Dynamics of changes in type 1 and 2 insulinlike growth factors in the blood of patients with oral squamous cell cancer after targeted therapy with cetuximab

Objective. Studying the blood levels of type 1 and 2 insulin-like growth factors in patients with squamous cell carcinoma of the tongue and mouth floor mucosa depending on the therapy effect.Materials and Methods. The study included data from 30 patients with squamous cell carcinoma of the tongue and mouth floor mucosa T3–4N0–1M0 who received chemotherapy cycles together with targeted therapy with cetuximab. Twenty non-cancer donors were examined as well. Depending on the therapy effect, patients were divided into two groups: sensitive and resistant ones.Results. Initial levels of IGF-1 and IGF-2 in the blood serum of patients prior to chemotherapy and targeted therapy with cetuximab were lower than the levels in donors by 53.5 % and 20.3 %, respectively. After chemotherapy and cetuximab therapy, patients with sensitivity to the treatment showed normalization of IGF-1 and its significant increase compared to the initial levels — by 87 %. Levels of IGF-2 were not statistically significantly different from the initial levels and were 32.5 % lower than in donors. The IGF-1 / IGF-2 coefficient was 58 % higher than the initial value.Conclusions. Chemotherapy and cetuximab therapy normalized levels of IGF-1 in patients with sensitivity to the treatment which was demonstrated by an increase in IGF-1 up to the normal blood levels in effective treatment.

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STUDY OF VEGF-A AND TGF-Β LEVELS IN BIOPTATES OF SQUAMOUS CELL CARCINOMA OF THE TONGUE AND MOUTH FLOOR MUCOSA IN POLYCHEMOTHERAPY WITH MONOCLONAL ANTIBODIES – CETUXIMAB

Research n Practical Medicine Journal ◽

10.17709/2409-2231-2019-6-3-2 ◽

2019 ◽

Vol 6 (3) ◽

pp. 20-28 ◽

Cited By ~ 1

Author(s):

O. I. Kit ◽

E. M. Frantsiyants ◽

I. V. Neskubina ◽

L. Yu. Vladimirova ◽

A. A. Lyanova ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Growth Factors ◽

Targeted Therapy ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tumor Tissue ◽

Statistical Processing ◽

Antitumor Therapy ◽

Initial Values ◽

Carcinoma Of The Tongue

Aim. Study of VEGF-A and TGF-β growth factors in tumor tissue bioptates in patients with squamous cell carcinoma of the tongue and floor of the oral cavity depending on the effectiveness of polychemotherapy targeted therapy with cetuximab. Patients and methods. The study included 30 patients with squamous cell carcinoma of the tongue and mouth floor mucosa (T3-4N0-1M0). All patients received 2 cycles of therapy: cisplatin 100 mg/m2 , intravenously, day 1, 5-fluorouracil 1000 mg/m2 /day, intravenously, 96-hour continuous infusion in combination with targeted therapy (cetuximab 400 mg/ m2 on day 1 in a loading dose, then 250 mg/m2 on days 8 and 15). Patients were divided into two subgroups: target sensitivity of patients (partial regression and stabilization) n = 17 and target resistance (progression) n = 13. Levels of growth factors VEGF-A and TGF-β were determined in tumor tissue bioptates by ELISA using standard test systems (Bender Med System, Austria). Statistical processing of results was performed using the Statistica 6.0 program (Stat-Soft, 2001). Results. Polychemotherapy with cetuximab in some patients (n = 13), antitumor therapy with cetuximab did not result in statistically significant changes in levels of VEGF-A, TGF-β and the VEGF-A/TGF-β ratio compared to the initial values. In other patients (n = 17), the studied markers in tumor tissue bioptates were statistically significantly different from the initial values: VEGF-A was decreased by 1.46 times, TGF-β by 2.96 times, while the VEGF-A/TGF-β ratio was twice elevated. Conclusions. The results on levels of growth factors VEGF-A and TGF-β, as well as the VEGF-A/TGF-β ratio, are of a certain prognostic value and can be used as criteria for evaluating the efficacy of antitumor therapy in patients with squamous cell carcinoma of the tongue and mouth floor mucosa.

