THE IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS FROM ETHYL ACETATE FRACTION OF Dendropthoe falcata

2019 ◽  
Vol 24 (1) ◽  
pp. 13-20
Author(s):  
Astuti Lestari ◽  
Sri Atun
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Melia Azedarach ◽  
Pure Compound ◽  
Plant Parasite ◽  
Pure Compounds ◽  
Two Stages ◽  
Layer Chromatography ◽  
Chloroform Methanol

This study was aimed at identifying secondary metabolites of ethyl acetate fraction of Dendrophtoe falcata (L.f.) Ettingsh mindi plant parasite (Melia azedarach L.). This research was conducted by maceration method using ethanol solvent, partitioning sequentially with n-hexane, chloroform, and ethyl acetate. Ethyl acetate fraction was separated by gravity column chromatography (GCC) in two stages. Phase I GCC used n-hexane : ethyl acetate (9 : 1). Phase II GCC used chloroform : methanol (9 : 1) eluent to obtain one pure compound. Purity identification used thin layer chromatography. Characterization of pure compounds obtained was carried out using UV-Vis and IR. The results show that the isolated compounds maximum wavelengths are at 351.20, 262.60, and 207.20 nm which corresponded to the conjugated synamoyl, benzoyl and chromophore phenol. IR spectrum data shows the presence of O-H, C-H aliphatic, C = O carbonyl, C = C aromatic, and C-O. From these data, the isolated compounds show flavonoid type flavanols.IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DARI FRAKSI ETIL ASETAT BATANG Dendropthoe falcata.Penelitian ini bertujuan untuk mengidentifikasi senyawa metabolit sekunder dari fraksi etil asetat batang Dendrophtoe falcata (L.f.) Ettingsh parasit tumbuhan mindi (Melia azedarach L.). Penelitian ini dilakukan dengan metode maserasi menggunakan pelarut etanol, partisi secara berurutan dengan n-heksana, kloroform, dan etil asetat. Fraksi etil asetat dipisahkan secara kromatografi kolom gravitasi (KKG) dalam dua tahap. KKG tahap I menggunakan eluen n-heksana : etil asetat (9 : 1). KKG tahap II menggunakan eluen kloroform : metanol (9 : 1) sehingga diperoleh satu senyawa murni. Identifikasi kemurnian menggunakan kromatografi lapis tipis. Karakterisasi senyawa murni yang diperoleh dilakukan menggunakan UV-Vis dan IR. Berdasarkan hasil analisis dengan spektrofotometer UV-Vis, senyawa hasil isolasi menunjukkan panjang gelombang maksimum pada 351,20; 262,60; dan 207,20 nm yang sesuai dengan gugus sinamoil, benzoil, dan kromofor fenol terkonjugasi. Data spektrum IR menunjukkan adanya ikatan O-H, C-H alifatik, C=O karbonil, C=C aromatik, dan C-O. Dari data tersebut, senyawa hasil isolasi menunjukkan golongan flavonoid jenis flavonol.

scholarly journals ISOLATION AND CHARACTERIZATION OF TERPENOID COMPOUNDS FROM LEAVES OF Macaranga beccariana Merr.

2018 ◽  
Vol 16 (1) ◽  
pp. 22 ◽  
Author(s):  
Doni Prio Atmoko ◽  
Eva Marliana ◽  
Erwin Erwin
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Gradient Elution ◽  
Isolation And Characterization ◽  
Rf Values ◽  
Ft Ir ◽  
Elution Method ◽  
Layer Chromatography ◽  
Chloroform Methanol

Isolation and characterization of terpenoid compounds from ethyl acetate fraction of Macaranga beccariana Merr. have been done. The separation process at ethyl acetate fraction using gravity column chromatography with gradient elution method. The obtained isolates were tested for purity using thin layer chromatography with various eluents namely n-hexane, chloroform, methanol, acetone and ethyl acetate with Rf values 0; 0.75; 0.80; 0.87 and 0.97 respectively. The terpenoids which were successfully isolated were white crystals with a mass of 0.0119 grams. UV analysis produced 1 peak at l 207.03 nm. Analysis of compounds with FT-IR indicated the functional groups O-H, aliphatic C-H, aliphatic C=C, C-O and C=O. Based on the results of the UV and FT-IR spectrum, the isolate compounds were thought to contain terpenoids.

