The increase in in vitro shoot multiplication rate of Dendrocalamus asper (Schult. f.) Back. ex Heyne.

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

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High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

Acta Agronomica Hungarica ◽

10.1556/aagr.50.2002.2.11 ◽

2002 ◽

Vol 50 (2) ◽

pp. 191-195 ◽

Cited By ~ 4

Author(s):

K. Magyar-Tábori ◽

J. Dobránszki ◽

E. Jámbor-Benczúr

Keyword(s):

Shoot Proliferation ◽

Shoot Multiplication ◽

Shoot Length ◽

Multiplication Rate ◽

Apple Cultivar ◽

High Concentration ◽

Suppression Effect ◽

High Concentrations ◽

In Vitro Shoot Proliferation

The in vitro shoot multiplication of apple cv. Jonagold was tested on media containing benzyladenine, benzyladenine riboside or meta-topolin in different concentrations (from 0.0 to 5.0 mg l-1). The optimal concentration for the best multiplication varied according to the type of cytokinin. The highest multiplication rate (on average 6.9 and 5.9 new shoots per explant) was achieved using 5.0 mg l-1 meta-topolin or 2.0 mg l-1 benzyladenine riboside. The longest shoots were formed on media containing benzyladenine riboside at a concentration of 0.5 mg l-1. The length of newly developed shoots was strongly suppressed by high concentrations of different cytokinins, but the suppression effect of a high concentration of meta-topolin on shoot length was less than that of benzyladenine or benzyladenine riboside. In this study meta-topolin and benzyladenine riboside proved to be effective cytokinins to induce adequate shoot proliferation, while benzyladenine was the least active cytokinin

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Micropropagation of Cyclopia genistoides, an Endemic South African Plant of Economic Importance

Zeitschrift für Naturforschung C ◽

10.1515/znc-2012-1-209 ◽

2012 ◽

Vol 67 (1-2) ◽

pp. 65-76 ◽

Cited By ~ 1

Author(s):

Adam Kokotkiewicz ◽

Maria Luczkiewicz ◽

Anna Hering ◽

Renata Ochocka ◽

Krzysztof Gorynski ◽

...

Keyword(s):

South African ◽

Sucrose Concentration ◽

Shoot Multiplication ◽

Potassium Nitrate ◽

Economic Importance ◽

Medium Composition ◽

Root Induction ◽

Multiplication Rate ◽

Shoot Cultures

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 μM 6-(γ,γ-dimethylallylamino)purine (2iP) and 1.0 μM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 ± 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 μM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 μM indole-3-acetic acid (IAA) and 260.25 μM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the fl avanone hesperidin, characteristic of wild-growing shrubs.

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In vitro Regeneration and Callogenesis of Libidibia ferrea

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i430495 ◽

2020 ◽

pp. 14-24

Author(s):

Daniel da Silva ◽

Angela Maria Imakawa ◽

Kamylla Rosas Vieira Guedes ◽

Flávio Mauro Souza Bruno ◽

Paulo de Tarso Barbosa Sampaio

Keyword(s):

Culture Media ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Light Sources ◽

Multiplication Rate ◽

Medicinal Species ◽

Efficient System ◽

Blue Led ◽

Friable Callus

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

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IMPROVED MULTIPLICATION OF MATURE HAZELNUT (CORYLUS AVELLANA L.) IN VITRO

HortScience ◽

10.21273/hortsci.27.6.693b ◽

1992 ◽

Vol 27 (6) ◽

pp. 693b-693

Author(s):

Xiaoling Yu ◽

Barbara M. Reed

Keyword(s):

Carbon Sources ◽

Basal Medium ◽

Shoot Multiplication ◽

Multiplication Rate ◽

Shoot Cultures ◽

Mature Trees ◽

Medium Carbon ◽

Prolonged Culture ◽

Corylus Avellana L

Multiplication and elongation of shoot cultures established from mature trees of hazelnut cvs. Nonpareil and Tonda Gentile Romana were affected by changes in basal medium, carbon source and concentration, cytokinin and agar concentration. Explants on DKW medium produced significantly more shoots than those on Anderson medium or modified woody plant medium for chestnut. Explants on DKW medium with 3% glucose or fructose gave more and longer shoots than those with the other carbon sources. Cytokinins 6 benzylaminopurine (BA) and zeatin were more effective in producing shoots than kinetin and 2iP. On BA supplemented medium, the best multiplication rate was obtained with 1.5 - 2.0 mg/l. Explants grown on 0.4% agar produced more shoots than those on 0.6%, however, prolonged culture on 0.4% agar caused vitrification of lower parts of the plants. Shoot multiplication rates of these two cultivars were similar, but `Nonpareil' produced longer shoots than `Tonda Gentile Romana'.

