scholarly journals ESTABELECIMENTO IN VITRO DE DUAS ESPÉCIES DE BAMBU: Dendrocalamus asper (Schultes f.) Backer ex Heyne E Bambusa oldhamii Munro

2015 ◽  
pp. 1172-1182
Author(s):  
Cris Hanny Pires Araujo ◽  
Luciano Lajovic Carneiro ◽  
Cristhian Lorraine Pires Araujo ◽  
Sérgio Tadeu Sibov
Keyword(s):  
Dendrocalamus Asper ◽  
Bambusa Oldhamii

scholarly journals In vitro establishment of Bambusa oldhamii Munro from field-grown matrices and molecular identification of endophytic bactéria

2019 ◽  
Vol 49 ◽  
Author(s):  
Ana Paula de Azevedo Pasqualini ◽  
Gabriela Xavier Schneider ◽  
Hugo Pacheco de Freitas Fraga ◽  
Luiz Antonio Biasi ◽  
Marguerite Quoirin
Keyword(s):  
Molecular Identification ◽  
Culture Medium ◽  
Bacterial Isolate ◽  
Fungal Growth ◽  
Endophytic Bacterium ◽  
Liquid Culture Medium ◽  
Rdna Sequencing ◽  
In Vitro Establishment ◽  
Bambusa Oldhamii

ABSTRACT In plant micropropagation, the establishment stage is difficult, due to the presence of microorganisms in tissues from field-grown matrices, especially for bamboo. This study aimed to establish an efficient asepsis protocol for Bambusa oldhamii explants from field plants, as well as to carry out the molecular identification of a possible endophytic bacterial isolate. The explants were exposed to 70 % alcohol, 1 % sodium hypochlorite (NaOCl), 0.1 % mercuric chloride (HgCl2), thiophanate-methyl (Cercobin®) and chlorhexidine digluconate (2 % Riohex®) in different combinations, and introduced into Murashige and Skoog culture medium (solid or liquid), supplemented or not with 4 mL L-1 of Plant Preservative Mixture (PPMTM), totaling seven treatments. The asepsis and immersion of the explants in the liquid culture medium containing 4 mL L-1 of PPMTM visually inhibited the bacterial and fungal growth, allowed the development of shoots with a mean length of 2.2 cm and posterior subcultures, being the best treatment used for the in vitro establishment of B. oldhamii. The molecular identification of an endophytic bacterium performed by 16S rDNA sequencing allowed to identify the bacterial isolate as Ralstonia sp., with 100 % of similarity, and the phylogenetic analysis grouped it with Ralstonia pickettii. In addition, the bacterial isolate showed to be sensitive to 4 mL L-1 of PPMTM by the minimum inhibitory concentration test.

scholarly journals Effects of a commercial biocide, kasugamycin and consistency of the culture medium on the in vitro establishment of bamboo1

2019 ◽  
Vol 49 ◽  
Author(s):  
Daniela Werner Ribeiro dos Santos ◽  
Théo Piucco Rocker ◽  
Thiago Sanches Ornellas ◽  
Miguel Pedro Guerra
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
Liquid Culture Medium ◽  
Bambusa Vulgaris ◽  
Phyllostachys Bambusoides ◽  
In Vitro Establishment ◽  
Dendrocalamus Asper

ABSTRACT The contamination by microorganisms and oxidation of explants in the in vitro establishment of bamboo are recurrent problems for its micropropagation. In the present study, effects of the biocide Plant Preservative Mixture (PPM™), the antibiotic kasugamycin and the consistency of the culture medium were evaluated in the in vitro establishment of Bambusa vulgaris,Phyllostachys bambusoides and Dendrocalamus asper. The presence of PPM™ in the culture medium had a significant effect using 2 mL L-1 or 4 mL L-1 concentrations, as well as in the liquid culture medium, increasing the plants established in the autumn. Kasugamycin promoted variable responses for all the three species, depending on the season. There was interaction among the factors, demonstrating that higher rates of viable plants can be obtained by combining different strategies to reduce the oxidation and contamination. For the in vitro establishment of B. vulgaris,P. bambusoides and D. asper, it is recommended to add 2 mL L-1 or 4 mL L-1 of PPM™ to the liquid culture medium.

scholarly journals In vitro culture and diversity of endophytic fungi in Bambusa oldhamii

2019 ◽  
Vol 49 ◽  
Author(s):  
Ana Paula de Azevedo Pasqualini ◽  
Mariely Cristine dos Santos ◽  
Bruno Francisco Sant´Anna-Santos ◽  
Hugo Pacheco de Freitas Fraga ◽  
Marguerite Quoirin
Keyword(s):  
In Vitro Culture ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Culture Medium ◽  
Current Knowledge ◽  
Nodal Segments ◽  
Scale Production ◽  
Bambusa Oldhamii

