scholarly journals Curcumin blocks autophagy and activates apoptosis of malignant mesothelioma cell lines and increases the survival of mice intraperitoneally transplanted with a malignant mesothelioma cell line

Oncotarget ◽  
2017 ◽  
Vol 8 (21) ◽  
pp. 34405-34422 ◽  
Author(s):  
Laura Masuelli ◽  
Monica Benvenuto ◽  
Enrica Di Stefano ◽  
Rosanna Mattera ◽  
Massimo Fantini ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Malignant Mesothelioma ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell

scholarly journals β-catenin inhibits cell growth of a malignant mesothelioma cell line, NCI-H28, with a 3p21.3 homozygous deletion

Oncogene ◽  
2003 ◽  
Vol 22 (39) ◽  
pp. 7922-7930 ◽  
Author(s):  
Noriyasu Usami ◽  
Yoshitaka Sekido ◽  
Osamu Maeda ◽  
Kazuhito Yamamoto ◽  
John D Minna ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Malignant Mesothelioma ◽  
Homozygous Deletion ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell

Establishment and Characterization of a Human Malignant Mesothelioma Cell line (HMMME)

Human Cell ◽  
2003 ◽  
Vol 16 (4) ◽  
pp. 231-239 ◽  
Author(s):  
Isamu ISHIWATA ◽  
Chieko ISHIWATA ◽  
Mitsuru ANZO ◽  
Rie MINAMI ◽  
Kazushige KIGUCHI ◽  
...  
Keyword(s):  
Cell Line ◽  
Malignant Mesothelioma ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell

scholarly journals A subset of microRNAs defining the side population of a human malignant mesothelioma cell line

Oncotarget ◽  
2017 ◽  
Vol 8 (26) ◽  
pp. 42847-42856 ◽  
Author(s):  
Myung-Chul Kim ◽  
Na-Yon Kim ◽  
Yu-Ri Seo ◽  
Yongbaek Kim
Keyword(s):  
Cell Line ◽  
Malignant Mesothelioma ◽  
Side Population ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell

844 Immunomodulatory activity of epigenetic drugs combinations in mesothelioma: laying the ground for new immunotherapeutic strategies

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A896-A896
Author(s):  
Sara Cannito ◽  
Health Biology ◽  
Ornella Cutaia ◽  
Carolina Fazio ◽  
Maria Fortunata Lofiego ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Hla Class I ◽  
Class I ◽  
Immunomodulatory Activity ◽  
Epigenetic Drugs ◽  
Hla Class I Antigens ◽  
Hla Class I Molecules ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell

BackgroundGrowing evidence are demonstrating the therapeutic efficacy of immune checkpoint inhibitors (ICI) in mesothelioma; however, a limited percentage of patients benefits from this therapeutic approach. Epigenetic modifications play a relevant role in negatively regulating the cross-talk between neoplastic and immune cells, and in contributing to the highly immunosuppressive mesothelioma microenvironment. A better understanding of mesothelioma epigenetic landscape could open the path to novel and potentially more effective approaches combining ICI and epigenetic drugs. We investigated the immunomodulatory potential of epigenetic agents by comparing the activity of DNA hypomethylating agents (DHA) with histone deacetylases inhibitors (HDACi) and EZH2 inhibitors (EZH2i), alone or combined with DHA, in mesothelioma cells.MethodsFour mesothelioma cell lines were treated with the DHA guadecitabine 1μM, or with the HDACi, Valproic Acid (VPA) 1mM, or the EZH2i, EPZ-6438 1μM, alone or combined with guadecitabine. We investigated the expression of HLA class I molecules by flow-cytometry and of PD-L1, cancer testis antigens (CTA: NY-ESO, MAGE-A1), Natural Killer Group 2 member D Ligands (NKG2DLs: MIC-A, MIC-B, ULBP2) and EMT-regulating cadherins (CDH1, CDH2) by quantitative Real-Time PCR. Fold change (FC) expression for each treatment vs untreated cells was reported as mean values (FCm) among investigated cell lines. A positive modulation of the expression was considered if FCm>1.5.ResultsGuadecitabine upregulated the expression of HLA class I antigens (FCm=1.75), PD-L1 (FCm=2.38), NKG2DLs (MIC-A FCm=1.96, MIC-B FCm=2.57, and ULBP2 FCm=3.56), and upregulated/induced CTA expression. Similarly, VPA upregulated HLA class I antigens (FCm=1.67), PD-L1 (FCm=3.17), NKG2DLs (MIC-A FCm=1.78, MIC-B FCm=3.04, and ULBP2 FCm=3.75) expression; however, CTA expression was modulated only in 1 mesothelioma cell line. Conversely, EPZ-6438 up-regulated only NY-ESO-1 and MIC-B expression in 1 mesothelioma cell line.The addition of both VPA and EPZ-6483 to guadecitabine strengthened its immunomodulatory activity. Specifically, guadecitabine plus VPA or EPZ-6438 upregulated the expression of HLA class I antigens FCm=2.55 or 2.69, PD-L1 FCm=8.04 or 2.65, MIC-A FCm=3.81 or 2.26, MIC-B FCm=8.00 or 3.03, ULBP2 FCm=6.24 or 4.53, respectively. Higher levels of CTA upregulation/induction were observed with combination treatments vs guadecitabine alone.Cadherins modulation was mesothelioma histotype-related: CDH1 expression was induced in the 2 constitutively-negative sarcomatoid mesothelioma cells by guadecitabine alone or combined with VPA or EPZ-6438; CDH2 expression was upregulated by VPA alone (FCm=1.53) or plus guadecitabine (FCm=2.54).ConclusionsCombination of DHA-based immunotherapies with other classes of epigenetic drugs could be an effective strategy to be pursued in the mesothelioma clinic.

