scholarly journals Survival of a Phosphate Solubilizing Microorganism in Ion-Sterile Carriers

2010 ◽  
Vol 12 (3,4) ◽  
pp. 219
Author(s):  
F. Er ◽  
M. Ogut
Keyword(s):  
Population Density ◽  
Liquid Culture ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Organic Fertilizer ◽  
Population Densities ◽  
Cold Tolerant ◽  
Number Of Microorganisms ◽  
Phosphate Solubilizing

<p>A cold-tolerant phosphate solubilizing bacterium (PSB) was isolated from roots of ryegrass (<em>Lolium perenne</em> L.). Studies involving phosphate solubilization in liquid culture and survival of the PSB in non-sterile zeolite, leonardite, peat, rock phosphate, and an organic fertilizer were performed. The PSB was able to dissolve 163 ppm P with a simultaneous fall in pH (from 7.7 to 5.7) in Pikovskaya’s medium during a ten-day incubation. The number of PSB declined logarithmically in 28 <sup>o</sup>C incubation regardless of the carrier. The rate of decrease in PSB population was less pronounced in zeolite. However, the PSB’s population density increased up to 10<sup>9</sup> cfu g<sup>-1</sup>, and stayed in the range of 10<sup>8</sup> to 10<sup>9</sup> cfu g<sup>-1</sup> in zeolite and rock phosphate after 13-weeks of storage at +4 <sup>o</sup>C. The contaminant microorganisms also grew in the carriers, with population densities ranging between 10<sup>8</sup> to 10<sup>9</sup> cfu g<sup>-1</sup> at week-9. The suppression of the local microorganisms is required to increase the quality of organic fertilizer by the addition of PSB. Zeolite could be a good carrier, due to its large surface area and porosity, which allow high number of microorganisms to occupy.</p>

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

Optimization for rock phosphate solubilization by phosphate-solubilizing fungi isolated from phosphate mines

2008 ◽  
Vol 33 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Chun-Qiao Xiao ◽  
Ru-An Chi ◽  
Xiao-Hui Huang ◽  
Wen-Xue Zhang ◽  
Guan-Zhou Qiu ◽  
...  
Keyword(s):  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Rock Phosphate Solubilization ◽  
Phosphate Mines ◽  
Phosphate Solubilizing

scholarly journals Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P

2016 ◽  
Vol 8 (2) ◽  
pp. 62
Author(s):  
Toto Hadiarto ◽  
Ma'sumah Ma'sumah ◽  
Eny I. Riyanti
Keyword(s):  
Phosphate Solubilization ◽  
Chemical Fertilizer ◽  
Fertilizer N ◽  
Genetic Quality ◽  
Phosphate Solubilizing

<p>Azospirillum sp. which has the ability for nitrogen<br />fixation and phosphate solubilization may support modern<br />farming in Indonesia that is mostly dependent on the usage<br />of chemical fertilizer N, P, and K. Genetic quality of<br />Azospirillum was improved in this research to obtain<br />superior characters toward phosphate solubilization so that<br />it can become more effective in use for farmers. To achieve<br />this goal, Azospirillum was mutated by means of<br />electroporation using transposon EZ-Tn5&lt;kan-2&gt;Tnp. The<br />electrotransformation resulted in 20 out of 22 transformants<br />tested contained the marker gen (npt). 10, 6 and 4 mutants<br />have increased, decreased and lost phosphate-solubilizing<br />function, respectively. Mutant with elevated phosphatesolubilizing<br />ability may be selected further to be utilized as<br />biofertilizer while others may be useful for identification of<br />genes responsible for phosphate solubilization.</p>

Rock phosphate-enriched organic fertilizer with phosphate-solubilizing microorganisms improves nodulation, growth, and yield of legumes

2018 ◽  
Vol 49 (21) ◽  
pp. 2715-2725 ◽  
Author(s):  
Allah Ditta ◽  
Muhammad Imtiaz ◽  
Sajid Mehmood ◽  
Muhammad Shahid Rizwan ◽  
Fathia Mubeen ◽  
...  
Keyword(s):  
Rock Phosphate ◽  
Organic Fertilizer ◽  
Growth And Yield ◽  
Phosphate Solubilizing

scholarly journals Vermicomposting: An Effective Option for Recycling Organic Wastes

