scholarly journals Latex Agglutination Assay- A Rapid Test to Diagnose Rotavirus Infection

2017 ◽  
Vol 05 (04) ◽  
pp. 20802-20806
Author(s):  
Archana Sharma ◽  
Keyword(s):  
Rapid Test ◽  
Latex Agglutination ◽  
Agglutination Assay ◽  
Rotavirus Infection ◽  
Latex Agglutination Assay

Toxin Production by Psychrotrophic Bacillus spp. Present in Milk

1990 ◽  
Vol 53 (9) ◽  
pp. 790-792 ◽  
Author(s):  
M. W. GRIFFITHS
Keyword(s):  
Bacillus Cereus ◽  
Brain Heart Infusion ◽  
Bacterial Count ◽  
Toxin Production ◽  
Latex Agglutination ◽  
Brain Heart Infusion Broth ◽  
Agglutination Assay ◽  
Bacillus Spp ◽  
Latex Agglutination Assay

Using a reversed passive latex agglutination assay, about 85% of psychrotrophic Bacillus spp. tested were shown to produce diarrhoegenic toxin during growth on brain heart infusion broth at 25°C. The majority of these strains were identified as Bacillus cereus or cereus-related strains. However, a number of other species was capable of synthesizing the toxin. Further investigation of four psychrotrophic Bacilli showed that the toxin was produced during growth in milk at temperatures ranging from 6 to 21°C. Toxin production increased with increasing temperatures and was not synthesized in appreciable quantities until the bacterial count exceeded 1 × 107 cfu/ml.

Listeriolysin O-Based Latex Agglutination Test for the Rapid Detection of Listeria monocytogenes in Foods

1997 ◽  
Vol 60 (9) ◽  
pp. 1038-1040 ◽  
Author(s):  
GHASSAN M. MATAR ◽  
PEGGY S. HAYES ◽  
WILLIAM F. BIBB ◽  
BALA SWAMINATHAN
Keyword(s):  
Monoclonal Antibody ◽  
Listeria Monocytogenes ◽  
Rapid Detection ◽  
Rapid Test ◽  
Food Samples ◽  
Cross Reactivity ◽  
Latex Agglutination ◽  
Listeriolysin O ◽  
Agglutination Assay ◽  
Latex Beads

A latex agglutination-based test for the rapid detection of Listeria monocytogenes in foods was developed. An antilisteriolysin O (LLO) monoclonal antibody (HID5E12D7; IgG2b) covalently bound to polystyrene amidine-modified latex beads was used in a slide agglutination assay. The latex reagent detected 0.1 ng/ml of LLO in phosphate-buffered saline plus bovine serum albumin. It reacted with culture supernatants of L. monocytogenes but not with other Listeria species or Streptococcus groups A through G. The listeriolysin O latex agglutination assay (LLOLAT) was applied to 24-h and 48-h USDA primary enrichment cultures of 208 food samples obtained from refrigerators of listeriosis patients enrolled in a study to determine the role of foods in sporadic listeriosis. Of 19 samples positive by cultural techniques, 17 were positive by the LLOLAT. Cultures with low (<0.3 CFU/g) levels of L. monocytogenes were positive in the LLOLAT. No cross-reactivity occurred when using a heterogeneous monoclonal antibody. The LLOLAT is a sensitive, specific and rapid test and may be useful for screening foods for L. monocytogenes.

A Rapid and Reliable Method for the Detection of Molds in Foods: Using the Latex Agglutination Assay

1989 ◽  
Vol 52 (4) ◽  
pp. 244-247 ◽  
Author(s):  
H. J. KAMPHUIS ◽  
S. NOTERMANS ◽  
G. H. VEENEMAN ◽  
J. H. VAN BOOM ◽  
F. M. ROMBOUTS
Keyword(s):  
Reliable Method ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Penicillium Digitatum ◽  
Agglutination Assay ◽  
Culture Filtrates ◽  
Latex Beads ◽  
Positive Reactivity ◽  
Latex Agglutination Assay

An agglutination test by using latex beads (0.8 μm diameter) coated with IgG from the antibodies against extra-cellular polysaccharide of Penicillium digitatum has been developed. As low as 5 to 10 ng/ml of the purified extra-cellular polysaccharide of the same species can be detected by this preparation. Analysis of culture filtrates from 25 different molds showed that the positive reactivity was obtained only with the species of genera Penicillium and Aspergillus. The application of this test was confirmed in testing the samples of spices and nuts. Further, the reliability could be enhanced by including specific blocker in the assay, the synthetic epitopes consisting of four β (1–5)-linked D-galactofuranosyl residues.

Automated enhanced latex agglutination assay for rheumatoid factors in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 303-307 ◽  
Author(s):  
J W Winkles ◽  
J Lunec ◽  
L Gray
Keyword(s):  
Rheumatoid Arthritis ◽  
Latex Agglutination ◽  
Rheumatoid Factors ◽  
Agglutination Assay ◽  
Sample Throughput ◽  
Wide Range ◽  
Latex Agglutination Assay

Abstract This improved assay of rheumatoid factors in serum, described here for use with the Baker "Encore" centrifugal analyzer, is efficient, with 250-sample throughput per hour; reproducible, with between-batch CV = 5% and within-batch CV = 2% (mid-assay range); and results correlate well (r = 0.9) with those by other methods. The method is fully quantitative and automated, involves no predilution steps, and can be adapted for use in a wide range of systems. It has a sensitivity of 96% and specificity of 80% in diagnosing rheumatoid arthritis.

Combination of MALDI-TOF MS and PBP2′ latex agglutination assay for rapid MRSA detection

2018 ◽  
Vol 144 ◽  
pp. 122-124 ◽  
Author(s):  
Marianna Ábrók ◽  
Andrea Lázár ◽  
Mária Szécsényi ◽  
Judit Deák ◽  
Edit Urbán
Keyword(s):  
Latex Agglutination ◽  
Agglutination Assay ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms ◽  
Latex Agglutination Assay

Spread of a large plasmid carrying thecpegene and thetcplocus amongstClostridium perfringensisolates from nosocomial outbreaks and sporadic cases of gastroenteritis in a geriatric hospital

2008 ◽  
Vol 137 (1) ◽  
pp. 108-113 ◽  
Author(s):  
S. KOBAYASHI ◽  
A. WADA ◽  
S. SHIBASAKI ◽  
M. ANNAKA ◽  
H. HIGUCHI ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Latex Agglutination ◽  
Pulsed Field Gel Electrophoresis ◽  
Large Plasmid ◽  
Pcr Assay ◽  
Agglutination Assay ◽  
Geriatric Hospital ◽  
Sporadic Cases ◽  
Hospital Wards ◽  
Latex Agglutination Assay

SUMMARYTo investigate two clusters of diarrhoea cases observed in our geriatric hospital wards, the faecal specimens were analysed. Reversed passive latex agglutination assay revealed that 63·2% and 41·7% of the faecal specimens from each cluster were positive forClostridium perfringensenterotoxin. PCR assay revealed that 71·4% and 68·8% ofC. perfringensisolates from each cluster were positive for the enterotoxin gene (cpe). These observations suggested that both the clusters were outbreaks caused by enterotoxigenicC. perfringens. Subsequent pulsed-field gel electrophoresis analysis revealed that the two outbreaks were caused by differentC. perfringensisolates. However, these outbreak isolates as well as other sporadic diarrhoea isolates shared a 75-kb plasmid on which thecpegene and thetcplocus were located. The 75-kb plasmid had horizontally spread to variousC. perfringensisolates and had caused outbreaks and sporadic infections. However, the site and time of the plasmid transfer are unclear.

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