scholarly journals The association of male infertility with telomere length: A case control study

2021 ◽  
Vol 8 (4) ◽  
pp. 325-332
Author(s):  
Kate Deepali Rajesh ◽  
Puranam Vatsalaswamy ◽  
Manvikar Purshotam Rao
Keyword(s):  
Male Infertility ◽  
Telomere Length ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Significant Difference ◽  
Control Study ◽  
Sperm Telomere Length

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

scholarly journals The Relationship between Serum Levels of Vitamin C, Uric Acid and Antioxidant Condition with Coronary Artery Diseases: a Case-Control Study

2019 ◽  
Author(s):  
Aida Torkzaban ◽  
Seyed Amir Mansour Alavi Naeini ◽  
Akbar Hassanzadeh ◽  
Mehrdad Namdari
Keyword(s):  
Uric Acid ◽  
Vitamin C ◽  
Case Control Study ◽  
Demographic Data ◽  
Systolic Pressure ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Significant Difference ◽  
Control Study

Abstract Background Coronary hearth diseases are among the main causes of death in adults. Increase of oxidative stress and defects in antioxidant defense play a major role in endothelium performance and are an effective factor in progress of atherosclerosis. Some studies have also reported different malondialdehyde and total antioxidant capacity among the atherosclerosis patients.Methods In this case-control study, 44 atherosclerosis patients referring to Shahid Madani treatment-education center were considered as the case group; while 44 healthy peoples were placed in the control group. Demographic data and anthropometric indices were measured. Food frequency questionnaire and international physical activity questionnaires were also completed. After 12 hours of fasting, 10 ml blood was sampled from the participants. Uric acid, vitamin C, TAC and MDA were also measured. The data were finally analyzed by SPSS Ver 22 software.Results A significant difference was observed between the two groups in terms of uric acid (P<0.001) and vitamin C (P<0.03). However, mean MDA and TAC showed no significant difference between the two groups. The two groups’ difference in terms of vitamin A, E and beta carotene, zinc and selenium intake was not significant. A significant difference was however detected between the two groups in terms of vitamin C (P<0.047). A significant relationship was also observed between the systolic pressure and CHD (P<0.028).Conclusion Results of this study indicated that the uric acid and vitamin C levels of atherosclerosis patients had significant increase and decrease in comparison with the healthy subjects, respectively. Mean TAC and antioxidant levels of their diets (except for vitamin C) showed no significant difference. Systolic blood pressure of the patients was significantly higher than the controls.

scholarly journals Interaction between serum FGF-23 and PTH in renal phosphate excretion, a case-control study in hypoparathyroid patients

2019 ◽  
Author(s):  
Forough Saki ◽  
Seyed Reza Kassaee ◽  
Azita Salehifar Salehifar ◽  
gholamhossein Ranjbar omrani
Keyword(s):  
Parathyroid Hormone ◽  
Serum Calcium ◽  
Case Control Study ◽  
Fractional Excretion ◽  
Case Control ◽  
Control Group ◽  
Phosphate Excretion ◽  
Significant Difference ◽  
Fgf 23 ◽  
Control Study

Abstract Background:phosphate homeostasis is mediated through complex counter regulatory feed-back balance between parathyroid hormone, FGF-23 and 1,25(OH)2D. Both parathyroid hormone and FGF-23 regulate proximal tubular phosphate excretion through signaling on sodium- phosphate cotransporters II a and II c . However, the interaction between these hormones on phosphate excretion is not clearly understood. We performed the present study to evaluate whether the existence of sufficient parathyroid hormone is necessary for full phosphaturic function of FGF-23 or not. Methods:In this case-control study, 19 patients with hypoparathyroidism and their age- and gender-matched normal population were enrolled. Serum calcium, phosphate, alkaline phosphatase,parathyroid hormone, FGF-23, 25(OH)D, 1,25(OH)2D and Fractional excretion of phosphorous were assessed and compared between the two groups, using SPSS software. Results:The mean serum calcium and parathyroid hormone level was significantly lower in hypoparathyroid patients in comparison with the control group(P<0.001 and P<0.001, respectively). We found high serum level of phosphate and FGF-23 in hypoparathyroid patients compared to the control group (P<0.001 and P<0.001,respectively). However, there was no significant difference in Fractional excretion of phosphorous or 1,25OH2D level between the two groups. There was a positive correlation between serum FGF-23 and Fractional excretion of phosphorous just in the normal individuals (P <0.001, r = 0.79). Conclusions:Although the FGF-23 is a main regulator of urinary phosphate excretion but the existence of sufficient parathyroid hormone is necessary for the full phosphaturic effect of FGF-23.

