scholarly journals Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P

2016 ◽  
Vol 8 (2) ◽  
pp. 62
Author(s):  
Toto Hadiarto ◽  
Ma'sumah Ma'sumah ◽  
Eny I. Riyanti
Keyword(s):  
Phosphate Solubilization ◽  
Chemical Fertilizer ◽  
Fertilizer N ◽  
Genetic Quality ◽  
Phosphate Solubilizing

<p>Azospirillum sp. which has the ability for nitrogen<br />fixation and phosphate solubilization may support modern<br />farming in Indonesia that is mostly dependent on the usage<br />of chemical fertilizer N, P, and K. Genetic quality of<br />Azospirillum was improved in this research to obtain<br />superior characters toward phosphate solubilization so that<br />it can become more effective in use for farmers. To achieve<br />this goal, Azospirillum was mutated by means of<br />electroporation using transposon EZ-Tn5&lt;kan-2&gt;Tnp. The<br />electrotransformation resulted in 20 out of 22 transformants<br />tested contained the marker gen (npt). 10, 6 and 4 mutants<br />have increased, decreased and lost phosphate-solubilizing<br />function, respectively. Mutant with elevated phosphatesolubilizing<br />ability may be selected further to be utilized as<br />biofertilizer while others may be useful for identification of<br />genes responsible for phosphate solubilization.</p>

scholarly journals Survival of a Phosphate Solubilizing Microorganism in Ion-Sterile Carriers

2010 ◽  
Vol 12 (3,4) ◽  
pp. 219
Author(s):  
F. Er ◽  
M. Ogut
Keyword(s):  
Population Density ◽  
Liquid Culture ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Organic Fertilizer ◽  
Population Densities ◽  
Cold Tolerant ◽  
Number Of Microorganisms ◽  
Phosphate Solubilizing

<p>A cold-tolerant phosphate solubilizing bacterium (PSB) was isolated from roots of ryegrass (<em>Lolium perenne</em> L.). Studies involving phosphate solubilization in liquid culture and survival of the PSB in non-sterile zeolite, leonardite, peat, rock phosphate, and an organic fertilizer were performed. The PSB was able to dissolve 163 ppm P with a simultaneous fall in pH (from 7.7 to 5.7) in Pikovskaya’s medium during a ten-day incubation. The number of PSB declined logarithmically in 28 <sup>o</sup>C incubation regardless of the carrier. The rate of decrease in PSB population was less pronounced in zeolite. However, the PSB’s population density increased up to 10<sup>9</sup> cfu g<sup>-1</sup>, and stayed in the range of 10<sup>8</sup> to 10<sup>9</sup> cfu g<sup>-1</sup> in zeolite and rock phosphate after 13-weeks of storage at +4 <sup>o</sup>C. The contaminant microorganisms also grew in the carriers, with population densities ranging between 10<sup>8</sup> to 10<sup>9</sup> cfu g<sup>-1</sup> at week-9. The suppression of the local microorganisms is required to increase the quality of organic fertilizer by the addition of PSB. Zeolite could be a good carrier, due to its large surface area and porosity, which allow high number of microorganisms to occupy.</p>

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

Freshwater pearl culture in China.

2020 ◽  
Author(s):  
Lin Cao
Keyword(s):  
Shell Length ◽  
Culture Period ◽  
Production Cycle ◽  
High Quality ◽  
Host Fish ◽  
Genetic Quality ◽  
Operative Care ◽  
Artificial Breeding

Abstract Artificial breeding of freshwater pearl mussels is widely used to improve the yield of pearl culture. All phases of the production cycle, including collection and culture of the broodstock, release of the glochidia, provision of the host fish for glochidia to attach to, and collection of mussel seeds, can be controlled artificially. The advantages of artificial breeding are that it can help to produce high quality pearls and improve the genetic quality of pearl mussels. Collected mussel seed are transferred from holding jars into small 200 µm mesh baskets (10 cm diameter x 5 cm). Each basket was supplied individually with 0.1-0.2 L of water per minute. When the mussels' shell length reaches over 10 cm, they can be operated to culture pearl. After post-operative care the implanted mussels are stocked in ponds. The mussels are kept in nylon bags (2 mussels per bag) and are hung from bamboo or PVC pipes and placed in ponds at 1 m depth. Periodical checking of mussels, with removal of dead ones and cleaning of bags, is required throughout the culture period of 12-18 months.