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312 Influence of BAP and CPPU on the in Vitro Shoot and Bud Proliferation of Apple Rootstocks M.111 and M.7

HortScience ◽

10.21273/hortsci.35.3.445f ◽

2000 ◽

Vol 35 (3) ◽

pp. 445F-446

Author(s):

Adriano N. Nesi ◽

Gerson R. de L. Fortes ◽

Jono B. da Silva ◽

Adriana C. de M. Dantas

Keyword(s):

Callus Formation ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Temperate Climate ◽

Similar Response ◽

Multiplication Rate ◽

Apple Rootstocks ◽

Shoot Base ◽

Better Than

This work aims to verify the effect of BAP (6-benzyladenine purine) and CPPU (forchlofenuron) on the in vitro shoot proliferation of apple rootstock cultivars M.111 and M.7 under different concentrations. The experiment was carried out in the tissue culture laboratory at Embrapa Temperate Climate in Pelotas, RS, Brazil. As initial explants, microcuttings were used from in vitro culture. The treatments consisted of the combination of two cultivars with cytokinins and six differents concentrations (0.0, 1.5, 3.0, 4.5, and 6.0 μMol). The explants were inoculated in 250-mL flasks with 40 mL MS medium with agar (7.0 g·L-1), myo-inositol (100.0 g·L-1), NAA (0.005 mg·L-1), and sucrose (40.0 g·L-1). The pH was adjusted to 5.9 before autoclaving. After inoculations the culture was kept for 50 days under 25 ± 2 °C, 16-h photoperiod, and 19 μMol·m-2·s-1 radiation. CPPU performed better than BAP for cultivar M.111 and it had similar response for cultivar M.7 as bud and shoot multiplication and multiplication rate is concerned. The BAP increased the number of shoots with higher length and with no callus formation in the shoot base, contrary to CPPU. The most efficient concentrations were 4.7 and 5.5 μMol for CPPU and BAP, respectively.

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Peningkatan laju multiplikasi tunas dan keragaan planlet Stevia rebaudiana pada kultur in vitro Increasing shoot multiplication rate and plantlet vigor of Stevia rebaudiana in vitro culture

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v79i2.59 ◽

2016 ◽

Vol 79 (2) ◽

Author(s):

. SUMARYONO ◽

Masna Maya SINTA

Keyword(s):

Light Intensity ◽

Plant Growth ◽

Stevia Rebaudiana ◽

Shoot Multiplication ◽

Culture Technique ◽

Multiplication Rate ◽

Stevia Rebaudiana Bertoni ◽

Plant Grown ◽

Planting Materials

AbstractStevia (Stevia rebaudiana Bertoni) is a natural zero-calorie sweetener plant grown in a high population density.Tissue culture technique is useful for rapid mass propagationof plants to provide superior planting materials. Experimentswere conducted to increase growth and multiplication ofshoots and vigor of plantlets of stevia. Explants used wereapical and axillary buds from plantlets grown on MS mediumwithout plant growth regulators. Combinations of BA andIAA at different concentrations were used for shoot growthand multiplication, whereas plant growth retardants(ancymidol and paclobutrazol) and light intensity were usedfor plantlet vigor. The results showed that stevia explantscultured on MS medium without plant growth regulatorsproduced the highest shoots (4.5 cm) with two shoots perexplant. The best multiplication rate of shoots were found onMS medium added with 1.13 mg/L BA combined with0.35 mg/L IAA which produced on average 4.5 shoots and11.9 nodes per initial explant. Ancymidol and paclobutrazolconcentrations affected significantly growth and vigor ofstevia plantlets. Increasing the concentration of ancymidoland paclobutrazol decreased plantlet height and biomassfresh weight, but increased stem diameter. Paclobutrazol at0.1 mg/L was the best treatment to increase the vigor ofstevia plantlets. Light intensity at 20 µmol/m 2 /s gave betterplantlet vigor than other light intensities. It can be concludedthat multiplication of stevia shoots should be grown on MSmedium supplemented with 1.13 mg/L BA + 0.35 mg/L IAAand the vigor of the shoots can be increased by culturing onMS medium containing 0.1 mg/L paclobutrazol underfluorescence lamps with 20 µmol/m 2 /s light intensity.AbstrakStevia (Stevia rebaudiana Bertoni) adalah tanamanpemanis alami nir-kalori yang ditanam dengan kerapatanpopulasi yang sangat tinggi. Teknik kultur jaringan dapatdigunakan untuk perbanyakan tanaman secara massal dancepat untuk menyediakan bahan tanam unggul. Penelitiantelah dilakukan untuk meningkatkan pertumbuhan danmultiplikasi tunas dan keragaan planlet stevia. Eksplan yangdigunakan adalah tunas pucuk dan tunas samping dari planletyang ditumbuhkan pada medium MS tanpa zat pengaturtumbuh. Kombinasi BA dan IAA dengan konsentrasi yangberbeda digunakan untuk pertumbuhan dan multiplikasitunas, sedangkan zat penghambat tumbuh (ansimidol danpaklobutrazol) serta intensitas cahaya digunakan untukkeragaan planlet. Hasil penelitian menunjukkan bahwaeksplan stevia yang ditumbuhkan pada medium MS tanpa zatpengatur tumbuh menghasilkan tunas paling tinggi (4,5 cm)dengan dua tunas per eksplan. Multiplikasi tunas terbaikdiperoleh pada medium dengan BA 1,13 mg/L yangdikombinasikan dengan IAA 0,35 mg/L yang menghasilkan4,5 tunas dan 11,9 ruas per eksplan awal. Konsentrasiansimidol dan paklobutrazol berpengaruh nyata terhadappertumbuhan dan keragaan planlet stevia. Meningkatnyakonsentrasi ansimidol dan paklobutrazol menurunkan tinggiplanlet dan bobot basah biomassa, tetapi meningkatkandiameter batang. Paklobutrazol pada konsentrasi 0,1 mg/Lmerupakan perlakuan terbaik untuk meningkatkan keragaanplanlet stevia. Intensitas cahaya pada 20 µmol/m 2 /detikmemberikan keragaan planlet yang lebih baik dibandingkanintensitas cahaya yang lain. Dapat disimpulkan bahwamultiplikasi tunas stevia sebaiknya dilakukan pada mediumMS ditambah BA 1,13 mg/L + IAA 0,35 mg/L dan keragaanplanlet dapat ditingkatkan dengan menanam planlet padamedium MS ditambah paklobutrazol 0,1 mg/L di bawahlampu fluoresen dengan intensitas cahaya 20 µmol/m 2 /detik.

