One-dimensional proteomic mapping of embryos danio rerio

In the present study, a proteomic technology combining one-dimensional gel electrophoresis (1DE) with subsequent mass spectrometry (MALDI-TOF-PMF) has been successfully applied for revelation of changes in the protein profile of zebrafish (Danio rerio) 52 hpf embryos. Prior to 1DE separation of zebrafish embryonic proteins, the procedure for obtaining embryos homogenate was optimized by ultrasonic treatment. A total of 84 proteins, including 15 vitellogenins, were identified. It was shown that growing of zebrafish embryos in the medium with doxorubicin (DOX) stimulated Caspase-3 induction and promoted the disappearance of cardiac troponins, both these findings being consistent with literature data on doxorubicin-induced cardiotoxicity. The 1DE-based proteomic mapping approach proposed herein enabled not only to identify proteins but also to register those changes in embryos' proteomic profile that were caused by doxorubicin.

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Analysis of proteins copurifying with the cd4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag based protein detection and evaluation of different solubilization methods

Journal of the American Society for Mass Spectrometry ◽

10.1016/s1044-0305(03)00895-x ◽

2004 ◽

Author(s):

O Bernhard

Keyword(s):

Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Protein Detection ◽

Polyacrylamide Gel ◽

Affinity Tag ◽

One Dimensional

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Proteomic Analysis of SARS Associated Coronavirus Using Two-Dimensional Liquid Chromatography Mass Spectrometry and One-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Followed by Mass Spectroemtric Analysis

Journal of Proteome Research ◽

10.1021/pr034111j ◽

2004 ◽

Vol 3 (3) ◽

pp. 549-555 ◽

Cited By ~ 30

Author(s):

Rong Zeng ◽

Hong-Qiang Ruan ◽

Xiao-Sheng Jiang ◽

Hu Zhou ◽

Lv Shi ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Sodium Dodecyl Sulfate ◽

Proteomic Analysis ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Two Dimensional ◽

Liquid Chromatography Mass Spectrometry ◽

One Dimensional

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Mixture Toxic Impacts and the Related Mechanism of Aflatoxin B1 and Deoxynivalenol on Embryonic Zebrafish (Danio Rerio)

10.21203/rs.3.rs-541606/v1 ◽

2021 ◽

Author(s):

Yanhua Wang ◽

Qiang Wang ◽

Chun Ji ◽

Xiaoxuan Guo ◽

Guiling Yang ◽

...

Keyword(s):

Danio Rerio ◽

Aflatoxin B1 ◽

Caspase 3 ◽

Endocrine System ◽

Zebrafish Embryos ◽

Combined Toxicity ◽

Single Exposure ◽

Comprehensive Investigation ◽

Food And Feed ◽

Cellular Apoptosis

Abstract BackgroundAlthough humans and animals are often simultaneously exposed to a variety of mycotoxins via feed and food consumption, in which aflatoxin B1 (AFB1) and deoxynivalenol (DON) is one of the most prevalent combination of mycotoxins. Many toxicological reports have merely focused on the impacts of single mycotoxins. In the present study, the combined toxicity and the related mechanism of AFB1 and DON to zebrafish (Danio rerio) were investigated. ResultsWe showed that DON had lower toxicity to embryonic zebrafish with a 7-day LC50 of 218.3 mg a.i. L-1 in comparison to AFB1 (0.031 mg a.i. L-1). The mixture of AFB1 and DON elicited an additive combined effect on zebrafish embryos. The levels of CAT, caspase-3, and T4 markedly varied in most single and mixture groups. The expressions of four genes (cas3, apaf-1, cc-chem, and cyp19a) associated with oxidative stress, cellular apoptosis, immune system, and endocrine system were markedly varied upon the mixture exposure in comparison to the corresponding single exposure of AFB1 or DON.ConclusionsOur results demonstrate that the impacts of a mixture could not be estimated solely based on toxicities of the single mycotoxins. Taken together, our comprehensive investigation on the mycotoxin mixtures and their potential mechanisms could better reflect the reality of mycotoxin contamination in food and feed.

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