Serological and molecular detection of Bovine Herpes Virus 1 infection in cattle by enzyme linked immunosorbent assay and polymerase chain reaction

2019 ◽  
Author(s):  
P. M. Jithin ◽  
Surya Sankar ◽  
Binu K. Mani ◽  
Shibu Simon ◽  
Roshin M. Reji ◽  
...  
Keyword(s):  
Herpes Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nucleotide Sequencing ◽  
Pcr Assay ◽  
Serum Samples ◽  
Tissue Samples ◽  
Glycoprotein C ◽  
Amplicon Size ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus

The present study was undertaken to assess the seroprevalence of Bovine Herpes Virus 1 (BoHV 1) in cattle in and around Thrissur district employing ELISA and to detect the presence of the virus from suspected cases of abortion in cattle using PCR assay. A total of 182 serum samples were screened using commercially available ELISA kit of which 22 were found positive for BoHV1 antibody with a seropositivity of 12.09 per cent. For the detection of BoHV1 virus in aborted cases, a PCR assay was standardised targeting glycoprotein C gene of BoHV1. Out of a total of 13 aborted foetal tissue samples, six were found to be positive with an amplicon size of 179 bp. Further, the amplicons were confirmed by nucleotide sequencing. Phylogenetic analysis of the sequences showed that our sample from Kerala grouped with isolates from India (Gujarat and UP), Brazil, Switzerland, and USA.

scholarly journals Development of a Probe Based Real Time PCR Assay for Detection of Bovine Herpes Virus-1 in Semen and Other Clinical Samples

2012 ◽  
Vol 24 (1) ◽  
pp. 16-26
Author(s):  
Basavegowdanadoddi Marinaik Chandranaik ◽  
Doddamane Rathnamma ◽  
S. S. Patil ◽  
Ramesh C. Kovi ◽  
Jyotsana Dhawan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus

scholarly journals Rats born to Brucella abortus infected mothers become latent carriers of Brucella

2011 ◽  
Vol 6 (03) ◽  
pp. 256-261 ◽  
Author(s):  
Md. Ariful Islam ◽  
Mst. Minara Khatun ◽  
Byeong-Kirl Baek
Keyword(s):  
Brucella Abortus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sprague Dawley ◽  
Pcr Assay ◽  
Serum Samples ◽  
Tissue Samples ◽  
Sd Rats ◽  
Brucella Infection ◽  
Agar Media ◽  
The Rose

Introduction: Rats are known to be infected with Brucella. Vertical transmission of brucellosis was recorded in rats. The study was performed to judge whether rats born from Brucella abortus infected mothers can act as latent carriers of Brucella infection. Methodology: Female Sprague Dawley (SD) rats were experimentally infected with B. abortus biotype 1 and subsequently bred 10 days post infection (PI). Serum samples of rats (n = 48) born from infected dams were tested using the Rose Bengal plate test (RBPT), tube agglutination test (TAT), and enzyme-linked immunosorbent assay (ELISA) at one, two and three months of age. Tissue samples were plated onto Brucella agar and blood agar media and incubated at 37°C with 5% CO2 for five to seven days for isolation of bacteria.. Results: B. abortus was isolated from 18 out of 48 rats born to infected dams, and the isolates were confirmed as B. abortus by AMOS (B. abortus, melitensis, ovis and suis) PCR assay with the production of a 498 bp PCR amplicon. Serum samples of rats (n = 48) born from infected dams were tested negative using the RBPT, TAT and ELISA at all time points. Conclusion: We conclude from the study that rats born to infected dams may become latent carriers of Brucella infection potentially providing a reservoir for future transmission.

