Prevalence of Helicobacter pylori in patients with peptic ulcer disease

Background: Prevalence of Helicobacter pylori (H. pylori) emerges throughout the world and instigates peptic ulcer disease (PUD). The study was conducted with the aim to determine the prevalence of H. pylori in patients with PUD undergoing upper gastrointestinal endoscopy.Methods: This prospective study was conducted on 150 cases of PUD from August 2009 to February 2011. Endoscopy was done in all cases. Biopsy was done and sent for histopathological examination and rapid urease test for confirmation of presence of H. pylori.Results: Out of 150 patients with mean age of 45.76 years, 109 patients were diagnosed to have been infected with Helicobacter pylori (72.66%). Out of 89 patients with gastric ulcer, 61 patients were infected with Helicobacter pylori (68.53%). Forty two out of 51 patients (82.35%) with duodenal ulcers and 06 of 10 patients (60%) with carcinoma of stomach were positive for H. pylori. The remaining patients were found to be negative for the H. pylori infection.Conclusions: The findings of the study conclude that H. pylori was consistently associated with PUD.

Download Full-text

The prevalence of Helicobacter pylori in perforated peptic ulcer disease

International Surgery Journal ◽

10.18203/2349-2902.isj20181405 ◽

2018 ◽

Vol 5 (5) ◽

pp. 1720

Author(s):

Babar Rehmani ◽

Priyank Pathak

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Rapid Urease Test ◽

Peptic Ulcer Perforation ◽

Mucosal Biopsy ◽

Ulcer Disease ◽

Ulcer Perforation ◽

Urease Test ◽

H Pylori

Background: Although the role of Helicobacter pylori infection in noncomplicated peptic ulcer disease has been definitively established, the precise relationship between the organism and ulcer complications is doubtful. Recurrent ulcer disease after peptic ulcer perforation mainly occurs in patients with H. pylori infection, which suggests that the microorganism plays an important role in this complication.Methods: This observational study was conducted in the Department of General Surgery, Himalayan Institute of Medical Sciences (HIMS), Swami Ram Nagar, Dehradun, over a period of January 2013 to December 2013 and included 75 subjects who underwent exploratory laparotomy for peptic ulcer perforation. The tests used for the diagnosis of H. pylori are mucosal biopsy at the time of surgery, Rapid urease test (RUT), Stool antigen test.Results: In this study, H. pylori infection was found to be present in 61% of these patients as detected by biopsy. There were 66 males and 9 females. Infection with H. pylori is almost universal in patients aged more than 70 years.Conclusions: In the Indian context patients presenting with perforation should be tested for infection with H. pylori utilizing a gastric antral mucosal biopsy taken at the time of operation for histological analysis and eradication therapy should be advised to all those who are found positive.

Download Full-text

A STUDY ON PREVALENCE OF HELICOBACTER PYLORI IN BLEEDING PEPTIC ULCER USING RAPID UREASE TEST

10.36106/ijsr/4914430 ◽

2021 ◽

pp. 82-83

Author(s):

Lokesh M N ◽

Anil Kumar K N ◽

Madan M

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Bleeding Peptic Ulcer ◽

Ulcer Bleeding ◽

Rapid Urease Test ◽

Ulcer Disease ◽

Urease Test ◽

H Pylori

BACKGROUND: Helicobacter pylori (H. pylori) is the leading cause of peptic ulcer disease in India. Timely diagnosis and proper treatment is required for the eradication of H. pylori infection and prevention of its associated complications. AIMS AND OBJECTIVES: This study aims to evaluate the prevalence of H. pylori infection in patients presenting with bleeding peptic ulcer. MATERIAL AND METHODS: This is prospective study conducted from January 2019 till January 2021 in patients with bleeding peptic ulcer admitted at BGS Global Institute of Medical Sciences. All the patients under went endoscopy and biopsy was taken from Antral region for Rapid Urease Test. RESULT: 164 patients were included in the study with duodenal ulcer bleeding in 129 (78.6%) patients, gastric ulcer in 30 (18.3%) patients and both gastric and duodenal ulcer in 5 (3.1%) patients. Positivity for RUT was seen in 147 patients (89.6%). CONCLUSION: Early intervention in the patients with peptic ulcer disease in the form of endoscopic biopsy will help in prevention of recurrent bleeding episodes and other associated complications.

