revealed that growth and yield of rice did not increase proportionally with the increasing doses of Pro. Proline application resulted in significant increases in K+/Na+ and nutrient uptake by rice under salinity. The present study suggests that exogenous application of Pro confers tolerance to salinity in aman rice by increasing K+/Na+ ratio and nutrient uptake. J. Bangladesh Agril. Univ. 17(2): 194–199, June 2019

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