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In vitro Clonal Multiplication of Two Grape (Vitis spp.) Rootstock Genotypes

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v28i1.37193 ◽

2018 ◽

Vol 28 (1) ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

M Alizadeh ◽

SK Singh ◽

VB Patel ◽

PS Deshmukh

Keyword(s):

In Vitro Regeneration ◽

Shoot Multiplication ◽

Nodal Segments ◽

Multiplication Rate ◽

Culture Initiation ◽

Single Node ◽

Clonal Multiplication ◽

Rooting Medium ◽

In Vitro Performance

Single node segments were used to initiate in vitro cultures in two grape rootstocks namely, Dogridge (Vitis champini) and H-144 (Vitis vinifera × V. labrusca). Culture establishment was enhanced using different growth regulators, while BAP was found essential for culture initiation in both genotypes. Less success (38.31%) was obtained in culture establishment of H-144 but it exhibited better vegetative growth and rooting and ex vitro performance as compared to Dogridge. Higher shoot multiplication rate (12 micro-cuttings per culture) was recorded in H-144 while only 9 micro-cuttings per subculture were registered in Dogridge. Addition of activated charcoal to the rooting medium was found beneficial with enhancement of rooting and reduction in time to root initiation in both genotypes. The results suggested that multiplication of these two grape rootstock genotypes can be carried out efficiently by means of direct in vitro regeneration using nodal segments. In vitro performance of these two genotypes was also compared during different stages of micropropagation.Plant Tissue Cult. & Biotech. 28(1): 1-11, 2018 (June)

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The effect of BA and GA3 on the shoot multiplication of in vitro cultures of Polish wild roses

Folia Horticulturae ◽

10.2478/v10245-011-0022-5 ◽

2011 ◽

Vol 23 (2) ◽

pp. 145-149 ◽

Cited By ~ 5

Author(s):

Bożena Pawłowska

Keyword(s):

Relative Humidity ◽

Ornamental Plants ◽

Shoot Multiplication ◽

In Vitro Cultures ◽

Multiplication Rate ◽

Ms Medium ◽

Naturally Occurring ◽

Dog Rose ◽

The University

The effect of BA and GA3on the shoot multiplication ofin vitrocultures of Polish wild rosesThe experiment was conducted using five species of roses naturally occurring in Poland:Rosa agrestis(fieldbriar rose),R. canina(dog rose),R. dumalis(glaucous dog rose),R. rubiginosa(sweetbriar rose), andR. tomentosa(whitewooly rose), from thein vitrocollection of the Department of Ornamental Plants of the University of Agriculture in Kraków. We examined the effect of cytokinin BA (1-10 μM) added to an MS medium (Murashige and Skoog 1962) on auxiliary shoot multiplication. The second group of test media contained BA (1-5 μM) and gibberellin GA3(0.3-1.5 μM). The cultures were maintained at a phytotron temperature of 23/25°C (night/day), 80% relative humidity, with a 16-hour photoperiod and PPFD of 30 μmol m-2s-1, and cultured in five-week cycles. The highest multiplication rate was obtained forR. caninaandR. rubiginosa(4.1 shoots per one explant) andR. dumalis(2.9 shoots per one explant), when shoots were multiplied on an MS medium supplemented with 1 μM BA and 1.5 μM GA3. Multiplication was the weakest inRosa tomentosaindependent of the medium used.

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽

10.3390/plants10051010 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1010

Author(s):

Eucario Mancilla-Álvarez ◽

Juan Antonio Pérez-Sato ◽

Rosalía Núñez-Pastrana ◽

José L. Spinoso-Castillo ◽

Jericó J. Bello-Bello

Keyword(s):

Culture Medium ◽

Solid Medium ◽

Survival Rates ◽

Shoot Multiplication ◽

Control Treatment ◽

Multiplication Rate ◽

Solid Culture ◽

Solid Culture Medium ◽

Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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