scholarly journals Characteristics of influence of 4-[4-oxo-3 h-quinazoline-3-yl] benzoic acid (pc-66) on a course of adrenaline myocardiodystrophy in rats according to biochemical research

2017 ◽  
Author(s):  
O. V. Dzhyhaliuk ◽  
H. I. Stepaniuk ◽  
N. V. Zaichko ◽  
S. I. Kovalenko ◽  
K. P. Shabelnyk
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Benzoic Acid ◽  
Free Radical Oxidation ◽  
Control Group ◽  
No Production ◽  
Marker Enzymes ◽  
Radical Oxidation ◽  
Group I

According to the literature the leading cause of mortality among the population of Ukraine are cardiovascular diseases, what incidence tripled over the past 25 years and today stands at801.6 in100 000. It necessitates a search for new chemical compounds, suitable for creating manufacturing with a better safety profile and a high degree of efficiency.The aim of the study was to describe the effect of 4-[4-oxo-4N-quinazoline-3- il] benzoic acid (PC-66), compared with kordaron on the processes of free radical oxidation, endothelial nitric oxide production and activity of marker enzymes cytolysis cardiomyocytes adrenaline myocardiodystrophy (AMD) in rats.The study was conducted on 28 nonlinear rats of both sexes (weight – 165–220 grams), divided into four groups: group I (n = 7) – intact rats; group II (n = 7) – rats with untreated AMD (control); group III (n = 7) – rats with AMD treated with entity PC-66 (10 mg/kg intraperitoneally (i/p)); Group IV (n = 7) – rats with AMD treated with kordaron (10 mg/kg, i/p).Research shown that the development of a pilot AMD in rats was characterized by increased free radical oxidation processes, depletion of antioxidant defense system (with decreased content of G-SH, activity of superoxide dismutase and glutathione peroxidase), oppression of endothelial NO production. The most expressive metabolic disturbances were registered in the acute period of AMD (as of 2nd day). The use of 4-[4-oxo-4N-quinazoline-3-il] benzoic acid (PC-66, 10 mg/kg, i/p) provided an expressive cardioprotective effect which was compared with kordaron (10 mg/kg, i/p) at various stages of AMD.The mechanisms of cardioprotective action 4-[4-oxo-4N-quinazoline-3-il] benzoic acid can be realized due to the stabilization of cellular and subcellular membranes (as evidenced by the dynamics of marker enzymes cytolysis cardiomyocytes in serum and reduction of TBA-reactive substances in the myocardium), increasing the efficiency of the antioxidant system and correction of cellular redox buffer (as evidenced by the increasing pool of reduced glutathione, glutathione peroxidase and superoxide dismutase activity increased) and anti-ischemic activity associated with increased activity of endothelial NO synthase in cardiac muscle.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

scholarly journals Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes

2021 ◽  
Vol 11 (3) ◽  
pp. 12-17
Author(s):  
A. V. Elikov
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Former Athletes ◽  
Oxidative Balance

Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.

Analysis of the content of zinc ions in the ejaculate of men with inflammatory diseases of the genitourinary system with indicators of sperm motility

2021 ◽  
pp. 28-32
Author(s):  
O.A. Gizinger
Keyword(s):  
Superoxide Dismutase ◽  
Plasma Membrane ◽  
Free Radical ◽  
Seminal Plasma ◽  
Inflammatory Diseases ◽  
Free Radical Oxidation ◽  
Zinc Ions ◽  
Radical Oxidation ◽  
Genitourinary System ◽  
Sperm Dna

The results of previous studies have shown that the content of zinc ions in the semen significantly correlated with the number of sperm and influenced the volume of ejaculate. After ejaculation, the zinc contained in the seminal plasma binds to the plasma membrane and stabilizes the sperm DNA. Due to its cyto- and immunoprotective properties: induction of zinc-dependent superoxide dismutase, protection of DNA and transcriptional proteins from free radical oxidation, inhibition of proteinases, zinc is an indispensable trace element in the processes of DNA synthesis and repair, embryogenesis and reproduction.

scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

scholarly journals The aspects of free radical oxidation in the lungs of rats with pleurisy under long-term lighting

2017 ◽  
Author(s):  
O. M. Larycheva ◽  
L. D. Chebotar ◽  
T. Ya. Yaroshenko
Keyword(s):  
Free Radical ◽  
Electron Transport ◽  
Pineal Gland ◽  
Light Exposure ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Ros Generation ◽  
Radical Oxidation ◽  
Carrageenan Pleurisy

