Bromatometric Estimation of Irbesartan, Losartan, and Bisoprolol in Bulk and Tablet Forms: Statistical Analysis

A new spectrophotometric method is described for determination of irbesartan, losartan and bisoprolol in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and rhodamine B as chromogenic agent. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of rhodamine B then measuring absorbance at 557nm. The amount of bromine reacted corresponds to the amount of each drug. Effect of acidity, bromate-bromide volume and reaction time, on the absorption were studied. Calibration curves were linear over ranges of and for irbesartan, losartan, and bisoprolol, respectively. The method was satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.

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Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.21981 ◽

2015 ◽

Vol 12 (2) ◽

pp. 171-179 ◽

Cited By ~ 3

Author(s):

Nahid Sharmin ◽

Nazia Sultana Shanta ◽

Sitesh C Bachar

Keyword(s):

Statistical Analysis ◽

Spectrophotometric Method ◽

Dosage Form ◽

Dosage Forms ◽

Spectrophotometric Analysis ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Ich Guidelines ◽

Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

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Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

E-Journal of Chemistry ◽

10.1155/2012/757916 ◽

2012 ◽

Vol 9 (1) ◽

pp. 89-92

Author(s):

J. Sudhakar Reddy ◽

MD. S. Maqsood Ahmed ◽

I. E. Chakravarthy ◽

K. Prabhavathi

Keyword(s):

Statistical Analysis ◽

Dosage Form ◽

Dosage Forms ◽

Ion Pair ◽

Reagent Blank ◽

Pharmaceutical Dosage Forms ◽

Extractable Complex ◽

Bulk Drug ◽

Tablet Dosage

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

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Development and Validation of a RP-HPLC Method for Simultaneous Determination of Levofloxacin and Moxifloxacin in Pharmaceutical Dosage Forms

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.17614 ◽

2014 ◽

Vol 12 (2) ◽

Author(s):

Farjahan Nur ◽

Asma Rahman ◽

Md. Zakir Sultan ◽

Md. Gias Uddin ◽

Mohammad A Rashid

Keyword(s):

Simultaneous Determination ◽

Dosage Forms ◽

Hplc Method ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc ◽

Development And Validation

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Spectrophotometeric Determination of Trifluoperazine HCL in Pure Forms and Pharmaceutical Preperations

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8593 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Mohammad Hamzah Hamzah ◽

Rawa M M Taqi ◽

Muna M. Hasan ◽

Raid J. M. Al-Timimi

Keyword(s):

Standard Method ◽

Limit Of Detection ◽

Molar Absorptivity ◽

Dosage Forms ◽

Oxidizing Agent ◽

Optimum Conditions ◽

Limit Of Quantification ◽

Cerium Ion ◽

Blank Solution

A simple and accurate spectrophotometric method for the determination of Trifluoperazine HCl in pure and dosage forms was developed. The method is based on the reaction between Trifluoperazine HCl and p-chloroaniline in the presence of cerium ion as oxidizing agent which lead to the formation of violate color product that absorbed at a maximum wavelength 570nm while the blank solution was pink. Under the optimum conditions a linear relationship between the intensity and concentration of TRF in the range 4-50μg/ml was obtained . The molar absorptivity 3.74×103 L.mol-1.cm-1 , Limit of detection (2.21μg/ml), while limit of quantification was 7.39μg/ml. The proposed analytical method was compared with standard method using t-test and F-test , the obtained results shows there is no significant differences between proposed method and standard method. Based on that the proposed method can be used as an alternative method for the determination of TRF in pure and dosage forms.

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Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.4.7 ◽

2012 ◽

Vol 4 (4) ◽

pp. 1563-1568

Author(s):

Sagar Suman Panda ◽

Ravi Kumar B V V ◽

D Patanaik

Keyword(s):

Spectrophotometric Method ◽

Absorption Maximum ◽

Dosage Form ◽

Dosage Forms ◽

Alendronate Sodium ◽

Colored Complex ◽

Pharmaceutical Dosage Forms ◽

Recovery Study ◽

Assay Conditions

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.

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Electroanalytical Methods for Determination of Calcium Channel Blockers

Current Analytical Chemistry ◽

10.2174/1573411014666180426165750 ◽

2019 ◽

Vol 15 (3) ◽

pp. 207-218 ◽

Cited By ~ 1

Author(s):

Fatma Ağın

Keyword(s):

Calcium Channel ◽

Calcium Channel Blockers ◽

Channel Blocker ◽

Heart Diseases ◽

Dosage Forms ◽

Channel Blockers ◽

Electroanalytical Methods ◽

Pharmaceutical Dosage Forms ◽

Ischemic Heart Diseases

Background:Calcium Channel Blockers (CCBs) are widely used in the treatment of cardiovascular and ischemic heart diseases in recent years. They treat arrhythmias by reducing cardiac cycle contraction and also benefit ischemic heart diseases. Electroanalytical methods are very powerful analytical methods used in the pharmaceutical industry because of the determination of therapeutic agents and/or their metabolites in clinical samples at extremely low concentrations (10-50 ng/ml). The purpose of this review is to gather electroanalytical methods used for the determination of calcium channel blocker drugs in pharmaceutical dosage forms and biological media selected mainly from current articles.Methods:This review mainly includes recent determination studies of calcium channel blockers by electroanalytical methods from pharmaceutical dosage forms and biological samples. The studies of calcium channel blockers electroanalytical determination in the literature were reviewed and interpreted.Results:There are a lot of studies on amlodipine and nifedipine, but the number of studies on benidipine, cilnidipine, felodipine, isradipine, lercanidipine, lacidipine, levamlodipine, manidipine, nicardipine, nilvadipine, nimodipine, nisoldipine, nitrendipine, diltiazem, and verapamil are limited in the literature. In these studies, DPV and SWV are the most used methods. The other methods were used less for the determination of calcium channel blocker drugs.Conclusion:Electroanalytical methods especially voltammetric methods supply reproducible and reliable results for the analysis of the analyte. These methods are simple, more sensitive, rapid and inexpensive compared to the usually used spectroscopic and chromatographic methods.

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