Is There a Relationship between Patellofemoral Alignment and Osgood–Schlatter Disease? A Case-Control Study

2018 ◽  
Vol 33 (01) ◽  
pp. 067-072 ◽  
Author(s):  
Fırat Seyfettinoğlu ◽  
Özkan Köse ◽  
Hasan Ulaş Oğur ◽  
Ümit Tuhanioğlu ◽  
Hakan Çiçek ◽  
...  
Keyword(s):  
Case Control Study ◽  
Case Control ◽  
Radiographic Examination ◽  
Control Group ◽  
Etiologic Factor ◽  
Full Extension ◽  
Significant Difference ◽  
Patellofemoral Alignment ◽  
The Difference ◽  
Control Study

AbstractThis study was aimed to investigate the role of anatomic variations in patellofemoral alignment in patients with or without Osgood-Schlatter disease (OSD) and to determine the potential anatomic risk factors that may play role in the etiology. This prospective observational case-control study was conducted on two groups of adolescent patients. Group 1 comprised patients who were diagnosed as having OSD and group 2 consisted of an equal number of age-matched patients who presented to the outpatient clinic with traumatic knee injury and underwent knee radiographic examination but without a diagnosis of OSD. Age, height, weight, body mass index (BMI), dominant side, and level of sporting activity were recorded. Quadriceps (Q) angles were measured using a long-arm goniometer with patients lying in the supine position with their knees in full extension and contracting the Q muscles. On anteroposterior, lateral knee, and tangential patella (Merchant and Laurin views) radiographs, the following measurements were performed: Insall–Salvati (IS), Caton–Deschamps (CD), and Blackburne–Peel (BP) indexes, congruence angles, lateral patellofemoral angles, sulcus angles, and patella type according to Grelsamer's morphology classification. Both groups were similar in respect of age (p = 0.160), sex (p = 0.311), height (p = 0.326), weight (p = 0.596), BMI (p = 0.153), and dominancy (p = 0.500). The rate of patients engaged in sports activities was significantly greater in the OSD group (p = 0.003). No significant difference was determined between the groups in respect to IS index, CD index, BP index, sulcus angle, lateral patellofemoral angle, and congruence angle (p–values: 0.358, 0.995, 0.912, 0.802, 1.000, and 0.907, respectively). The mean Q angle was measured as 15.6 ± 2.2 degrees in the OSD group and 14.3 ± 2.5 degrees in the control group (p = 0.014). Despite the difference being statistically significant, it was clinically insignificant because the difference was only 1.3 degrees. The principle etiologic factor seems to be increased physical activity rather than subtle variations is patellofemoral anatomy and alignment of extensor mechanism.

scholarly journals Peri-implantitis and extracellular matrix antibodies: A case–control study

2017 ◽  
Vol 11 (03) ◽  
pp. 340-344 ◽  
Author(s):  
Piero Papi ◽  
Stefano Di Carlo ◽  
Daniele Rosella ◽  
Francesca De Angelis ◽  
Mario Capogreco ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Case Control Study ◽  
Test Group ◽  
Case Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Fisher Exact Test ◽  
Type I ◽  
Significant Difference ◽  
Control Study

ABSTRACT Objective: The aim of this case–control study was to compare patients with a healthy peri-implant environment and patients affected by peri-implantitis, evaluating the occurrence of antibodies to extracellular matrix (ECM) molecules. The authors hypothesized the presence of ECM autoantibodies in serum of peri-implantitis patients. Materials and Methods: Patients were divided into two groups: one with dental implants with a diagnosis of peri-implantitis and one control group with implants classified as being “healthy.” Enzyme-linked immunosorbent assay was performed on patients' sera to detect human antibodies to type I, III, IV, and V collagens, laminin, and fibronectin. Fisher exact test was performed to evaluate statistical association, with a significant P < 0.05. Results: Forty-two patients were enrolled in this study, 27 females (64.28%) and 15 males (35.72%) with a mean age of 53 ± 29.69 years (age range 32–74). The presence of antibodies to CIII was recorded in 6/21 (28.57%) patients of test group, compared to just 2/21 (9.52%) for the control group, showing a statistically significant difference (P < 0.05). Other antibodies tested were found to be not statistically significant or absent. Conclusions: Within the limitations of this study, it can be concluded that further studies, with larger sample and different design, are necessary to address the research purpose, evaluating possible associations between anti-ECM antibodies and peri-implantitis.

scholarly journals The Role of Toxoplasma gondii Infection Among Multiple Sclerosis Patient Compared to Ordinary People in South of Iran: A Case-Control Study