Download Full-text

A comparative study between benign and malignant conditions of upper gastrointestinal tract of helicobacter pylori using upper gastrointestinal scopy in a tertiary care hospital, Kanchipuram, Tamil Nadu, India

International Surgery Journal ◽

10.18203/2349-2902.isj20170866 ◽

2017 ◽

Vol 4 (3) ◽

pp. 1082

Author(s):

Dhinesh Babu K. ◽

M. Bhaskar

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

The Other ◽

Rapid Urease Test ◽

Gi Tract ◽

Duodenal Ulcers ◽

Ulcer Disease ◽

Upper Gi ◽

Urease Test ◽

Upper Gastrointestinal

Background: Acid peptic disease comprises of a wide spectrum of diseases, which cause considerable morbidity. The objective of this study was to study the prevalence of Helicobacter pylori in patients with dyspepsia and symptomatic patients undergoing upper gastrointestinal endoscopy (UGIE) in Karpaga Vinayaga Medical College and Hospital, Kanchipuram, Tamilnadu, India and to study the association of Helicobacter pylori with acid peptic diseases and malignant conditions of upper Gastro intestinal tract.Methods: 389 cases of dyspepsia, studied clinically, were subjected to UGIE, during which 4 biopsies, two each from the antrum and the pathological areas were taken. One of the antral area and the other of the pathological finding were immediately subjected to Rapid urease test. Positive test for Helicobacter pylori was indicated by change in colour of the medium from yellow to pink or red. The other two biopsy specimens were sent for routine histopathology and special staining with Giemsa stain. The case was taken as Helicobacter pylori positive when the rapid urease test and/or histopathological examination was positive.Results: Out of 468 patients, with mean age of 41.8 years, 171 patients were diagnosed to have been infected with Helicobacter pylori (44.21%). Out of 49 patients with gastric and duodenal ulcers, 37 patients were infected with Helicobacter pylori (75.51%). In which 22 out of 25 patients (88%) with duodenal ulcers and 10 out of 14 patients (71.4%) with gastric ulcers were positive for H. pylori while only 8 out of 10 patients (80%) with gastric cancer were positive for H. pylori.Conclusions: In this study, we found that Helicobacter pylori were consistently associated with peptic ulcer disease and malignant conditions of upper GI tract, which is in broad agreement with the studies done earlier. Thus, we conclude that, Helicobacter pylori infection may have a major role in the etiopathogenesis of peptic ulcer disease and malignant conditions of upper GI tract appear to be no significant association between Helicobacter pylori infection and unexplained dyspepsia.

Download Full-text

Omeprazole, Furazolidone, and Tetracycline: an eradication treatment for resistant H. pylori in Brazilian patients with peptic ulcer disease

Revista do Hospital das Clínicas ◽

10.1590/s0041-87812002000500003 ◽

2002 ◽

Vol 57 (5) ◽

pp. 205-208 ◽

Cited By ~ 12

Author(s):

Fernando Marcuz Silva ◽

Jaime Natan Eisig ◽

Ethel Zimberg Chehter ◽

Júlio Jovino da Silva ◽

Antonio Atílio Laudanna

Keyword(s):

Peptic Ulcer ◽

Adverse Effects ◽

Peptic Ulcer Disease ◽

Breath Test ◽

Low Cost ◽

Rapid Urease Test ◽

Ulcer Disease ◽

Urease Test ◽

Previously Treated ◽

H Pylori

OBJECTIVES: To determine the efficacy of a simple, short-term and low-cost eradication treatment for Helicobacter pylori (H. pylori) using omeprazole, tetracycline, and furazolidone in a Brazilian peptic ulcer population, divided into 2 subgroups: untreated and previously treated for the infection. PATIENTS AND METHODS: Patients with peptic ulcer disease diagnosed by endoscopic examination and infected by H. pylori diagnosed by the rapid urease test (RUT) and histological examination, untreated and previously unsuccessfully treated by macrolides and nitroimidazole, were medicated with omeprazole 20 mg daily dose and tetracycline 500 mg and furazolidone 200 mg given 3 times a day for 7 days. Another endoscopy or a breath test was performed 12 weeks after the end of treatment. Patients were considered cured of the infection if a RUT and histologic examination proved negative or a breath test was negative for the bacterium. RESULTS: Sixty-four patients were included in the study. The women were the predominant sex (58%); the mean age was 46 years. Thirty-three percent of the patients were tobacco users, and duodenal ulcer was identified in 80% of patients. For the 59 patients that underwent follow-up examinations, eradication was verified in 44 (75%). The eradication rate for the intention-to-treat group was 69%. The incidence of severe adverse effects was 15%. CONCLUSION: The treatment provides good efficacy for H. pylori eradication in patients who were previously treated without success, but it causes severe adverse effects that prevented adequate use of the medications in 15% of the patients.