scholarly journals Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 383 ◽  
Author(s):  
Gustavo Enrique Mendoza-Arroyo ◽  
Manuel Jesús Chan-Bacab ◽  
Ruth Noemi Aguila-Ramírez ◽  
Benjamín Otto Ortega-Morales ◽  
René Efraín Canché Solís ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Inorganic Phosphate ◽  
Extracellular Polymeric Substances ◽  
Phosphate Solubilization ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Habanero Pepper ◽  
Phosphate Solubilizing

The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

scholarly journals Complete Genome Sequence of Burkholderia cenocepacia CR318, a Phosphate-Solubilizing Bacterium Isolated from Corn Root

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Filip Zekic ◽  
Brian Weselowski ◽  
Ze-Chun Yuan
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Phosphate Solubilization ◽  
Burkholderia Cenocepacia ◽  
Ecological Sustainability ◽  
Content Type ◽  
Phosphate Solubilizing ◽  
Circular Chromosomes ◽  
Insight Into

ABSTRACT Here, we report the complete genome sequence of the phosphate-solubilizing bacterium Burkholderia cenocepacia CR318, consisting of three circular chromosomes of 3,511,146 bp, 3,097,552 bp, and 1,056,069 bp. The data presented will facilitate further insight into the mechanisms of phosphate solubilization and its application for agricultural and ecological sustainability.

PHOSPHATE SOLUBILIZATION BY FUNGI ASSOCIATED WITH LEGUME ROOT NODULES

1967 ◽  
Vol 13 (7) ◽  
pp. 749-753 ◽  
Author(s):  
P. K. Chhonkar ◽  
N. S. Subba-Rao
Keyword(s):  
Tricalcium Phosphate ◽  
Phosphate Solubilization ◽  
Root Nodules ◽  
Potassium Dihydrogen Phosphate ◽  
Dihydrogen Phosphate ◽  
Potassium Dihydrogen ◽  
Aspergillus Sp ◽  
Phosphate Solubilizing

Phosphate solubilizing ability of different isolates of fungi associated with legume root nodules was studied in vitro. Among the fungi tested, isolates of Penicillium lilacinum, Aspergillus sp., A. flavus, A. niger, A. terreus, and A. nidulans solubilized insoluble tricalcium phosphate. When soluble potassium dihydrogen phosphate was present with tricalcium phosphate in the medium, some of the fungi failed to solubilize phosphate.

scholarly journals Effect of Formulated Fertilization on Fruit Quality of Early Ripe Peach

2019 ◽  
Vol 136 ◽  
pp. 07016
Author(s):  
Changbing Pu ◽  
Qiao Xiao ◽  
Jin Wang ◽  
Dong Liang ◽  
Xiulan Lv
Keyword(s):  
Fruit Quality ◽  
Orthogonal Experiment ◽  
Organic Fertilizer ◽  
Chemical Fertilizer ◽  
Test Material ◽  
Peach Fruit ◽  
Early Maturing ◽  
Set Up ◽  
Trace Element Fertilizers

Using the early maturing peach variety 'Japan Matsusen' as the test material, nine groups of formula fertilization schemes were set up by orthogonal experiment to study the effects of different fertilization treatments on the quality of peach fruit, in order to obtain the best fertilization formula to improve fruit quality. The results showed that the quality of peach fruit was significantly improved by adding organic fertilizer to the base fertilizer instead of chemical fertilizer. Therefore, by adding organic fertilizer as the base fertilizer, reducing the application amount of chemical fertilizer, and adding other trace element fertilizers can effectively improve the quality of peach fruit.

Genetic improvement programs for aquaculture species

2003 ◽  
Vol 2003 ◽  
pp. 225-225
Author(s):  
B. Gjerde ◽  
B. Villanueva
Keyword(s):  
Genetic Improvement ◽  
Human Population ◽  
Feeding Practices ◽  
Food Crops ◽  
Genetic Quality ◽  
Genetically Improved ◽  
Improvement Programs ◽  
High Yields ◽  
Aquaculture Production

The high yields obtained in agriculture rely heavily on the use of domesticated and genetically improved breeds and varieties. Until quite recently this has not been the case for most farmed aquaculture species that, in the genetic sense, are still much closer to the wild state than are the major terrestrial animals and food crops. Less than 10 % of the total world aquaculture production is based on improved strains. Due to a growing human population and a decline in production from capture fisheries, there is therefore a great disparity between the need for increased aquaculture production and the genetic quality of the strains available to meet that need. Moreover, full benefits of investments in management improvements (feed and feeding practices, control of diseases, etc.) can only be obtained through the use of genetically improved animals.

Sign in / Sign up

Export Citation Format

Share Document