scholarly journals Isolation of Four Flavanones from the Leaves of Macaranga Hypoleuca (Rchb.f. & Zoll.) Müll.Arg

2019 ◽  
Vol 7 (4.14) ◽  
pp. 206
Author(s):  
H. Muhamad Nazeri ◽  
N. Ahmat ◽  
K. Abdul Khalil
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Tropical Region ◽  
Pure Compounds ◽  
Large Genus ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

Macaranga is a large genus belongs to Euphorbiaceae family which commonly distributed in the tropical region of Africa, South-East Asia, China, and India. The plant of this genus contain flavonoids and stilbenes bearing various terpenyl groups including prenyl, geranyl and farnesyl groups. The leaves of M. hypoleuca were collected from reserved forest UiTM Jengka Pahang, Malaysia, and the dried powdered leaves were macerated in methanol at room temperature. The crude methanol extract was subjected to liquid-liquid partition using n-hexane and ethyl acetate to obtain hexane, ethyl acetate and aqueous fractions. The ethyl acetate fraction was semi purified using vacuum liquid chromatography (VLC) to give nine major fractions (MHL1-MHL9). Fraction MHL3 was further purified by column chromatography (CC) and preparative thin layer chromatography (p-TLC) to give two pure compounds, 8-prenylnaringenin (1) and sakuranetin (5,4’-dihydroxy-7-methoxyflavanone) (3). Meanwhile, fraction MHL6 was purified using column chromatography (CC) and p-TLC to yield another two pure compounds, 6-(3-hydroxy-3-methyl)naringenin (2) and 7-O-methyleriodictyol (4). The chemical structure of these isolated compounds were determined based on their 1D and 2D NMR, UV, and IR data. From this study, four flavanones were isolated from the leaves of M. hypoleuca.  

scholarly journals Molecular Characterization of Penicillium expansum Isolated from Grapes and its Management by Leaf Extract of Chenopodium murale

2021 ◽  
Vol 10 (1) ◽  
pp. ACCEPTED
Author(s):  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Syeda Fakehha Naqvi
Keyword(s):  
Ethyl Acetate ◽  
Fungal Pathogen ◽  
Leaf Extract ◽  
Fruits And Vegetables ◽  
Ethyl Acetate Fraction ◽  
Penicillium Expansum ◽  
Blue Mold ◽  
Chenopodium Murale

Penicillium expansum Link causes an economically important postharvest blue mold disease in a number of fruits and vegetables. In the present study, this fungus was isolated from rotted grapes and identified on morphological basis. Identification of the pathogen was further confirmed on molecular basis by using four different primer pairs namely ITS, β-tubulin, CMD and CF under accession numbers MN752155, MN787831, MN787832 and MN787833, respectively. Leaf extract of Chenopodium murale was assessed for its potential to control in vitro growth of P. expansum. For this purpose, leaves were extracted in methanol and after evaporation of the solvent, the resulting extract was successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol followed by antifungal bioassays with different concentrations (1.562 to 200 mg mL-1) each organic solvent fraction. Although all the fractions variably controlled the fungal pathogen, however, n-butanol fraction showed the highest antifungal activity causing 45–86% reduction in biomass of the pathogen. Ethyl acetate fraction was also highly antifungal and reduced fungal biomass by 44–81%. Chloroform and n-hexane fractions were comparatively less effective and reduced biomass of P. expansum by 30–72% and 11–44%, respectively. This study concludes that ethyl acetate and n-butanol fractions are highly antifungal in nature against P. expansum.

scholarly journals Characterization of some compounds isolated from Sweet Basil (Ocimum basilicum L.) leaf extract

