scholarly journals Characterization of some compounds isolated from Sweet Basil (Ocimum basilicum L.) leaf extract

2016 ◽  
Vol 2 (7) ◽  
pp. 159
Author(s):  
Hadush Gebrehiwot ◽  
Aman Dekebo ◽  
Rakesh Kumar Bachheti
Keyword(s):  
Crude Extract ◽  
Ocimum Basilicum ◽  
Spectroscopic Techniques ◽  
Traditional Medicines ◽  
Sweet Basil ◽  
H Nmr ◽  
The Family ◽  
Pure Compounds ◽  
Chloroform Methanol

<p class="abstract"><strong>Background: </strong>For thousands of years, several people depend on traditional medicines from flowers, bark, leaves and fruits of plants. Sweet Basil (<em>Ocimum basilicum L.</em>) is a medicinal herb which belongs to the family of Labiatae and it is the most abundant of the genus <em>Ocimum.</em> The present study aimed to isolate pure compounds from chloroform/methanol (1:1) crude extract of the leaves of <em>O. basilicum </em>herb and characterize them using infra-red (IR) and nuclear magnetic resonance (NMR) spectroscopic techniques.</p><p class="abstract"><strong>Methods:</strong> The sample of powdered leaves of <em>O. basilicum </em>was extracted using a mixture of chloroform and methanol (1:1) and the crude extract was investigated for its chemical composition using spectroscopic techniques.</p><p class="abstract"><strong>Results:</strong> Only one fraction designated as <em>O. basilicum </em>-14 (OB-14) displayed a single spot on TLC plate. Results showed that two isomeric compounds named <em>(1-(2-vinylcyclohexa-1,4-dienyl) propan-2-ol and 2-(2-vinylcyclohexa-1,5-dienyl) propan-1-ol) </em>were isolated as a mixtures using column chromatography over silica gel. The structures of these compounds were identified using IR and one dimensional NMR spectroscopic techniques such as proton NMR (<sup>1</sup>H NMR), carbon-13 NMR (<sup>13</sup>C NMR) and distortionless enhancement by polarization transfer (DEPT).</p><p class="abstract"><strong>Conclusions:</strong> So, more compounds can be isolated from the plant using different chromatographic techniques.</p>

THE IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS FROM ETHYL ACETATE FRACTION OF Dendropthoe falcata

2019 ◽  
Vol 24 (1) ◽  
pp. 13-20
Author(s):  
Astuti Lestari ◽  
Sri Atun
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Melia Azedarach ◽  
Pure Compound ◽  
Plant Parasite ◽  
Pure Compounds ◽  
Two Stages ◽  
Layer Chromatography ◽  
Chloroform Methanol

This study was aimed at identifying secondary metabolites of ethyl acetate fraction of Dendrophtoe falcata (L.f.) Ettingsh mindi plant parasite (Melia azedarach L.). This research was conducted by maceration method using ethanol solvent, partitioning sequentially with n-hexane, chloroform, and ethyl acetate. Ethyl acetate fraction was separated by gravity column chromatography (GCC) in two stages. Phase I GCC used n-hexane : ethyl acetate (9 : 1). Phase II GCC used chloroform : methanol (9 : 1) eluent to obtain one pure compound. Purity identification used thin layer chromatography. Characterization of pure compounds obtained was carried out using UV-Vis and IR. The results show that the isolated compounds maximum wavelengths are at 351.20, 262.60, and 207.20 nm which corresponded to the conjugated synamoyl, benzoyl and chromophore phenol. IR spectrum data shows the presence of O-H, C-H aliphatic, C = O carbonyl, C = C aromatic, and C-O. From these data, the isolated compounds show flavonoid type flavanols.IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DARI FRAKSI ETIL ASETAT BATANG Dendropthoe falcata.Penelitian ini bertujuan untuk mengidentifikasi senyawa metabolit sekunder dari fraksi etil asetat batang Dendrophtoe falcata (L.f.) Ettingsh parasit tumbuhan mindi (Melia azedarach L.). Penelitian ini dilakukan dengan metode maserasi menggunakan pelarut etanol, partisi secara berurutan dengan n-heksana, kloroform, dan etil asetat. Fraksi etil asetat dipisahkan secara kromatografi kolom gravitasi (KKG) dalam dua tahap. KKG tahap I menggunakan eluen n-heksana : etil asetat (9 : 1). KKG tahap II menggunakan eluen kloroform : metanol (9 : 1) sehingga diperoleh satu senyawa murni. Identifikasi kemurnian menggunakan kromatografi lapis tipis. Karakterisasi senyawa murni yang diperoleh dilakukan menggunakan UV-Vis dan IR. Berdasarkan hasil analisis dengan spektrofotometer UV-Vis, senyawa hasil isolasi menunjukkan panjang gelombang maksimum pada 351,20; 262,60; dan 207,20 nm yang sesuai dengan gugus sinamoil, benzoil, dan kromofor fenol terkonjugasi. Data spektrum IR menunjukkan adanya ikatan O-H, C-H alifatik, C=O karbonil, C=C aromatik, dan C-O. Dari data tersebut, senyawa hasil isolasi menunjukkan golongan flavonoid jenis flavonol.

