scholarly journals The Influence of Transglutaminase on Minced Muscular Fish Tissue Structure Formation After the Application of Various Protein Substrates

2022 ◽  
Author(s):  
Tatyana Pivnenko ◽  
Julia Karpenko ◽  
Viktoria Krashchenko

This research aimed to examine the effect of a microbial transglutaminase preparation (Activa® GS, Ajinomoto Co., Inc, Japan) on the structure formation of the myofibrillar protein system of a deep-sea fish species – the giant grenadier – after the addition of various protein substrates. The low content of proteins and their low water-holding capacity in this fish, subjected to various processing methods, leads to significant losses in the initial mass and decreased gelation ability in the muscle tissue system. Various concentrations of transglutaminase were used but these did not ensure the restructuring of the initial muscle tissue of the grenadier. Additional protein substrates with different molecular weights and amino acid composition were added, including gelatin, milk casein, hydrolysates of the skin and milt of the fish, and whole bivalves, which were used to create a firm structure. It was shown that the introduction of gelatin and casein at a concentration of 5% led to the formation of a firm, thermostable structure under the action of the enzyme, while hydrolyzed proteins with low molecular weight at their various concentrations enhanced the expression of water and formation of the fluid consistency. The ability of gastrointestinal tract proteases (pepsin and trypsin) to digest did not depend on the formation of protein-to-protein cross-linking in these combined products. The influence on the growth of the Tetrahymena pyriformis ciliates test culture also showed the high degree of product availability. The technology of molded products based on fermented minced muscle tissue of grenadier with added casein, both in the form of semi-finished products and in the form of ready-to-eat products, was developed. Keywords: transglutaminase, muscle tissue, structure formation, deep-sea fish

2018 ◽  
Vol 592 ◽  
pp. 225-242 ◽  
Author(s):  
E Cruz-Acevedo ◽  
N Tolimieri ◽  
H Aguirre-Villaseñor

2020 ◽  
pp. 75-77
Author(s):  
Ekaterina Sergeevna Krasnikova ◽  
Natalya Lvovna Morgunova ◽  
Phelix Yakovlevich Rudik ◽  
Aleksandr Vladimirovich Krasnikov ◽  
Nikita Andreevich Semilet

The results of a study of the effect of wet ultrasonic lamb meat salting on the muscle tissue microstructure are presented, and the technical parameters of the ultrasonic device are justified. It has been established that significant destruction and swelling of muscle fibers, local destruction of the sarcolemma with its pronounced rugosity are observed at ultrasonic salting with a frequency of 35 kHz, the cross-striation is poorly expressed, the tissue structure is disturbed. An ultrasonic salting with a frequency of 26 kHz was accompanied an increase in the number of transverse microcracks and crevices, loosening of muscle fibers, the formation of cavities between them while retention of the tissue structure, which contributes to the appearance of a brine between muscle fibers and accelerates its penetration into the fiber. It allows us to recommend ultrasound at a frequency of 26 kHz for cavitation activation of the brine to intensify the technological process of lamb meat salting.


Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 522
Author(s):  
Régis Santos ◽  
Wendell Medeiros-Leal ◽  
Osman Crespo ◽  
Ana Novoa-Pabon ◽  
Mário Pinho

With the commercial fishery expansion to deeper waters, some vulnerable deep-sea species have been increasingly captured. To reduce the fishing impacts on these species, exploitation and management must be based on detailed and precise information about their biology. The common mora Mora moro has become the main deep-sea species caught by longliners in the Northeast Atlantic at depths between 600 and 1200 m. In the Azores, landings have more than doubled from the early 2000s to recent years. Despite its growing importance, its life history and population structure are poorly understood, and the current stock status has not been assessed. To better determine its distribution, biology, and long-term changes in abundance and size composition, this study analyzed a fishery-dependent and survey time series from the Azores. M. moro was found on mud and rock bottoms at depths below 300 m. A larger–deeper trend was observed, and females were larger and more abundant than males. The reproductive season took place from August to February. Abundance indices and mean sizes in the catch were marked by changes in fishing fleet operational behavior. M. moro is considered vulnerable to overfishing because it exhibits a long life span, a large size, slow growth, and a low natural mortality.


Author(s):  
G. Diez ◽  
L. Arregi ◽  
M. Basterretxea ◽  
E. Cuende ◽  
I. Oyarzabal

Abstract The changes in abundance and biodiversity of deep-sea fish fauna are described based on an annual deep-water longline survey with data collected during the period 2015–2019 in the Basque Country continental Slope (ICES Division 8c). The sampling scheme included hauls in four 400 m strata, from 650–2250 m deep. The DST sensors installed in the main line have allowed us to set an accurate soak time for each haul, and they were used to calculate fishing effort and CPUE by haul. The catchability of the fishing gear indicated that 15% of the total hooks deployed in the five-year period were able to fish, and that the bottom longline was very effective in fishing a wide number of different species in all depth ranges. The fishing gear caught 14 different species of sharks (13 deepwater and one pelagic), two chimaeras and nine teleosts. The abundance and biomass registered on the hooks attached to the bottom were between three and four times higher than in the floating sections, and the highest CPUE and biomass were recorded between 1051–1450 m, from 2015 to 2017, and in the 1451–1850 m strata, but they do not show any clear trend throughout the five years of the series.


Author(s):  
Fabio C. De Leo ◽  
Jeffrey C. Drazen ◽  
Eric W. Vetter ◽  
Ashley A. Rowden ◽  
Craig R. Smith

Author(s):  
Phillip J Turner ◽  
Matthew Gianni ◽  
Ellen Kenchington ◽  
Sebastian Valanko ◽  
David E Johnson

Abstract The European Union’s deep-sea fisheries regulations (Regulation (EU) No. 2016/2336) established obligations to manage deep-sea fisheries and to protect vulnerable marine ecosystems (VMEs). The European Commission is scheduled to complete a review of the regulations in 2021, providing an opportunity for new scientific information to be incorporated into the implementation of the regulations. Here, we summarise research outputs from the EU-funded Horizon 2020 ATLAS Project and explain their relevance to the regulation of deep-sea fisheries in EU waters. ATLAS research has increased our understanding of the distribution of VMEs and their importance in terms of ecosystem functioning. ATLAS research has also highlighted the utility of molecular techniques to understand fish population structure and the potential for habitat suitability models to help incorporate climate change into decision-making. Building on these scientific advances, we provide recommendations to help increase the effectiveness of management measures to conserve deep-sea fish stocks and protect VMEs.


2021 ◽  
Author(s):  
Lucía Soliño ◽  
Leticia Vidal-Liñán ◽  
Patricia Pérez-Pérez ◽  
Salvador García-Barcelona ◽  
Francisco Baldó ◽  
...  

2018 ◽  
Vol 2 (4) ◽  
pp. 680-687 ◽  
Author(s):  
Michelle R. Gaither ◽  
Georgios A. Gkafas ◽  
Menno de Jong ◽  
Fatih Sarigol ◽  
Francis Neat ◽  
...  

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