ANTIOBESITY OF FUCOXANTHIN FROM Sargassumechinocarpum BY INCREASING β-OXIDATION IN ADIPOCYTE

Fucoxanthin is a one of carotenoids that contained in brown seaweeds. This compound has been affected to lipid metabolism. The aim of this study was to evaluate the antiobesity of fucoxanthin of Sargassumechinocarpum on adipocyte by increasing of adiponectin and decreasing of tumor necrosis factor a expressions. Fucoxanthin was isolated from Sargassumechinocarpum and validated by infrared spectrophotometer. Adipocyte was obtained from pre-adipocyte cell from viscera tissue of wistar rats (Rattusnorvegicus) and cultured in fetal bovine serum and Dulbeccos modified eagle’s medium. Adipocyte was treated with fucoxanthin and quercetin as control. Expression of adiponectin and tumor necrosis factor a of adipocyte was verified by enzyme link immunosorbent assay (ELISA) and observed by ELISA reader. The result showed that infrared spectra of Sargassumechinocarpumextract was equal with infrared spectra of fucoxanthin. Adipocyte cells treated with fucoxanthin showed increasing of adiponectin and decreasing of tumor necrosis factor a expression. It indicated that fucoxanthin able to enhance β-oxidation in adipocyte cells. In conclusion, our findings indicate that fucoxanthin from Sargassumechinocarpum able to increase adiponectin and decrease tumor necrosis factor a expression in adipocyte and it is promising to develop anantiobesitynutraceutical.Keywords: adipocyte, adiponectin, fucoxanthin, Sargassumechinocarpum, tumor necrosis factor α