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STUDY OF INSULIN-LIKE GROWTH FACTORS IN THE BLOOD OF PATIENTS WITH SQUAMOUS CELL CARCINOMA OF THE TONGUE AND LOWER ORAL MUCOSA RECEIVING THERAPY WITH MONOCLONAL ANTIBODIES - CETUXIMAB

Problems in oncology ◽

10.37469/0507-3758-2019-65-5-672-677 ◽

2019 ◽

Vol 65 (5) ◽

pp. 672-677

Author(s):

Yelena Frantsiyants ◽

Irina Neskubina ◽

Lyubov Vladimirova ◽

Aza Lyanova ◽

Yuliya Pogorelova ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Growth Factors ◽

Cell Carcinoma ◽

Oral Mucosa ◽

Squamous Cell ◽

Blood Levels ◽

Partial Regression ◽

Initial Values ◽

Carcinoma Of The Tongue ◽

Insulin Like Growth Factors

The purpose of the study was to measure blood levels of insulin-like growth factors in patients with squamous cell carcinoma of the tongue and lower oral mucosa depending of the efficacy of cetuximab chemotherapy. The study included the data on 50 patients with squamous cell carcinoma of the tongue and lower oral mucosa T3-4N0-1M0. Patients received chemotherapy with cetuximab (n=30, main group) and without it (n=20, controls). Results were compared with the values in 20 non-cancer donors. Both groups of patients were divided into two subgroups: with stable disease and partial regression. Before treatment, blood serum levels of the two insulinlike growth factors IGF-1 and IGF-2 in patients were lower than in donors by 53.5% and 20.3%, respectively. A cycle of the standard chemotherapy in the subgroup with partial regression statistically significantly increased IGF-1 levels by 33% compared to the values before treatment, while IGF-2 was both within donor and initial values and the IGF-1/IGF- 2 ratio was similar to the initial level but 33% lower than in donors. Cetuximab chemotherapy in patients with partial regression normalized IGF-1 levels increasing it compared to initial values - by 87%. IGF-2 levels did not differ statistically significantly from their initial values and were 32.5% lower than in donors. The IGF-1/IGF-2 ratio was 58% higher than before treatment. Two therapy types demonstrated than cetuximab chemotherapy normalized IGF-1 in patients with partial tumor regression shown by the IGF-1 increase up to normal blood levels in effective treatment.

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Study of EGFR expression in tumor tissue in patients with locally advanced oral cavity cancer receiving cetuximab therapy

Medical Council ◽

10.21518/2079-701x-2020-9-182-189 ◽

2020 ◽

pp. 182-189

Author(s):

A. A. Lyanova ◽

L. Yu. Vladimirova ◽

E. P. Ulianova ◽

A. E. Storozhakova ◽

N. A. Abramova ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Targeted Therapy ◽

Cell Carcinoma ◽

Oral Mucosa ◽

Squamous Cell ◽

Locally Advanced ◽

Control Group ◽

Egfr Expression ◽

Tumor Tissues ◽

Carcinoma Of The Tongue

Introduction: Squamous cell carcinoma of the oral cavity is one of the most common head and neck cancers with an aggressive course and high mortality rates. The aim of the study was to determine the EGFR expression levels in tumor tissues in patients with squamous cell carcinoma of the tongue and oral mucosa depending on the efficacy of the therapy. Material and methods: The study included 60 patients with squamous cell carcinoma of the tongue and oral mucosa T3-4N0-1M0. The main group included 30 patients receiving chemotherapy (cisplatin/fluorouracil) in combination with targeted therapy with cetuximab. The control group included 30 patients receiving chemotherapy without cetuximab. Both groups were divided into two subgroups: sensitive and resistant. Results: In treatment-resistant patients of the main group with cetuximab, the average EGFR expression was twice lower than the initial levels (p = 0.0080) and 1.7 times higher than in treatment-resistant patients of the control group (p = 0.0157). In treatment-sensitive patients, the average EGFR expression was 19.8 times lower (p = 0.0020) than initial values and 14.9 times higher (p = 0.0067) than in treatment-sensitive controls. Conclusions: A natural decrease in the EGFR expression in tumor tissues due to the targeted therapy was revealed. However, some patients were resistant to cetuximab, which dictates the need to search for predictors of targeted therapy efficacy in patients with locally advanced squamous cell carcinoma of the tongue and oral mucosa.

