Upregulation of miRNA-155 in Nasopharyngeal Carcinoma Pa-tients

Background: The upregulation of miRNA-155 (miR-155) has been associated with oncogenesis of many human tumors, including nasopharyngeal carcinoma (NPC). However, the profile of miR-155 in Vietnamese NPC patients has not been investigated. The current study aimed to evaluate the miR-155 expression and assess whether miR-155 is a potential biomarker for diagnosis of NPC in Vietnamese patients. Methods: In current case-control study, total of RNA was isolated from 60 biopsy NPC samples and 60 non-cancerous swab samples were analyzed by Reverse-transcription PCR, qualitative Real-time PCR. Results: The frequency of miR-155 detection were 78.33%, 15.0% in NPC and non-cancerous samples (P<0.05), respectively. The miR-155 expression level was 4.92 times higher in tumor samples than non-cancerous sample. Conclusion: Taken together, miR-155 in NPC was upregulated. It may serve as a potential biomarker for NPC in the Vietnamese population.

Download Full-text

Association Between LMP-1, LMP-2, and miR-155 Expression as Potential Biomarker in Nasopharyngeal Carcinoma Patients: A Case/Control Study in Vietnam

Genetic Testing and Molecular Biomarkers ◽

10.1089/gtmb.2019.0089 ◽

2019 ◽

Vol 23 (11) ◽

pp. 815-822

Author(s):

Thuan Duc Lao ◽

Thuy Ai Huyen Le

Keyword(s):

Nasopharyngeal Carcinoma ◽

Case Control Study ◽

Case Control ◽

Potential Biomarker ◽

Control Study

Download Full-text

Postirradiated carotid blowout syndrome in patients with nasopharyngeal carcinoma: A case-control study

Head & Neck ◽

10.1002/hed.23671 ◽

2014 ◽

Vol 37 (6) ◽

pp. 794-799 ◽

Cited By ~ 16

Author(s):

Kun-Chih Chen ◽

Ting-Ting Yen ◽

Yi-Ling Hsieh ◽

Hung-Chieh Chen ◽

Rong-San Jiang ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Case Control Study ◽

Case Control ◽

Carotid Blowout Syndrome ◽

Control Study

Download Full-text

The association of male infertility with telomere length: A case control study

Indian Journal of Clinical Anatomy and Physiology ◽

10.18231/j.ijcap.2021.070 ◽

2021 ◽

Vol 8 (4) ◽

pp. 325-332

Author(s):

Kate Deepali Rajesh ◽

Puranam Vatsalaswamy ◽

Manvikar Purshotam Rao

Keyword(s):

Male Infertility ◽

Telomere Length ◽

Real Time ◽

Real Time Pcr ◽

Case Control Study ◽

Case Control ◽

Control Group ◽

Significant Difference ◽

Control Study ◽

Sperm Telomere Length

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

Download Full-text

Estimation of Salivary 8-Hydroxydeoxyguanosine (8-OHdG) as a Potential Biomarker in Assessing Progression towards Malignancy: A Case-Control Study

Asian Pacific Journal of Cancer Prevention ◽

10.31557/apjcp.2020.21.8.2325 ◽

2020 ◽

Vol 21 (8) ◽

pp. 2325-2329

Author(s):

Arulmozhi Nandakumar ◽

Priyadharsini Nataraj ◽

Amritha James ◽

Rajkumar Krishnan ◽

Mahesh K M

Keyword(s):

Case Control Study ◽

Case Control ◽

Potential Biomarker ◽

Control Study

Download Full-text

Association of a Novel KIF26B Gene Polymorphism with Susceptibility to Schizophrenia and Breast Cancer: A Case-Control Study

Iranian Journal of Public Health ◽

10.18502/ijph.v50i2.5359 ◽

2021 ◽

Author(s):

Saman SARGAZI ◽

Milad HEIDARI NIA ◽

Shekoufeh MIRINEJAD ◽

Mahdiyeh MOUDI ◽

Mahdiyeh JAFARI SHAHROUDI ◽

...