Download Full-text

Helicobacter pylori CagA seropositivity in adult Bangladeshi patients with peptic ulcer and erosion

IMC Journal of Medical Science ◽

10.3329/imcjms.v14i1.47453 ◽

2020 ◽

Vol 14 (1) ◽

pp. 36-40

Author(s):

Fahmida Rahman ◽

Khandaker Shadia ◽

Salma Khatun ◽

Mafruha Mahmud ◽

Indrajit Kumar Dutta ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Enzyme Linked Immunosorbent Assay ◽

Rapid Urease Test ◽

Igg Antibody ◽

Ulcer Disease ◽

Urease Test ◽

Urease Production ◽

Stool Antigen ◽

H Pylori

Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. Methods: Any case that had peptic ulcer/erosion, plus positive for rapid urease test (RUT) or H. pylori stool antigen (HpSAg) or serum anti-H. pylori IgG/IgA were included in the study and named as H. pylori positive case. H. pylori positive cases were tested for CagA IgG antibody. Anti-H. pylori IgG, IgA and CagA IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and stool antigen by rapid immunochromatographic test (ICT). Urease production in biopsy sample was detected by RUT. Results: Total 86 H. pylori positive patients were included in the study. Out of 86 patients, CagA IgG was positive in 34 (39.5%; 95% CI: 0.30,0.50) cases. CagA seropositivity rate in ulcer and erosion cases were 58.8% (95% CI: 0.36,0.78) and 34.8% (95% CI: 0.25,0.47) respectively. H. pylori stool antigen and IgA antibodies were positive in all (100%) CagA antibody positive ulcer cases while the rates were significantly less among the CagA antibody negative cases (42.8% and 28.6%; p<0.05). However, in CagA antibody positive erosion cases, the rates were not significantly different from CagA antibody negative cases. Conclusion: The study has demonstrated that the CagA positive strain is less prevalent in erosion than ulcer cases. Ibrahim Med. Coll. J. 2020; 14(1): 36-40

Download Full-text

Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

International Journal of Microbiology ◽

10.1155/2021/5540560 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Rania M. Kishk ◽

Nashaat M. Soliman ◽

Maha M. Anani ◽

Nader Nemr ◽

Ayman Salem ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Virulence Genes ◽

Histopathological Examination ◽

Virulence Gene ◽

National Study ◽

Rapid Urease Test ◽

Urease Test ◽

Cytotoxin Associated Gene A ◽

H Pylori

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

Download Full-text

Prevalence of helicobacter pylori infection in patients with peptic ulcer disease at Kathmandu Model Hospital

Journal of Chitwan Medical College ◽

10.3126/jcmc.v8i4.23761 ◽

2018 ◽

Vol 8 (4) ◽

pp. 3-7

Author(s):

Rajesh Dhoj Joshi ◽

Sachin Khadka ◽

Deepak Man Joshi ◽

Arun Kadel ◽

Ganesh Dangal ◽

...

Keyword(s):

Duodenal Ulcer ◽

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Rapid Urease Test ◽

Gi Endoscopy ◽

Ulcer Disease ◽

Upper Gi ◽

Urease Test ◽

Upper Gi Endoscopy ◽

H Pylori

Introduction: Endoscopic rapid urease test is a simple and most widely used test to detect the presence of urease in the gastric mucosa. Many studies have reported prevalence of H. pylori infection in relation to age, gender and site of ulcer. Therefore, this study was designed to determine the prevalence and significance of H. pylori in peptic ulcer disease. Methods: A retrospective review was carried out for patients with peptic ulcer disease who had undergone upper GI endoscopy in Department of Internal Medicine at Kathmandu Model Hospital. The records from January 2013 to December 2017 were analyzed. Any patient with previously diagnosed peptic ulcer, history of active bleeding, cancer and incomplete records were excluded. Peptic ulcer associated with H. pylori was diagnosed on the basis of endoscopic rapid urease test. Results: Among the 418 diagnosed case of peptic ulcer disease by upper GI endoscopy from Jan 2013 to Dec 2017, 213 tested positive for H. pylori by rapid urease test. Among the positive cases, over a half were males patients. Majority (23.9%) of the patients were in the age group of 35-44 years. Prevalence of H. pylori in duodenal ulcer was 51.6% followed by combined gastro-duodenal ulcer (26.8%) and gastric ulcer (21.6%). H. pylori was significantly associated with duodenal ulcer (p<0.0001). Conclusion: This study demonstrated relatively high prevalence of H. pylori infection in patients with duodenal ulcer who had undergone upper GI endoscopy.

Download Full-text

Helicobacter pylori cag pathogenicity island genes: clinical relevance for peptic ulcer disease development in Brazil

Journal of Medical Microbiology ◽

10.1099/jmm.0.46824-0 ◽

2007 ◽

Vol 56 (1) ◽

pp. 9-14 ◽

Cited By ~ 30

Author(s):

Rejane Mattar ◽

Sergio Barbosa Marques ◽

Maria do Socorro Monteiro ◽

Anibal Ferreira dos Santos ◽

Kiyoshi Iriya ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Gastric Ulcers ◽