2016 ◽  
Vol 2 (7) ◽  
pp. 159
Author(s):  
Hadush Gebrehiwot ◽  
Aman Dekebo ◽  
Rakesh Kumar Bachheti
Keyword(s):  
Crude Extract ◽  
Ocimum Basilicum ◽  
Spectroscopic Techniques ◽  
Traditional Medicines ◽  
Sweet Basil ◽  
H Nmr ◽  
The Family ◽  
Pure Compounds ◽  
Chloroform Methanol

<p class="abstract"><strong>Background: </strong>For thousands of years, several people depend on traditional medicines from flowers, bark, leaves and fruits of plants. Sweet Basil (<em>Ocimum basilicum L.</em>) is a medicinal herb which belongs to the family of Labiatae and it is the most abundant of the genus <em>Ocimum.</em> The present study aimed to isolate pure compounds from chloroform/methanol (1:1) crude extract of the leaves of <em>O. basilicum </em>herb and characterize them using infra-red (IR) and nuclear magnetic resonance (NMR) spectroscopic techniques.</p><p class="abstract"><strong>Methods:</strong> The sample of powdered leaves of <em>O. basilicum </em>was extracted using a mixture of chloroform and methanol (1:1) and the crude extract was investigated for its chemical composition using spectroscopic techniques.</p><p class="abstract"><strong>Results:</strong> Only one fraction designated as <em>O. basilicum </em>-14 (OB-14) displayed a single spot on TLC plate. Results showed that two isomeric compounds named <em>(1-(2-vinylcyclohexa-1,4-dienyl) propan-2-ol and 2-(2-vinylcyclohexa-1,5-dienyl) propan-1-ol) </em>were isolated as a mixtures using column chromatography over silica gel. The structures of these compounds were identified using IR and one dimensional NMR spectroscopic techniques such as proton NMR (<sup>1</sup>H NMR), carbon-13 NMR (<sup>13</sup>C NMR) and distortionless enhancement by polarization transfer (DEPT).</p><p class="abstract"><strong>Conclusions:</strong> So, more compounds can be isolated from the plant using different chromatographic techniques.</p>

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

TLC-Bioautography Profile of Ethyl Acetate Extract of 5 Bacteria Isolated from Ficus carica L Rhizosphere

2015 ◽  
Vol 4 (2) ◽  
pp. 81
Author(s):  
Iin Narwanti ◽  
Nanik Sulistyani
Keyword(s):  
Ethyl Acetate ◽  
Room Temperature ◽  
Solvent System ◽  
Ficus Carica ◽  
Broth Culture ◽  
Nrps Gene ◽  
E Coli ◽  
Gene Profiles ◽  
Layer Chromatography ◽  
Chloroform Methanol

Research has been conducted on the 5 isolates of bacteria isolated from the rhizosphere of Ficus carica L as a producer of antibiotics. The previous study showed they have NRPS gene profiles that differ from each other. This study aims to determine the TLC Rf spots having inhibition activity against the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The study was conducted using the 5 bacteria isolates, namely T19, T24, T25, T37 and T41. All isolates were fermented at room temperature for 14 days. Further the each broth culture was filtered and extracted using ethyl acetate. Components in the extract were separated by thin layer chromatography (TLC) with the mobile phase of chloroform-methanol (7: 3), followed by bioautography test against the S. aureus and E. coli to determine the chromatogram spots containing antibiotics. TLC results showed all isolates had different chromatogram profiles. The bioautography results showed that only isolate T25 can produce antibiotics against S. aureus. The antibiotic spot was at Rf 0.9 in the use of chloroform-methanol (7: 3) solvent system.