Introduction.Pathological changes in human body due to light exposure at night are triggered by circadian disruption, inhibition of melatonin secretion by the pineal gland at night that leads to its reduction in blood. Clinical and experimental data indicate high importance of free radical oxidation inthe pathogenesis of pulmonary diseases. The aim of the study – to explore the influence of a melatonin deficiencyin addition to pleurisy on free radical oxidation in the tissues of the lungs of rats exposed to light for 30 days.Research Methods. Experiments were carried out on male albino Wistar rats. The animals were randomized into four groups with 7 animals in each: an intact group, 30 day pineal gland hypofunction, carrageenan pleurisy, 30 day pineal gland hypofunction with carrageenan pleurisy. Pineal gland hypofunction was modeled by 1500 lx fluorescent lamps around the clock. The experimental pleurisy was induced in anesthetized animals by intrapleural injection of 0.1 ml of carrageenan. Superoxide (•О2-) products were determined by the reaction with nitro blue tetrazolium under the influence of NADH, NADP and pirogenal. The concentration of diene conjugates was determined by I.D. Stalna technique, TBA-active products – by I.D. Stalna, T.H. Harishvili method, trienes, ketodienes, - by a modified method based on the molar extinction coefficient.Results and Discussion.Assessingthe intensity of the reactive oxygen species (ROS) generation, a significantincrease of the •О2-level in all sources of its generation in the tissues of the lungs has been observed both in the control group with pleurisy and in the rats with 30-day pineal gland hypofunctionandpleurisy, compared to the intact animals.The chronic pineal gland hypofunction in the animals with experimental pleurisy lead to •О2- generationby the microsomal electron transport chain (ETC) by 54.6 % (р<0.05) when compared with carrageenan group of rats. No significant changes in concentration of the lipid peroxidation products has been observed in the animals with pleurisy during long-term light exposure.Conclusions. Chronic hypofunction of the pineal gland with the carrageenan-induced pleurisy causes activation of the free radical processes in the rats’ lung tissues and occur as hyperproduction of the superoxide anion radical by the microsomal chain of oxidation. This fact denotes probable abnormalities in the function of the microsomal electron transport chains especially under the condition change of properties of dehydrogenases. 

scholarly journals Free-Radical Oxidation (Peroxidation) Products in Plasma in Normal and Abnormal Pregnancy

1981 ◽  
Vol 18 (3) ◽  
pp. 158-162 ◽  
Author(s):  
D Wickens ◽  
M H Wilkins ◽  
J Lunec ◽  
G Ball ◽  
T L Dormandy
Keyword(s):  
Blood Pressure ◽  
Free Radical ◽  
Radical Reaction ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Control Group ◽  
Free Radical Reaction ◽  
Reaction Products ◽  
Third Trimester ◽  
Radical Oxidation

Using recently developed methods for measuring free-radical oxidation products in biological material, plasma extracts were studied in 24 women in the first two trimesters of pregnancy, in 124 women in the third trimester of pregnancy, in 20 women with pre-eclamptic toxaemia (PET), and in a control group. There was a significant progressive rise of two groups of free-radical oxidation products throughout pregnancy and a significantly greater rise in PET. In women whose diastolic blood pressure rose to above 70 mmHg there was a highly significant relation between two groups of free-radical reaction products and blood pressure.

scholarly journals Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Igor Y. Iskusnykh ◽  
Tatyana N. Popova ◽  
Aleksander A. Agarkov ◽  
Miguel Â. A. Pinheiro de Carvalho ◽  
Stanislav G. Rjevskiy
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Toxic Hepatitis ◽  
Free Radical Oxidation ◽  
Inflammatory Cells ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.

scholarly journals S-ADEMETHIONINE EFFECT ON THE ACTIVITY OF FREE RADICAL OXIDATION PROCESSES AND ARGININE/CITRULLINE CYCLE WITH DOXORUBICIN-INDUCED LIVER INJURY

2020 ◽  
Vol 20 (2) ◽  
pp. 156-161
Author(s):  
G.S. Maslova ◽  
R.I. Skrypnyk ◽  
І.М. Skrypnyk
Keyword(s):  
Free Radical ◽  
Sodium Chloride ◽  
Liver Injury ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Arginase Activity ◽  
Radical Oxidation ◽  
Group I ◽  
Doxorubicin Administration

