scholarly journals The war against cancer: Suicide gene therapy

2016 ◽  
Vol 2 (3) ◽  
pp. 139
Author(s):  
Muzeyyen Izmirli ◽  
Dilara Sonmez ◽  
Bulent Gogebakan
Keyword(s):  
Gene Therapy ◽  
Cytochrome P450 ◽  
Side Effects ◽  
Cancer Cells ◽  
Thymidine Kinase ◽  
Viral Vectors ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Kinase Gene ◽  
Cancer Society

<p>The National Cancer Institute and the American Cancer Society announced that 1.6 million new cancer cases are projected to occur in the USA in 2016. One of the most innovative approaches against cancer is suicide gene therapy, in which suicide-inducing transgenes are introduced into cancer cells. When cancer treatments target the total elimination of tumor cells, there will be no side effects for normal cells. Cancer tissues are targeted through various targeted transport methods, followed by tissue-specific enzymes converting a systemically suitable prodrug into an active drug in the tumor. Suicidal genes are delivered by transporters, such as viral and non-viral vectors, into cancer cells. Suicide gene therapeutic strategies currently pursued are herpes simplex virus thymidine kinase gene with prodrug ganciclovir, cytosine deaminase gene, carboxyl esterase/irinotecan, varicella zoster virus thymidine kinase/6-methoxypurine arabinonucleoside, nitroreductase Nfsb/5-(aziridin-1-yl)-2,4-dinitrobenzamide, carboxypeptidase G2/4-[(2-chloroethyl)(2- mesyloxyethyl)amino]benzoyl-L-glutamic acid, cytochrome p450-isofosfamide, and cytochrome p450-cyclophosphamide. The goal of this review is to summarize the different suicide gene systems and gene delivery vectors addressed to cancer cells, with a particular emphasis on recently developed systems. Finally, we briefly describe the advantageous clinical applications and potential side effects of suicide gene therapy. </p>

Are hepatomas a good target for suicide gene therapy? An experimental study in rats using retroviral-mediated transfer of thymidine kinase gene

Surgery ◽  
1998 ◽  
Vol 123 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Héléna Nagy ◽  
Yves Panis ◽  
Monique Fabre ◽  
Hubert Perrin ◽  
David Klatzmann ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Experimental Study ◽  
Thymidine Kinase ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Good Target

Suicide gene therapy for prostate cancer using a replication-deficient adenovirus containing the herpesvirus thymidine kinase gene

2000 ◽  
Vol 18 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Moshe Shalev ◽  
Brian J. Miles ◽  
Timothy C. Thompson ◽  
Gustavo Ayala ◽  
Edward B. Butler ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Thymidine Kinase ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Thymidine Kinase Gene ◽  
Kinase Gene

Graphene oxide–hydroxyapatite nanocomposites effectively deliver HSV-TK suicide gene to inhibit human breast cancer growth

2018 ◽  
Vol 33 (2) ◽  
pp. 216-226 ◽  
Author(s):  
Tuck-Yun Cheang ◽  
Yi-Yan Lei ◽  
Zhan-Qiang Zhang ◽  
Hong-Yan Zhou ◽  
Run-Yi Ye ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Graphene Oxide ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Transfection Efficiency ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Human Breast ◽  
Kinase Gene ◽  
Ganciclovir Treatment

Gene therapy with herpes simplex virus thymidine kinase gene (HSV-TK), which is also known as “suicide” gene therapy, is effective in various tumor models. The lack of a safe and efficient gene delivery system has become a major obstacle to “suicide” gene therapy. In this study, the cytotoxicity and transfection efficiency of graphene oxide–hydroxyapatite (GO–Hap) were analyzed by MTS and flow cytometry, respectively. A series of assays were performed to evaluate the effects of GO–HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment on growth of human breast normal and cancer cells. The results showed that GO–HAp nanocomposites effectively transfected cells with minimum toxicity. GO–HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment inhibited the proliferation and induced cell apoptosis in cancer cells, while the cytotoxic effects are tolerable in normal breast cells. We conclude that the GO–HAp nanocomposites have significant potential as a gene delivery vector for cancer therapy.

Carbon nanotube-delivered iC9 suicide gene therapy for killing breast cancer cells in vitro

Nanomedicine ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. 1033-1047 ◽  
Author(s):  
Masoud Mohseni-Dargah ◽  
Shiva Akbari-Birgani ◽  
Zahra Madadi ◽  
Fatemeh Saghatchi ◽  
Babak Kaboudin
Keyword(s):  
Breast Cancer ◽  
Gene Therapy ◽  
Carbon Nanotube ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Suicide Gene ◽  
Suicide Gene Therapy

Secretion of thymidine kinase to increase the effectivity of suicide gene therapy results in the loss of enzymatic activity

2008 ◽  
Vol 16 (1) ◽  
pp. 26-35
Author(s):  
A. M. J. Beerens ◽  
M. G. Rots ◽  
B. Bermúdez ◽  
E. F. J. de Vries ◽  
H. J. Haisma
Keyword(s):  
Gene Therapy ◽  
Enzymatic Activity ◽  
Thymidine Kinase ◽  
Suicide Gene ◽  
Suicide Gene Therapy

Herpes Simplex Virus Thymidine Kinase-Mediated Suicide Gene Therapy Using Nano/Microbubbles and Ultrasound

2008 ◽  
Vol 34 (3) ◽  
pp. 425-434 ◽  
Author(s):  
Atsuko Aoi ◽  
Yukiko Watanabe ◽  
Shiro Mori ◽  
Masahiko Takahashi ◽  
Georges Vassaux ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Simplex Virus
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