Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats

BACKGROUND Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . METHODS This experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method. RESULTS Mean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. CONCLUSION Sleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.

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Effect of various inductions of sleep deprivation stress on proinflammatory cytokine levels in gingival crevicular fluids of white male Wistar strain rats (Rattus novergicus)

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v53.i1.p24-29 ◽

2020 ◽

Vol 53 (1) ◽

pp. 24

Author(s):

Pratiwi Nur Widyaningsih ◽

Fitranto Arjadi ◽

Erlina Sih Mahanani

Keyword(s):

Sleep Deprivation ◽

Proinflammatory Cytokines ◽

Paradoxical Sleep ◽

White Male ◽

Control Group ◽

Total Sleep Deprivation ◽

Wistar Strain ◽

Tnf Α ◽

Cytokine Levels ◽

One Way Anova

Background: Stress that is induced by sleep deprivation can modulate the damage of periodontal tissue by elevating the levels of proinflammatory cytokines (i.e. IL-1β and TNF-α). The effects of sleep deprivation can be resolved with sleep recovery. Gingival crevicular fluid (GCF) is fluid in sulcular gingiva which acts as an oral biomarker for evaluating periodontal abnormalities. Purpose: The aim of this study was to determine the effect of various induction methods of sleep deprivation stress on cytokine levels in GCF of white male Wistar strain rats (Rattus novergicus). Methods: The study method was true experimental with a posttest-only control group design. Thirty male Wistar rats were randomly divided into five groups: paradoxical sleep deprivation (PSD), total sleep deprivation (TSD), partial sleep deprivation with sleep recovery for five days (PSD+SR), total sleep deprivation with sleep recovery for five days (TSD+SR) and a healthy control group. Data were analysed via one-way ANOVA to determine differences between groups. Result: The results showed the highest level of IL-1β and TNF-α was found in the PSD group. One-way ANOVA analysis showed significant differences (p<0,05) of IL-1β level between PSD and control groups, PSD and PSD+SR groups and PSD and TSD+SR groups; in contrast, the analysis of TNF-α levels showed significant differences (p<0,05) between PSD group to control group, PSD to PSD+SR group and TSD to TSD+SR group. Conclusions: There is an effect of various induction methods of sleep deprivation stress on proinflammatory cytokines (IL-1β and TNF-α).

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Role of Orexin 1 Receptor Blocker SB-334867 On Changes Of Triglyceride And Cholesterol Metabolisminduced By Paradoxical Sleep Deprivation In Adult Male Rats

Sohag Medical Journal ◽

10.21608/smj.2019.45983 ◽

2019 ◽

Vol 23 (2) ◽

pp. 1-6

Author(s):

Hoda Ahmed ◽

Ahmed Mahmoud ◽

Mohammed Ahmed ◽

Khaled Abdel-Sater

Keyword(s):

Sleep Deprivation ◽

Adult Male ◽

Paradoxical Sleep ◽

Male Rats ◽

Receptor Blocker ◽

Paradoxical Sleep Deprivation

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Effects of paradoxical sleep deprivation on blood parameters associated with cardiovascular risk in intact and ovariectomized rats compared with male rats

Behavioural Brain Research ◽

10.1016/j.bbr.2006.09.012 ◽

2007 ◽

Vol 176 (2) ◽

pp. 187-192 ◽

Cited By ~ 14

Author(s):

I ANTUNES ◽

M ANDERSEN ◽

T ALVARENGA ◽

S TUFIK

Keyword(s):

Cardiovascular Risk ◽

Sleep Deprivation ◽

Paradoxical Sleep ◽

Blood Parameters ◽

Male Rats ◽

Ovariectomized Rats ◽

Paradoxical Sleep Deprivation

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Role of Superoxide Dismutases (SODs) in Hypertensive Rats After Administration of Mango Mistletoe Methanolic Extract

JSMARTech ◽

10.21776/ub.jsmartech.2021.002.02.61 ◽

2021 ◽

Vol 2 (2) ◽

pp. 061-064

Author(s):

Mariyam Suroyya ◽

◽

Nour Athiroh Sjakoer ◽

Nurul Mubarakati ◽

◽

...

Keyword(s):

Methanolic Extract ◽

White Male ◽

Male Rats ◽

Control Group ◽

Hypertensive Rats ◽

Treatment Groups ◽

Group A ◽

The One ◽

Endogenous Antioxidant ◽

Post Hoc

Hypertension is a condition where there is an abnormal rise in blood pressure that may be the primary cause of cardiovascular disease. Hypertension induces the production of free radicals known as reactive oxygen species (ROS) and oxidative stress. The purpose of this study is to further examine the function of Dendropthoe pentandra as an endogenous antioxidant modulator in this case superoxide dismutase (SOD) in hypertensive rats.The testing approach used is experimental. Data were analyzed using the one-way ANOVA test and the Post Hoc test to see variations in SOD levels in different treatments. This research used a hypertensive rat model induced by deoxycorticosterone acetate (DOCA) and salt. The number of animals tested was 25 white male rats divided into 5 groups, each containing 5 rats.The group consisted of a control group, a group of non- Extract methanolic of mango mistletoe hypertensive rats, and three groups of hypertensive rats receiving mango mistletoe methanolic extract (EMBM) at dosages of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight. Based on the results of the study, it is known that the levels of increased lung SOD with extract methanolic of mango mistletoe dosage variations in all treatment groups were not different. The administration of mango parasite methanolic extract at a dose of 50 mg / kgBW was optimum in increasing lung SOD levels in hypertensive rats.

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