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Mahsa Rahnama ◽  
Qasem Asgari ◽  
Peiman Petramfar ◽  
Davod Tasa ◽  
Vahid Hemati ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Toxoplasma Gondii ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Ordinary People ◽  
Serum Samples ◽  
Significant Difference ◽  
Control Study

Background: Toxoplasmosis is a common parasitic disease, which leads to serious disease symptoms in immunocompromised hosts and infants. Recent epidemiologic surveys showed that microbial agents may be associated with some cases of multiple sclerosis (MS). Objectives: This case-control study aimed to examine the role of Toxoplasma gondii in MS by evaluating sero-frequency of anti-T. gondii IgG (ATXAb) antibody between patients with MS and ordinary people in Shiraz Province, south of Iran during 2016 - 2018. Methods: Serum samples obtained from MS patients (n = 130), and a group of age and gender-matched controls (n = 130) with the same socioeconomic status with the patients’ group were collected to evaluate the prevalence of T. gondii IgG antibodies (ATXAb). Moreover, the presence of the ATXAb antibody of the patients and controls was determined by the ELISA test. SPSS 20 software was used to perform the statistical analysis (SPSS, inc., Chicago, USA). Results: Out of 130 (35.4%) cases with MS and 130 (13.8%) controls, 46 and 18 subjects were seropositive for ATXAb, respectively. Based on the chi-square test, a significant difference was observed in terms of the positivity rates of ATXAb between the MS patient group and the control group (P = 0.001). The mean ± standard deviation ATXAb levels in controls and MS patients were found to be 61 ± 34 and 114 ± 47 IU/mL, respectively. Also, there were statistically significant differences between the levels of these two groups (P = 0.001). Conclusions: Given the relationship between toxoplasmosis and MS, it is possible that the prevalence of MS decreases by increasing hygiene and preventing toxoplasmosis.

scholarly journals Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case–control study

2021 ◽  
Author(s):  
Mehdi Alizadeh ◽  
Mahboobeh Nasiri ◽  
Morteza Samadi ◽  
Nasrin Ghasemi ◽  
Ali Moradi
Keyword(s):  
Statistical Analysis ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Paraoxonase 1 ◽  
Control Group ◽  
Case Group ◽  
Significant Difference ◽  
Control Study

Background: Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL. Objective: To investigate the relationship between Q192R and L55M polymorphisms of PON1 as antioxidant enzyme and the risk of RPL. Materials and Methods: In this case–control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Results: Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39–86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic frequency in the RPL participants compared to the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for MM genotype in the case group compared with the control group showed a significant difference (OR = 3.59, CI = 0.97–13.30, p = 0.042), but none for the LM and LL genotypes. Conclusion: The findings showed a significant correlation between the Q192R polymorphisms and the L55M PON1 enzyme and RPL in this study population. Key words: Pregnancy, Abortion, PON1, Polymorphism, Recurrent pregnancy loss.

Quality of Life in Children with Migraine: A Case–Control Study

2020 ◽  
Author(s):  
Saeid Sadeghian ◽  
Aliakbar Momen ◽  
Gholamreza Jelodar ◽  
Shahram Nasiri ◽  
Azin Khalafinia ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Case Control Study ◽  
Negative Impact ◽  
Case Control ◽  
Control Group ◽  
P Value ◽  
Quality Of Life Inventory ◽  
Significant Difference ◽  
Control Study

AbstractWe conducted a case–control study to determine the effects of migraine on quality of life (QOL) in children. Participants, aged between 6 and 18 years, were assigned to migraine (n = 70) and control (n = 70) groups. We used a translated version of Pediatric Quality of Life Inventory 4.0 questionnaire. From the children's perspective, the overall QOL scores in all domains were lower in children with migraine. Children in the migraine group had significant lower scores in the social, emotional, and physical functioning (p-value < 0.05) than control group. Interestingly, a significant difference in the educational performance was not seen (p-value = 0.101). Relative to the parents' responses and children, the overall scores in all domains were lower in children with migraine. Statistically significant lower scores were found only in emotional and physical domains (p-value < 0.05). The results of this study indicate that migraine imposes a substantial negative impact on the different aspects of QOL in children.

scholarly journals The Changes of Serum Lipids in Idiopathic Epiretinal Macular Membranes

2020 ◽  
Author(s):  
Liguo Feng ◽  
Ying Chen ◽  
Yesheng Xu ◽  
Wenjia Xie ◽  
Yi Sun ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Obese People ◽  
Serum Triglycerides ◽  
Significant Difference ◽  
Control Study ◽  
Group Serum ◽  
Cataract Patients

