scholarly journals Outcome of Amniotic Membrane Transplant in Persistent Corneal Epithelial Defects

2021 ◽  
Vol 38 (1) ◽  
Author(s):  
Tiabbah Saleem ◽  
Syeda Ayesha Bokhari
Keyword(s):  
Amniotic Membrane ◽  
Case Series ◽  
Bullous Keratopathy ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Case Series Study ◽  
Chemical Injuries ◽  
Standard Medical Treatment ◽  
Disease Study

Purpose:  To determine the outcome of amniotic membrane transplant in patients with ocular surface disease. Study Design: Interventional Case series study Place and Duration of Study:  Layton Rahmatullah Benevolent Trust Eye Hospital, Korangi 2½, Karachi, from January, 2019 to July 2019. Methods:  Patients with an age range of 20–45 years, either gender and who had persistent corneal epithelial defects as a consequence of keratitis, chemical injuries, bullous keratopathy and Mooren’s ulcers were included. Informed consent was obtained. Preserved amniotic membrane was used in all patients and they were followed at 1 week, 1, 3 and finally at 6 months and results were evaluated in terms of stable, healed and pain-free cornea. Results:  Mean age of the patients was 37.94 ± 6.78 years. Majority of the patients (60.0%) were less than 40 years of age. Out of 58 patients, 45 (77.6%) were males and 13 (22.4%) were females. Mean duration of injury was 7.67 ± 2.28 weeks with majority of patients i.e. 62% were with less than 8 weeks of duration. Most common cause of persistent corneal epithelial defect was bacterial keratitis (17.2%) followed by vegetative trauma (13.8%). Successful surgical outcome as healed cornea was observed in 53 (91.4%) patients. Conclusion:  Amniotic membrane transplant is an effective treatment for persistent corneal epithelial defects unresponsive to standard medical treatment. This surgical technique is effective in terms of healing and resolving the inflammation and preserving the useful vision. Key Words:  Persistant corneal epithelial defect, Amniotic Membrane, Bullous keratopathy.

Detection of Corneal Epithelial Defect Through Amniotic Membrane Patch by Fluorescein

Cornea ◽  
2005 ◽  
Vol 24 (3) ◽  
pp. 359-360 ◽  
Author(s):  
Akira Kobayashi ◽  
Shigeyuki Ijiri ◽  
Kazuhisa Sugiyama ◽  
Mario A Di Pascuale ◽  
Scheffer C. G Tseng
Keyword(s):  
Amniotic Membrane ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Membrane Patch

scholarly journals Efficacy of rhNGF-loaded amniotic membrane transplantation for rabbit corneal epithelial and nerve regeneration

2021 ◽  
Vol 14 (11) ◽  
pp. 1653-1659
Author(s):  
Lu-Qin Wan ◽  
◽  
Hui-Feng Wang ◽  
Chen Chen ◽  
Hua Li ◽  
...  
Keyword(s):  
Sustained Release ◽  
Nerve Regeneration ◽  
Nerve Fiber ◽  
Amniotic Membrane ◽  
Control Group ◽  
Epithelial Defect ◽  
Corneal Sensitivity ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect

AIM: To evaluate the efficacy of recombinant human nerve growth factor-loaded amniotic membrane (rhNGF-AM) on corneal epithelial and nerve regeneration in rabbit model. METHODS: Freshly prepared human amniotic membrane (AM) were immersed into PBS buffer containing 100 or 500 μg/mL rhNGF for 15, 30, and 60min at 4℃. The in vitro release kinetics of rhNGF was measured with ELISA. For in vivo evaluation, the AM were immersed with 500 μg/mL rhNGF for 30min. Fifty-seven rabbits were selected to establish corneal epithelial defect model. In addition to the 19 rabbits in control group, 38 rabbits received AM transplantation with or without rhNGF after the removal of central epithelium. Corneal epithelial defect area, sub-epithelial nerve fiber density, corneal sensitivity, rhNGF contents in resident AM and corneas were measured after the surgery. RESULTS: rhNGF was sustained release from the AM within 14d in vitro, with the positive correlation with initial immersion concentration. The immersion of AM in 500 μg/mL rhNGF for 30min achieved the most stable release within 14d. After transplantation in rabbit cornea, a high concentration of rhNGF in resident rhNGF-AM and cornea was maintained within 8d. Corneal epithelial healing, nerve fiber regeneration and the recovery of corneal sensitivity were significantly accelerated after the rhNGF-AM transplantation when compared to simple AM transplantation (all P<0.05). CONCLUSION: Simple immersion of AM achieves the sustained release of rhNGF, and promotes corneal epithelial wound healing and nerve regeneration, as well as the recovery of corneal sensitivity in rabbit.