Keyword(s):

Breast Cancer ◽

Case Control Study ◽

De Novo ◽

Case Control ◽

Amplification Refractory Mutation System ◽

Salting Out ◽

Current Case ◽

Increased Risk ◽

Mutation System ◽

Control Study

Background: KIF26B gene is found to play essential roles in regulating different aspects of cell proliferation and development of the nervous system. We aimed to determine if rs12407427 T/C polymorphism could affect susceptibility to schizophrenia (SZN) and breast cancer (BC), the two genetically correlated diseases. Methods: The current case-control study was performed from Aug 2018 to Dec 2018. Briefly, 159 female pathologically confirmed BC cases referring to Alzahra Hospital, Isfahan, Iran, and 102 psychologically confirmed SZN patients (60 males and 42 females) admitted to Baharan Hospital, Zahedan, Iran, were enrolled. Using the salting-out method, genomic DNA was extracted, and variants were genotyped using allele-specific amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method. Results: The results revealed a significant association between the KIF26B rs12407427 codominant CT (P=0.001), CC (P=0.0001), dominant CT+CC, and recessive CC (P=0.001) genotypes with the risk of developing SZN. Significant correlations were also found regarding rs12407427 and BC susceptibility in different inheritance models, including over-dominant CT (P=0.026), dominant CT+CC (P=0.001), recessive CC (P=0.009), and codominant CT and CC (P=0.001) genotypes. The over-presence of the C allele was also correlated with an increased risk for SZN (P=0.0001) and BC (P=0.0001). Finally, computational analysis predicted that T/C variation in this polymorphism could change the binding sites in proteins involved in splicing. Conclusion: rs12407427 T/C as a de novo KIF26B variant might be a novel genetic biomarker for SZN and/or BC susceptibility in a sample of the Iranian population.

Download Full-text

Predictive Markers for Malignant Urothelial Transformation in Balkan Endemic Nephropathy: A Case–Control Study

Cancers ◽

10.3390/cancers12102945 ◽

2020 ◽

Vol 12 (10) ◽

pp. 2945

Author(s):

Gordana Kocic ◽

Jovan Hadzi-Djokic ◽

Jovana Cukuranovic-Kokoris ◽

Mihajlo Gajic ◽

Andrej Veljkovic ◽

...

Keyword(s):

Case Control Study ◽

P53 Protein ◽

Case Control ◽

Predictive Markers ◽

Balkan Endemic Nephropathy ◽

Current Case ◽

Soluble Fas ◽

Igf I ◽

Endemic Nephropathy ◽

Control Study

Balkan endemic nephropathy (BEN) is a chronic tubulointerstitial disease frequently accompanied by urothelial carcinoma (UC). In light of the increased UC incidence and the markers observed in BEN patients with developed UC, the aim of the current case–control study is to assess survivin, p53 protein, growth factors and receptors (VEGF, VEGFR1, IGF I, IGF-1R and IGFBP5), tumor marker (TF)/CD142, circulating soluble Fas receptor and neopterin, as potentially predictive markers for UC in patients with BEN (52 patients), compared to healthy, age-matched subjects (40). A threefold increase was registered in both circulating and urinary survivin level in BEN patients. Especially noticeable was the ratio of U survivin/U Cr level five times the ratio of BEN patients associated with standard renal markers in multivariate regression models. The concentrations of VEGF, VEGFR1, (TF)/CD142, (sFas) were not significantly different in BEN patients, while urinary/plasma level demonstrated a significant decrease for VEGF. The levels of IGF I, IGFBP5 and IGF-1R were significantly reduced in the urine of BEN patients. Plasma concentration of neopterin was significantly higher, while urinary neopterin value was significantly lower in BEN patients compared to healthy controls, which reflected a significantly lower urine/plasma ratio and low local predictive value. As BEN is a slow-progressing chronic kidney disease, early detection of survivin may be proposed as potential predictor for malignant alteration and screening tool in BEN patients without the diagnosis of UC.

Download Full-text