scholarly journals Test Various Estrus Detection Device Against Pregnancy Rates on Dairy Cows in Cooperative Tunas Setia Baru Kabupaten Pasuruan

2017 ◽  
Vol 3 (6) ◽  
pp. 712
Author(s):  
Silvia Rani Andriyanti ◽  
Mas’ud Hariadi ◽  
Roesno Darsono ◽  
Pudji Srianto
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Dairy Cow ◽  
Artificial Insemination ◽  
Research Method ◽  
Control Sample ◽  
Pregnancy Rates ◽  
Treatment Groups ◽  
Detection Tool ◽  
Estrus Detection

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53.3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66.7%. Without treatment or control, sample with an average of (1.67±0.48) resulted in a  73.3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0.00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices. Keyword: estrus detection, artificial insemination, dairy cow, pregnancy rate

scholarly journals UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN

2020 ◽  
Vol 7 (2) ◽  
pp. 156
Author(s):  
Silvia Rani Andriyanti ◽  
Mas’ud Hariadi ◽  
Roesno Darsono ◽  
Pudji Srianto ◽  
Wurlina Wurlina ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Research Method ◽  
Control Sample ◽  
Pregnancy Rates ◽  
Anova Test ◽  
Treatment Groups ◽  
Detection Tool ◽  
Estrus Detection ◽  
In Pregnancy

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.

22 PREGNANCY RATES IN DAIRY COWS TREATED WITH PROGESTERONE-RELEASING DEVICES AND DIFFERENT GONADOTROPIN-RELEASING HORMONE OR ESTRADIOL PLUS EQUINE CHORIONIC GONADOTROPIN FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS

2008 ◽  
Vol 20 (1) ◽  
pp. 91 ◽  
Author(s):  
G. Veneranda ◽  
L. Filippi ◽  
D. Racca ◽  
L. Cutaia ◽  
G. A. Bo
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Milk Yield ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Device Removal ◽  
Treatment Groups ◽  
Lactating Dairy Cows

We have previously shown that the addition of eCG improved pregnancy rates following fixed-time artificial insemination (FTAI) in lactating dairy cows treated with estradiol benzoate (EB) and progesterone releasing devices (Veneranda et al. 2006 Reprod. Fertil. Dev. 18, 118). An experiment was designed to compare pregnancy rates in lactating dairy cows treated with progesterone-releasing devices and EB plus eCG with different gonadotropin-releasing hormone (GnRH)-based protocols. Five-hundred lactating dairy cows between 30 and 51 days postpartum (when prostaglandin F (PGF) treatments were given in the Pre-Synch groups), with a milk yield of 29.5 � 7.0 kg per day (range 13.0 to 46.0 kg) and a body condition score (BCS) between 2.5 to 3.5 out of 5 were used. Cows were blocked by days postpartum and randomly assigned to five treatment groups. Cows in the Pre-Synch group received PGF 28 and 14 days prior to the insertion of a DIB device (1 g progesterone, Syntex SA, Buenos Aires, Argentina) and the administration of 50 µg of Lecirelin (GnRH, Ovusin, Syntex SA) IM (Day 0). On Day 7, DIB were removed and cows received PGF (0.5 mg cloprostenol, Ciclase, Syntex SA). On Day 9, cows received a second GnRH treatment and were FTAI 16 h later (60 h after device removal). Cows in the Modified Pre-Synch group were treated similarly except that they received a DIB for 7 days prior to the second PGF injection and no DIB at the time of the first GnRH. Cows in the P4-Synch group were treated as those in the Pre-synch group but did not receive the two doses of PGF 28 and 14 days earlier. Cows in the P4 + EB + eCG groups received a DIB for 8 days, 2 mg of EB at DIB insertion (Day 0), and PGF and 400 IU eCG (Novormon, Syntex SA) IM at DIB removal (Day 8); they were subdivided to receive 1 mg of EB on Day 9 (P4 + EB + eCG + EB group) or GnRH on Day 10 (P4 + EB + eCG + GnRH group). Cows in these latter two groups were also FTAI 60 h after device removal. Pregnancy was determined by rectal palpation 50 days after FTAI and data were analyzed by logistic regression. Pregnancy rates were not affected by BCS, days postpartum, or milk yield (P > 0.5). However, there was a significant group effect due to a higher (P < 0.05) pregnancy rate in the P4 + EB + eCG + EB (49%) and P4 + EB + eCG + GnRH (44%) treatment groups than in those in the Modified Pre-Synch group (30%). Pregnancy rate in the P4-Synch group (39%) was lower (P < 0.05) than those in the P4 + EB + eCG + EB group but not different from the others. Finally, pregnancy rate in Pre-Synch group (41%) was intermediate and not different from the other treatment groups. Results of these experiments suggest that treatments with progesterone-releasing devices, EB, and eCG result in pregnancy rates that are comparable to or better than those obtained in GnRH-based protocols in lactating dairy cows. Further studies are needed to investigate whether eCG could increase pregnancy rates in cows treated with GnRH and progesterone-releasing devices. The authors thank Syntex SA for providing the hormones used in the study.