Abstract Purpose: To describe the changes of serum lipids in patients with idiopathic epiretinal macular membranes (iERMs). Methods: A retrospective, observational case control study was performed January 2015 and December 2019 in our unit. A total of 57 subjects with idiopathic epiretinal macular membranes were collected in the study. Senile cataract patients with age matched during hospitalizations were randomly collected as control group. iERMs were diagnosed based on OCT images. Comprehensive ophthalmologic examinations and Systemic examinations were performed. The changes of serum lipids were analyzed. Results: The iERMs was common in female (71.9%). Compared to control group, Serum triglycerides were higher in iERMs group (1.54 ± 0.66 vs 1.21 ± 0.50, P < 0.01). There was significant difference in BMI with iERM (23.68 ± 2.86 vs 22.50 ± 2.71, P = 0.03). After subjects stratified according whether with hypertension diseases, there was still statistical difference in serum triglycerides (P < 0.05). Conclusions: Serum triglycerides and BMI are elevated in patients with iERMs. It suggests that obese people seem to be prone to iERMs. Hyperlipemia seems to play a role in the formation of iERMs.

scholarly journals Frequency of the rs 14035 polymorphism of RAN gen in recurrent pregnancy loss: A case-control study

2020 ◽  
Author(s):  
Zahrasadat Mortazavifar ◽  
Hamidreza Ashrafzadeh ◽  
Seyed Morteza Seifati ◽  
Nasrin Ghasemi
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Nuclear Pore ◽  
Normal Delivery ◽  
Significant Difference ◽  
Control Study

Background: Genetic factors could account for recurrent pregnancy loss (RPL). The RAN gene is a member of the ”large RAS family” and a small GTPase that is essential for the translocation of Ribonucleic acid (RNA) and proteins through the nuclear pore. Mutation in the RAN constitutive gene could stop DNA synthesis and alter the expression of genes in the uterus, likely playing a role in recurrent miscarriage. Objective: The aim was to investigate the frequency of RAN (rs 14035) polymorphism in women with RPL compared with women without abortion history. Materials and Methods: In this case-control study, 100 women with at least two consecutive miscarriages before the 20th wk of gestation and having spouses with karyotype and normal sperm parameters as the case group and 100 women with no history of abortion and having at least one successful pregnancy and normal delivery as the control group. The groups were age matched (20-40 yr). The rs 14035 polymorphism of RAN gene was investigated by Polymerase Chain Reaction- Restriction Fragment Length poly morphism technique and the frequency of which was compared between the two groups. Results: The frequency of TT, TC, and CC genotypes of RAN gene polymorphism in the case group were 9%, 40%, and 51%, respectively, and in the control group were 11%, 38%, and 51%, respectively. There was no significant difference in the genotypes between two groups (p = 0.882). Conclusion: According to our results, it seems that RAN polymorphism (rs 14035) is not associated with the risk of RPL in this study population. Key words: RAN gene, Repeated abortion, Polymorphism, PCR-RFLP.

scholarly journals Analysis of 14-bp HLA-G polymorphism in middle-eastern women with recurrent implantation failure

2020 ◽  
Vol 9 (12) ◽  
pp. 4820
Author(s):  
Rand Sulaiman ◽  
Thara Mohamad ◽  
Marwan Alhalabi
Keyword(s):  
Case Control Study ◽  
Middle Eastern ◽  
Human Leukocyte ◽  
Case Control ◽  
Control Group ◽  
Polymorphism Analysis ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Significant Difference ◽  
Control Study

Background: Implantation appears to be the major rate-limiting step in assisted reproductive techniques success. Recurrent implantation failure (RIF) is a common condition, being the failure to achieve a clinical pregnancy after transfer of four or more good-quality embryos in a minimum of two IVF cycles in a woman under the age of 40. Human leukocyte antigen G (HLA-G) is thought to play a key role in implantation by modulating cytokine secretion to enable the semi-allogeneic embryo to implant into the maternal endometrium. The aim of this study was to investigate the association between the 14-bp ins/del HLA-G polymorphism and rates of RIF. No similar studies were conducted nationally or regionally.Methods: The study was designed as a case-control study. A proportion of 30 patients with RIF were selected as the experimental group. The control group included 15 patients with at least one pregnancy following IVF procedure. Peripheral blood samples were collected from both groups. DNA was extracted and the polymorphism analysis was performed using (AMPLI - HLA-G polymorphism) kit, then PCR products were electrophoresed on 2% agarose gel. statistical analysis was performed using SPSS statistics software.Results: A total of 45 middle-eastern patients were recruited in this case-control study. High frequency of the polymorphism was found in both case (76.6%) and control (86.67%) groups, but with no significant difference between the two groups (p=0.429).Conclusions: There is no correlation between the 14-bp ins/del HLA-G polymorphism and RIF, thus it may be considered as a normal polymorphism and might not affect the level of HLA-G.

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