First Molecular Cytogenetic Characterization of Murine Malignant Mesothelioma Cell Line AE17 and In Silico Translation to the Human Genome

2017 ◽  
Vol 12 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Eva Kubicova ◽  
Vladimir Trifonov ◽  
Fran Borovecki ◽  
Thomas Liehr ◽  
Martina Rincic ◽  
...  
Keyword(s):  
Human Genome ◽  
Cell Line ◽  
Malignant Mesothelioma ◽  
In Silico ◽  
Molecular Cytogenetic ◽  
Mesothelioma Cell Line ◽  
Cytogenetic Characterization ◽  
Mesothelioma Cell

scholarly journals Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

1992 ◽  
Vol 200 (1) ◽  
pp. 83-88 ◽  
Author(s):  
M.A. Versnel ◽  
M.J. Bouts ◽  
A.W. Langerak ◽  
Th. H. vander Kwast ◽  
H.C. Hoogsteden ◽  
...  
Keyword(s):  
Cell Line ◽  
Malignant Mesothelioma ◽  
Receptor Expression ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell ◽  
Β Receptor

scholarly journals Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1034 ◽  
Author(s):  
Ashish Kumar-Singh ◽  
Jatin Shrinet ◽  
Malgorzata Maria Parniewska ◽  
Jonas Fuxe ◽  
Katalin Dobra ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Cell Line ◽  
Rna Splicing ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Mesothelioma Cell Line ◽  
Mesothelioma Cell ◽  
And Migration ◽  
Dependent Transport

Syndecan-1 (SDC1) is a cell surface heparan sulfate proteoglycan (HSPG), which regulates various signaling pathways controlling the proliferation and migration of malignant mesothelioma and other types of cancer. We have previously shown that SDC1 can translocate to the nucleus in mesothelioma cells through a tubulin-dependent transport mechanism. However, the role of nuclear SDC1 is largely unknown. Here, we performed co-immunoprecipitation (Co-IP) of SDC1 in a mesothelioma cell line to identify SDC1 interacting proteins. The precipitates contained a large number of proteins, indicating the recovery of protein networks. Proteomic analysis with a focus on nuclear proteins revealed an association with pathways related to cell proliferation and RNA synthesis, splicing and transport. In support of this, the top RNA splicing candidates were verified to interact with SDC1 by Co-IP and subsequent Western blot analysis. Further loss- and gain-of-function experiments showed that SDC1 influences RNA levels in mesothelioma cells. The results identify a proteomic map of SDC1 nuclear interactors in a mesothelioma cell line and suggest a previously unknown role for SDC1 in RNA biogenesis. The results should serve as a fundament for further studies to discover the role of nuclear SDC1 in normal and cancer cells of different origin.

Expression of c- in human malignant mesothelioma cell lines

1987 ◽  
Vol 23 (11) ◽  
pp. 1809
Author(s):  
M.A. Versnel ◽  
H.C. Hoogstedon ◽  
M.J. Bouts ◽  
Th.H. van der Kwast ◽  
A. Hagemeijer
Keyword(s):  
Cell Lines ◽  
Malignant Mesothelioma ◽  
Mesothelioma Cell
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