Effects of presynchronization and postinsemination treatments on pregnancy rates to a timed breeding Ovsynch protocol in dairy cows and heifers

2010 ◽  
Vol 90 (1) ◽  
pp. 35-44 ◽  
Author(s):  
M B Gordon ◽  
N Dinn ◽  
R Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Dairy Cow ◽  
Artificial Insemination ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Lactating Cows ◽  
Lactating Dairy Cows ◽  
Timed Artificial Insemination ◽  
To Receive

This study examined two strategies to improve pregnancy rate (PR) following Ovsynch timed artificial insemination (TAI) for lactating dairy cows (n = 225) and nulliparous heifers (n = 87). Animals were assigned randomly to receive one of three treatments: Ovsynch protocol (GnRH 7 d before and 48 h after one PGF2α treatment), Presynch + Ovsynch (two treatments of PGF2α 14 d apart followed by Ovsynch 14 d later), or Ovsynch + Post-AI GnRH (GnRH 6 d after Ovsynch TAI) for first service breeding. Pregnancy rates among treatments were not different in lactating cows (42.5, 48.0, and 44.9%) or heifers (65.5, 58.6, and 58.6%) for Ovsynch, Presynch + Ovsynch, and Ovsynch + Post-AI, respectively. Cows treated with Ovsynch had lower PR when bred < 76 d in milk (DIM) compared with Presynch + Ovsynch or Ovsynch + Post-AI treatments. In addition, cows and heifers that received Post-AI GnRH had greater progesterone (P4) concentrations on day 21 and day 28 post-TAI than the Ovsynch group. Animals with higher P4 concentrations at initiation of Ovsynch had better PR than those with low P4 concentrations. Presynch animals had a greater proportion of animals with P4 values above 1 ng mL-1 at the initiation of Ovsynch than those animals in the Ovsynch group (74.5 vs. 59.4%). Heifers had lower PR if they were <14.6 mo of age (48.9 vs. 75%) or weighed <380 kg (47.4 vs. 70.8%). Although no significance differences in PR were observed between treatments in cows or heifers, DIM in cows and age and weight in heifers affected PR.Key words: Ovsynch, presynchronization, gonadotropin-releasing hormone, dairy heifer, dairy cow, timed artificial insemination

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

7 COMPARISON OF TIMED ARTIFICIAL INSEMINATION PROTOCOLS USING PROSTAGLANDIN F2α+ESTRADIOL OR PROSTAGLANDIN F2α+ESTRADIOL+GONADOTROPIN-RELEASING HORMONE IN DAIRY COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 115
Author(s):  
J. K. Jeong ◽  
H. G. Kang ◽  
I. H. Kim
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Gnrh Analogue ◽  
Chi Square ◽  
Array Transducer ◽  
Chi Square Test ◽  
Timed Artificial Insemination

