scholarly journals Functional activity of NADH-dependent reductase system in liver and guerin’s carcinoma microsomal fraction in rats exposed to preliminary irradiation

2013 ◽  
Vol 59 (6) ◽  
pp. 662-672
Author(s):  
M.M. Marchenko ◽  
O.V. Ketsa
Keyword(s):  
Tumor Growth ◽  
Cytochrome B ◽  
Functional Activity ◽  
Microsomal Fraction ◽  
Reductase Activity ◽  
Enzymatic Activities ◽  
Tumor Bearing ◽  
Guerin’S Carcinoma ◽  
Nadh Cytochrome ◽  
Preliminary Irradiation

The activity of liver and Guerin’s carcinoma microsomal NADH-dependent reductase system has been investigated in tumor-bearing rats exposed to preliminary irradiation. Preliminary irradiation of rats (before transplantation of Guerin’s carcinoma) resulted in the decrease of NADH-cytochrome b reductase activity and contents of cytochrome b in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of oncogenesis compared with the non-irradiated tumor-bearing rats. The effect of irradiation preceding transplantation of the tumor to rats results in the increase of enzymatic activities of liver microsomal NADH-cytochrome b reductase in the latent and logarithmic phases of tumor growth as compared with non-irradiated tumor-bearing rats. At the same time the contents of cytochrome b decreases. During longer periods after irradiation the investigated parameters approached to those in the group of non-irradiated tumor-bearing animals.

scholarly journals Effect of laser irradiation on xanthine oxidase activity and superoxide radical generation in rat liver cytosol fraction

2020 ◽  
Vol 22 (2) ◽  
pp. 54-57
Author(s):  
O. V. Ketsa ◽  
◽  
N. B Kutsak ◽  
M. M. Marchenko ◽  
◽  
...  
Keyword(s):  
Tumor Growth ◽  
Enzymatic Activity ◽  
Xanthine Oxidase ◽  
Laser Irradiation ◽  
Superoxide Radical ◽  
Cytosolic Fraction ◽  
Sh Groups ◽  
Tumor Bearing ◽  
Guerin’S Carcinoma ◽  
Radical Generation

The effect of tumor growth in the body and laser irradiation on the enzymatic activity of xanthine oxidase, in particular its D- and O-forms, and also the rate of generation of the superoxide radical (O2–) and the level of protein sulfhydryl groups in the liver rat cytosolic fraction has been investigated. It has been found that in the cytosolic fraction of rats with transplanted Guerin’s carcinoma decreases the enzymatic activity of the D-form of xanthine oxidase with a simultaneous increase in its O-form during the period of intensive (14 days, which corresponds to the logarithmic phase of on cogenesis) and the period of final tumor growth (21 days, which corresponds to the stationary phase of oncogenesis). The increase in the enzymatic activity of the O-form of xanthine oxidase was accompanied by an increase the rate of superoxide radical generation and a decrease in the level of protein SH-groups in the liver cytosolic fraction of tumor-bearing rats. Daily directed action of laser irradiation on the area of growth of Guerin’s carcinoma leads to less destructive changes in the liver. Thus, there is an increase in the enzymatic activity of the D-form of xanthine oxidase, a decrease the rate of superoxide radical formation and an increase the content of protein SH-groups in the cytosolic fraction of the liver of experimental animals compared with non-irradiated tumor-bearing rats.

scholarly journals Aminotransferase activity in rat blood serum under malignant guerin's carcinoma growth upon treatment with antioxidant drug "GRIN"

2014 ◽  
Vol 68 (3) ◽  
pp. 64-67
Author(s):  
Ya. Raetska ◽  
O. Morgaienko ◽  
T. Ischuk ◽  
L. Ostapchenko
Keyword(s):  
Tumor Growth ◽  
Blood Serum ◽  
Inhibitory Effect ◽  
Enzymatic Activities ◽  
Aminotransferase Activity ◽  
Malignant Growth ◽  
Rat Blood ◽  
Guerin’S Carcinoma

The malignant growth of Guerin's carcinoma was shown to induce increase of aminotransferases' enzymatic activities. It was established that introduction of the antioxidant substance "GRIN" led to normalization of aminotransferases' activities in blood serum and also had essential inhibitory effect on the tumor growth.