Seeking for effective methods for doxorubicin-induced toxicity prevention is of great clinical importance. The aim of the study is to investigate the effect of S-ademethionine on the activity of free radical oxidation and antioxidant protection and arginine / citrulline cycle, and the relationship between the indicators during the modeled doxorubicin-induced liver injury in rats. Materials and methods. The series of study were performed on 30 white non-linear adult rats, 15 (50%) males, 15 (50%) females, weighing 160-220 g. The experimental animals were divided into 3 groups: I group (n=10) included rats (5 males and 5 females), which during 3 days were intraperitoneally administered with doxorubicin of 5 mg/kg/day to achieve cumulative dose of 15 mg/kg and 0.9% sodium chloride solution 1 ml; ІІ group (n=10) included rats (5 males and 5 females), which for 3 days were administered with doxorubicin in dosage similar to that in group I and S-ademethionine intraperitoneally in a dose of 100 mg/kg/day with a total dose of 300 mg/kg; ІІІ group (n=10) included rats (5 males and 5 females), which were injected intraperitoneally for 3 days with 0.9% sodium chloride 1 ml solution by 2 injections. Thiobarbituric acid reactants, arginine, catalase, arginase and ornithine decarboxylase activity were studied in the liver homogenate. Results. The rats of group I in the course of doxorubicin administration demonstrated the growth of the thiobarbituric acid reactants in 2.57 times (p=0.002) in the liver homogenate that was accompanied by 1.7-fold decreased arginase activity compared to the control group III. An inverse correlation was found between the content of thiobarbituric acid reactants and arginase activity in the liver homogenate of group I rats (r=-0.71; p=0.02). Coadministration of S-ademethionine and doxorubicin administration led to 1.4-fold increase in catalase activity in the liver homogenate of rats compared to the control (p=0.009) that reduced the severity of oxidative stress and prevented changes in the arginine/citrulline cycle. Conclusions: S-ademethionine combined with doxorubicin, reduces the activity of free radical oxidation by potentiating the production of antioxidant protective enzymes that prevent impairments in the arginine / citrulline cycle.

scholarly journals Effect of Leu-Ile-Lys tripeptide on the activity of free radical oxidation and expression of сyclooxygenase-1 and -2 in gastric mucosa in experimental indomethacin-induced ulcer in rats

2019 ◽  
Vol 100 (3) ◽  
pp. 439-444
Author(s):  
S E Lorents ◽  
E N Mazko ◽  
O G Makarova ◽  
A Yu Zharikov ◽  
Yu V Korenovskiy ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastric Mucosa ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Intragastric Administration ◽  
Radical Oxidation ◽  
Cyclooxygenase 1 ◽  
Total Antioxidant ◽  
Male Wistar Rats

Aim. To determine the effect of Leu-Ile-Lys tripeptide on indicators of oxidative stress and expression of cyclooxygenase-1 and -2 in the gastric mucosa on the background of experimental indomethacin-induced ulcer. Methods. Experiments were performed on 35 male Wistar rats with a body mass of 200-250 g aged 2-3 months that were divided into 3 groups: the control group (intact rats, 8 animals), control group (simulation of indomethacin-induced gastropathy, 12 animals) experimental group (simulation of indomethacin-induced gastropathy + administration of Leu-Ile-Lys tripeptide, 15 animals). The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity at least 98%) was administered intragastrically daily for 7 days before the simulation of indomethacin-induced damage of the gastric mucosa in a dose of 11.5 mg/kg. The model of indomethacin-induced damage to the rodent gastric mucosa was reproduced by a single intragastric administration of indomethacin in a dose of 60 mg/kg in 1 ml of saline. In the stomach homogenate the activity of free radical oxidation was determined by conventional methods. For quantitative determination of cyclooxygenase-1, -2 in gastric homogenate the method of enzyme immunoassay was used. The concentration was determined spectrometrically by the color intensity of the samples. Results. With a prophylactic administration of tripeptide Leu-Ile-Lys, concentration of tiobarbiturate-reactive products was normalized, total antioxidant activity increased and the activity of antioxidant enzymes decreased compared to the control group. The concentration of cyclooxygenase-2 in gastric homogenate from the experimental animals was 2.3 times lower than that of the control rats. Conclusion. Use of Leu-Ile-Lys tripeptide in experimental indomethacin-induced gastric ulcer showed a significant decrease of oxidative damage and inflammation in the gastric mucosa.

scholarly journals Intensity of free radical processes in cartilage of rats with collagen-induced arthritis

2016 ◽  
Vol 21 (2) ◽  
pp. 27-30
Author(s):  
E. Tihova ◽  
O. Bernyk ◽  
O. Savchuk ◽  
K. Dvorshchenko ◽  
L. Ostapchenko
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Experimental Model ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Free Radical Oxidation ◽  
Collagen Induced Arthritis ◽  
Radical Oxidation ◽  
Radical Processes

Products of free radical oxidation and antioxidant enzymes in rats with collagen-induced arthritis were studied. It was established, that under experimental model of arthritis in rat cartilage the content of superoxide anion radical, peroxide and TBA-active compounds and the activity of antiradical enzymes superoxide dismutase and catalase increased.

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