scholarly journals Efficacy of rhNGF-loaded amniotic membrane transplantation for rabbit corneal epithelial and nerve regeneration

2021 ◽  
Vol 14 (11) ◽  
pp. 1653-1659
Author(s):  
Lu-Qin Wan ◽  
◽  
Hui-Feng Wang ◽  
Chen Chen ◽  
Hua Li ◽  
...  
Keyword(s):  
Sustained Release ◽  
Nerve Regeneration ◽  
Nerve Fiber ◽  
Amniotic Membrane ◽  
Control Group ◽  
Epithelial Defect ◽  
Corneal Sensitivity ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect

AIM: To evaluate the efficacy of recombinant human nerve growth factor-loaded amniotic membrane (rhNGF-AM) on corneal epithelial and nerve regeneration in rabbit model. METHODS: Freshly prepared human amniotic membrane (AM) were immersed into PBS buffer containing 100 or 500 μg/mL rhNGF for 15, 30, and 60min at 4℃. The in vitro release kinetics of rhNGF was measured with ELISA. For in vivo evaluation, the AM were immersed with 500 μg/mL rhNGF for 30min. Fifty-seven rabbits were selected to establish corneal epithelial defect model. In addition to the 19 rabbits in control group, 38 rabbits received AM transplantation with or without rhNGF after the removal of central epithelium. Corneal epithelial defect area, sub-epithelial nerve fiber density, corneal sensitivity, rhNGF contents in resident AM and corneas were measured after the surgery. RESULTS: rhNGF was sustained release from the AM within 14d in vitro, with the positive correlation with initial immersion concentration. The immersion of AM in 500 μg/mL rhNGF for 30min achieved the most stable release within 14d. After transplantation in rabbit cornea, a high concentration of rhNGF in resident rhNGF-AM and cornea was maintained within 8d. Corneal epithelial healing, nerve fiber regeneration and the recovery of corneal sensitivity were significantly accelerated after the rhNGF-AM transplantation when compared to simple AM transplantation (all P<0.05). CONCLUSION: Simple immersion of AM achieves the sustained release of rhNGF, and promotes corneal epithelial wound healing and nerve regeneration, as well as the recovery of corneal sensitivity in rabbit.

scholarly journals Subconjunctival Injection of Transdifferentiated Oral Mucosal Epithelial Cells for Limbal Stem Cell Deficiency in Rats

2020 ◽  
pp. 002215542098007
Author(s):  
Yu-Ting Xiao ◽  
Hua-Tao Xie ◽  
Xin Liu ◽  
Chao-Ye Duan ◽  
Jing-Yu Qu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Pigment Epithelium ◽  
Limbal Stem Cell Deficiency ◽  
Subconjunctival Injection ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Limbal Stem Cell

Rat limbal niche cells (LNCs) have been proven to induce transdifferentiation of oral mucosal epithelial cells (OMECs) into corneal epithelial-like cells termed transdifferentiated oral mucosal epithelial cells (T-OMECs). This investigation aimed to evaluate the effect of subconjunctival T-OMEC injections on alkali-induced limbal stem cell deficiency (LSCD) in rats. LNCs were cocultured with OMECs in the Transwell system to obtain T-OMECs, with NIH-3T3 cells serving as a control. Subconjunctival injection of single T-OMEC or OMEC suspension was performed immediately after corneal alkali injury. T-OMECs were prelabeled with the fluorescent dye CM-DiI in vitro and tracked in vivo. Corneal epithelial defect, opacity, and neovascularization were quantitatively analyzed. The degree of corneal epithelial defect (from day 1 onward), opacity (from day 5 onward), and neovascularization (from day 2 onward) was significantly less in the T-OMEC group than in the OMEC group. Cytokeratin 12 (CK12), pigment epithelium–derived factor, and soluble fms-like tyrosine kinase-1 were expressed at a higher rate following T-OMEC injection. Some CM-DiI-labeled cells were found to be coexpressed with CK12, Pax6, and ΔNp63α in the corneal epithelium after subconjunctival injection. Subconjunctival injection of T-OMECs prevents conjunctival invasion and maintains a normal corneal phenotype, which might be a novel strategy in the treatment of LSCD:

scholarly journals Outcome of Amniotic Membrane Transplant in Persistent Corneal Epithelial Defects

