scholarly journals Virus-Free Plant Regeneration from Shoot Apical Meristem of Coccinia grandis L., an Important Medicinal Plant in Bangladesh

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Md. F. Hasan ◽  
Mst M. Jannat ◽  
Rashed Zaman ◽  
Biswanath Sikdar ◽  
◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Meristem Culture ◽  
Root Induction ◽  
Ms Medium ◽  
Coccinia Grandis

This investigation was undertaken to establish an efficient protocol for virus-free plant regeneration in Coccinia grandis L. through shoot apical meristem culture. Murashige and Skoog (MS) basal medium supplemented with different concentrations and combinations of 6-benzyl amino purine (BAP), gibberellic acid (GA3), naphthalene acetic acid (NAA), and indole-3-butyric acid (IBA) was used for meristem establishment, shoot regeneration, and root induction as well as elongation. MS liquid medium supplemented with 1.0 mg l-1 BAP + 0.10 mg l-1 NAA was found to be the best medium for the primary establishment of meristems. MS medium containing 1.5 mg l-1 BAP + 0.5 mg l-1 GA3 + 0.5 mg l-1 NAA was found to be best for shoot regeneration percentage at 100.0 ± 0.0 and multiplication with 10.0 ± 0.8 shoots per meristem as well as shoot elongation (highest 9.0 ± 0.0 cm). In vitro grown shoots were subcultured and rooted with 11.0 ± 0.8 roots per shoot subsequently on MS medium containing 0.5 mg l-1 IBA. Well-rooted plantlets were gradually acclimatized and successfully established in the field condition with 100% survival rate.

scholarly journals The regeneration potential of promising winter common wheat lines in shoot apical meristem culture

2019 ◽  
Vol 25 ◽  
pp. 298-303
Author(s):  
S. V. Pykalo ◽  
O. A. Demydov ◽  
T. V. Yurchenko ◽  
N. I. Prokopik ◽  
O. V. Humeniuk
Keyword(s):  
Common Wheat ◽  
Shoot Regeneration ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Plant Production ◽  
Meristem Culture ◽  
Culture Methods ◽  
Regeneration Potential ◽  
Regenerated Plants ◽  
Wheat Lines

Aim. To investigate the regenerative ability of promising winter common wheat lines in shoot apical meristem culture. Methods. Plant tissue culture methods, statistical evaluation of data. Results. The processes of morphogenesis in culture of apical meristem of 3-days seedlings of lines of winter common wheat were investigated and it was established that the frequency of callusogenesis and shoot regeneration in the studied forms is determined by the genotype of explant. Two types of callus with morphophysiological properties were identified: morphogenic and nonmorphogenic callus. The formation of regenerated plants from wheat calli took place through both gemmorizogenesis and somatic embryogenesis. Conclusions. The line Erytrospermum 60068 was characterized the highest regeneration potential and it can be recommended for further biotechnology of wheat. Obtained technology of vigorous regenerated plant production of winter common wheat lines in shoot apical meristem culture can be used in cell selection and genetic engineering experiments. Keywords: winter common wheat, apical meristem, genotype, callus, shoot regeneration.

Plant regeneration from stem callus of cassava

1979 ◽  
Vol 57 (16) ◽  
pp. 1761-1763 ◽  
Author(s):  
J. P. Tilquin
Keyword(s):  
Plant Regeneration ◽  
Gibberellic Acid ◽  
Shoot Apical Meristem ◽  
Manihot Esculenta ◽  
Apical Meristem ◽  
Callus Formation ◽  
Shoot Development ◽  
Meristem Culture ◽  
Manihot Esculenta Crantz ◽  
Leaf Differentiation

Callus formation and organogenesis have been induced on the internode culture of cassava (Manihot esculenta Crantz). Callusing was rapidly induced on the medium devised by Kartha, Gamborg, and Constabel for the shoot apical meristem culture of cassava. During culture, green protuberances appear on the callus followed by the differentiation of a leaf-like structure. The leaf-like structure degenerated after a month of differentiation. On the same medium, but lacking gibberellic acid (GA3), organogenesis is less frequent and precocious but the leaves which appear are typical of cassava; leaf differentiation is followed by shoot development.

scholarly journals In vitro plant regeneration of lotus (Nelumbo nucifera)

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Xia Yu ◽  
Jiajing Sheng ◽  
Lingling Zhao ◽  
Ying Diao ◽  
Xingwen Zheng ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Nelumbo Nucifera ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
In Vitro Plant Regeneration ◽  
Regeneration System ◽  
Indolebutyric Acid

AbstractIn this study, an efficient and reproducible plant regeneration system for lotus (Nelumbo nucifera Gaertn.) was established using shoot apical meristems from the buds and one-week-old aseptically germinated embryos as explants. Multiple shoot clumps were induced on Murashige and Skoog (MS) basal medium supplemented with various combinations of N6-Benzylaminopurine (6-BA) and α-Naphthalene acetic acid (NAA). The maximum response was obtained with 2.22 μM 6-BA, and produced 21.33 shoots per explant after four weeks. After five subcultures, multiple shoot clumps were transferred to MS basal medium supplemented with various combinations of 3-Indolebutyric acid (IBA), NAA and sucrose for root induction. After four weeks, plantlets with well-developed roots were achieved on MS basal medium supplemented with 0.54 μM NAA and 30 g/L sucrose with 100% rooting rate. The successfully acclimated plantlets were transferred to pots with the addition of 2 g/L KMnO4 into the soils, and finally fertile plants with much bigger leaves were obtained in the greenhouse. The survival rate was 97.33%.