Synthesis and Characterization of Imidazo[1,2-a]Pyrimidine

2015 ◽  
Vol 52 ◽  
pp. 1-4
Author(s):  
A.M. Shah ◽  
A.J. Rojivadiya
Keyword(s):  
Mass Spectroscopy ◽  
Synthesis And Characterization ◽  
Spectroscopic Techniques ◽  
One Pot ◽  
H Nmr ◽  
Ft Ir ◽  
C Nmr

An efficient protocol for the synthesis of imidazo [1,2-a] pyrimidine was developed by using three component one-pot Biginelli synthesis. The synthesized compounds were characterized by spectroscopic techniques like FT-IR, 1H NMR, 13C NMR, and mass spectroscopy.

Photophysical and photochemical properties of fluoroether-substituted zinc(II) and titanium(IV) phthalocyanines

2018 ◽  
Vol 22 (01n03) ◽  
pp. 46-55 ◽  
Author(s):  
İlke Gürol ◽  
Gülay Gümüş ◽  
Deniz Kutlu Tarakci ◽  
Ömer Güngör ◽  
Mahmut Durmuş ◽  
...  
Keyword(s):  
Quantum Yields ◽  
Central Metal Atom ◽  
Spectroscopic Techniques ◽  
Central Metal ◽  
Oxygen Generation ◽  
Fluorescence Quantum Yields ◽  
H Nmr ◽  
Photochemical Properties ◽  
First Time

The synthesis and characterization of novel zinc(II) (1a–4a) and oxo-titanium(IV) (1b–4b) phthalocyanine derivatives bearing 1H,1H-nona?uoro-3,6-dioxaheptan-1-ol groups are described for the first time. These phthalocyanines (1a–4a and 1b–4b) were characterized by elemental analysis and different spectroscopic techniques such as UV-vis, [Formula: see text]H NMR, FTIR and mass. Furthermore, the photophysical (fluorescence quantum yields and lifetimes) and photochemical (singlet oxygen generation and photodegradation) properties of these phthalocyanines were investigated in tetrahydrofuran (THF) solution. The influence of the number of the substituted groups (tetra or octa), position of the substituents (peripheral or non-peripheral) and central metal atom (zinc or titanium) on the photophysical and photochemical properties of these phthalocyanines were evaluated.

scholarly journals Electrochemical and Spectroscopic Characterization of Aluminium(III)-para-methyl-meso-tetraphenylporphyrin Complexes Containing Substituted Salicylates as Axial Ligands

2013 ◽  
Vol 2013 ◽  
pp. 1-10
Author(s):  
Gauri D. Bajju ◽  
Deepmala ◽  
Sunil Kumar Anand ◽  
Sujata Kundan ◽  
Narinder Singh
Keyword(s):  
Spectroscopic Characterization ◽  
Redox Properties ◽  
Proton Nuclear Magnetic Resonance ◽  
Spectroscopic Techniques ◽  
Axial Coordination ◽  
H Nmr ◽  
Axial Ligands ◽  
Reduction Mechanisms ◽  
C Nmr

A series of aluminium(III)-p-methyl-meso-tetraphenylporphyrin (p-CH3TPP-Al(III)) containing axially coordinated salicylate anion [p-CH3TPP-Al-X)], where X = salicylate (SA), 4-chlorosalicylate (4-CSA), 5-chlorosalicylate (5-CSA), 5-flourosalicylate (5-FSA), 4-aminosalicylate (4-ASA), 5-aminosalicylate (5-ASA), 5-nitrosalicylate (5-NSA), and 5-sulfosalicylate (5-SSA), have been synthesized and characterized by various spectroscopic techniques including ultraviolet-visible (UV-vis), infrared (IR) spectroscopy, proton nuclear magnetic resonance (1H NMR) spectroscopy,13C NMR, and elemental analysis. A detailed study of electrochemistry of all the synthesized compounds has been done to compare their oxidation and reduction mechanisms and to explain the effect of axial coordination on their redox properties.

scholarly journals Synthesis and Spectroscopic Characterization of Lead(II)bis{O,O'-ditolyldithiophosphates} and their Adducts with 2,2'-Bipyridyl and 1,10-Phenanthroline

2011 ◽  
Vol 8 (s1) ◽  
pp. S113-S118
Author(s):  
Disha Jain Alok Chaturvedi ◽  
R.K. Upadhayay
Keyword(s):  
Spectroscopic Characterization ◽  
Spectroscopic Studies ◽  
Ammonium Salts ◽  
Molar Ratio ◽  
Spectroscopic Techniques ◽  
Lead Atom ◽  
H Nmr

The reaction of lead(II) dichloride with ammonium salts ofO,O'-ditolyldithiophosphoric acid have been carried out in 1:2 molar ratio by refluxing in benzene. These compounds have been further used to synthesize the adduct of the type lead(II)bis{O,O'-ditolyldithiophosphates}.2,2'-bipyridyl and lead(II)bis {O,O'-ditolyldithiophosphates}.1,10-phenanthroline by the reaction of lead(II)bis{O,O'-ditolyldithiophosphates} and 2,2'-bipyridyl and 1,10-phenanthro-line in the presence of unimolar dichloromethane and ethanol. All these complexes have been characterized by spectroscopic techniques such as IR,1H NMR and31P NMR. On the basis of spectroscopic studies tetracoordinated nature of lead atom in ditolyldithiophosphates complexes and hexacoordinated nature in their adducts have been established.