2021 ◽  
Vol 38 (1) ◽  
Author(s):  
Tiabbah Saleem ◽  
Syeda Ayesha Bokhari
Keyword(s):  
Amniotic Membrane ◽  
Case Series ◽  
Bullous Keratopathy ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Case Series Study ◽  
Chemical Injuries ◽  
Standard Medical Treatment ◽  
Disease Study

Purpose:  To determine the outcome of amniotic membrane transplant in patients with ocular surface disease. Study Design: Interventional Case series study Place and Duration of Study:  Layton Rahmatullah Benevolent Trust Eye Hospital, Korangi 2½, Karachi, from January, 2019 to July 2019. Methods:  Patients with an age range of 20–45 years, either gender and who had persistent corneal epithelial defects as a consequence of keratitis, chemical injuries, bullous keratopathy and Mooren’s ulcers were included. Informed consent was obtained. Preserved amniotic membrane was used in all patients and they were followed at 1 week, 1, 3 and finally at 6 months and results were evaluated in terms of stable, healed and pain-free cornea. Results:  Mean age of the patients was 37.94 ± 6.78 years. Majority of the patients (60.0%) were less than 40 years of age. Out of 58 patients, 45 (77.6%) were males and 13 (22.4%) were females. Mean duration of injury was 7.67 ± 2.28 weeks with majority of patients i.e. 62% were with less than 8 weeks of duration. Most common cause of persistent corneal epithelial defect was bacterial keratitis (17.2%) followed by vegetative trauma (13.8%). Successful surgical outcome as healed cornea was observed in 53 (91.4%) patients. Conclusion:  Amniotic membrane transplant is an effective treatment for persistent corneal epithelial defects unresponsive to standard medical treatment. This surgical technique is effective in terms of healing and resolving the inflammation and preserving the useful vision. Key Words:  Persistant corneal epithelial defect, Amniotic Membrane, Bullous keratopathy.

scholarly journals Heparin-Modified Amniotic Membrane Combined With Growth Factors for Promoting Corneal Wound Healing After Alkali Burn

2020 ◽  
Vol 8 ◽  
Author(s):  
Xuan Zhao ◽  
Xin Zuo ◽  
Jing Zhong ◽  
Bowen Wang ◽  
Saiqun Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Sustained Release ◽  
Corneal Epithelial Cell ◽  
Control Group ◽  
Therapeutic Effects ◽  
Cell Growth And Migration ◽  
Corneal Epithelial

Ocular chemical burns are potentially blinding ocular injuries and require urgent management. Amniotic membrane (AM) transplantation is an effective surgical treatment, one of the reasons is because AM is a rich source of growth factors that can promote epithelialization and wound healing. However, growth factors will be gradually lost and insufficient after preparation process and long-time storage, leading to unsatisfactory therapeutic effects. Herein, we present a modified AM (AM-HEP) for the supplement and sustained release of growth factor by surface grafting heparin for treatment of ocular chemical burns. Heparin grafting rate and stability, microstructure, physical property, and sustained release of epithelial growth factor (EGF) of AM-HEP were characterized. Biocompatibility and ability to promote corneal epithelial cell growth and migration were evaluated and compared with a biological amnion, which is available on the market in vitro. The therapeutic effects of AM-HEP combined with EGF (AM-HEP@EGF) in vivo had been evaluated in a model of mouse corneal alkali burn. The results indicated that heparin was introduced into AM and maintain stability over 3 weeks at 37°C. The modification process of AM-HEP did not affect microstructure and physical property after comparing with non-modified AM. EGF could be combined quickly and effectively with AM-HEP; the sustained release could last for more than 14 days. AM-HEP@EGF could significantly promote corneal epithelial cell growth and migration, compared with non-modified AM and control group. Faster corneal epithelialization was observed with the transplantation of AM-HEP@EGF in vivo, compared with the untreated control group. The corneas in the AM-HEP@EGF group have less inflammation and were more transparent than those in the control group. The results from in vitro and in vivo experiments demonstrated that AM-HEP@EGF could significantly enhance the therapeutic effects. Taken together, AM-HEP@EGF is exhibited to be a potent clinical application in corneal alkali burns through accelerating corneal epithelial wound healing.

