Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 μg/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.

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Acupuncture reduces peripheral and brainstem cytokines in rats subjected to lipopolysaccharide-induced inflammation

Acupuncture in Medicine ◽

10.1177/0964528420938379 ◽

2020 ◽

pp. 096452842093837

Author(s):

Caroline C Ramires ◽

Daniela TL Balbinot ◽

Francisco J Cidral-Filho ◽

Daniel Ventura Dias ◽

Adair RS dos Santos ◽

...

Keyword(s):

Vascular Permeability ◽

Intraperitoneal Injection ◽

Peritoneal Fluid ◽

Inflammatory Responses ◽

Acupuncture Point ◽

Sterile Saline ◽

Cell Counts ◽

Cytokine Levels ◽

Anti Inflammatory ◽

Inflammatory Effect

Background: Lipopolysaccharide (LPS) endotoxins are activators of innate immunity inducing infection and inflammatory responses. Anti-inflammatory drugs can have undesirable side effects. Acupuncture may be an alternative for the treatment of inflammatory processes. Objective: We investigated the potential anti-inflammatory effect of manual acupuncture (MA) at SP6 upon LPS-induced peritonitis in rats. Methods: Peritonitis was induced in rats with an intraperitoneal injection of LPS (0.002, 0.02, 0.2 or 2 µg/kg) in four experimental groups (n = 6 each). A fifth group was injected with sterile saline solution (saline group, n = 6). Four hours after the procedure, peritoneal fluid was collected to determine total cell counts for inflammatory cells, differential leukocyte counts and peritoneal capillary permeability. The LPS dose of 0.02 µg/kg was used in the subsequent experiments as it most successfully induced peritoneal inflammation. Subsequently, five experimental groups (n = 12 rats each) were used: (1) saline, (2) control (untreated LPS group), (3) indomethacin (LPS group treated with indomethacin), (4) NA (LPS group treated with MA at a location not corresponding to any traditional acupuncture point), and (5) SP6 (LPS group treated with verum MA at SP6). Ten minutes after MA or 30 min after indomethacin treatment, the rats received an intraperitoneal injection of LPS. After 4 h, total leukocyte and differential cell counts, myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated in the peritoneal fluid. Cytokine levels were additionally evaluated in the brainstem. Results: SP6 MA and indomethacin treatments reduced inflammatory cell infiltration, vascular permeability and MPO activity in the LPS-exposed rats. Pre-treatment with indomethacin and SP6 MA decreased tumor necrosis factor (TNF)-α levels and preserved interleukin (IL)-10 in the peritoneal fluid. Indomethacin also reduced IL-6 in the peritoneal fluid. In the brainstem, indomethacin reduced IL-1β, IL-6, TNFα and IL-10, whereas SP6 MA reduced only TNFα and IL-6 levels. Conclusions: This study clearly demonstrates the anti-inflammatory effect of acupuncture, which we believe may involve the activation of anti-inflammatory neural reflexes in the regulation of peritonitis.

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Tobacco Exposure by Various Modes May Alter Proinflammatory (IL-12) and Anti-Inflammatory (IL-10) Levels and Affects the Survival of Prostate Carcinoma Patients: An Explorative Study in North Indian Population

BioMed Research International ◽

10.1155/2014/158530 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 14

Author(s):

Shailendra Dwivedi ◽

Apul Goel ◽

Sanjay Khattri ◽

Anil Mandhani ◽

Praveen Sharma ◽

...

Keyword(s):

Cancer Patients ◽

Prostate Carcinoma ◽

Interleukin 10 ◽

Interleukin 12 ◽

Tobacco Exposure ◽

Statistical Tool ◽

Cytokine Levels ◽

Test Graph ◽

Anti Inflammatory ◽

Alcohol Users

Objective. Inflammation is an important hallmark of all cancers and net inflammatory response is determined by a delicate balance between pro- and anti-inflammatory cytokines, which may be affected by tobacco exposure, so the present study was designed to explore the effect of various modes of tobacco exposure on interleukin-12 (IL-12) and interleukin-10 (IL-10) inflammatory cytokine levels and survival in prostate carcinoma (PCa) patients.Methods. 285 cancer patients and equal controls with 94 BPH (benign prostatic hyperplasia) were recruited; baseline levels of serum IL-12 and IL-10 were measured and analyzed in various tobacco exposed groups by appropriate statistical tool. Five-year survivals of patients were analyzed by Log-rank (Mantel-Cox) test (graph pad version 5).Results. The expression of serum proinflammatory (IL-12) and anti-inflammatory (IL-10) cytokines was correlated with tobacco exposed group as smokers, chewers, and alcohol users have shown significantly higher levels (P<0.001) with significantly lower median survivals (27.1 months, standard error = 2.86, and 95% CI: 21.4–32.62); than nonusers. Stages III and IV of tobacco addicted patients have also shown significantly increased levels of IL-12 and IL-10.Conclusions. IL-12 and IL-10 seem to be affected by various modes of tobacco exposure and inflammation also affects median survival of cancer patients.