Isolation and Characterization of the Radiation-Induced Degradation Products of 2′-Deoxyguanosine in Oxygen-Free Aqueous Solutions

1985 ◽  
Vol 40 (11) ◽  
pp. 1519-1531 ◽  
Author(s):  
Maurice Berger ◽  
Jean Cadet
Keyword(s):  
High Performance ◽  
Degradation Products ◽  
Imidazole Ring ◽  
Spectroscopic Techniques ◽  
Decomposition Products ◽  
H Nmr ◽  
Isolation And Characterization ◽  
Radiation Induced ◽  
Main Decomposition

The bulk of the radiation-induced degradation products of 2′-deoxyguanosine in oxygen-free aqueous solution have been separated by high performance liquid chromatography and charac­terized by various spectroscopic techniques including fast atom bombardment mass spectrometry, 1H NMR and circular dichroism. The two main decomposition products result from the opening of the imidazole ring and further rearrangement of the sugar moiety. In addition, the formation of six other nucleosides was shown to involve sugar radicals with subsequent epimerization, re­arrangement or cyclization reactions.

Isolation and Characterization of the Roots of Rumex nervosus

2020 ◽  
Author(s):  
Gashaw Nigussie
Keyword(s):  
Petroleum Ether ◽  
Preparative Thin Layer Chromatography ◽  
Reduced Pressure ◽  
H Nmr ◽  
Isolation And Characterization ◽  
The Family ◽  
Exhaustive Extraction ◽  
Layer Chromatography ◽  
C Nmr

Abstract Rumex nervosus belongs to the family of Polygonaceae,which is traditionally used in Ethiopia to treat various diseases. This prompted us to isolate bioactive compounds from the root of this plant. Ground root parts of Rumex nervosus were subjected to exhaustive extraction successively with petroleum ether and methanol.The solvent from each extract was evaporated under reduced pressure using rotavapour to obtain petroleum ether and methanol extract. Chromatographic purification of the methanol extracts by Column chromatography followed by Preparative Thin layer Chromatography using Chloroform: methanol (9.5:0.5) ratio gave a compound coded as RN-6. The structure of this compound 4-ethylheptyl benzoate was characterized as by means of 1H NMR, 13C NMR, UV and IR spectral data.

scholarly journals Synthesis and Evaluation of Changes Induced by Solvent and Substituent in Electronic Absorption Spectra of New Azo Disperse Dyes Containig Barbiturate Ring

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hooshang Hamidian ◽  
Najmeh Zahedian ◽  
Dadkhoda Ghazanfari ◽  
Samieh Fozooni
Keyword(s):  
Aromatic Aldehyde ◽  
Electronic Absorption Spectra ◽  
Thiobarbituric Acid ◽  
Disperse Dyes ◽  
Spectroscopic Techniques ◽  
H Nmr ◽  
Ft Ir ◽  
Azo Disperse Dyes ◽  
C Nmr

Six azo disperse dyes were prepared by diazotizing 4-amino hippuric acid and coupled with barbituric acid and 2-thiobarbituric acid. Then, the products were reacted with aromatic aldehyde, sodium acetate, and acetic anhydride, and oxazolone derivatives were formed. Characterization of the dyes was carried out by using UV-Vis, FT-IR,1H NMR and13C NMR, and mass spectroscopic techniques. The solvatochromic behavior of azo disperse dyes was evaluated in various solvents. The effects of substituents of aromatic aldehyde, barbiturate, and thiobarbiturate ring on the color of dyes were investigated.

scholarly journals Isolation and Characterization of the Roots of Rumex nervosus

2020 ◽  
Vol 5 (1) ◽  
pp. 39-50
Author(s):  
Gashaw Nigussie
Keyword(s):  
Petroleum Ether ◽  
Preparative Thin Layer Chromatography ◽  
Chromatographic Purification ◽  
Reduced Pressure ◽  
Isolation And Characterization ◽  
The Family ◽  
Exhaustive Extraction ◽  
Layer Chromatography ◽  
Chloroform Methanol

Rumex nervosus belongs to the family of Polygonaceae,which is traditionally used in Ethiopia to treat various diseases. This prompted us to isolate bioactive compounds from the root of this plant. Ground root parts of Rumex nervosus were subjected to exhaustive extraction successively with petroleum ether and methanol.The solvent from each extract was evaporated under reduced pressure using rotavapour to obtain petroleum ether and methanol extract. Chromatographic purification of the methanol extracts by Column chromatography followed by Preparative Thin layer Chromatography using Chloroform: methanol (9.5:0.5) ratio gave a compound coded as RN-6. The structure of this compound 4-ethylheptyl benzoate was characterized as by means of 1H NMR, 13C NMR, UV and IR spectral data.

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