Detection of Corneal Epithelial Defect Through Amniotic Membrane Patch by Fluorescein

Cornea ◽  
2005 ◽  
Vol 24 (3) ◽  
pp. 359-360 ◽  
Author(s):  
Akira Kobayashi ◽  
Shigeyuki Ijiri ◽  
Kazuhisa Sugiyama ◽  
Mario A Di Pascuale ◽  
Scheffer C. G Tseng
Keyword(s):  
Amniotic Membrane ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Membrane Patch

Corneal Epithelial Defect May Affect Anterior Chamber Flare

1996 ◽  
Vol 12 (7) ◽  
pp. 755-755
Author(s):  
Ran Sun ◽  
Howard V Gimbel
Keyword(s):  
Anterior Chamber ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Anterior Chamber Flare

scholarly journals Cytoprotective effect of amniotic membrane extracts on human corneal epithelial cells exposed to benzalkonium chloride in vitro

2020 ◽  
Vol 5 ◽  
pp. 11-11
Author(s):  
Jiayan Liu ◽  
Huyong Zou ◽  
Minzhi Zeng ◽  
Amy Michelle Huang ◽  
Yan Chen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Benzalkonium Chloride ◽  
Cytoprotective Effect ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells

Severe Corneal Complications Associated With Corneal Lubricant Used During Surgery

2017 ◽  
Vol 1 (3) ◽  
pp. 187-190
Author(s):  
Andrew C. Lee ◽  
E. Mitchel Opremcak ◽  
Christiane Hunt ◽  
Juan A. Astruc ◽  
Brian Toussaint ◽  
...  
Keyword(s):  
Treatment Method ◽  
The United States ◽  
Posterior Segment ◽  
Epithelial Defect ◽  
Chemical Burns ◽  
Corneal Epithelial ◽  
Corneal Injury ◽  
Corneal Epithelial Defect ◽  
Time Period ◽  
Litmus Paper

Purpose: To present a series of cases of corneal chemical burns associated with the use of likely improperly buffered corneal lubricant gel, Goniovisc, during uncomplicated, posterior segment surgery. Methods: Cases of postoperative corneal complications following surgeries in which Goniovisc was used were collected from 6 retina practices across the United States between January and August 2016. The preoperative diagnosis, type of procedure, postoperative day 1 corneal findings, treatment method, and time to resolution of the corneal irregularity were recorded. pH testing of Goniovisc lots from this time period was performed using Hydrion litmus paper. Results: A total of 157 eyes of 156 patients were found to have corneal complications following relatively simple posterior segment surgeries in which Goniovisc was used. Findings included one or more of the following: corneal epithelial defect (84% of cases), Descemet folds (34%), stromal edema (14%), and superficial punctate keratitis (3%). Time to resolution ranged from 2 to 90 days (mean, 12 days; median, 7 days). Treatment methods included artificial tears (37%), bandage contact lens (28%), antibiotic drops (17%), antibiotic ointment (16%), and patching (9%). Two cases were treated with amniotic membrane grafts. One case required a penetrating keratoplasty due to an old laser-assisted in situ keratomileusis (LASIK) flap with a nonhealing epithelial defect that scarred significantly. Litmus testing of Goniovisc lots from this time period revealed a pH of 4 to 5. Conclusion: Chemical burns secondary to improperly buffered corneal lubricant solution should be considered in the differential when corneal injury is encountered following posterior segment surgery.

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