ROLE OF MITOCHONDRIAL NO-SYNTHASE IN THE IMPLEMENTATION OF ANTITUMOR EFFECTS OF POLYUNSATURATED FATTY ACIDS IN THE MODEL OF GUERIN’S CARCINOMA UNDER IN VIVO CONDITIONS

2018 ◽  
Vol 64 (1) ◽  
pp. 138-143
Author(s):  
Mikhail Marchenko ◽  
Igor Shmarakov ◽  
O. Ketsa
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Tumor Growth ◽  
Mitochondrial Fraction ◽  
No Synthase ◽  
Antitumor Effects ◽  
Pufa Supplementation ◽  
Tumor Mass ◽  
Tumor Bearing ◽  
Guerin’S Carcinoma

NO-synthase activity and NO level in the mitochondrial fraction of rats Guerin’s carcinoma under conditions of different ю-3 and ю-6 polyunsaturated fatty acids (PUFAs) supplementation has been investigated. Guerin’s carcinoma was used as a cancer model. 0.5 ml of 30% carcinoma cell suspension in normal saline was implanted subcutaneously into thigh of a hind leg. The intensity of tumor growth was evaluated based on measurements of subcutaneous tumor size and coefficient of tumor mass. It was showed that ω-3 PUFAs supplementation before and after transplantation of Guerin’s carcinoma resulted in the decreases of tumor growth and coefficient of tumor mass in an organism. Additional ю-6 PUFA supplementation can stimulate tumor progression and increase tumor mass in body in the logarithmic phases of carcinogenesis as compared to the tumor-bearing rats. The effect depends on duration of ю-3 and ю-6 PUFAs administration and on the level of preliminary provision of an organism with these PUFAs. We found increased NO-synthase activity and NO content in mitochondrial fraction of Guerin’s carcinoma in animals of the group that was administered ю-3 PUFAs both before and post-implantation of the carcinoma during logarithmic phase of carcinogenesis. At the same time, the ю-6 PUFA administration does not contribute to significant changes in the NO-synthase system components in the mitochondrial fraction of Guerin’s carcinoma in comparison with the tumor-bearing rats.

scholarly journals Adrenal microsomal C19-steroid 5α-reductase activity in the Snell transplantable rat adrenocortical tumour 494 and the effect of oestradiol, testosterone propionate and adrenocorticotrophin in intact and gonadectomized rats

1973 ◽  
Vol 132 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Paul V. Maynard ◽  
Euan H. D. Cameron
Keyword(s):  
Enzyme Activity ◽  
Steroid Hormones ◽  
Microsomal Fraction ◽  
Testosterone Propionate ◽  
Reductase Activity ◽  
Intact Control ◽  
Adrenal Tissue ◽  
Males And Females ◽  
Adrenocortical Tumour ◽  
Hormonal Treatments

The C19-steroid 5α-reductase activity in the microsomal fraction of rat adrenal tissue under various hormonal treatments was examined. In intact control rats the activity is similar in both males and females, and after gonadectomy it is markedly increased. Treatment with oestradiol (150μg/day per animal for 7 days) or testosterone propionate (2mg/day per animal for 7 days) lowered the activity of 5α-reductase in castrated animals to approximately the values for intact animals in both sexes, and in intact animals the activity was also decreased by these treatments. The enzyme activity was also decreased by adrenocorticotrophin treatment but to a lesser extent than by the steroid hormones. The activity of the 5α-reductase enzyme in the Snell adrenocortical tumour 494 is very low when incubated as a whole homogenate, but the activity in microsomal material of the tumour was measured and unexpectedly found to be similar to that in intact controls.

Anti-angiogenic inhibition of tumor growth by systemic delivery of PEI-g-PEG-RGD/pCMV-sFlt-1 complexes in tumor-bearing mice

2006 ◽  
Vol 114 (3) ◽  
pp. 381-388 ◽  
Author(s):  
Won Jong Kim ◽  
James W. Yockman ◽  
Ji Hoon Jeong ◽  
Lane V. Christensen ◽  
Minhyung Lee ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Systemic Delivery ◽  
Tumor Bearing ◽  
Inhibition Of Tumor Growth

scholarly journals N-myristoylation determines dual targeting of mammalian NADH-cytochrome b(5) reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning

2005 ◽  
Vol 168 (5) ◽  
pp. 735-745 ◽  
Author(s):  
Sara Colombo ◽  
Renato Longhi ◽  
Stefano Alcaro ◽  
Francesco Ortuso ◽  
Teresa Sprocati ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Signal Recognition Particle ◽  
Phospholipid Bilayer ◽  
Mitochondrial Outer Membrane ◽  
Signal Recognition ◽  
Outer Membranes ◽  
Dual Targeting ◽  
Nascent Chain ◽  
Hydrophobic Amino Acids ◽  
Nadh Cytochrome

Mammalian NADH-cytochrome b(5) reductase (b5R) is an N-myristoylated protein that is dually targeted to ER and mitochondrial outer membranes. The N-linked myristate is not required for anchorage to membranes because a stretch of hydrophobic amino acids close to the NH2 terminus guarantees a tight interaction of the protein with the phospholipid bilayer. Instead, the fatty acid is required for targeting of b5R to mitochondria because a nonmyristoylated mutant is exclusively localized to the ER. Here, we have investigated the mechanism by which N-linked myristate affects b5R targeting. We find that myristoylation interferes with interaction of the nascent chain with signal recognition particle, so that a portion of the nascent chains escapes from cotranslational integration into the ER and can be post-translationally targeted to the mitochondrial outer membrane. Thus, competition between two cotranslational events, binding of signal recognition particle and modification by N-myristoylation, determines the site of translation and the localization of b5R.

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