scholarly journals Tissue-Cultured Human Cord Lining Epithelial Cells in Treatment of Persistent Corneal Epithelial Defect

2019 ◽  
Vol 7 (24) ◽  
pp. 4266-4271 ◽  
Author(s):  
Nguyen Dinh Ngan ◽  
Hoang Minh Chau ◽  
Pham Ngoc Dong ◽  
Le Xuan Cung ◽  
Nguyen Thu Thuy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Early Stage ◽  
Case Series ◽  
Influential Factors ◽  
Lamellar Keratoplasty ◽  
Corneal Epithelial Cell ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Transplant Site

BACKGROUND: Persistent corneal epithelial defect (PED) is a consequence of many ocular surface disorders. Although many therapies have been suggested, the treatment of this disease have faced a lot of difficulties up to now. The transplatation of cultivated amniotic epithelial cells sheets is the new promised method for PED. Cord lining epithelial cells (CLECs) are epithelial cells of amniotic membrane of umbilical cord, so these cultivated cells sheet may be good for treating PED AIM: To evaluate the efficacy of the transplantation of cultivated CLECs sheets in treatment of PED and analyze some influential factors of this therapy. METHODS: A prospective interventional case series with transplantation of tissue-cultured human CLECs in 37 PED eyes in Vietnam National Institute of Ophthalmology. RESULTS: Thirty four of 37 eyes were healed with the cells transplantation and 22 eyes of them healed within a week postoperatively. There were normal corneal scars and normal corneal epithelial cell (by impression cytology detection) on transplantation site in all 31 successful cases. The other successful eyes were done lamellar keratoplasty (respectively in 1 month, 3 months, 6 months and 27 months postoperatively) to investigate the histopathology of the CLECs transplant site. The histopathological images showed normal corneal scar and there was no appearance of CLECs in transplant site. CONCLUSION: tissue-cultured human CLECs transplantation is a quite safe and effective treatment for persistent corneal epithelial defect. The CLECs may help the epithelial healing at early stage but do not exist at transplant site for a long time.

Severe Corneal Complications Associated With Corneal Lubricant Used During Surgery

2017 ◽  
Vol 1 (3) ◽  
pp. 187-190
Author(s):  
Andrew C. Lee ◽  
E. Mitchel Opremcak ◽  
Christiane Hunt ◽  
Juan A. Astruc ◽  
Brian Toussaint ◽  
...  
Keyword(s):  
Treatment Method ◽  
The United States ◽  
Posterior Segment ◽  
Epithelial Defect ◽  
Chemical Burns ◽  
Corneal Epithelial ◽  
Corneal Injury ◽  
Corneal Epithelial Defect ◽  
Time Period ◽  
Litmus Paper

Purpose: To present a series of cases of corneal chemical burns associated with the use of likely improperly buffered corneal lubricant gel, Goniovisc, during uncomplicated, posterior segment surgery. Methods: Cases of postoperative corneal complications following surgeries in which Goniovisc was used were collected from 6 retina practices across the United States between January and August 2016. The preoperative diagnosis, type of procedure, postoperative day 1 corneal findings, treatment method, and time to resolution of the corneal irregularity were recorded. pH testing of Goniovisc lots from this time period was performed using Hydrion litmus paper. Results: A total of 157 eyes of 156 patients were found to have corneal complications following relatively simple posterior segment surgeries in which Goniovisc was used. Findings included one or more of the following: corneal epithelial defect (84% of cases), Descemet folds (34%), stromal edema (14%), and superficial punctate keratitis (3%). Time to resolution ranged from 2 to 90 days (mean, 12 days; median, 7 days). Treatment methods included artificial tears (37%), bandage contact lens (28%), antibiotic drops (17%), antibiotic ointment (16%), and patching (9%). Two cases were treated with amniotic membrane grafts. One case required a penetrating keratoplasty due to an old laser-assisted in situ keratomileusis (LASIK) flap with a nonhealing epithelial defect that scarred significantly. Litmus testing of Goniovisc lots from this time period revealed a pH of 4 to 5. Conclusion: Chemical burns secondary to improperly buffered corneal lubricant solution should be considered in the differential when corneal injury is encountered following posterior segment surgery.

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