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The Effect of Chronic Spice Consumption on Plasma Pro-Inflammatory Cytokine Levels and Monocyte Function in Adults with Overweight/Obesity

Current Developments in Nutrition ◽

10.1093/cdn/nzaa068_015 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1530-1530

Author(s):

Ester Oh ◽

Kristina Petersen ◽

Penny Kris-Etherton ◽

Connie Rogers

Keyword(s):

Inflammatory Cytokine ◽

Mononuclear Cells ◽

Transendothelial Migration ◽

Test Period ◽

Low Grade ◽

Monocyte Subset ◽

Cytokine Levels ◽

Anti Inflammatory ◽

Classical Monocytes ◽

Inflammatory Effect

Abstract Objectives Obesity-induced, chronic, low-grade inflammation is involved in the pathogenesis of cardiovascular disease (CVD). Numerous spices have anti-inflammatory properties in animal models and humans. However, few studies have examined the anti-inflammatory effect of spices in the context of daily meal consumption, which is typically how spices are consumed. The objective of this study was to investigate the anti-inflammatory effect of chronic spice consumption in adults with overweight/obesity at risk for CVD. Methods Nonsmoking adults (30–75 years old) with overweight/obesity (BMI ≥ 25 and ≤ 35 kg/m2), elevated waist circumference (≥94 cm for men and ≥ 80 cm for women) and at least one other risk factor for CVD were recruited for a 3-period, crossover, randomized controlled-feeding study (n = 63). In random order, participants consumed an Average American Diet (AAD) for 4 weeks containing: 1) 0.6 g of spice blend per 2100 kcal, 2) 3.2 g of spice blend per 2100 kcal, or 3) 6.4 g of spice blend per 2100 kcal with a≥2-week washout period between each test period. The spice blend was comprised of 24 popular spices. Blood was collected at baseline and after each test period. Peripheral blood mononuclear cells were isolated, and the % of monocyte subsets (classical; CD14++CD16−, intermediate; CD14++CD16+, non-classical; CD14+CD16++) were quantified using flow cytometry. Plasma pro-inflammatory cytokine levels (TNF-α, IL-1β, IL-6, IL-8, MCP-1) were measured using ELISA. In a subset of participants (n = 6), transendothelial migratory function of each monocyte subset through MCP-1 stimulated human umbilical vein endothelial cells was evaluated. Results Plasma IL-6 was significantly reduced after consuming the AAD containing 3.2 g compared to 0.6 g of spice blend in men and postmenopausal women (P = 0.031). Transendothelial migration of classical monocytes was significantly reduced following consumption of the AAD containing 3.2 g and 6.4 g of the spice blend compared to 0.6 g of spice blend (P = 0.011). Conclusions Consumption of an AAD with spices for 4 weeks attenuated inflammatory outcomes including plasma IL-6 and transendothelial migration of classical monocytes in adults with overweight/obesity. Funding Sources McCormick Science Institute.

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Time course of pro- and anti-inflammatory cytokine levels in patients with burns—Prognostic value of interleukin-10

Burns ◽

10.1016/j.burns.2009.10.009 ◽

2010 ◽

Vol 36 (4) ◽

pp. 483-494 ◽

Cited By ~ 30

Author(s):

C. Csontos ◽

V. Foldi ◽

L. Pálinkas ◽

L. Bogar ◽

E. Röth ◽

...

Keyword(s):

Prognostic Value ◽

Inflammatory Cytokine ◽

Time Course ◽

Interleukin 10 ◽

Cytokine Levels ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine

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Anti-Inflammatory Effect of Crocus sativus on Serum Cytokine Levels in Subjects with Metabolic Syndrome: A Randomized, Double-Blind, Placebo- Controlled Trial

Current Clinical Pharmacology ◽

10.2174/1574884712666170622082737 ◽

2018 ◽

Vol 12 (2) ◽

pp. 122-126 ◽

Cited By ~ 10

Author(s):

Tayebe Kermani ◽

Maedeh Zebarjadi ◽

Hassan Mehrad-Majd ◽

Seyed-Reza Mirhafez ◽

Maryam Shemshian ◽

...

Keyword(s):

Metabolic Syndrome ◽

Controlled Trial ◽

Crocus Sativus ◽

Serum Cytokine ◽

Double Blind ◽

Double Blind Placebo ◽

Cytokine Levels ◽

Anti Inflammatory ◽

Inflammatory Effect

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Therapeutic Effects of Sodium Hyaluronate and Diclofenac Sodium in Chronic Arthritis

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v7i4.536 ◽

2019 ◽

Vol 7 (4) ◽

pp. 17-33

Author(s):

Alsadek H Bogzil ◽

Gamal Shams ◽

Sohair Abd El-Latif

Keyword(s):

Experimental Model ◽

Diclofenac Sodium ◽

Sodium Hyaluronate ◽

Chronic Arthritis ◽

Albino Rats ◽

Therapeutic Effects ◽

Monosodium Iodoacetate ◽

Anti Inflammatory ◽

Inflammatory Effect

The present study was designed to compare the anti-inflammatory effect of sodium hyaluronate, which is similar to the lubricant fluid that found naturally in the capsule of the healthy joint with diclofenac sodium, a member of NSAIDs commonly used in treatment of Osteoarthritis (OA), separately and in combination on an experimental model of osteoarthritis in rats induced by monosodium-iodoacetate (MIA). Twenty-five male albino rats weighing at the beginning of the experiment 160± 20 gm were used in this study. Rats were housed in cages at 25± 0.5°C. The rats were divided into 5 main groups.

