Fresh leaf extract’s efficacy of twelve medicinal plants against Culex quinquefasciatus mosquito larvae

2020 ◽  
Vol 8 (2) ◽  
pp. 606-617
Author(s):  
M. Junayed ◽  
T. Akter ◽  
S. Ahmad
1996 ◽  
Vol 38 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Ricardo Miguel Costa de Freitas ◽  
Mágda de Abreu Faria ◽  
Stênio Nunes Alves ◽  
Alan Lane de Melo

The effects of ivermectin, a semi-synthetic drug widely used for treatment of livestock parasitic diseases, were observed on Culex quinquefasciatus larvae. Toxic effects and mortality evaluation were carried out after 5, 15, 30 and 60 minutes of exposure to 1, 5 or 10 ppm of ivermectin solutions. Observations were made 24 and 48 hours after the beginning of the experiment, and loss of mobility, progressive paralysis and high mortality of larvae were recorded. The observed effects of ivermectin on the mosquito larvae is probably correlated with chloride channel activation on cell membranes.


2003 ◽  
Vol 17 (2) ◽  
pp. 158-164 ◽  
Author(s):  
V. Corbel ◽  
F. Chandre ◽  
F. Darriet ◽  
F. Lardeux ◽  
J-M. Hougard

2021 ◽  
Vol 3 (1) ◽  
pp. 77-85
Author(s):  
Yahya A. Derua ◽  
Eliningaya J. Kweka ◽  
William N. Kisinza ◽  
Guiyun Yan ◽  
Andrew K. Githeko ◽  
...  

Background: The efficacy of Bacillus thuringiensis var. israelensis (Bti) is affected by several factors including the species of the mosquito. Mosquito larvae of different species are found to coexist in larval breeding habitats. This study evaluated whether the coexistence between Anopheles gambiae and Culex quinquefasciatus affect the larvicidal activity of Bti. Methods: Two parallel larval bioassay experiments were conducted to test A. gambiae sensu stricto (s.s) and C. quinquefasciatus larvae susceptibility to Bti. They were followed by three parallel bioassays in which A. gambiae s.s and C. quinquefasciatus larvae were mixed in different proportions such that the earlier species contributed three quarters, half and a quarter of the larvae in each testing cup respectively. In each bioassay, six Bti concentrations were tested in four replicates and repeated on three different days. Larvae mortality was scored 24 hours after application of Bti and subjected to Probit analysis. Results: C. quinquefasciatus was significantly more susceptible to Bti than A. gambiae s.s at both lethal concentration values (LC50 and LC95). In coexisting scenario, LC50 of Bti was significantly lower when the proportion of C. quinquefasciatus exceeded 50%. No significant variation in susceptibility to Bti was observed at LC95 in any proportion of coexistence between the two species. Conclusion: The findings show that larvae of C. quinquefasciatus were significantly more susceptible to Bti than those of A. gambiae s.s. Moreover, when larvae of the two species coexisted, there was a general trend of increase in sensitivity to Bti with higher proportion of C. quinquefasciatus. Although this increase in sensitivity of coexisting larvae to Bti is worth noting, our findings suggest that it will not impact larval control where A. gambiae s.s and C. quinquefasciatus coexist.


2018 ◽  
Vol 27 (2) ◽  
pp. 135-144
Author(s):  
Humayun Reza Khan ◽  
Nigar Sultana ◽  
Prova Sharmin