CYTOTOXIC COMPOUND FROM ETHYL ACETATE FRACTION OF Brucea javanica (L.) Merr FRUIT AGAINST HeLa CELL LINE

2015 ◽  
Vol 11 (1) ◽  
pp. 58
Author(s):  
Nurlina Nurlina ◽  
Ari Widiyantoro
Keyword(s):  
Ethyl Acetate ◽  
Amorphous Solid ◽  
Ethyl Acetate Fraction ◽  
Assay Method ◽  
Pure Compound ◽  
Brucea Javanica ◽  
H Nmr ◽  
Cytotoxic Compound ◽  
Related Compounds ◽  
C Nmr

<p>The pure compound relatively (85 mg) was isolated from 4.5 kg <em>Brucea javanica</em> (L.) Merr. fruits sample. It was obtained from ethyl acetate fraction as a red-brownish amorphous solid of melting point 202-206 <sup>o</sup>C. Phytochemical screening of isolate showed positive result of terpenoid group. Based on analysis by UV, IR,<sup>1</sup>H-NMR and <sup>13</sup>C-NMR spectroscopy and by comparison with known related compounds, indicated that isolate is quassinoid compound named Picras 3- en- 21- oat acid, 15-(acetyloxy)-13,20- epoxy-3-(ß-D- glucopyranosyloxy)-11, 12- dihydroxy -2, 16- dioxo -, methyl ester. Cytotoxic test using MTT assay method showed that compound have IC<sub>50</sub> 9,7 µg/mL.</p>

scholarly journals Isolasi Senyawa Flavonoid Pada Biji Pinang Yaki (Areca vestiaria Giseke)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 40
Author(s):  
Cindy C. Satolom
Keyword(s):  
Ethyl Acetate ◽  
Uv Spectroscopy ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Compound ◽  
Endemic Plant ◽  
H Nmr ◽  
North Sulawesi ◽  
Nmr Methods ◽  
Layer Chromatography ◽  
Typical Colour

Pinang Yaki (Areca vestiaria Giseke) adalah tanaman endemik Sulawesi Utara yang berkhasiat untuk mengobati berbagai penyakit. Penelitian sebelumnya menyatakan bahwa biji dan kulit Pinang Yaki mengandung flavonoid (Samosir et al., 2012; Mamonto et al., 2014). Sekarang akan dilaporkan Isolasi Senyawa Flavonoid dari biji Pinang Yaki. Metoda yang dilakukan sebagai berikut: Ekstrak metanol difraksinasi dengan n-heksan, etil asetat dan air. Fraksi etil asetat dimurnikan dengan metode Kromatografi Lapis Tipis Kresgel G60 F254 dan Kromatografi Kolom dengan berbagai pelarut, setiap pemisahan dipandu dengan AlCl3 5% dalam etanol yang menampakan warna khas dari flavonoid. Isolat murni yang didapatkan selanjutnya dikarakterisasi dengan menggunakan metode spektroskopi UV dan H-NMR. Isolat murni yang diperoleh diduga adalah Afzelekin.Pinang Yaki (Areca vestiaria Giseke) is a endemic plant of North sulawesi that efficacious for various disease treatment. Same research reported that Pinang Yaki seed and skin seed contain Flavonoid compound (Samosir et al., 2012; Mamonto et al., 2014).  This research reported isolation of Flavonoid compound from Pinang Yaki seed, The methods is: methanol ekstract fractionating with n-hexane, ethyl acetate dan aquades. Ethyl acetate fraction was purified by Thin Layer Chromatography Kresgel G60 F254 method and Column Chromatography with various solvent, every separation guided by 5% AlCl3 in ethanol which reveals the typical colour of Flavonoid compound. After that, the obtained pure isolates was characterized by various UV-Spectroscopy and H-NMR methods. The structure of isolate compound predicted as Afzelechin.

scholarly journals Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

2019 ◽  
pp. 1-10
Author(s):  
Halima A. Umar ◽  
Hamidu Usman ◽  
Mustapha B. Abubakar ◽  
Baba F. Mohammed ◽  
Mohammed Babakura ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanol Extract ◽  
Phyllanthus Amarus ◽  
Hydrolysable Tannin ◽  
Isolation And Characterization ◽  
Hydrolysable Tannins ◽  
Isolated Compound ◽  
Layer Chromatography

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

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