scholarly journals In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species

2020 ◽  
Vol 12 (1) ◽  
pp. 74-89
Author(s):  
Michael S. AKINROPO ◽  
Benjamin E. AYISIRE ◽  
Ejeoghene R. OGBIMI
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Enterolobium Cyclocarpum

This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination.  The leaf explants did not respond to these treatments.  The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus.  The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone.  Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively.  On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.

scholarly journals Shoot regeneration and root induction in brinjal by growth regulators

2015 ◽  
Vol 2 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Md Abdul Alim ◽  
Bhabendra Kumar Biswas ◽  
Md Hasanuzzaman ◽  
Pronay Bala ◽  
Santanu Roy
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Maximum Rate ◽  
Root Formation ◽  
Solanum Melongena ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Overall Performance

The experiment was carried out to study the effect of genotypes and growth regulators on root induction and shoot regeneration of brinjal (Solanum melongena) genotypes. Leaf segments of three varieties of Solanum were cultured on MS medium with different concentrations and combinations of plant growth regulators. Among the tested genotypes, Protab showed highest percentage of shoot regeneration (65.67%). Early and maximum rate of regeneration was found in MS + 1 mg/L NAA (naphthalene acetic acid) + 0.1 mg/LBAP (6-benzylamino purine) for all the genotypes. The highest number of roots per shoot was counted in Protab (73.33%) on ½MS + 2 mg/L IBA (indole butyric acid) + 0.4 mg/L BAP. Based on the overall performance, the variety Protab appeared as the best for shoot and root formation and ultimately successful regeneration of plants.Res. Agric., Livest. Fish.2(1): 43-51, April 2015

scholarly journals Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species

HortScience ◽  
2007 ◽  
Vol 42 (7) ◽  
pp. 1670-1673 ◽  
Author(s):  
Wenhao Dai ◽  
Cielo Castillo
Keyword(s):  
Shoot Regeneration ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Dark Treatment ◽  
Regeneration Rate ◽  
Factors Affecting ◽  
In Vitro Shoots ◽  
Leaf Tissues

The effects of genotype, basal medium, plant growth regulator (PGR), dark treatment, and antibiotics on shoot regeneration of two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were investigated. In vitro shoots were regenerated from leaf tissues in either Murashige and Skoog (MS) or woody plant medium (WPM) media supplemented with benzyladenine (BA). In general, more shoots were regenerated in WPM medium than in MS medium. Dark treatment for 3 to 5 weeks dramatically increased shoot regeneration. Addition of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) significantly enhanced the regeneration rate and shoots of each explant. The maximum regeneration rate (100%) of B. davidii ‘Potters Purple’ was achieved when cultured in WPM containing 5 μm BA plus 5 μm IBA. The maximum regeneration rate (98.4%) of Buddleia ‘Lochinch’ was found in WPM supplemented with 20 μm BA plus 4 μm IBA. Carbenicillin at 250 to 500 mg·L−1 and cefotaxime at 125 to 250 mg·L−1, individually or combined, promoted shoot regeneration. Interactions between genotype and medium or PGRs were found. In vitro shoots were easily rooted in half-strength MS medium with or without NAA. Rooted plants were transferred to potting mix and grown in the greenhouse. This research will facilitate genetic improvement and fast propagation of Buddleia species using biotechnology.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Callus induction and plant regeneration in the moss Aloina Aloides (Schultz) Kindb. (Pottiaceae, Bryopsida)

2003 ◽  
Vol 55 (3-4) ◽  
pp. 77-80 ◽  
Author(s):  
Aneta Bijelovic ◽  
Marko Sabovljevic
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Air Humidity ◽  
Moss Species ◽  
Bud Formation ◽  
Long Period ◽  
2,4 Dichlorophenoxyacetic Acid

Callus induction of moss species Aloina aloides (Schultz) Kindb. was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin (KIN) or with 0.2 mg/L indole-3-butyric acid (IBA) and 2.0 mg/L 6-benzylaminopurine (BAP) or with 7.5 g/L of sucrose or with 15 g/L of sucrose or hormone - free and sugar free MS basal medium. The callus can be maintained for a long period of time without bud formation subcultured on the above media, at 16 h day/8 h night, 25 ? 2?C, 60-70% air humidity and irradiance of 50 ?mol m-2s-1. To obtain plant regeneration pieces, calli were transferred onto MS media supplemented with different concentrations of auxins and cytokinins (1.0 mg/L 2,4-D and 2 mg/L KIN; 0.2 mg/L IBA and 2 mg/L KIN; or 0.2 mg/L IAA and 2 mg/L BAP). In these media after subculturing, callus enlarges and turns to gametophytes with buds. Except for a smaller size, the plants obtained on the callus did not differ morphoanatomically from the shoots in the nature.

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