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MOLECULAR BASIS OF MODERN TARGETED THERAPY FOR SQUAMOUS CELL CARCINOMA OF THE TONGUE AND ORAL MUCOSA WITH MONOCLONAL ANTIBODIES

Malignant tumours ◽

10.18027/2224-5057-2017-7-4-77-87 ◽

2017 ◽

Vol 7 (4) ◽

pp. 77-87 ◽

Cited By ~ 3

Author(s):

A. A. Lyanova ◽

L. Yu. Vladimirova ◽

E. M. Frantsiyants ◽

D. S. Kutilin ◽

М. A. Engibaryan

Keyword(s):

Squamous Cell Carcinoma ◽

Monoclonal Antibodies ◽

Targeted Therapy ◽

Cell Carcinoma ◽

Oral Mucosa ◽

Squamous Cell ◽

Molecular Basis ◽

Carcinoma Of The Tongue

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From molecular diagnostics to molecular targeted therapy with natural product small molecule inhibitors in oral squamous cell carcinoma

Planta Medica ◽

10.1055/s-2007-986722 ◽

2007 ◽

Vol 73 (09) ◽

Cited By ~ 1

Author(s):

VB Konkimalla ◽

VL Suhas ◽

NR Chandra ◽

E Gebhart ◽

T Efferth

Keyword(s):

Squamous Cell Carcinoma ◽

Targeted Therapy ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Natural Product ◽

Molecular Diagnostics ◽

Small Molecule ◽

Squamous Cell ◽

Molecular Targeted Therapy ◽

Small Molecule Inhibitors

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Spindle Cell Carcinoma of the Tongue: Fallacies in Its Diagnosis

International Journal of Innovative Research in Medical Science ◽

10.23958/ijirms/vol03-i02/14 ◽

2018 ◽

Vol 3 (02) ◽

Author(s):

Amrit Kaur Kaler, Shweta C, Smitha Chandra B.C, Rajeev Naik

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Cavity ◽

Cell Carcinoma ◽

Cell Population ◽

Squamous Cell ◽

Spindle Cell ◽

Spindle Cell Carcinoma ◽

Biphasic Tumor ◽

Carcinoma Of The Tongue ◽

Ihc Markers

Spindle cell carcinoma is a rare aggressive biphasic tumor, composed of neoplastic proliferation of both epithelial (squamous) and spindle cell population. It constitutes about 1% of all oral cavity tumors 2a and is almost rare on the tongue; only few cases have been reported so far. This variant of squamous cell carcinoma, comprises major diagnostic problems due to its varied histomorphology and resemblance to sarcomatous lesion; hence diligent screening and IHC markers are mandatory for its diagnosis.

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Different Aspects of Head and Neck Squamous Cell Carcinoma: Cancer Stem Cells, Their Niche and Targeted Therapy

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666200921163326 ◽

2020 ◽

Vol 15 ◽

Author(s):

Meriç Bilgiç Küçükgüven ◽

Betül Çelebi-Saltik

Keyword(s):

Stem Cells ◽

Squamous Cell Carcinoma ◽

Targeted Therapy ◽

Cancer Stem Cells ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Crucial Role ◽

Tumor Therapy ◽

Neck Squamous Cell Carcinoma

: Head and Neck Squamous Cell Carcinoma (HNSCC) is categorized as the sixth most common cancer worldwide, with an incidence of more than 830,000 cases per year and a mortality rate of 50%. Tobacco use, alcohol consumption, and Human Papillomavirus infection are the prominent risks for HNSCC. Despite significant developments in the treatment of HNSCC, a high rate of recurrences makes the clinical situation worse and results in poor survival rates. Recent perspectives demonstrate that whereas epithelial transformation plays a crucial role in cancer development, tumor surrounding microenvironment takes part in progression of cancer as well. Cancer Stem Cells (CSCs), which harbor unlimited self-renewal capacity, have a crucial role in the growth of HNSCC and this cell population is responsible for tumor recurrence unless eliminated by targeted therapy. CSCs are not only a promising target for tumor therapy, but also a crucial biomarker to determine the patients at high risk for undetermined results and disease development. Just as the bone marrow which is the niche of hematopoietic and mesenchymal stem cells, is important for stem cells maintenance. Similarly, the concept of microenvironment is also important for the maintenance of CSCs. Apart from the cell-cell interactions, there are many parameters in the cancer microenvironment that affect the development of cancer, such as extracellular regulation, vascularization, microbial flora, pH and oxygenation. The purpose of this review is to introduce HNSCC, explain the role of CSCs and their microenvironment and refer to the conventional and novel targeted therapy for HNSCC and CSCs.

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