2020 ◽  
Author(s):  
Tamanreet Kaur
Keyword(s):  
Low Cost ◽  
Organic Fertilizer ◽  
Organic Wastes ◽  
Growth Promoter ◽  
Phosphate Solubilizing Bacteria ◽  
Suitable Alternative ◽  
Effective Option ◽  
Phosphate Solubilizing ◽  
Water And Soil Pollution

Urbanization and industrialization resulted in rapid increase in volume of solid waste; its management has become one of the biggest problems today. Solid wastes can be disposed off by methods like land filling, incineration, conversion into biogas, recycling, and composting, but its overproduction has led to inappropriate disposal practices such as their indiscriminate and inappropriately timed application to agricultural fields that ultimately leads to water and soil pollution. However, if handled properly, these organic wastes can be used for vermicomposting; it is an effective recycling technology that improves the quality of the products which is disinfected, detoxified, and highly nutritive. It is a low cost, eco-biotechnological process of waste management in which earthworms are used to cooperate with microorganisms in order to convert biodegradable wastes into organic fertilizer. Earthworms excreta (vermicast) is a nutritive organic fertilizer rich in humus, NPK, micronutrients, beneficial soil microbes; nitrogen-fixing, phosphate solubilizing bacteria, actinomycets, and growth hormones auxins, gibberlins and cytokinins, is a suitable alternative to chemical fertilizers, being an excellent growth promoter and protector for crop plants. Thus, vermiculture not only results in management of soild waste but also produces excellent nutrient enriched vermicompost. Vermicompost is beneficial for sustainable organic agriculture and maintaining balanced ecosystem.

scholarly journals The Effect of Rock Phosphate and Level of Inoculums on The Survivability of Aspergillus niger and Its Solubilization Ability When Pelleted With Rock Phosphate

2005 ◽  
Vol 7 (2) ◽  
pp. 77-80
Author(s):  
Yudi Sastro ◽  
Donny Widianto ◽  
Irfan D Prijambada
Keyword(s):  
Organic Matter ◽  
Aspergillus Niger ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Soluble Phosphate ◽  
Christmas Island ◽  
Randomized Design ◽  
Soluble Phosphorus ◽  
Phosphate Solubilizing ◽  
Solubilization Ability

The study is intended to examine the survival of Aspergillus niger and its phosphate solubilizing ability when pelletedwith rock phosphate. An A. niger YD 17 obtainedfrom the Laboratory of Microbiology. Faculty of Agriculture, Gadjah MadaUniversity was used. The pellet was made by mixing rock phosphate (80%) with organic matter (J 3. 9% waste of tapioca. 6% rice bran, and 1% starch) and spore of A. niger. The experimental design was the Complete Randomized Design 5x4 with 3 replicatiOns. The first factor was sources of rock phosphate (Christmas Island. Jordan, China, Ciamis, and Madura). The secondfactor was the number ofinoculums i.e. control without inoculums, 107 • J(t and J(t cfu.g·'. The colony of A. niger that formed at medium of potatoes dextrose agar (PDA) and the amount of soluble phosphorus in the Pikovskaya liquid medium were parameters. Experimental results indicated that sources of rock phosphate and the number of inoculums itif1uence the A. niger survivability and its phosphorus solubilizing ability. Rock phosphate from Ciamis gave the best support for fungus survival and rock phosphate from Christmas Island was the best substrate for phosphate solubilization. The highest soluble phosphate was achieved by ul inoculums.

scholarly journals Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

Revista CERES ◽  
2014 ◽  
Vol 61 (3) ◽  
pp. 414-423
Author(s):  
Lílian Estrela Borges Baldotto ◽  
Marihus Altoé Baldotto ◽  
Fábio Lopes Olivares ◽  
Adriane Nunes de Souza
Keyword(s):  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Diazotrophic Bacteria ◽  
Rock Phosphates ◽  
Combined Application ◽  
Phosphate Solubilizing ◽  
Biological Nitrogen

Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i) to select and characterize diazotrophs able to solubilize phosphates in vitro and (ii) evaluate the initial performance of the pineapple cultivars Imperial and Pérola in response to inoculation with selected bacteria in combination with rock phosphate. The experiments were conducted at Universidade Estadual do Norte Fluminense Darcy Ribeiro, in 2009. In the treatments with bacteria the leaf contents of N, P and K were higher than those of the controls, followed by an increase in plant growth. These results indicate that the combined application of diazotrophic phosphate-solubilizing bacteria Burkholderia together with Araxá rock phosphate can be used to improve the initial performance of pineapple slips.

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