scholarly journals Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus.

2006 ◽  
Vol 53 (2) ◽  
pp. 371-376 ◽  
Author(s):  
Beata Gromadzka ◽  
Bogusław Szewczyk ◽  
Grazyna Konopa ◽  
Andrzej Fitzner ◽  
Andrzej Kesy
Keyword(s):  
Disease Virus ◽  
Rna Virus ◽  
Full Length ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Vp60 Gene ◽  
A Genome ◽  
Potential Vaccine ◽  
Domestic Rabbits

Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.

scholarly journals Comparative Phylodynamics of Rabbit Hemorrhagic Disease Virus in Australia and New Zealand

2015 ◽  
Vol 89 (18) ◽  
pp. 9548-9558 ◽  
Author(s):  
John-Sebastian Eden ◽  
John Kovaliski ◽  
Janine A. Duckworth ◽  
Grace Swain ◽  
Jackie E. Mahar ◽  
...  
Keyword(s):  
New Zealand ◽  
Disease Virus ◽  
South Australia ◽  
Evolutionary Rates ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Evolutionary Analysis ◽  
Rabbit Hemorrhagic Disease ◽  
A Genome ◽  
Viral Emergence

ABSTRACTThe introduction of rabbit hemorrhagic disease virus (RHDV) into Australia and New Zealand during the 1990s as a means of controlling feral rabbits is an important case study in viral emergence. Both epidemics are exceptional in that the founder viruses share an origin and the timing of their release is known, providing a unique opportunity to compare the evolution of a single virus in distinct naive populations. We examined the evolution and spread of RHDV in Australia and New Zealand through a genome-wide evolutionary analysis, including data from 28 newly sequenced RHDV field isolates. Following the release of the Australian inoculum strain into New Zealand, no subsequent mixing of the populations occurred, with viruses from both countries forming distinct groups. Strikingly, the rate of evolution in the capsid gene was higher in the Australian viruses than in those from New Zealand, most likely due to the presence of transient deleterious mutations in the former. However, estimates of both substitution rates and population dynamics were strongly sample dependent, such that small changes in sample composition had an important impact on evolutionary parameters. Phylogeographic analysis revealed a clear spatial structure in the Australian RHDV strains, with a major division between those viruses from western and eastern states. Importantly, RHDV sequences from the state where the virus was first released, South Australia, had the greatest diversity and were diffuse throughout both geographic lineages, such that this region was likely a source population for the subsequent spread of the virus across the country.IMPORTANCEMost studies of viral emergence lack detailed knowledge about which strains were founders for the outbreak or when these events occurred. Hence, the human-mediated introduction of rabbit hemorrhagic disease virus (RHDV) into Australia and New Zealand from known starting stocks provides a unique opportunity to understand viral evolution and emergence. Within Australia, we revealed a major phylogenetic division between viruses sampled from the east and west of the country, with both regions likely seeded by viruses from South Australia. Despite their common origins, marked differences in evolutionary rates were observed between the Australian and New Zealand RHDV, which led to conflicting conclusions about population growth rates. An analysis of mutational patterns suggested that evolutionary rates have been elevated in the Australian viruses, at least in part due to the presence of low-fitness (deleterious) variants that have yet to be selectively purged.

The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants

Plant Science ◽  
2002 ◽  
Vol 162 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Sonia Castañón ◽  
José M. Martı́n-Alonso ◽  
Marı́a S. Marı́n ◽  
José A. Boga ◽  
Pablo Alonso ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Potato Plants ◽  
Vp60 Gene

scholarly journals Rabbit Hemorrhagic Disease Virus Isolated from Diseased Alpine Musk Deer (Moschus sifanicus)

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 897
Author(s):  
Shijun Bao ◽  
Kai An ◽  
Chunguo Liu ◽  
Xiaoyong Xing ◽  
Xiaoping Fu ◽  
...  
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Phylogenetic Analyses ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Musk Deer ◽  
Rabbit Hemorrhagic Disease ◽  
Vp60 Gene

Rabbit hemorrhagic disease virus (RHDV) is the causative agent of rabbit hemorrhagic disease (RHD), and its infection results in mortality of 70–90% in farmed and wild rabbits. RHDV is thought to replicate strictly in rabbits. However, there are also reports showing that gene segments from the RHDV genome or antibodies against RHDV have been detected in other animals. Here, we report the detection and isolation of a RHDV from diseased Alpine musk deer (Moschussifanicus). The clinical manifestations in those deer were sudden death without clinical signs and hemorrhage in the internal organs. To identify the potential causative agents of the disease, we used sequence independent single primer amplification (SISPA) to detect gene segments from viruses in the tissue samples collected from the dead deer. From the obtained sequences, we identified some gene fragments showing very high nucleotide sequence similarity with RHDV genome. Furthermore, we identified caliciviral particles using an electron microscope in the samples. The new virus was designated as RHDV GS/YZ. We then designed primers based on the genome sequence of an RHDV strain CD/China to amplify and sequence the whole genome of the virus. The genome of the virus was determined to be 7437 nucleotides in length, sharing the highest genome sequence identity of 98.7% with a Chinese rabbit strain HB. The virus was assigned to the G2 genotype of RHDVs according to the phylogenetic analyses based on both the full-length genome and VP60 gene sequences. Animal experiments showed that GS/YZ infection in rabbits resulted in the macroscopic and microscopic lesions similar to that caused by the other RHDVs. This is the first report of RHDV isolated from Alpine musk deer, and our findings extended the epidemiology and host range of RHDV.

scholarly journals Recovery of Infectious Rabbit Hemorrhagic Disease Virus from Rabbits after Direct Inoculation with In Vitro-Transcribed RNA

2006 ◽  
Vol 80 (13) ◽  
pp. 6597-6602 ◽  
Author(s):  
Guangqing Liu ◽  
Yuying Zhang ◽  
Zheng Ni ◽  
Tao Yun ◽  
Zutian Sheng ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Clone ◽  
Full Length ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Progeny Virus ◽  
Cdna Clones ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Rna Transcripts

ABSTRACT We report the first full-length infectious clone of strain JX/CHA/97 of rabbit hemorrhagic disease virus (RHDV). The transcripts from the full-length cDNA clones were infectious when they were directly injected into rabbits. The sequence of the virus recovered from the rabbits was identical to that of the injected RNA transcripts. The cDNA clone was engineered to contain one silent nucleotide change to create an EcoRV site (A to T at nucleotide 2908). The genetic marker was retained in the recovered progeny virus. The transfection of RNA transcripts into RK-13 cells resulted in the synthesis of viral antigens, indicating that the cDNA clones were replication competent. This stable infectious molecular clone should be an important tool for developing a better understanding of the molecular biology and pathogenesis of RHDV.

Sign in / Sign up

Export Citation Format

Share Document