This study compared pregnancy rates following 2 timed artificial insemination (TAI) protocols using PGF2α + oestradiol or PGF2α + oestradiol + GnRH in dairy cows. Four hundred fifty-five Holstein dairy cows, 2.2 ± 0.1 in parity, 148.3 ± 3.6 days in milk, with corpus luteum of greater than 20 mm diameter confirmed by ultrasonography (Tringa Linear with 5.0 MHz array transducer; Esaote Pie Medical, Maastricht, the Netherlands) were randomly divided into two treatments: an injection of 500 μg of cloprostenol, PGF2α analogue (Iliren cycle BP®, Intervet International GmbH, Unterschleissheim, Germany) and an injection of 2 mg of oestradiol benzoate (EB, SY Esrone, Samyang, Seoul, Korea) 36 h later (PGF2α + EB group, n = 257), or an injection of 500 μg of cloprostenol, an injection of 2 mg of EB 36 h later and an additional injection of 100 μg of gonadorelin, GnRH analogue (Godorel, Uni-Biotech Co., Ltd., Korea) 24 h later (PGF2α + EB + GnRH group, n = 198). All cows in the two groups received TAI 24 h after the EB injection. Pregnancy was determined at 40 to 50 days after TAI by using both ultrasonography and rectal palpation. Pregnancy rates between the PGF2α + EB and PGF2α + EB + GnRH groups were compared by the chi-square test using the SAS program (version 9.1: SAS Institute Inc., Cary, NC, USA). Pregnancy rate following TAI did not differ between the PGF2α + EB (32.7%) and PGF2α + EB + GnRH groups (34.9%; P > 0.05). In conclusion, addition of GnRH for TAI protocol using PGF2α + oestradiol did not improve pregnancy rate in dairy cows.

19 PREGNANCY RATES IN DAIRY COWS TREATED WITH INTRAVAGINAL PROGESTERONE DEVICES AND DIFFERENT FIXED-TIME AI PROTOCOLS

2006 ◽  
Vol 18 (2) ◽  
pp. 118 ◽  
Author(s):  
G. Veneranda ◽  
L. Filippi ◽  
D. Racca ◽  
G. Romero ◽  
E. Balla ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Milk Yield ◽  
Body Condition Score ◽  
Animal Health ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Treatment Groups ◽  
Lactating Dairy Cows ◽  
To Receive

Two experiments were designed to evaluate the effect of different treatments for the synchronization of ovulation on pregnancy rates following fixed-time AI (FTAI) of lactating dairy cows. In Experiment 1, 394 Holstein cows that were 61.7 � 13.6 days postpartum (range 35 to 94 days), with a milk yield of 30.7 � 6.8 liters per day (range 12.0 to 52.4 liters) and a body condition score (BCS) between 2.5 to 3.5 out of 5, were used. Cows were blocked by days postpartum and milk yield and randomly assigned to one of four treatment groups. Cows in P4+EB treatment groups received an intravaginal DIB device (1 g P4; Syntex, Argentina) and 2 mg of estradiol benzoate (EB; Syntex) i.m. on Day 0. On Day 8, DIB devices were removed, and cows received PGF (150 �g D+cloprostenol: Ciclase; Syntex), and were subdivided to receive 400 IU eCG (Novormon 5000, Syntex) i.m. or no further treatment at that time. On Day 9, all cows received 1 mg of EB and were FTAI 60 h after DIB removal. Cows in P4-Synch groups received a DIB device and 50 �g of GnRH (Lecirelina, Gonasyn; Syntex) i.m. on Day 0. On Day 7, DIB devices were removed; cows received PGF and were divided to receive 400 IU eCG i.m. or no further treatment. On Day 9, all cows received a second GnRH treatment and were FTAI 60 h after DIB removal. Blood samples were taken on Days -10 and 0 to determine plasma P4 concentrations; 93% of the cows had >1 ng/mL P4 in at least one sample. In Experiment 2, 200 lactating cows from the same farm were treated with the P4+EB+eCG and P4-Synch without eCG treatments with either a DIB or a CIDR-B (1.9 g P4; Pfizer Animal Health, Groton, CT, USA) in a 2 � 2 factorial design. Cows were examined by rectal palpation 50 days after FTAI to determine pregnancy status, and data were analyzed by chi-square Mantel-Haenszel test. There was an EB/GnRH by eCG interaction (P < 0.05) which was attributed to a higher pregnancy rate in the P4+EB+eCG group (44/98; 44.9%) than in the P4+EB without eCG group (30/100; 30.0%) and P4-Synch+eCG group (30/98; 30.6%); the P4-Synch without eCG group had an intermediate pregnancy rate that did not differ from the other treatment groups (37/98; 38.8%). In Experiment 2, no significant differences (P = 0.40) in pregnancy rates were detected between cows treated with DIB (51/100; 51.0%) or CIDR-B (42/100; 42.0%), and there was no difference (P = 0.18) between P4+EB+eCG (52/100; 52.0%) and P4-Synch without eCG (41/100; 41.0%) treated groups. Results suggest that the addition of eCG will improve pregnancy rates following FTAI in lactating dairy cows treated with EB but not in those treated with GnRH at the time of insertion and after removal of a P4 releasing device. Although treatment with P4+EB+eCG resulted in numerically higher pregnancy rates, results were not different from those obtained in the GnRH-based treatment, without the addition of eCG.

