​Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs with White Muscle Disease

2021 ◽  
Author(s):  
Kıvanç Irak ◽  
Handan Mert ◽  
Nihat Mert ◽  
Nesrullah Ayşin ◽  
İnci Doğan Söğütlü ◽  
...  
Keyword(s):  
Vitamin E ◽  
Total Protein ◽  
Biochemical Parameters ◽  
White Muscle ◽  
Statistical Significance ◽  
Blood Parameters ◽  
Muscle Disease ◽  
Tissue Samples ◽  
White Muscle Disease ◽  
Blood Samples

Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups.

Effects of Selenium and Vitamin E on White Muscle Disease

Science ◽  
1958 ◽  
Vol 128 (3331) ◽  
pp. 1090-1090 ◽  
Author(s):  
O. H. MUTH ◽  
J. E. OLDFIELD ◽  
L. F. REMMERT ◽  
J. R. SCHUBERT
Keyword(s):  
Vitamin E ◽  
White Muscle ◽  
Muscle Disease ◽  
White Muscle Disease

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals INFLUENCE OF SELENIUM ON THE SELENIUM CONTENTS OF HAIR AND ON THE INCIDENCE OF NUTRITIONAL MUSCULAR DISEASE IN BEEF CATTLE

1965 ◽  
Vol 45 (3) ◽  
pp. 197-202 ◽  
Author(s):  
M. Hidiroglou ◽  
R. B. Carson ◽  
G. A. Brossard
Keyword(s):  
Vitamin E ◽  
Beef Cattle ◽  
White Muscle ◽  
Muscle Disease ◽  
Sulphur Content ◽  
Selenium Content ◽  
Northern Ontario ◽  
White Muscle Disease ◽  
Muscular Disease ◽  
Low Selenium

In this study, the selenium content of forage from farms in northern Ontario where nutritional muscular disease is very severe was less than 0.1 p.p.m. Variations in the sulphur content of forage did not appear to affect the occurrence of the disease. A combination of vitamin E and selenium given to pregnant dams or directly to calves was completely effective in preventing the disease. Data on the selenium content of the hair of dams and calves are presented. Calves, sick or dead from white muscle disease, were born to cows with low selenium content of the hair (range 0.06–0.23 p.p.m.). No white muscle disease was observed in calves from dams with selenium content of the hair above 0.25 p.p.m. Selenium content of the hair was generally greater in the selenium-treated calves or those born to selenium-treated dams. It seems probable that the selenium content of hair may be a helpful factor in diagnosing white muscle disease.

Efficacy of Selenium and Vitamin E Injections in the Prevention of White Muscle Disease in Young Swine

1973 ◽  
Vol 36 (6) ◽  
pp. 1104-1108 ◽  
Author(s):  
D. C. Mahan ◽  
J. E. Jones ◽  
J. H. Cline ◽  
R. F. Cross ◽  
H. S. Teague ◽  
...  
Keyword(s):  
Vitamin E ◽  
White Muscle ◽  
Muscle Disease ◽  
White Muscle Disease

Vitamin E and selenium status of sheep during autumn in Western Australia and its relationship to the incidence of apparent white muscle disease

2007 ◽  
Vol 47 (5) ◽  
pp. 535 ◽  
Author(s):  
C. L. White ◽  
L. Rewell
Keyword(s):  
Vitamin E ◽  
Whole Blood ◽  
White Muscle ◽  
Selenium Deficiency ◽  
Muscle Disease ◽  
Plasma Vitamin ◽  
Vitamin E Deficiency ◽  
White Muscle Disease ◽  
Adult Sheep ◽  
Plasma Vitamin E

This paper reports on the status of vitamin E and selenium in weaner and adult sheep in Western Australia (WA) during autumn, and its relationship to the incidence of apparent white muscle disease (WMD). A survey was conducted in which blood samples were taken from 10 weaner sheep (8–12 months of age) and 10 adult ewes (3 years of age) from flocks on 38 properties within the main southern agricultural region of WA, an area carrying ~10 million sheep. Deficiency of vitamin E was defined as a plasma α-tocopherol concentration less than 0.7 mg/L. Selenium deficiency was defined as whole blood glutathione peroxidase (GPx) activity less than 50 U/L. Incidence and severity of apparent WMD were defined in terms of elevated plasma activities of creatine kinase (CK) or aspartate aminotransaminase (AST). Of the weaner flocks, 58% (22/38) had mean plasma vitamin E concentrations in the deficient range (<0.7 mg/L) and 50% (19/38) had mean CK activities above 400 U/L (mild WMD); four of these flocks had mean CK activities over 1200 U/L (severe WMD). Of all the individual weaner sheep sampled, 6% had plasma CK activities >1200 U/L. It is likely that the incidence of vitamin E deficiency would have been higher had it not been for unseasonal summer rain germinating pasture on eight of the survey farms in the northern wheat belt. Flocks from farms with an evident green flush to their pastures had higher vitamin E levels than flocks grazing dry pasture. Vitamin E deficiency was less common in adult sheep than weaner sheep, with only 16% of flocks (6/38) having mean plasma concentrations below 0.7 mg/L and 11% (4/38) with CK values above 400 U/L. Selenium deficiency was less common than vitamin E deficiency and was mostly confined to adult flocks, with only 5% (2/38) of these having mean GPx values below the critical value of 50 U/g haemoglobin. For both weaner and adult sheep classed as vitamin E deficient, CK and AST activity in plasma was best described by a combination of plasma vitamin E concentration and whole blood activity of GPx (significant linear step wise regression, P < 0.001). The present study shows that subclinical vitamin E deficiency (<0.7 mg/L) was widespread in weaner flocks in WA during autumn and that, based on the biochemical data, deficiency was associated with apparent severe muscle damage in 6% of weaner sheep sampled. Recommended strategies to treat weaner sheep showing signs of WMD include dosing with 2000–4000 mg vitamin E either by injection, oral drench or by spraying it onto supplementary grain. The level and frequency of dosing depends upon the severity of the WMD symptoms. Less is known about preventative treatment, but recent evidence suggests that providing weaner sheep with access to saltbush during autumn may prove to be a practically useful strategy. Selenium supplements should also be supplied to weaner and adult sheep in areas known to be selenium deficient.

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