Eucalyptus camaldulensis was assayed to evaluate the larvicidal efficacy of its leaf extracts against the 3rd instar larvae of Culex quinquefasciatus at five dose concentrations (viz. 0.25, 0.50, 0.75, 1.0, 1.25, 1.5, 2.0, 2.5 and 3.0) in five solvents (viz. chloroform, ethanol, di-chloromethane, acetone and water) based extracts after 24 hrs exposure. Among all the extracts the shade dried chloroform based leaf extracts showed the highest larvicidal efficacy (viz. 42.67, 56.67, 68.0, 92.67 and 100%) at low dose concentrations of 0.25, 0.50, 0.75, 1.0 and 1.25, respectively while the sun dried water based leaf extracts showed the lowest larvicidal efficacy (viz. 21.33, 30.67, 48.67, 58.0 and 74.0%) at high dose concentrations of 1.0, 1.5, 2.0, 2.5 and 3.0, respectively among the ten different experimental conditions. The relative potency of ten types of crude leaf extracts of E. camaldulensis against the mosquito larvae are shown as follows in decreasing order on the basis of LC50 value : Shade dried chloroform based leaf extract (0.356 mg/ml) > sun dried chloroform based leaf extract (0.400 mg/ml) > shade dried di-chloromethane based leaf extract (0.411 mg/ml) > sun dried dichloromethane based leaf extract (0.579 mg/ml) > shade dried ethanol based leaf extract (0.736 mg/ml) > sun dried ethanol based leaf extract (0.817 mg/ml) > shade dried acetone based leaf extract (1.000 mg/ml) > sun dried acetone based leaf extract (1.251 mg/ml) > shade dried water based leaf extract (1.807 mg/ml) > Sun dried water based leaf extract (2.020 mg/ml).The effectiveness of the shade dried leaf extracts was shown higher than the sun dried leaf extracts under comparable condition. No mortality was observed in control treatment. The study revealed that these leaf extracts have the potency to consider as an effective larvicidal agent. It is an alternative source for developing a novel larvicide for controlling the mosquito species. Dhaka Univ. J. Biol. Sci. 27(2): 135-144, 2018 (July)


2018 ◽  
Vol 12 (1) ◽  
pp. 33-38
Author(s):  
Arif Nur Muhammad Ansori ◽  
Hebert Adrianto ◽  
Hamidah Hamidah

Abstract The development of resistance to chemical insecticides against mosquitoes has beenconsidered as a setback in vector control. This study was aimed to identify LethalConcentration 90% (LC90) of polar fraction extract from  Citrus hystrix and Citrusaurantifolia leaves as biolarvicide against mosquito larvae after 24 hours of exposure.This study was an experimental research based on a Completely Randomized Design(CRD). The polar fraction extract of C. hystrix and C. aurantifolia  were tested in concentrations of 0 ppm, 500 ppm, 1375 ppm, 2250 ppm, 3125 ppm, and 4000 ppm againstthe 3rd instar larvae of  Culex quinquefasciatus. Each treatment was done in five independent replications. The numbers of deceased mosquito larvae were determinedafter 24 hours of treatment and analyzed by probit. The results showed that the polarfraction extract of  C. hystrix and C. aurantifolia  have bio-larvicidal activity against the 3rdinstar larvae of Cx. quinquefasciatus at LC90 of 1,653 ppm and 2,797 ppm, respectively. In conclusion, the polar fraction extract of C. hystrix leaf is more toxic than C. aurantifolialeaf's against  Cx. quinquefasciatus larvae. Abstrak Perkembangan resistensi terhadap insektisida kimia di antara spesies nyamuk telahdianggap sebagai kemunduran dalam pengendalian vektor. Penelitian ini bertujuanuntuk mengetahui Lethal Concentration 90% (LC90 ) ekstrak fraksi polar daun  C. hystrix dan C. aurantifolia sebagai biolarvasida terhadap larva nyamuk setelah paparan 24 jam. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL).Ekstrak fraksi polar daun C. hystrix dan C. aurantifolia  diuji dengan konsentrasi 0 ppm,500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm terhadap larva nyamuk Cx.quinquefasciatus instar III. Setiap konsentrasi dilakukan replikasi sebanyak lima kali.Jumlah larva nyamuk yang mati dihitung setelah 24 jam. Setelah itu, data dari larvanyamuk yang mati dianalisis menggunakan probit. Hasil penelitian ini mengungkapkanbahwa ekstrak fraksi polar daun C. hystrix dan C. aurantifolia memiliki aktivitas sebagai biolarvasida terhadap larva nyamuk Cx. quinquefasciatus instar III dengan LC 90= 1.653  ppm dan 2.797 ppm, secara berurutan. Kesimpulan dari penelitian ini adalah ekstrakfraksi polar dari daun C. hystrix lebih toksik dibandingkan dengan  C. aurantifoliaterhadap larva nyamuk instar III.  


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