Disease Development ◽

Duodenal Ulcers ◽

Ulcer Disease ◽

Significant Difference ◽

H Pylori ◽

The Right

The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments – cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region – would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from positive urease tests of 150 peptic ulcer patients and 65 dyspeptic controls was analysed by PCR. Duodenal ulcers were present in 110, gastric ulcers in 23 and both gastric and duodenal ulcers in 17 patients. A significant association (P <0.001) was found between a conserved cagPAI and peptic ulcer disease (34 %). The positivity of the cagA gene varied according to the region of the gene that was amplified. The region near to the promoter was present in almost all of the H. pylori isolates (97.2 %). The segment from nt 1764 to 2083 and the extreme right end were frequently deleted in the isolates from the controls (P <0.01). The positivity of the promoter region of cagA and cagT, cagE, cagM and LEC showed a significant difference between the isolates from peptic ulcer patients and from the controls (P <0.01). Patients usually had moderate gastritis; however, the intensity of the active inflammation was higher in the peptic ulcer group (P <0.001). cagT, cagM, LEC and the right end terminus of the cagA-positive H. pylori isolates were associated with a 27-fold, 8-fold, 4-fold and 4-fold risk of peptic ulcer disease, respectively, and may be useful markers to identify individuals at higher risk of peptic ulcer disease development in Brazil.

Download Full-text

A PROSPECTIVE, OBSERVATIONAL STUDY ON THE EFFICACY OF DRUG REGIMEN USED FOR HELICOBACTER PYLORI ERADICATION IN PEPTIC ULCER DISEASE

International Research Journal of Pharmacy ◽

10.7897/2230-8407.1206142 ◽

2021 ◽

Vol 12 (6) ◽

pp. 40-47

Author(s):

Abdullah . ◽

Mounika Pamukuntla

Keyword(s):

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Drug Regimen ◽

The Body ◽

Rapid Urease Test ◽

Medication History ◽

Patient Counselling ◽

Ulcer Disease ◽

Urease Test ◽

H Pylori

Aim of the study: We aimed to study the efficacy of triple drug therapy which is considered as a standard regimen. Objective of the study: To observe the role of pharmacist in preventing the recurrence of H. pylori infection. To assess whether the drug regimen prescribed eradicates the H. pylori infection. Results: In a period of 6 months a total of 358 cases were observed. Among them 142 cases are rapid urease test +ve and 216 are rapid urease test – ve cases. Of 142 patients included in the analysis, Peptic ulcer disease was more common in men when compared to women and prevalent in age group of 51-60 years. The body mass index analysis showed that 35.2% of patients were obese. From the personal history of the patients, it was concluded that 32.3% were smokers whereas 31.6% were alcoholic. The medication history analysis showed non-steroidal anti-inflammatory drugs were commonly used by the patients. The diagnostic endoscopic reports show that patients suffer from erosive pan gastritis followed by erosive gastritis. Conclusion: A 14-day triple therapy was found to be completely eradicate H. pylori infection. Out of the various demographic details that were taken into consideration age, gender, BMI, smokers, alcoholics, spicy food intake and drug abuse increased symptoms whereas co-morbid illness, sleeping pattern and food interval didn’t affect much. Structured patient counselling and follow up had a significant effect which was seen in the form of zero recurrence, 100% medication adherence and improved quality of life.

Download Full-text

Comparison of home made and commercial rapid urease tests for detection of helicobacter pylori in patients with gastroduodenitis and peptic ulcer

Journal of Patan Academy of Health Sciences ◽

10.3126/jpahs.v1i2.16638 ◽

2015 ◽

Vol 1 (2) ◽

pp. 11-14

Author(s):

Yuba Raj Sharma ◽

Amrita Wagley ◽

Sunil Singh

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Predictive Value ◽

Gold Standard ◽

Rapid Urease Test ◽

Ulcer Disease ◽

Urease Test ◽

The Common ◽

H Pylori ◽

Sensitivity Specificity

Introductions: Helicobacter pylori is one of the common and medically prominent infections worldwide and an established etiological factor for peptic ulcer disease. This study was conducted to compare the results of two types of Rapid Urease Tests (RUT) for H. pylori infection.Methods: This study was conducted in patients with gastro duodenal diseases visiting Kantipur Hospital from June to August 2010. Antral biopsies were collected from sixty patients visiting endoscopy unit. The diagnosis was of H. pylori infection carried out using two types of rapid urease tests (commercial and homemade) as well as Histopathology.Results: H. pylori infection was detected in 34 (56.67%) of 60 by histological test, 24 (40%) by homemade kit method and 28 (46.67%) by commercial RUT method. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for RUT (commercial kit) considering histology as gold standard were 76.74%, 92.31%, 92.85% and 75% respectively. The sensitivity, specificity, PPV and NPV for RUT (homemade kit) were 58.82%, 84.62%, 83.3% and 61.11% respectively.Conclusions: Homemade rapid urease test was sensitive and specific for detection of H. pylori infection than commercial test.

Download Full-text