Anti-inflammatory Effects of Matricaria chamomilla Extracts on BALB/c Mice Macrophages and Lymphocytes

Matricaria chamomilla (MC) was shown to have anti-inflammatory effects. Flavonoids are major groups of MC immunomodulators. The anti-inflammatory effects of apigenin as an MC flavonoid has already been demonstrated. In this study, we aimed to report the amount of this compound by liquid chromatography-mass spectrometry (LC-MS) and measuring the total phenol content (TPC) in both the MC aqueous and alcoholic extracts. We also investigated the MC aqueous and ethanolic extracts effect on BALB/c separated macrophages and lymphocytes cell viability and macrophage nitric oxide production. Interferon-γ and interleukin-10 secretion were also measured in lymphocytes. We found that the amount of apigenin was 0.078 and 0.25 mg/g per each of dry aqueous and alcoholic extracts, respectively. Also, the total phenol content was 2.99% in aqueous and 3.95% in alcoholic extracts. BALB/c separated macrophages cell viability significantly increased when treated with the MC aqueous extract but decreased when treated by the MC alcoholic extract in the presence of lipopolysaccharide. Also, the amount of nitric oxide production by macrophages and BALB/c separated lymphocytes cell viability in treatment with aqueous and alcoholic extracts significantly decreased. Interferon-γ increased, and interleukin-10 decreased in lymphocytes treated with the MC aqueous extract, which may suggest Th1 polarization. There was no significant change in the interferon-γ level in lymphocytes when treated with the MC alcoholic extract, but the level of IL-10 increased in these cells. Altogether, besides the anti-inflammatory effect of MC extracts, we found MC aqueous extract effects as disrupting Th1/Th2 balance to Th1 upregulation. Overall, the anti-inflammatory effect of the MC alcoholic extract was higher than the MC aqueous extract.

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Detection About The Anti-Inflammatory Effect in Alcoholic Extract of RosmarinusofficinalisIn vitro

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2018.10.04.078 ◽

2018 ◽

Vol 10 (4) ◽

Keyword(s):

Alcoholic Extract ◽

Anti Inflammatory ◽

Inflammatory Effect

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Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽

10.3390/molecules26113303 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3303

Author(s):

Amina M. G. Zedan ◽

Mohamed I. Sakran ◽

Omar Bahattab ◽

Yousef M. Hawsawi ◽

Osama Al-Amer ◽

...

Keyword(s):

Phenolic Compounds ◽

Aqueous Extract ◽

Antioxidant Activities ◽

Individual Treatment ◽

Alcoholic Extract ◽

Anticancer Effect ◽

Mcf7 Cells ◽

Vespa Orientalis ◽

Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

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Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/735176 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Ewelina Szliszka ◽

Anna Mertas ◽

Zenon P. Czuba ◽

Wojciech Król

Keyword(s):

Cell Viability ◽

Radical Scavenging ◽

Gm Csf ◽

Raw264.7 Macrophages ◽

Griess Reaction ◽

Artepillin C ◽

Anti Inflammatory ◽

Radical Scavenging Ability ◽

Brazilian Green Propolis ◽

Inflammatory Effect

Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis. The aim of this study was to investigate the anti-inflammatory effect of artepillin C on LPS + IFN-γ- or PMA-stimulated RAW264.7 macrophages. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH•and ABTS•+. ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated RAW264.7 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. NF-κB activity was confirmed by the ELISA-based TransAM NF-κB kit. At the tested concentrations, the compound did not decrease the cell viability and did not cause the cytotoxicity. Artepillin C exerted strong antioxidant activity, significantly inhibited the production of ROS, RNS, NO, and cytokine IL-1β, IL-3, IL-4, IL-5, IL-9, IL-12p40, IL-13, IL-17, TNF-α, G-CSF, GM-CSF, MCP-1, MIP-1α, MIP-1β, RANTES, and KC, and markedly blocked NF-κB expression in stimulated RAW264.7 macrophages. Our findings provide new insights for understanding the mechanism involved in the anti-inflammatory effect of artepillin C and support the application of Brazilian green propolis in complementary and alternative medicine.

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A comparative study of the anti-inflammatory effect of aqueous extract of neem leaf and dexamethasone

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v3i1.836 ◽

2008 ◽

Vol 3 (1) ◽

Cited By ~ 4

Author(s):

Abu Syed Md. Mosaddek ◽

Md. Mamun Ur Rashid

Keyword(s):

Comparative Study ◽

Aqueous Extract ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-Inflammatory Effects of Antidepressants Through Suppression of the Interferon-γ/Interleukin-10 Production Ratio

Journal of Clinical Psychopharmacology ◽

10.1097/00004714-200104000-00012 ◽

2001 ◽

Vol 21 (2) ◽

pp. 199-206 ◽

Cited By ~ 217

Author(s):

Marta Kubera ◽

Ai-Hua Lin ◽

Gunter Kenis ◽

Eugene Bosmans ◽

Dirk van Bockstaele ◽

...

Keyword(s):

Interleukin 10 ◽

Interferon Γ ◽

Production Ratio ◽

Anti Inflammatory

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Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/neq036 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 29

Author(s):

Morgana Duarte da Silva ◽

Giselle Guginski ◽

Maria Fernanda de Paula Werner ◽

Cristiane Hatsuko Baggio ◽

Rodrigo Marcon ◽

...

Keyword(s):

Vascular Permeability ◽

Interleukin 10 ◽

Sham Acupuncture ◽

Therapeutic Effects ◽

Plasma Extravasation ◽

Sterile Saline ◽

Cell Counts ◽

Cytokine Levels ◽

Anti Inflammatory ◽

Inflammatory Effect

In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 μg/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.

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