ABSTRACT Bambusa oldhamii Munro is a fast-growing species of woody bamboo with strong commercial appeal. In Brazil, the use of this species is limited, mainly due to the low availability of seedlings for commercial plantations. Micropropagation is a technique used for the large scale production of seedlings, but protocols for the establishment of aseptic cultures are hampered by the presence of endophytic contamination. This study aimed to develop an in vitro establishment protocol for B. oldhamii, as well as to make the molecular identification of fungi associated with the explants used. Nodal segments of adult plants grown in the field were used as explants. This material was submitted to two experiments carried out to evaluate the effect of 6-benzylaminopurine (BAP) on multiplication and of Plant Preservative Mixture (PPMTM) as a disinfectant. In the first one, 10 µM, 15 µM or 20 µM of BAP were combined with 1 mL L-1, 2 mL L-1 or 3 mL L-1 of PPMTM; while the second one used 0 µM, 2.5 µM, 5 µM or 7.5 µM of BAP with 4 mL L-1 of PPMTM, both added to MS culture medium. After 21 days of culture, the use of 4 mL L-1 of PPMTM inhibited the bacterial growth and reduced fungal contamination. The addition of BAP to the culture medium above 10 µM inhibited the formation and growth of new shoots, while additions of less than 7,5 µM had no effect. The molecular identification of the endophytic fungi isolated during the in vitro culture indicated the presence of numerous fungal species, increasing the current knowledge about the diversity of fungi associated with bamboo.

Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers

2012 ◽  
Vol 35 (2) ◽  
pp. 419-430 ◽  
Author(s):  
Sharbati R. Singh ◽  
Sunita Dalal ◽  
Rohtas Singh ◽  
A. K. Dhawan ◽  
Rajwant K. Kalia
Keyword(s):  
Genetic Fidelity ◽  
Dendrocalamus Asper

scholarly journals The increase in in vitro shoot multiplication rate of Dendrocalamus asper (Schult. f.) Back. ex Heyne.

2012 ◽  
Vol 34 (3se) ◽  
Author(s):  
Tran Trong Tuan ◽  
Huynh Le Thien Tu ◽  
Do Dang Giap ◽  
Thai Xuan Du
Keyword(s):  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Dendrocalamus Asper

scholarly journals Somatic embryogenesis from young inflorescences of the giant bamboo Dendrocalamus asper (Schult f.) Backer ex Heyne

2021 ◽  
Author(s):  
Thiago Sanches Ornellas ◽  
Yohan Fritsche ◽  
Edison Cardona Medina ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Culture Media ◽  
Basal Medium ◽  
Dendrocalamus Asper ◽  
Bamboo Plant ◽  
Embryogenic Callus Induction

Abstract Bamboos are an important worldwide non-timber forest product with current rising interest due to their environmentally friendly applications. Besides the consolidated uses of the sweet shoots and culms for structural uses, Dendrocalamus asper is an imposing ornamental bamboo for horticulture. The present work aimed to establish in vitro calli culture and plant regeneration through somatic embryogenesis starting from young inflorescences of the giant bamboo, D. asper. Pre-anthesis inflorescences were collected, disinfested, and subjected to callus induction on MS basal medium supplemented by 0 µM, 9 µM, 18 µM, 27 µM, and 36 µM of 2,4-D in combination with 9 µM of 2-iP or 9 µM Kin. The different obtained calli types were characterized and subcultured in 0 µM, 4.5 µM, 9 µM, and 18 µM of 2,4-D in combination with 9 µM of both cytokinins for multiplication and differentiation. Additionally, the explant incision and its inoculation orientation onto culture media were tested for callus induction improvement. The 2,4-D was essential for callus induction, and its combination with both cytokinins resulted in embryogenic callus induction and further somatic embryos regeneration. The subsequent reduction of this auxin to 4.5 µM resulted in somatic embryo maturation. Somatic embryos transferred to a plant growth regulator-free medium resulted in plantlet conversion. The present work showed the feasibility of using inflorescences as explants and the efficiency of using the 2-iP in combination with 2,4-D to callus induction and in vitro bamboo plant regeneration through somatic embryogenesis.

Effects of Vitrofural, BAP and meta-Topolin in the in vitro culture of Dendrocalamus asper

2017 ◽  
pp. 285-292 ◽  
Author(s):  
T.S. Ornellas ◽  
D. Werner ◽  
D.F. Holderbaum ◽  
R.F. Scherer ◽  
M.P. Guerra
Keyword(s):  
In Vitro Culture ◽  
Dendrocalamus Asper

scholarly journals A Concise Review of Dendrocalamus asper and Related Bamboos: Germplasm Conservation, Propagation and Molecular Biology

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1897
Author(s):  
Anis Adilah Mustafa ◽  
Mohammad Rahmat Derise ◽  
Wilson Thau Lym Yong ◽  
Kenneth Francis Rodrigues
Keyword(s):  
Molecular Biology ◽  
Germplasm Conservation ◽  
Future Research ◽  
Rapid Multiplication ◽  
Economic Significance ◽  
Dendrocalamus Asper ◽  
Subsequent Propagation ◽  
Available Information ◽  
Agro Forestry

Bamboos represent an emerging forest resource of economic significance and provide an avenue for sustainable development of forest resources. The development of the commercial bamboo industry is founded upon efficient molecular and technical approaches for the selection and rapid multiplication of elite germplasm for its subsequent propagation via commercial agro-forestry business enterprises. This review will delve into the micropropagation of Dendrocalamus asper, one of the most widely cultivated commercial varieties of bamboo, and will encompass the selection of germplasm, establishment of explants in vitro and micropropagation techniques. The currently available information pertaining to molecular biology, DNA barcoding and breeding, has been included, and potential areas for future research in the area of genetic engineering and gene regulation have been highlighted. This information will be of relevance to both commercial breeders and molecular biologists who have an interest in establishing bamboo as a crop of the future.

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