scholarly journals Prostaglandin F2α or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows

2016 ◽  
Vol 51 (6) ◽  
pp. 738-744 ◽  
Author(s):  
Luiz Francisco Machado Pfeifer ◽  
Luiz Gustavo Bruno Siqueira ◽  
Eduardo Kenji Nunes Arashiro ◽  
Natália Ávila de Castro ◽  
João Henrique Moreira Viana
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Crossbred Cows ◽  
Treatment Groups ◽  
Lactating Dairy Cows ◽  
Timed Artificial Insemination

Abstract: The objective of this work was to compare two types of ovulation inducers - estradiol benzoate (EB group) or prostaglandin F2α (PG group) -, in postpartum dairy cows subjected to timed artificial insemination (TAI). Lactating Holstein-Gir crossbred cows (n=118) with 60 to 120 days of milk production were used in this study. All cows were treated with an intravaginal progesterone-releasing device (CIDR) plus 2 mg of estradiol benzoate (EB) on day 0. The CIDR inserts were removed on day 9, and all cows received 500 µg sodium cloprostenol. Twenty-four hours after CIDR removal, cows were randomly assigned into two treatment groups and were given either 1 mg of EB i.m. (EB group) or 500 µg sodium cloprostenol i.m. (PG group). TAI were performed at 52 to 54 hours, in both EB and PG groups, after CIDR removal. No differences were observed in the ovulation (85.2% vs 72.7%) and pregnancy rates (40.7% vs 38.2%) between the EB and PG groups, respectively. Fertility is similar in the TAI protocols that use prostaglandin F2α or EB to induce ovulation in lactating dairy cows.

Post partum suboestrus in dairy cows : comparison of treatment with prostaglandin F2α or GnRH + prostaglandin F2α + GnRH

2001 ◽  
Vol 26 (2) ◽  
pp. 347-352 ◽  
Author(s):  
B. Grimard ◽  
G. Laumonnier ◽  
C. Ponsart ◽  
H. Fauxpoint ◽  
E. Barassin ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Post Partum ◽  
Prostaglandin F2α ◽  
Specific Protein ◽  
Pregnancy Rates ◽  
Progesterone Concentration ◽  
Treatment Groups ◽  
Group 2 ◽  
Group 1

AbstractTwo studies (expt. 1, 185 cows in 1996/97; expt. 2, 168 cows in 1997/98) were conducted with Prim ‘Holstein dairy cows in the Mayenne region (France) to investigate suboestrus. Cows which had not been observed in oestrus since calving were allocated alternately to treatment groups between 60 and 90 days post partum. Expt. 1. Group 1: GnRH (Day 0, 100 μg im), PGF2α (Day 7, 25 mg im), GnRH (Day 9, 100 μg im) with artificial insemination (AI) Day 10. Group 2 : PGF2α (Day 0, 25 mg im), AI at oestrus or if oestrus not observed a second PGF2α injection was given (Day 13) with AI on Day 16 and Day 17. Expt. 2. Group 1 : GnRH (Day 0, 100 /μg im), PGF2α (Day 7, 25 mg im), GnRH (Day 9, 100 μg im) with AI at observed oestrus after Day 0 or at Day 10 if oestrus not observed. Group 2 : PGF2α (Day 0, 25 mg im), AI at oestrus, or if oestrus was not observed a second PGF2α injection was given (Day 13) and AI at observed oestrus. Progesterone was measured in serum at Day 0 and in milk at AI. Pregnancy diagnosis was performed by measuring bovine Pregnancy Specific Protein B (bPSPB) (Day 50 + 3) and confirmed by ultrasonography when the result was doubtful.In expt. 1, farmers observed 47/101 (46.9 %) of group 1 cows in oestrus, 33/91 on Day 10 and 10 before Day 10. The progesterone concentrations were compatible with oestrus in 69/86 (80%) of cows on Day 10. In group 2, 36/83 (43.4 %) of cows were inseminated after the first PGF2α injection. After the second PGF2 α, only 29/43 (67 %) of cows had a low progesterone concentration at AI. The pregnancy rates were 36.1% and 32.5 % for groups 1 and 2, respectively. In expt. 2, oestrus was observed in 31/93 (33.7 %) of group 1 cows. In group 2, 51/75 (66 %) of cows were inseminated after the first injection of PGF2α, 13/75 (17.3 %) after the second and 11/75 (14.7 %) were not seen in oestrus. Pregnancy rates were 53.7% and 53.3% in groups 1 and 2, respectively. In conclusion, it is recommended that suboestrus be treated with PGF2α followed by AI at observed oestrus when oestrus detection is good while, the use of GnRH + PGF2α + GnRH is recommended when oestrus detection is poor.