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The Effects of Moxibustion on PD-1/PD-L1-Related Molecular Expression and Inflammatory Cytokine Levels in RA Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6658946 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Yumei Zhong ◽

Deli Lai ◽

Linlin Zhang ◽

Wenting Lu ◽

Yanan Shang ◽

...

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Inflammatory Reaction ◽

Moxibustion Therapy ◽

Adenovirus Vector ◽

Complementary Therapy ◽

Cytokine Levels ◽

Chronic Inflammatory Reaction ◽

Anti Inflammatory ◽

Inflammatory Effect

Objective. Rheumatoid arthritis (RA) is an autoimmune disease that starts with inflammation of the synovium. The pain and joint dysfunction caused by RA urgently need an effective treatment to alleviate the inflammatory reaction and delay the progression of the disease. The pathological damage of RA is proposed to associate with the dysfunction of the programmed cell death 1/programmed cell death ligand 1 (PD-1/PD-L1) pathway. Moxibustion, as a main complementary therapy of traditional Chinese medicine (TCM), has been proved effective to reduce chronic inflammatory reaction on RA, but whether the anti-inflammatory effects are mediated by PD-1/PD-L1 pathway is still unclear. Therefore, moxibustion was conducted in the rats with RA to investigate its effect on PD-1/PD-L1. Methods. The rats' right hind paws were injected with Freundʼs complete adjuvant (FCA) to establish the model of RA. Seven days after the injection of FCA, moxibustion therapy was performed on the acupoints of Shenshu (BL23) and Zusanli (ST36) once a day for three weeks. Then, ELISA and immunohistochemical methods were used to analyze the influence of moxibustion on the expression of PD-1/PD-L1. If the moxibustion had an effect on the expression of PD-1/PD-L1-related molecules, we would knock down PD-1 with adenovirus vector. After moxibustion therapy, ELISA and histological analysis were performed to observe the anti-inflammatory effect of moxibustion. Results. The results demonstrated that moxibustion had an effect on the expression of PD-1-related molecules. The results of ELISA manifested that moxibustion decreased the level of IFN-γ and increased the level of IL-4 and IL-10. HE staining revealed that moxibustion alleviated the proliferation of synovial tissue. However, the anti-inflammatory effect and pathological improvement were weakened when PD-1 was blocked. Conclusions. The results indicate that moxibustion affected the expression of PD-1/PD-L1-related molecules and can effectively treat RA damage. The anti-inflammatory effect of moxibustion was weakened when PD-1 was knocked down.

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Comparison of the Therapeutic Effects of Gold Nanoclusters and Gold Nanoparticles on Rheumatoid Arthritis

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2019.2848 ◽

2019 ◽

Vol 15 (11) ◽

pp. 2281-2290 ◽

Cited By ~ 3

Author(s):

Yao Zhao ◽

Zhesheng He ◽

Ruoping Wang ◽

Pengju Cai ◽

Xiangchun Zhang ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Au Nanoparticles ◽

Type Ii Collagen ◽

Therapeutic Effects ◽

Type Ii ◽

Au Clusters ◽

Anti Inflammatory ◽

Inflammatory Effect

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and progressive cartilage and bone damage. In our previous studies, we found that Au clusters using glutathione as a template (GACs) produced profound anti-inflammatory effects in vitro on lipopolysaccharide (LPS)-induced inflammation in mouse macrophage RAW 264.7 cells and type II collagen-induced rat RA in vivo. In this study, we examined whether the template for Au clusters synthesis has an effect on its anti-inflammatory effect and whether Au nanoparticles with larger particle diameter produce the same anti-inflammatory effect. We synthesized Au clusters with bovine serum albumin (BSA) as a template (BACs), Au clusters with glutathione (GSH) as a template (GACs), and Au nanoparticles with glutathione as a template (GANs) and compared their anti-inflammatory effects in vitro and in vivo. These three Au nanomaterials can inhibit the production of lipopolysaccharide (LPS)-induced proinflammatory mediators and ameliorate type II collagen-induced rat RA. However, although the three Au nanomaterials produced similar anti-inflammatory effects, the GANs with larger particle sizes were less stable in vivo and accumulated in the peritoneum after intraperitoneal injection, resulting in poor absorption in vivo. The BACs showed relatively high liver accumulation due to the larger molecular weight of the outer shell. Therefore, we believe that the GACs are potential reliable nanodrugs for the treatment of RA.

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