Human herpes virus-6 (HHV-6) pneumonitis and meningitis with viraemia in an immunocompetent adult patient

2021 ◽  
Vol 14 (3) ◽  
pp. e239220
Author(s):  
Maria Alkozah ◽  
Rami Hallak ◽  
Imad Bou Akl ◽  
Aline El Zakhem
Keyword(s):  
Herpes Virus ◽  
Case Reports ◽  
Paediatric Population ◽  
Atypical Pneumonia ◽  
Virus Reactivation ◽  
Human Herpes ◽  
Serum Samples ◽  
Human Herpes Virus ◽  
Human Herpes Virus 6 ◽  
Herpès Virus

Human herpes virus-6 (HHV-6) infection is a common infection in the paediatric population and is increasingly reported in immunosuppressed adult patients. It has been reported as the causative agent of disease in few case reports in immunocompetent adults. We report herein an unusual case of HHV-6-associated viraemia, pneumonitis and meningitis in a patient who presented with dyspnoea, hypoxia, dry cough and headache. She was treated for atypical pneumonia with no improvement. Meningitis was suspected as headache kept worsening. HHV-6B was detected by PCR in the cerebrospinal fluid, and subsequently, in the bronchoalveolar lavage and serum samples. Studies were negative for the most common primary and secondary immunodeficiency syndromes, and serology could not be performed to differentiate virus reactivation from a primary infection. The patient was successfully treated with ganciclovir and had no residual sequelae.

The role of goats as reservoir hosts for bovine herpes virus 1 under field conditions

2018 ◽  
Vol 51 (4) ◽  
pp. 753-758 ◽  
Author(s):  
Sibel Gür ◽  
Nural Erol ◽  
Orhan Yapıcı ◽  
Mehmet Kale ◽  
Mehmet Tolga Tan ◽  
...  
Keyword(s):  
Herpes Virus ◽  
Field Conditions ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus ◽  
Reservoir Hosts

FIRST RESULTS OF APPLICATION OF AN ENZYME IMMUNOASSAY IN THE SEROLOGICAL DIAGNOSTICS OF INFECTION CAUSED BY HERPESVIRUS TYPE 7

2019 ◽  
Vol 64 (9) ◽  
pp. 530-535
Author(s):  
Seifaddin Gashimovich Mardanly ◽  
V. A. Arsenyeva ◽  
A. S. Avdonina ◽  
E. A. Amelina ◽  
S. S. Mardanly
Keyword(s):  
Herpes Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Herpesvirus Type ◽  
Test Kit ◽  
First Results ◽  
Specific Igg ◽  
Herpès Virus ◽  
Serological Diagnostics ◽  
Human Herpes Virus Type

HHV-7 (Human Herpes virus type 7) is a relatively recently discovered, ubiquitous beta herpes virus. In Russia, the diagnosis of HHV-7 infection is carried out by PCR, which determines the DNA in serum or plasma, if there are clinical indications. Laboratory serological tests for HHV-7 are not performed. Currently, in the scientific literature, information on clinical studies of HHV-7 infection, its epidemiology, and its role in the etiopathogenesis of various diseases is insufficient. This article presents the results of the development of a test kit “ELISA-HHV-7-IgG”, designed to detect specific IgG to HHV-7 by enzyme-linked immunosorbent assay (ELISA). Studies of the sensitivity and specificity of the developed test kit, including potential cross-reactive samples positive for other herpesvirus infections, were carried out. In the context of serological diagnostics, we studied samples of the sera of children (n = 167) and old people (n = 238) for the presence of IgG to HHV-7 with confirmation of the results in the indirect immunofluorescence reaction and the immune blotting reaction. The research results are evidence of the possibility of using the developed test kit for serological diagnosis of HHV-7 infection.

scholarly journals Dynamics of individual animal Bovine Herpes Virus-1 antibody status on 9 commercial dairy herds

2012 ◽  
Vol 93 (1) ◽  
pp. 143-149 ◽  
Author(s):  
T. Geraghty ◽  
R. O’Neill ◽  
S.J. More ◽  
L. O’Grady
Keyword(s):  
Herpes Virus ◽  
Dairy Herds ◽  
Individual Animal ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus
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