Evaluation of antianxiety properties of Piper betle L. leaf extracts on swiss albino mice

Background: There is a growing demand for alternative medicines derived from indigenous plants having natural antioxidants and neuroprotective actions for the treatment of many behavioural disorders such as anxiety and depression. This study was designed to screen antidepressant activity of aqueous extract of Piper betle L. leaf (betel leaf) in Swiss albino mice.Methods: Swiss albino mice of both sexes weighing 25-30grams were used in the present study. Piper betle leaves aqueous extract (PBAE) was administered to the animals at a dose of 100, 200mg/kg body weight orally for 14 days. On the 14th day, after 1 hour of PBAE administration, experiments on force swim test (FST) and tail suspension (TST) were carried out for studying the level of depression. In FST and TST, time of immobility was noted for a period of 5 minutes.Results: Data was analyzed by one-way ANOVA followed by Tukey Kramer’s multiple comparison test at P = 0.05. The results were represented as Mean±SE. PBAE at a dose of 100mg/kg has shown significant antidepressant activity, as evidenced by decrease in the immobility time in both the screening tests of depression.Conclusions: Present results demonstrated that PBAE possess potent antidepressant property. The exact mechanism(s) related to the active compound(s) in Piper betle leaf extract have to be elucidated in future studies.

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Antiplasmodial and haematological effects of Senna occidentalis leaf ethanolic extracts on Swiss albino mice infected with Plasmodium berghei

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v12i2.17 ◽

2021 ◽

Vol 12 (2) ◽

pp. 122-128

Author(s):

S.M. Babangida ◽

G.J. Sow ◽

D.M. Shehu

Keyword(s):

Plasmodium Berghei ◽

Blood Cells ◽

Leaf Extract ◽

White Blood Cells ◽

Control Group ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Swiss Albino Mice ◽

Ethanolic Extracts ◽

Albino Mice

A research was conducted to investigate the haematological effects of ethanolic leaf extracts of Senna occidentalis on Swiss albino mice infected with 0.2 ml of Plasmodium berghei infected blood. Fifteen (15) mice weighing between 140-260g were assigned into five study groups of three mice each. The first group is treated with 0.2 mL of normal saline (drug free control). Group 2, 3, 4 were treated with 100, 200, and 400mg/kg of theethanolic leaf extract respectively while group 5 received 10mg/kg of chloroquine phosphate. All doses were administered orally. The results obtained were analyzed using Analysis of Variance with Duncan’s Multiple Range Test to separate the means. The result of the preliminary phytochemical analysis revealed the presence of alkaloids, saponins, cardiac glycosides, diterpenoids, flavonoids, steroids, tannins, Triterpenoids, carbohydrates and proteins. The level of parasite suppression ranges from 35% to 75% and the activity increased with increase in concentration of the extracts (dose dependent). The extracts were found to increase the level of some haematological parameters such as Red Blood Cells, White Blood Cells and Haemoglobin. The effect is concentration dependent, increases with increase in concentration. Thus, the anti plasmodial efficacy of the leaf extract of S. occidentalis on P. berghei is confirmed. It is recommended that , 400 mg/kg leaf ethanolic extracts of S. occidentalis couldbe use in the treatment of malarial fever.

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Toxicological Effects, Prophylactic and Curative Activity of Rauwolfia vomitoria Leaf Extracts on Plasmodium Berghei NK 65 infected Swiss albino mice

American Journal of Biomedical Science & Research ◽

10.34297/ajbsr.2019.03.000730 ◽

2019 ◽

Vol 3 (6) ◽

pp. 522-528

Author(s):

Omoya Funmilola Oluyemi

Keyword(s):

Plasmodium Berghei ◽

Leaf Extracts ◽

Swiss Albino Mice ◽

Toxicological Effects ◽

Albino Mice ◽

Rauwolfia Vomitoria

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Effect of Piper Betle Leaf Extracts on Enhancement of Memory and Learning in Rodents

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/591 ◽

2021 ◽

Vol 10 (34) ◽

pp. 2900-2904

Author(s):

Roopa Prasad Nayak ◽

Uttara Krishna ◽

Chaitra S.R.

Keyword(s):

Learning And Memory ◽

Aqueous Extract ◽

Elevated Plus Maze ◽

Ethanolic Extract ◽

Screening Tests ◽

Piper Betle ◽

Swiss Albino Mice ◽

Plus Maze ◽

Albino Mice ◽

Memory Enhancing

BACKGROUND Medicinal plants have long been used for treating various diseases. Piper betle, an evergreen and perennial plant, is valued for its medicinal properties from ancient times. The purpose of this study was to assess the effect of aqueous extract and ethanolic extract of Piper betle leaf on animal models of learning and memory and compare it with piracetam. METHODS Piper betle leaves aqueous extract (PBAE) and ethanolic extract (PBEE) were administered to the Swiss albino mice at a dose of 100, 200 mg/kg body weight orally for 14 days to evaluate their effect on spatial learning and memory using the Hebb– William’s maze (HWM) and elevated plus maze (EPM). The study group included a control (distilled water) and a standard nootropic agent (Piracetam 150 mg/kg). RESULTS One-way analysis of variance (ANOVA) followed by Tukey Kramer’s multiple comparison test (P = 0.05) were used for comparison of groups and tabulated as mean ± SE. PBEE at a dose of 100 mg/kg and 200 mg/kg has shown significant memory enhancing activity, as indicated by a decrease in the TRC (time taken to reach the reward chamber) and TL (transfer latency) in both the screening tests of learning and memory. CONCLUSIONS PBEE possess potent memory enhancing property and can be considered as a potential drug for improving memory in the medical management of dementia, Alzheimer’s disease, and neurodegenerative disorders. KEY WORDS Piper betle L, Swiss Albino Mice, Hebb–Williams Maze (HWM), Elevated Plus Maze (EPM), Piracetam.

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