scholarly journals Pregnancy rates and peripheral progesterone levels following Ovsynch or CIDR ovulation synchronization/timed artificial insemination protocols in postpartum dairy cows

2008 ◽  
Vol 88 (3) ◽  
pp. 457-461
Author(s):  
M. Aali ◽  
T. Pretheeban ◽  
G. Giritharan ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Sampling Period ◽  
Pregnancy Rates ◽  
Key Words Pregnancy ◽  
Milk Samples ◽  
Timed Artificial Insemination ◽  
Rectal Palpation

Peripheral progesterone (P4) concentrations as well as pregnancy rates (PR) between cows treated with Ovsynch or CIDR ovulation synchronization/TAI protocols were compared. Two hundred and twenty-seven postpartum lactating Holstein cows were randomly assigned to Ovsynch (n = 111) or CIDR (n = 116) ovulation synchronization/TAI protocols. Pregnancy was diagnosed by ultrasonography at day 35 after TAI and later confirmed by rectal palpation at day 60 post TAI. Milk samples were taken from the beginning of treatment (day -10) until day 35 after TAI (day 0) for P4 determination. Pregnancy rates for Ovsynch and CIDR groups of cows were 31 and 41%, respectively. The PR of ovulation synchronized cows for Ovsynch and CIDR groups were 49 and 69%, respectively. Progesterone levels during the sampling period were similar (P > 0.05) between Ovsynch and CIDR treated cows. Key words: Pregnancy rate, progesterone, Ovsynch, CIDR, cows

8 EFFECT OF SUPPLEMENTATION WITH ESTRADIOL OR ESTRADIOL PLUS GONADOTROPIN-RELEASING HORMONE AFTER PROSTAGLANDIN F2α ADMINISTRATION ON THE PREGNANCY RATES FOLLOWING TIMED ARTIFICIAL INSEMINATION IN DAIRY COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 115
Author(s):  
J. K. Jeong ◽  
H. G. Kang ◽  
I. H. Kim
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Animal Health ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Gnrh Analogue ◽  
Chi Square ◽  
Array Transducer ◽  
Timed Artificial Insemination

This study determined the effect of supplementation with oestradiol or oestradiol plus GnRH after PGF2α administration on pregnancy rates after timed artificial insemination (TAI) in dairy cows. Three hundred and six Holstein dairy cows with corpus luteum confirmed by ultrasonography (Tringa Linear with 5.0 MHz array transducer; Esaote Pie Medical, Maastricht, the Netherlands) were randomly allocated to three treatments: a single injection of 500 μg of cloprostenol, PGF2α analogue (Estrumate, Schering-Plough Animal Health, Friesoythe, Germany; PGF2α group, n = 115) designated as 0 h, an injection of 500 μg of cloprostenol and an injection of 1 mg of oestradiol benzoate (EB, SY Esrone; Samyang, Seoul, Korea) 56 h later (PGF2α + EB group, n = 114), or the same treatment as in the PGF2α + EB group and an additional injection of 100 μg of gonadorelin, GnRH analogue (Godorel, Uni-Biotech Co., Ltd., Korea) 24 h later (PGF2α + EB + GnRH group, n = 77). All cows in each group received TAI 80 h after the PGF2α injection. Pregnancy was determined at 40 to 50 days after TAI by using both ultrasonography and rectal palpation. Pregnancy rate among groups were compared by the chi-square test using the SAS program (version 9.1: SAS Institute Inc., Cary, NC, USA). Pregnancy rate following TAI were higher (P < 0.05) in the PGF2α + EB + GnRH group (34/77, 44.2%) than in the PGF2α group (31/115, 27.0%), with the PGF2α + EB group intermediate (39/114, 34.2%). In conclusion, the supplementation with oestradiol plus GnRH after PGF2α administration improved pregnancy rate after TAI in dairy cows. This work was supported by the research grant of the Chungbuk National University in 2011.

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